Detection

Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins

-TaggedFusion Proteins Qiyi Xie Quinn Lu Tanya Hosfield Peter Pingerelli Weiping Yang Stratagene...

A New PCR-based Mycoplasma Detection Method

Stratagene akit that uses a PCR-based method which specifically amplifies an 874-bpPCR product from a single copy gene. In addition, the kit contains an int...

HSVision Molecular Beacon Detection Module Rapidly Detects Herpes Simplex Virus DNA

Single-tube amplification method for high-throughput screening of HSV Cindy Walker-Peach Beti Belachew Peter Pingerelli Melanie Palmer Dwight DuBois Stratagene Stratagene introduces the HSVision molecular beacon detectionmodule,...

Detection and Identification of Phosphorylation Sites in Proteins Using LC/MS/MS with Neutral Fragment Loss Mapping

Michaela Scigelova, Gary Woffendin; Thermo Finnigan, Hemel Hempstead, UK. Malcolm Ward, Helen Byers; Proteome Sciences, London, UK. Diane Hanger; Institute of Psychiatry KCL, London, UK. The data presented here c...

Detection of K-ras Point Mutations in the Pancreas by Constant Denaturing Gel Electrophoresis Using the DCode System

Martin A.O.H. Menke*, Axel Reinecke-Lthge*, Barbara Mllmann*, Angela Kellner, Jutta Lttges*, Gnter Klppel* * Department of Pathology and Department of Hematopathology, Christian- Albrechts University Kiel, Michaelisstrae 11, D-24105 Kiel, Germany. Introduction Genetic alterations/variations are...

Detection of Mutations in the Fas Antigen of Lymphoma Tumors by RT-PCR and Denaturing Gradient Gel Electrophoresis

Terry H. Landowski, Ibrahim Buyuksal, and William S. DaltonH. Lee Moffitt Cancer Center and Research Institute, University of SouthFlorida, 12902 Magnolia Drive, Tampa FL 33612 Introduction Identifying of mutations within specific genes has become an important strategy in determining the diagnosis and prognosis f...

Detection of Mutant K-ras in a Kindred With Hereditary Pancreatic Cancer by DGGE

David A. Crispin,* Ru Chen,* Michael B. Kimmey,** Teresa A. Brentnall** * Departments of Pathology and **Medicine, Division of Gastroenterology, University of Washington, Seattle, Washington. Introduction Pancreatic cancer is the fourth leading cause of cancer death in the U.S....

Detection of Platinum Species in Plant Material by Preparative Isotachophoresis Using the Model 491 Prep Cell, Rev A

Contributed by J. Messerschmidt and F. Alt, Institut frSpektrochemie und angewandte Spektroskopie, Bunsen-Kirchhoff-Str. 11, D-44139 Dortmund, Germany Abstract An isotachophoretic method is presented for the separation of platinum containing proteins from plant material in the Model 491 Prep Cell. Platinum-contai...

Detection of Variation in Highly Polymorphic Mhc Genes by Denaturing Gradient Gel Electrophoresis Using the DCode System

Kristi M. Miller, Tobi J. Ming and Ruth E. Withler, Pacific Biological Station,Department of Fisheries and Oceans, Nanaimo, B.C. V9R 5K6, Canada Introduction We describe a rapid and sensitive method for the detection of nucleotide sequence variation which can be used for largescale screening of population...

Detection of p53 Gene in Breast Cancer by Denaturing Gradient Gel Electrophoresis and the DCode System

Tochigi Cancer Center, Tochigi, Japan Introduction Denaturing gradient gel electrophoresis (DGGE) is one of the most consistently used mutation scanning methods. It has evolved enormously over the last decade to be widely used particularly in gene diagnostic l...

Receptor Binding Assays using Fluorescence Polarization Detection: the [FP]2TM Characterized Tracers

. The process is typically highly automated and can provide screening rates over 100,000 samples in a 24 hour period. To achieve this, assays must be simple, p...

TKXtra DataFile: Homogenous tyrosine kinase detection using High Efficiency Fluorescence Polarization

Uses High Efficiency Fluorescence Polarization (HEFP) detection method Assays a broad range of tyrosine kinases Requires no separation or wash steps Available in a kit and in bulk packaging Screening for tyrosine kinase activity can be accelerated...

cAMP DataFile: Homogenous Cyclic AMP detection using High Efficiency Fluorescence Polarization

A Sensitive, Robust, Homogenous, and Rapid Assay Uses High Efficiency Fluorescence Polarization (HEFP) detection method Detects pmol amounts of cAMP Optimized for cell extract and enzyme preparations Requires no separation or wash steps Available in a kit and in bulk packaging ...

Calcium Plus Assay Kit, Superior Detection for Specialized Targets

HIGHER MAXIMUM SIGNAL RAPID PROCEDURE COMPATIBLE WITH MORE TARGETS SUITABLE FOR HTS APPLICATONS The Calcium Plus Assay Kit provides a reliableand homogeneous method for detecting changesin intracellula...

Detection of mRNAs on Cryosections of the Cardiovascular System Using DIG-Labeled RNA Probes

The following protocol was optimized from a protocolusing 35S-labeled RNA probes [1, 2]. In research studies,it enables detection of the expression of rare mRNAs inthe cardiovascular system (e.g., of the proinflammatorycytokine granulocyte macrophage colony stimulating...

Gene Expression Arrays: Highly Sensitive Detection of Expression Patterns with Improved Tools for Target Amplification

As the human genome project is completed, microarray technology offers the potential to study the genomes complexity. This technology facilitates the direct extraction of functional information from nucleic a...

The DIG System Nonradioactive and Highly Sensitive Detection of Nucleic Acids

Roche Applied Science was one of the first companiesto offer a nonradioactive technology allowing customersto move away from the use of hazardousradioactive isotopes. Even after 15 years and theincreasing availability of many different competitorproducts and quantita...

Quantitative Measurement of Cell Proliferation Using the BrdU ELISA: A Comparison Between Colorimetric and Chemiluminescent Detection

Quantitative determination of DNA synthesis in cellcultures is now a routine procedure in many laboratories.Protocols are available for various applications,especially in cell culture systems. The effects of growthfactors, inhibition of cell division by exogenous factor...

Quantification of Nucleosomes in Serum by the Cell Death Detection ELISAplus

Cell death as a counterpart to cell proliferation plays anessential role in the homeostasis of cell numbers in adultorganisms, furthermore in eliminating cells damaged byirradiation, chemotherapeutic drugs, hyperthermia andother influences [1-3]. There are two distinct...

A Further Step in Understanding Apoptosis Direct Detection of PARP Cleavage

Detection of early apoptotic events has become quiterelevant since many studies have demonstrated thatabnormalities in cell death regulation cause a variety ofdiseases and pathological conditions accompaniedeither by cell accumulation (e.g., cancer) or by cell loss(e...

In Situ Cell Death Detection Kit

The In Situ Cell Death Detection Kit has been designed as a precise, fast, and simple non radioactive technique to detect and quantify apoptotic cell death at the single-cell level in tissues. It is based on the so-called TUNEL method....

Monitoring transfection efficiency by green fluorescent protein (GFP) detection with the Agilent 2100 bioanalyzer

This Application Note describes how the Agilent 2100 bioanalyzer andthe Cell Fluorescence LabChip kit can be used to determine the efficiencyof transfection of mammalian cells using green fluorescent protein(GFP) as a reporter molecule. Transfection of CHO-K1 cells with...

Apoptosis detection by annexin V and active caspase-3 with the Agilent 2100 bioanalyzer

This Application Note describes how the Agilent 2100 bioanalyzer canbe used to analyze apoptotic cell samples. Performance of two assaysfor apoptosis detection is demonstrated and the data are compared tothose obtained with a conventional flow cytometer. Histogram quality...

Detection of antibody-stained cell surface and intracellular protein targets with the Agilent 2100 bioanalyzer

This Application Note describes how the Agilent 2100 bioanalyzer andthe Cell Fluorescence LabChip kit can be used to detect cell surfaceand intracellular protein targets by antibody staining. CD3 staining onJurkat cells as well as CD4 staining on CCRF-CEM cells and intrace...

Sensitive detection of tumor cells in peripheral blood of carcinoma patients by a reverse transcription PCR method

Occurrence of tumor cells in theperipheral blood of individuals sufferingfrom cancer may serve as anearly indication that the primarytumor has dispersed from its tissueof origin. Despite defence mechanismsof the organism, individualcancer cells may attach in di...

Rapid detection of genomic duplications and deletions using the Agilent 2100 bioanalyzer

Duplications and deletions are known to cause a wide variety of humandiseases. Many different methods are used for detecting such rearrangements,most based on either FISH or the quantitative analysis of PCR products.FISH is popular and accurate, but very labour intensive a...

Mutation detection for the K- rasand P16 genes

Mutations in the K-ras gene codon 12 region can lead to cancer, forexample of the colon, pancreas, liver, spleen, stomach or lungs. TheCDKN2A/P16 gene is a familial melanoma gene. Routine PCR and DNAsequencing methods can identify exactly which point mutation is present...

Your Data: Detection of Oocyte-specific Gene Expression Using Ambion's Cells-to-cDNA Kit

Ambion's Cells-to-cDNA Kit produces cDNA ideal for RT-PCR based gene quantitation, including experiments performed using real-time RT-PCR. The Cells-to-cDNA Kit comes in an easy-to-use format that produces first-strand cDNA from cultured cells without RNA isolation in less than 2 hours. By using an RT-compa...

Detection of Genetically Modified Soybean in Processed Foods Using Real-Time Quantitative PCR with SYBR Green I Dye on the DNA Engine Opticon 2 System

MJ Research, Inc., South San Francisco, CA We present a real-time quantitative PCR (qPCR) protocol for the detection and quantitation of soy genetically modified to be resistant to the herbicide glyphosate. The assay utilizes SYBR Green...

Real-Time Multiplex PCR from Genomic DNA Using the iCycler iQ Detection System

Faye Boeckman, Larissa Tan, Marni Brisson, Rob Park, and Keith Hamby,Bio-Rad Laboratories, Inc., 2000 Alfred Nobel Dr., Hercules, CA 94547 When researchers need to measure the amount of RNA or DNA in a preparation, they typically employ a traditional biochemical approach. High concentrations of...

UV Nicking of DNA in Agarose Gels for Enhanced Transfer and Detection of Megabase Size DNAs

The transfer efficiency of large (>100 kb) DNAs from agarose gels to membrane is poor unless the DNAs are nicked prior to transfer. Although this has traditionally been accomplished with HCl depurination, UV irradiation (254 nm) provides a faster and more consistent alternative to HCl nicking....

Detection of Phosphorylated and Total ERK and p38 MAPK by Bio-Plex Assay, ELISA, and Western Blotting, Rev A

Qian Gao, Kris Simonyi, and Claudia Suen, Bio-Rad Laboratories, Inc.,2000 Alfred Nobel Drive, Hercules, CA 94547 USA Western blotting is a traditional technique for detecting phosphorylated proteins within a variety of cell culture and tissue sample lysates. In recent years, new techniques tha...

Simultaneous Detection of 8 Cytokines in Mouse or Human Sera

Aiguo Zhang, Allora Aguilera, Chate Luu, Ivan Huang,Diana Davis, Chloe Etienne, Yong Song, Reni Thomas,Kris Simonyi, Jim Wong, Quan Nguyen Bio-Rad Laboratories, Life Science Group, 2000 Alfred Nobel Drive, Hercules, CA 94547 Presented by the Author at The American Society for Cell Biology...

Identification of Nonspecific Products Using Melt-Curve Analysis on the iCycler iQ Detection System, Rev A

Marni Brisson, Larissa Tan, Rob Park, and Keith Hamby,Bio-Rad Laboratories, Inc., 2000 Alfred Nobel Dr., Hercules, CA 94547 USA Real-time PCR* is a powerful and effective technique for accurate quantitation of DNA. Assays for the detection of a single gene involve careful choice of primers, tar...

Quantitation of Lymphangiogenesis Using the iCycler iQ Real-Time PCR Detection System and Scorpions Detection System, Rev A

Giles Cunnick and Wen Guo Jiang, Metastasis Research Group, UniversityDepartment of Surgery, University of Wales College of Medicine, Cardiff, UK Lymphangiogenesis refers to the formation of new lymphatic vessels. This process may occur in normal developing tissues or in tumors. In addition to sprea...

Rapid, Reproducible Real-Time Quantitative RT-PCR Using the iCycler iQ Real-Time PCR Detection System and iQ Supermix, Rev A

Departments of Ophthalmology and Cell Biology, Duke University Medical Center, Durham, NC 27710 USA Correspondence: Catherine Bowes Rickman, PhD, Department of Ophthalmology, Duke University Medical Center, Box 3802, Durham, NC 27710, Phone (91...

Quantitation of GAD67 Gene Expression in Prefrontal Cortex of Schizophrenia Patients Using the iCycler iQ Detection System and Molecular Beacons, Rev A

130 West Kingsbridge Road, Bronx, NY 10468 USA Schizophrenia is a severe mental illness that afflicts about 1% of the population (Strange 1992). Patients experience a variety of symptoms that have been divided into subgroups...

Real-Time PCR/Melt-Curve Analysis: SNP Detection With FRET, Rev A

Bio-Rad Laboratories, 2000 Alfred Nobel Drive, Hercules, CA 94547 USA The detection of fluorescence resonance energy transfer (FRET) through real-time polymerase chain reaction (PCR) and melt-curve analysis is a powerful...

The iCycler iQ Detection System for TaqMan Assays, Rev B

Faye Boeckman, Keith Hamby, and Larissa Tan, Bio-Rad Laboratories, 2000 Alfred Nobel Drive, Hercules, CA 94547 USA Specific fluorescent oligonucleotide probes allow for real-time monitoring of the polymerase chain reaction (PCR*). One popular probe strategy is the TaqMan assay...

The iCycler iQ and Amplifluor Detection Systems for Analysis of Genetically Modified Organisms in Foodstuffs, Rev A

Andrew Tingey, Reading Scientific Services Ltd., The Lord ZuckermanResearch Centre, Whiteknights, PO Box 234, Reading RG6 6LA, UK One of the most topical issues in the food industry is the use of genetically modified organisms (GMOs) in agriculture and food production. Demands from consumers, particu...