Don

High-Level Expression of Peanut Allergens Affected by Rare Codon Usage

Biochemical & Molecular Allergology, Research Center Borstel, Germany By using Stratagenes BL21-CodonPlus(DE3)-RIL cells * withextra copies of E. coli, argU, ileY, and leuW tRNA genes,it is possible to attain high-level expression...

Restriction Endonucleases Undergo Rigorous QC Testing to Ensure Optimal Performance

Stratagene Restriction endonucleases are used in many molecular biology protocols,making the functional purity of critical importance. Each restriction enzymepreparation must be free of contaminants that can potentiall...

Codon Bias-Adjusted BL21 Derivatives for Protein Expression

Stratagene BL21-Gold cells * called the BL21-CodonPlus -RILseries** of strains, which contain extra copies of the E. coli argU,ileY, and leuW tRNA genes. This mo...

New BL21-CodonPlus Cells Correct Codon Bias in GC-Rich Genomes

Carsten-Peter Carstens Julie Bonnardel Anna Waesche Ronda Allen Stratagene Codon bias is a significant obstacle for efficient expression ofheterologous genes in E. coli hosts. In GC-rich genomes, such as mammals,rare arginine codons (AGG or AGA) and the proline codon (CCC) most fre...

Entrepreneurs Dont RequireVenture capital, high-tech or government programs

RACINE -- Developing entrepreneurial eco-systems -- where the seeds of start-up and early stage ventures take root and bloom -- does NOT require venture capital, high technologies, and new government programs. It can happen anywhere if three ingredients are brought together, a national expert on entrepreneurial development told an audience here last week. Dr. Erik R. Pages presentation,...

Utilization of Donkey Anti-Rabbit PS SPA Imaging Beads in the quantitative measurement of cAMP in cells

SPA Imaging Beads • LEADSeeker • cAMP • receptor binding assay SPA Imaging Assays are homogeneous immunoassays based on the coupling of one assay component to an SPA Imaging Bead in order to generate a signal. Polystyrene (PS) SPA Imaging Beads coated with donkey anti-rabbit antibody enable the immobilization of...

Visions: Innovating like Madonna and Willie Nelson (and Steve Jobs)

Not just to manufacturers, who he will be addressing at the state's annual Manufacturing Matters conference</...

What citizens don't ask political candidates

But when you spend time talking with ordinary citizens about problems tha...

Donley resigns as WiCell director

Donley, who was serving as WiCell's executive director and has served as general counsel to WiCell's parent, the Wisconsin Alumni...

Donley has growth plans for stem cell bank

Donley, who led litigation and patent-drafting e...

Broadband blues: Don't fall for Lightspeed hype

In the great race for global competitiveness, economic development equals broadband connectivity and broadband connectivity equals jobs. The fuel for broadband connectivity is the need for speed. Thats all the consumer need...

Don't forget UW's role in economic development

Or those candidates ca...

Why companies don't get invited to the DEMO conference

One question that always swirls around the conference comes from inquiring minds that want to know what...

Patent enforcement: What you don't know might hurt you

You or your company may have no patents and, generally, little perceived need to be concerned with patent matters. However, there is a growing group of entities that consider enforcing patents to...

3M donates $1.6 million to UW-Madison engineering building

The gift will take the form of $1.4 million in cash and $200,000 in 3M multimedia...

Women and Capital: Two words that dont always marry

Whats the market for women entrepreneurs? The market is good for their ideas. Census data show that the number of women-owned businesses and the revenue from them has increased faster than the number of all businesses combined. Women-owned businesses are less likely to fail, too, experts say. Finding money to back a woman-owned business, howevernow, that's a different...

Family reunion? Don't forget your business plan

Several years ago, while attending a conference on equity capital, the category of financing labeled "friends, family and fools" drew more than a few snickers from the participants. It turns out this "informal" source of capital, along with the entrepreneur's personal funds is nothing to laugh about, according to the most recent Global...

Schrage: Don't think of distance learning as just automation

Schrage, a research associate at MIT Media Lab and...

GE promotes Milwaukee-area CEO to London position

GE announced on Thursday that Joe Hogan, head of GE Healthcare Technologies in Waukesha, will become president and CEO of Hogan will succeed Bill Castell as head of GE Healthcare in 2006. Hogan has served as president and CEO of the Wauk...

$30m of industrial software donated to Marquette University

The software will be used for...

Don't forget building relationships when building business

Relationships are very important to succeed both personally and professionally. You should make sure to spend time building alliances with other people in the business world and your community. Once these partnerships are built, you will be able leverage them to help you fulfill your needs and goals. At the same, make sure you reciprocate and allow your alliances to leverage you as well....

Stay in the driver's seat, but don't ditch technology

Easy. I am not trying to start some kind of technology for technology's sake argument here. It's very clear that not even the people who are in the business of building and selling technology should head down that path. However, in the last twenty or so years as wave a...

NameProtect roots out fake tsunami-related donation sites

Internat...

Dont rely on information technology to change corporate culture

With electronic productivity and communications tools now ubiquitous, organizations of all types are eager to harness their potential to promote more effective communication and collaboration among workers. While these new capabilities have an important role in organizations, the belief that technology by itself can be used to modify the culture and behavior of an or...

Dont work for free on a maybe someday

You can ply your trade inside top companies for 15 or 20 years and have an outstanding resume and all you have to do is hang out a shingle. All of a sudden, people are c...

Don't be muzzled in your Midwest technology job search

The big thing my current job lacks is career advancement, she told me. So this headhunter sends me on an inter...

How to thwart the dont-be-right Midwest technology boss

Through your career, you've carefully watched and listened and learned so you can excel in your field and do the right thing at the right time. Ha ha! Joke's on you! Sometimes, for some Midwest...

Innovate, Automate, or Improve? You Don't Have to Choose.

Theres an old saw around business computing that you should improve processes before you automate them. It is seductive to think that if the business side could just get its act together and tell us clearly what it wants, everything would be fine. And if your only objective is to automate what youre already doing, it might (underline the might) work. But that leaves a lot of technology value on t...

'Don't work here' job interviews set new Midwest technology trend

I call it the "humanizing factor" at work. A lot of things are still wretchedly out of whack about the corporate world and the behavior of people in it, but in little ways, people are starting to act more and more like regular people at work (sometimes when you least expect it)....

Don't count out notebooks

Notebook computers have become true desktop replacements while fulfilling -- via integrated wireless -- the ultimate requirements for go anywhere, do anything mobile computing. As importantly, the newest notebooks from Hewlett-Packard and Toshiba have broken the traditional price barriers that have made notebooks a luxury alternative to desktop PCs. The <a href="http://www.shopping.hp.co...

Cisco Chairman donates $31 million for UW building renovation

The University of Wisconsin-Madisons Education Building, which originally housed the College of Mechanics and Engineering has been unfinished for 104 years, but thanks to a $31 million alumni gift the university will modernize and complete the building, one of Bascom Hills signature historic landmarks. John P. and Tashia F. Morgridge, both UW-Madison alumni, hope the Education Building c...

GE Healthcare donates $530K to research award program

Waukesha, Wis. -- The goal of the three-year, $530,000 contribution will...

Checking out or getting out? Reasons for shopping cart abandonment

People used to abandon online shopping carts because they didnt understand how online shopping works. Now, it's because they do. Despite ongoing efforts to streamline online purchasing processes, online retailers continue to experience staggering abandonment rates: A recent study by Kefta Inc., a San Francisco-based technology provider, indicates that 50 to 90 percent of all shopping c...

We Need Techo-Savy Leaders - Dont just blame government and education for economic problems

At last years 2002 Wisconsin Economic Summit it was suggested that we focus on expanding the high-tech sector of our economy as the quickest way to create faster economic growth and raise our per capita income above the national average. It was pointed out that our basic industries are in relatively good shape, but we are missing the high-tech, high-growth engine that can pull our economic train...

Wisconsin McDonalds Go Wireless

MADISON, WI - restaurants recently began installing wireless fidelity (Wi-Fi) Internet connections in five Milwaukee locations as part of a six-month pilot program. These Milwaukee locations join select McDonald's restaurants in the San Francisco Bay Area, Me...

Exclusive WTN Interview with Don Williamson

Dan Williamson, president and co-founder of has a powerful new proprietary technology that digests waste. Starting at the far reach...