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Automated Fluorescent-Tag Cycle Sequencing

Stratagene Cloning Systems, Inc. *,# with Hot Top Assembly can be used to perform fluorescent-tag cyclesequencing using the ABI PRISM dye terminator cycle sequencingr...

Effects of Thermal Cycler Well-To-Well Temperature Uniformity on PCR Results

Michelle Cayouette Jane Moores Jason Capodanno Michael Rahilly Stratagene produces consistent PCR results in every well. The RoboCycler systems uniquedesign uses thermal blo...

New Temperature Cycler Features Interchangeable Blocks

Michelle Cayouette Jane Moores Jason Capodanno Michael Steege Michael Rahilly Stratagene Infinitytemperature cycler, a new temperature cycler withinterchangeable blocks for...

Optimizing the Direct Amplification of Missing cDNA 5' Ends Using the Eppendorf Mastercycler gradient

Using the Eppendorf Mastercycler gradient Institute of General Botany, University of Hamburg Christian Rohrer, Eppendorf AG, Hamburg In man...

Optimizing DNA Amplification Protocols using the Eppendorf Mastercycler

Peter Scheinert, PhD, Barbara Behrens, PhD, and Dietmar Kahle, PhD Peter Scheinert, PhD, Bernhard-Nocht-Institute for Tropical Medicine, Virology Dept., Bernhard-Nocht-Str. 74, D-20359 Hamburg former address: University of Kiel, Institute for General Microbiology, Am Botanischen Garten, D-24118 K...

Amplification of Epstein-Barr Virus Exon C with Difficult Primers Using Eppendorf Mastercycler gradient

Using Eppendorf Mastercycler gradient NPC Laboratory, Institute of Postgraduate Studies and Research, University of Malaya, Kuala Lumpur, Malaysia...

The use of the Mastercycler gradient for single-cell PCR and preimplantation diagnosis

Karen Sermon, M.D., PhD Center for Medical Genetics, University Hospital and Medical School of the Dutch-speaking Brussels Free University, Laarbeeklaan 101, 1090 Brussels, Belgium Preimplantation diagnosi...

Optimizing the Direct Amplification of Missing cDNA 5' Ends Using the Eppendorf Mastercycler gradient

Using the Eppendorf Mastercycler gradient Institute of General Botany, University of Hamburg Christian Rohrer, Eppendorf AG, Hamburg In many research projects, cD...

Eppendorf Mastercycler ABI Big Dye Sequencing Protocol

From MAIN MENU go to FILES/ EDIT/ STANDARD rewrite accordingly then EXIT/ YES (use SEL to choose)/ ENTER/ change name to BIGDYE/ ENTER...

Mastercycler Gradient Quick Programming Guide

USE ARROW KEYS TO SCROLL THROUGH MENUS AND PROGRAMS. PRESS ENTER TO SELECT HIGHLIGHTED CHOICE) Menu items are chosen by first positioning cursor to the choice desired, then press . This confirms choice or input data or changes display to next menu in hierarchy....

The LightCycler is the only System for Quantitative PCR that is as Flexible as You are!

The LightCycler is the only System for Quantitative PCR that is as Flexible as You are! Choose the LightCycler PCR System, the only instrument flexible enough to meet your changing research needs. Central to the LightCycler System is the LightCycler Instrument which is much more than "Just an...

cAMP DataFile: Homogenous Cyclic AMP detection using High Efficiency Fluorescence Polarization

A Sensitive, Robust, Homogenous, and Rapid Assay Uses High Efficiency Fluorescence Polarization (HEFP) detection method Detects pmol amounts of cAMP Optimized for cell extract and enzyme preparations Requires no separation or wash steps Available in a kit and in bulk packaging ...

Biotech industry reaches midpoint in 50-year maturation cycle

An interesting article in ) on March 1 states unequivocally that most industry cycles run about 50 years until they mature. If this...

UW-Madison step in ALS research; student business plans; tech recycling

UW-Madison researchers may be moving toward a treatment for ALS, a nerve-system disease, using stem cells. Also, students excel at the G. Steven Burrill Technology Business Plan Contest, and Madison offers a computer and television recycling program....

Thermal Cycling Profile for Standard PCR

It is very important to denature the template DNA completely. Initial heating of the PCR mixture for 2 minutes at 9495C is enough to completely denature complex genomic DNA so that the primers can anneal to the template as the reaction mix is cooled. If the template DNA is only par...

Screening of Beer-Spoilage Bacteria Using the LightCycler PCR Workflow System

The new LightCycler foodproof Beer Screening Kit (dedicatedto the detection of beer-spoilage bacteria on theLightCycler Instrument) in combination with the MagNAPure LC DNA III Kit (for the automated isolation of bacterialDNA on the MagNA Pure LC Instrument) offersbr...

LightCycler foodproof Beer Screening Kit

Breweries make continuous efforts to ensure the highestquality of their goods. To guarantee the consistency ofproduct quality, the different stages of beer production...

Quantification of siRNA Silencing Efficiency Using the LightCycler System

Since the first description of RNA interference (RNAi) asa protection mechanism against invasion of foreign genesappeared a few years ago, RNAi has become a powerfultool for analyzing gene function in mammalian cells. RNAiis mediated by short double-stranded RNAs that a...

Gene-Expression Analysis of TP, DPD, and TS Using the LightCycler mRNA Quantification KitsPLUS

In human cells, the enzymes thymidin phosphorylase(TP), dihydropyrimidine dehydrogenase (DPD), and thymidylatesynthase (TS) play a pivotal role in the metabolismof pyrimidines. In cancer disease medical care, thecytotoxic compound 5-fluorouracil (5-FU) is the standard...

Real-Time Multiplex PCR from Genomic DNA Using the iCycler iQ Detection System

Faye Boeckman, Larissa Tan, Marni Brisson, Rob Park, and Keith Hamby,Bio-Rad Laboratories, Inc., 2000 Alfred Nobel Dr., Hercules, CA 94547 When researchers need to measure the amount of RNA or DNA in a preparation, they typically employ a traditional biochemical approach. High concentrations of...

Identification of Nonspecific Products Using Melt-Curve Analysis on the iCycler iQ Detection System, Rev A

Marni Brisson, Larissa Tan, Rob Park, and Keith Hamby,Bio-Rad Laboratories, Inc., 2000 Alfred Nobel Dr., Hercules, CA 94547 USA Real-time PCR* is a powerful and effective technique for accurate quantitation of DNA. Assays for the detection of a single gene involve careful choice of primers, tar...

Quantitation of Lymphangiogenesis Using the iCycler iQ Real-Time PCR Detection System and Scorpions Detection System, Rev A

Giles Cunnick and Wen Guo Jiang, Metastasis Research Group, UniversityDepartment of Surgery, University of Wales College of Medicine, Cardiff, UK Lymphangiogenesis refers to the formation of new lymphatic vessels. This process may occur in normal developing tissues or in tumors. In addition to sprea...

Rapid, Reproducible Real-Time Quantitative RT-PCR Using the iCycler iQ Real-Time PCR Detection System and iQ Supermix, Rev A

Departments of Ophthalmology and Cell Biology, Duke University Medical Center, Durham, NC 27710 USA Correspondence: Catherine Bowes Rickman, PhD, Department of Ophthalmology, Duke University Medical Center, Box 3802, Durham, NC 27710, Phone (91...

Quantitation of GAD67 Gene Expression in Prefrontal Cortex of Schizophrenia Patients Using the iCycler iQ Detection System and Molecular Beacons, Rev A

130 West Kingsbridge Road, Bronx, NY 10468 USA Schizophrenia is a severe mental illness that afflicts about 1% of the population (Strange 1992). Patients experience a variety of symptoms that have been divided into subgroups...

Real-Time Immuno-PCR on the iCycler iQ System, Rev A

Kristina Lind and Mikael Kubista, TATAA Biocenter, Medicinaregatan 7A/B,405 30 Gteborg, Sweden A technique for antigen detection, called immuno-PCR, was developed by Sano et al. (1992). It combines the molecular recognition of antibodies with the high DNA amplification capability of PCR. The procedu...

The iCycler iQ Detection System for TaqMan Assays, Rev B

Faye Boeckman, Keith Hamby, and Larissa Tan, Bio-Rad Laboratories, 2000 Alfred Nobel Drive, Hercules, CA 94547 USA Specific fluorescent oligonucleotide probes allow for real-time monitoring of the polymerase chain reaction (PCR*). One popular probe strategy is the TaqMan assay...

The iCycler iQ and Amplifluor Detection Systems for Analysis of Genetically Modified Organisms in Foodstuffs, Rev A

Andrew Tingey, Reading Scientific Services Ltd., The Lord ZuckermanResearch Centre, Whiteknights, PO Box 234, Reading RG6 6LA, UK One of the most topical issues in the food industry is the use of genetically modified organisms (GMOs) in agriculture and food production. Demands from consumers, particu...

siRNA screening of the cell cycle with two dynamic GFP sensors (from Discovery Matters Issue 1, July 2005)

M. Kenrick, S. Hancock, S. Stubbs, and N. Thomas GE Healthcare, The Maynard Centre, Cardiff, UK Recent advances in siRNA methodologies and the development of high-throughput image analysis platforms such as the IN Cell Analyzer have revolutionized the functional analysis of genes and proteins. Here, we describe the application of...

Performance of Ad-A-Gene Cyclic AMP Response Element-Nitroreductase (CRE-NTR): an adenoviral vector gene delivery system

adenoviral vector ● viral transduction ● NTR ● IN Cell Analyzer ● gene delivery Biological assays developed in cultured and primary cells can greatly aid secondary screening as well as lead and target validation, but their development presents several challenges. Establishing stable cell systems to expre...

Development of a sensitive enzyme fragment complementation assay for cyclic adenosine 3', 5' monophosphate and its validation for pharmacological screening at G protein-coupled receptors

G protein-coupled receptors (GPCRs) are the largest family of transmembrane receptors and are responsible for the transduction of a diverse range of extracellular signals. Up to 40% of clinically ma...

The Analysis of Polycyclic Aromatic Hydrocarbons (PAHs) by LC/MS/MS Using an Atmospheric Pressure Photoionization Source

The atmospheric pressure photoionization source, PhotoSpray ion source (Robb, Covey and Bruins1) uses photons to ionize large quantities of a dopant molecule added along with the vaporized mobile phase. Analyte molecules are efficiently ionized through secondary reactions initiated by the charged dopant. This new tec...

Quantitative Analysis of the Cell Cycle in Imaging Applications

Quantitative analysis of stages in the cell cycle is commonly used in cell biology since many pharmacological interventions aim to modulate the cell cycle in some way. We present here an application that can be used in adherent cells and optionally multiplexed for the use in automated cell signaling analysis and drug-screen...

Cyclodextrin enhances room temperature phosphorescence

Cyclodextrin-enhanced room-temperature phosphorescence (CD-RTP) is an analytical technique that offers well-resolved spectra and subpicogram detection limits. When polynuclear aromatic hydrocarbons (PAHs) are included in cyclodextrin molecules in the presence of a heavy-atom moiety such as 1,2-dibromoethane, they exhibit intense, well-defined...

QIAGEN Fast Cycling PCR Kit

The QIAGEN Fast Cycling PCR Kit provides the unrivaled performance of HotStarTaq Plus DNA Polymerase with a significantly shortened PCR cycling time on any thermal cycler. The QIAGEN Fast Cycling PCR Kit provides: Ultrafast hot-start PC...

Motorcycling entrepreneur to speak in Madison on success, failure, capital

Hanlon will visit the <a href="http://www....

Technology cycles from advantage to necessity to disadvantage

There is a continual cycle for o...

Computer recycling: An environmental mandate the private sector can support

But what does your home look like? Are your closets or basements cluttered with...

Pacific Cycle rides full speed into RFID

The company, which owns such traditional brands as Schwin...

Is venture capital dead? No, but its beginning a new cycle

Howard Anderson, who teaches courses on early-stage companies at the Massachusetts Institute of Technology, founded or co-founded three venture capital funds. He was a self-described financial samurai. Now, hes hanging up his sword, declining to raise new money for his funds. Theres too much venture money pursuing too few deals. Theres nowhere for all that money to go: we cant spend the...

Graduating class includes biotech networkers; cell-phone recycling

Congratulations to this year's graduating class including 20 students in the Master of Science in Biotechnology program. They worked nights and weekends in this two-year professional program to build up diverse business skills. Also: need something to do with your old cell phone? Recycle it with the UW-Madison police department. <a href="/me...