DNA
Navigation Links
DNA at biology technology
New Microarray Labeling Kit for Preparing cDNA Probes
A more reliable and reproducible way to create fluorescently orradioactively labeled cDNA David T. Wong Heidi Davis Darryl Garrison TerryPayette Matt Lundy Kim Kuplent Mary A. Buchanan Joseph A. Sorge Rebecca L. Mullinax Stratagene Phenogenex Nucleic acid micro...Purified Single-Stranded M13 DNA for Automated Sequencing
A streamlined and reliable method to obtain high-quality single-strandedDNA Stratagene M13 DNA purification kit allowseasy, quick, and reliable purification of bacteriophage single-stranded DNA. Theyield of single-stranded DNA recovered from phage lysa...Low Abundance cDNA Cloned Using Stratagenes Human Universal cDNA Library
Department of Cell Biology, Clevela...Fast and Easy Isolation of PCR-Ready Genomic DNA from Whole Blood
...Novel PCR Enhancing Factor Improves Performance of Pfu DNA Polymerase
pfuturbo DNA polymerase for robust, high-fidelity PCRamplification Holly Hogrefe Brad Scott Kirk Nielson ValerieHedden Connie Hansen Janice Cline Frances Bai Jeff Amberg RondaAllen Mark Madden Stratagene an improved version of Pf...Human Universal cDNA Library Array I
Jean-Michel Llias Leonardo de Leon Bruce Jerpseth Sandra Soares Stratagene Stratagene has organized over 145,000 high-quality clones from a universal cDNA libra...Pfu DNA Polymerase as a Hot Start Alternative
Stratagene Cloning Systems, Inc. This article describes the important, novel application of Stratagenes PfuDNA polymerase for minimizing PCR cold start mismatched primer-extensionreactions. These mismatched extension rea...PCR Performance Comparisons Between pfuturbo and Taq DNA Polymerases
pfuturbo DNA polymerase is the superior choice for robusthigh-fidelity PCR amplification Stratagene In this update, we compare the PCR performances of pfuturbo DNApolymerase * ,</...pfuturbo DNA Polymerase: A High-Performance, High-Fidelity Enzyme Ideal for PCR Cloning
A High-Performance, High-Fidelity Enzyme Ideal for PCR Cloning Stratagene was cloned into the PC...New Protocols for Isolating High- Molecular-Weight Genomic DNA
Stratagene Stratagene now offers updated protocols to isolate and purifyhigh-molecular-weight genomic DNA using the RecoverEase DNAisolation kit. New procedures include DNA isolation from blood, bo...A Simple and Rapid Technique to Purify High-Quality Midiprep DNA
Stratagene A new technique is now available to quickly, easily, and reliably isolatehigh-purity plasmid DNA. The convenient spin-format eliminates the need forresin suspensions, gravity flow columns, and alcohol precipit...Functional Cloning Using ViraPort Retroviral cDNA Expression Libraries
Retroviral libraries with high-titer efficiency and larger insertpotential ViraPort Retroviral cDNA Expression Libraries Katherine Felts Kim Zaharee Latha Sundar JamieLimjoco Anna Waesche Peter Vaillancourt Stratagene We introduce a set of human cDNA expression li...Simple Purification of DNA from Plasmid Minipreps, PCR Amplifications and Agarose Gels
High yields of extremely pure DNA with StrataPrep nucleic acidpurification kits Stratagene Cloning Systems, Inc. Stratagene announces a new line of DNA purification products, whichprovide reliable isolation of high-purity miniprep plasmid, PCR product andgel-fractionate...CastAway Precast Gels for Rapid Automated DNA Sequence Analysis
Stephen Roemer Mary Jensen Harry Osterman MarkVanek LI-COR Biotechnology Division LI-COR and Stratagene release the first commercially available precastgels for automated DNA sequencing and genetic analysis. Stratagenes CastAway gels* for...Easily Amplify Genomic DNA with Long-Distance PCR
Long DNA polymerase to genotypemice Cardiovascular Research Center, University of Cincinnati, Cincinnati, OH We developed a simple, reliable procedure to genotype mice usinglong-distance PCR, whereby genomic DNA is isolated from mouse ear clips, an...Fractionating DNA Fragments Generated by Differential Display PCR
Pfizer Central Research, Sandwich, UK. 01304 618482 Differential display PCR (DDPCR) is a widely used technique foridentifying genes differentially expressed between cell types. Application ofDDPCR to allergic disease provides a potential means for elucidating themechanisms involved in allergic...Constructing Directional cDNA Libraries from Limited Amounts of RNA
Barry R. Neiditch Cherie Dewar Tanya Hosfield Marie Callahan John C. Bauer Mike Kobrin Quinn Lu Dianne Eckhardt Stratagene Human Genome Sciences, Rockville, Maryland Stratagene has developed the PCR Library Con...Generate High-Quality, Directional Plasmid cDNA Libraries
Produce larger primary libraries with new plasmid library constructionkits Quinn Lu Marie Callahan Tanya Hosfield BarryNeiditch Alan Greener John C. Bauer Mike Kobrin Stratagene II,pcmv-script and pAD-GAL4-2.1 XR library construction kit...Unique DNA Polymerase Formulation Excels in a Broad Range of PCR Applications
Herculase enhanced DNA polymerase delivers high fidelity and greatperformance Stratagene Stratagene has recently released Herculaseenhanced DNApolymerase,* a novel enzyme formulation that providessuperior performance in both routine and demanding PCR applications. Compariso...Enhanced Amplification of Long Targets with PfuTurbo DNA Polymerase
DNAPolymerase Stratagene DNA polymerase* by making simple modifications to PCR reactionconditions. In this update, we d...HSVision Molecular Beacon Detection Module Rapidly Detects Herpes Simplex Virus DNA
Single-tube amplification method for high-throughput screening of HSV Cindy Walker-Peach Beti Belachew Peter Pingerelli Melanie Palmer Dwight DuBois Stratagene Stratagene introduces the HSVision molecular beacon detectionmodule,...Optimizing pfuturbo DNA Polymerase Amplification Reactions with Perfect Match PCR Enhancer
Optimizing pfuturbo DNA Polymerase Amplification Reactions withPerfect Match Stratagene Certain PCR amplification systems produce multiple pro...Unique Enhanced DNA Polymerase Delivers High Fidelity and Great PCR Performance
Improve reliability of PCR reactions with Herculase enhanced DNApolymerase Stratagene enhanced DNApolymerase,* a novel enzyme formulation that providessuperior performance in both routine and demanding PCR applications. Previous...Easy Cosmid DNA Purification with StrataPrep Kits
Stratagene plasmid miniprep and midiprep kits aredesigned to isolate up to 20 and 350 g, respectively, of high-purity plasmidDNA from a single spin cup. These kits can also easily purify verylarge plasmids, inc...Sequence-Validated and Expression-Tested Human cDNA in a Dual Expression Vector
Study protein function more efficiently with standardized clones Rebecca L. Mullinax Heidi Davis David T. Wong Kelly Wynne Vilma Nioko Leonardo DeLeon SanDEe Soares Joseph A. Sorge Edward Marsh Stratagene Phenogenex Stratagene now offers s...Stratagenes Human cDNA Microarrays: Perform Gene Expression Profiling and Discover New Genes
New GeneConnection discovery microarrays provide more than 4,000 uniquehuman cDNA sequences Stratagene introduces the first in a series of human cDNA microarrays on glass slides. These GeneConnection discovery microarrays which are based on the technology published by Schena et al., can be...Restriction Digests of DNA Isolated with the Perfectprep Gel Cleanup Kit
Gel Cleanup Kit George Halley, Eppendorf 5 Prime, Inc. Agarose gel electrophoresis is used extensively to separate and analyze DNA that is subsequently used for downstream applic...Lambda DNA Extraction Protocol
This method produces good quality LambdaDNA with yields of 1520 g lgt10 DNA per 450 l of supernatant processed. PLG tubes also can be used in conjunction with other LambdaDNA purification methods. (1) Grow a 10 ml stock of suitable plating bacteria in L-broth plus Mg2...M13/Phagemid DNA Extraction Protocol
Efficiency of the standard protocols for production of single-stranded DNA may be improved through the use of PLG. For producing M13 ssDNA, mix 2.5 ml plating bacteria (57...Genomic DNA Extraction from Buccal Swabs Using the Perfect gDNA Blood Mini Kit
Eppendorf 5 Prime, Inc. Buccal swabs are the least invasive way of collecting gDNA from humans, and also have the added benefit of minimizing the exposure to blood-borne pathogens. Buccal sw...Genomic DNA Extraction from Buffy Coat Using the Perfect gDNA Blood Mini Kit
Eppendorf 5 Prime, Inc. The Perfect gDNA Blood Mini Kit is an efficient way to purify genomic DNA from 200 l of whole blood as described in the product manual. In addition,...EppendorfPerfect gDNA Blood Mini Kit
Isolation of Coxiella Burnetii Genomic DNA from Goat Placenta, Mouse Spleen, and Bovine MilkUsing the Perfect gDNA Blood Mini Kit INRA centre of TOURS, Unit de Pathologie Infectieuse et Immunologie, 37380 NOUZILLY FRANCE Phone: (33)...Higher gDNA Concentrations with the Perfect gDNA Blood Mini Kit by Varying the Final Elution Volume
by Varying the Final Elution Volume Eppendorf 5 Prime, Boulder, Colorado Perfect gDNA Blood Mini Kit protocol recommend...Isolation of Genomic DNA from Saliva Using the Perfect gDNA Blood Mini Kit
Eppendorf 5 Prime, Inc. It is not convenient in all instances to have blood drawn for gDNA extraction. The Eppendorf Perfect gDNA Blood Mini Kit was used to extract gDNA from...Mouse Tail Genomic DNA Isolation Protocol(1)
Note: Genomic DNA is fragile. High molecular weight DNA is sheared easily by mechanical forces. Use suitable large-bore pipet tips or equipment when pipetting genomic DNA. Do not vortex solutions containing genomic DNA. Place a 1 cm tail sample into a 1.5 ml microcentrifuge tube; this may be stored...Genomic DNA Isolation Protocol(1,2,3)
Note: Genomic DNA is fragile. High molecular weight DNA is sheared easily by mechanical forces. Use suitable large-bore pipet tips or equipment when pipetting genomic DNA. Do not vortex solutions containing genomic DNA. Caution: Wear suitable...Basic Plasmid DNA Isolation Protocol
PLG can be used in small and large scale protocols based on alkaline lysis procedure.(1,2,3) The basic protocol given here works with E. coli cultures grown for 1214 hours in up to 500 ml of amplified or non-amplified LB or DYT, or in 250 ml Terrific Broth....RNA-free Plasmid DNA Isolation Protocol
The following protocol is for use with 1 liter cultures grown in LB medium for 1214 hours. It is also used for cultures grown for no longer than 1214 hours in 0.5 liter of enriched growth media such as Terrific Broth (TB). The reagents used in the cleared lysate preparation (step 15) should be scaled...Lambda DNA Extraction Protocol
This method produces good quality LambdaDNA with yields of 1520 g lgt10 DNA per 450 l of supernatant processed. PLG tubes also can be used in conjunction with other LambdaDNA purification methods. (1) Grow a 10 ml stock of suitable plating bacteria in L-broth plus Mg2...Genomic DNA Extraction from Buccal Swabs Using the Perfect gDNA Blood Mini Kit
Eppendorf 5 Prime, Inc. Buccal swabs are the least invasive way of collecting gDNA from humans, and also have the added benefit of minimizing the exposure to blood-borne pathogens. Buccal sw...Other TagshomologAlpcoEIAEIARSHRSHRSHRSHRSHOMeOMeOMeOMeOMeOMeOMeOMeOMeHannifinParkerBioReagentsABRABRABRABROther Contentshemorrhoidhemorrhoidshemostasishemostasisencephalopathyencephalopathyencephalopathyhepatichepatichepatichepatichepatichepatomegalyhepatomegaly
(Date:7/24/2008)... Pennsylvania State University College of Medicine...fficacy of imiquimod, a clinically important immun...tumor activity, is dependent on the Opioid Growth ...on. This discovery, reported in the August 08 iss...s new insights into a widely used drug that may le...
(Date:7/24/2008)...UC Irvine researchers have found a molecular link ... metabolism. The discovery reveals new possibiliti...r related diseases. , Paolo Sassone-Corsi, Disti...his colleagues have identified that an essential p...adian rhythms, works in balance with another prote...
(Date:7/24/2008)...el Structure Proteins (NSP), a new family of genes... for Cancer Research and Molecular Medicine in Tem..., could be involved in apoptosis or programmed cel...by Nianli Sang, then a doctoral student at the Uni...efferson University and the Cardeza Foundation, an...
(Date:7/24/2008)...A single typhoon in Taiwan buries as much carbon i...he other rains in that country all year long combi...ersity study published in a recent issue of the jo...o examine the chemistry of stream water and sedime...oon was happening at full force -- will help scien...
Breaking Biology News(10 mins):Imiquimod, an immune response modifier, is dependent on the OGF-OGFr signaling pathway 2Circadian rhythm-metabolism link discovered 2Novel structure proteins could play a role in apoptosis 2Study: Typhoons bury tons of carbon in the oceans 2Study: Typhoons bury tons of carbon in the oceans 3Study: Typhoons bury tons of carbon in the oceans 4Rheumatoid Arthritis Drugs May Lower Heart Attack Stroke Risk 13725 1Rheumatoid Arthritis Drugs May Lower Heart Attack Stroke Risk 13725 2Function of molecular switch pinpointed in severe congenital neutropenia 13723 1Function of molecular switch pinpointed in severe congenital neutropenia 13723 2Function of molecular switch pinpointed in severe congenital neutropenia 13723 3Empire Blue Cross Blue Shield Statement in Response to New York Attorney Generals Recent Subpoenas 13721 1Jazz Pharmaceuticals Inc Announces Webcast of March 13 Investor Meeting 3772 1(Date:7/25/2008)...ON, July 25 /PRNewswire/ -- The Elekta Family of C... treatment solutions at the 2008,American Associat...g,demonstrating how the joint forces of Elekta, I... therapy planning and delivery solutions that,stre...perience for,patients, as well as enhance clinicia...
(Date:7/25/2008)...ds fluid back-up in esophagus can cause immune sys...ay News) -- The first evidence linking gastroesoph...scovered by Duke University Medical Center researc...ns was first noted in the 1970s, and since then st...rcent of asthma patients also experience GERD symp...
(Date:7/25/2008)... The Retina Group of New York will...ted macular degeneration especially designed for s...e Public Library at 116 Merritts Road, Farmingdale.... Attendance is free and refreshments will be prov...6) 939-6100. , Hicksville...
(Date:7/25/2008)... This donation will be used to fac...ve services for children , ... ( www.ivci.com ), a leading integrator of enterp...tion video conferencing, telepresence, audio visua...announced today that it has donated video conferen...
Breaking Medicine News(10 mins):Health News:Elekta Family of Companies to Highlight Cancer Treatment Solutions at 2008 AAPM Annual Meeting 2Health News:Elekta Family of Companies to Highlight Cancer Treatment Solutions at 2008 AAPM Annual Meeting 3Health News:People With GERD More Likely to Develop Asthma 2Health News:Retina Group of New York To Present Seminar on Age-Related Macular Degeneration; The Leading Cause of Visual Loss in Seniors 2Health News:Retina Group of New York To Present Seminar on Age-Related Macular Degeneration; The Leading Cause of Visual Loss in Seniors 3Health News:Mt. Sinai's Children's Trauma Institute Treatment and Service Adaptation Center Receives Video Conferencing Donation From IVCi 2[0] Elekta Family of Companies to Highlight Cancer Treatment Solutions at 2008 AAPM Annual Meeting[0] People With GERD More Likely to Develop Asthma[0] Retina Group of New York To Present Seminar on Age-Related Macular Degeneration; The Leading Cause of Visual Loss in Seniors [0] Mt. Sinai's Children's Trauma Institute Treatment and Service Adaptation Center Receives Video Conferencing Donation From IVCi
| Copyright © 2003-2008 Bio-Medicine. All rights reserved. ABOUT | CONTACT US | DISCLAIMER | PRIVACY POLICY | TERMS AND CONDITIONS |  |