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Generate Adenovirus Vectors in E. coli by Homologous Recombination with the AdEasy Adenoviral Vector System

Efficient gene transfer and high-level protein expression within a varietyof cells Stratagene Recombinant adenoviruses provide a versatile means of gene delivery andexpression. We describe the AdEasy adenoviral vectorsystem, whichsimp...

Expression and Purification of Recombinant Proteins That Have Native Amino Acid Sequence

Denise L. Wyborski * John C. Bauer * Barbara McGowan * JosephA. Sorge * Peter Vaillancourt Stratagene Cloning Systems, Inc. Stratagene has improved the Affinity protein expression andpurification system by the addition of the pCAL-n-EK vector. This E. coliclonin...

Purification of Recombinant Flavonone 3beta-Hydroxylase Using Bio-Scale CHT5-1 Hydroxyapatite Column

Flavanone 3b-hydroxylase (FHT), a typical 2-oxoglutaratedependent dioxygenase, catalyzes the 3b-hydroxylation of 2S-flavanones to 2R,3R-dihydroflavonols which are intermediates in the biosynthesis of flavanoids, catechins, proanthocyanidins, and anthocyanidins in plants. To understand the complex reaction mechanism,...

Luciferase Reporter Gene Cells and Recombinant Luciferase Dilutions Studied with the CLIPR Luciferase Assay Kit and the LMax Microplate Luminometer (MaxLine Application Note #38)

Evelyn McGown, Ph.D. , Michael Su, M.S. and Jin Liao, M.D., Ph.D. Molecular Devices Corporation, 8/00 Luciferase from the North American firefly (Photinus pyralis) is very popular foruse in Reporter Gene Assays. The enzyme catalyzes the ATP-dependent oxidationof luciferin with the concomitant release of light...

Proteinase K, Recombinant, PCR Grade: the Ideal Tool for Template Preparations

The endopeptidase originally isolated from the filtrate ofthe lower fungus Tritirachium album limber is recombinantlyexpressed in Pichia pastoris. Proteinase K isextremely effective on native proteins from differentsources, and remains active in the presence of denaturingreag...

Combine High Yield, Great Accuracy and the Prevention of Carry-over Contamination by Using the Novel Expand High FidelityPLUS PCR System

Roche Applied Science pioneered the blending of thermostableDNA polymerases and the associated buffersystems. The well-known Expand High Fidelity andExpand Long Template PCR Systems were the first commerciallyavailable blends to provide amplification offragments up t...

Combat RNase Contamination in the Lab

Trace amounts of ribonuclease (RNase) contamination can sabotage experiments. Since RNases are ubiquitous in the laboratory environment--on your skin, in the air, on anything touched by bare hands or on anything left open to the air--it is important to make an effort to prevent...

Recombinant HIV Reverse Transcriptase

Ideal for reverse transcribing transcripts with high G/C content...

Preparative SDS-PAGE Electrophoresis of a Recombinant Epstein-Barr Virus Encoded Protein and Its Application in Serodiagnostic Test Systems, Rev C

Udo Reischl and Christian Gerdes, Institute of Medical Microbiology and Hygiene,University of Regensburg, 93053 Regensburg, Germany Abstract Serodiagnosis of Epstein-Barr virus (EBV) infection is currently based on the detection of antibodies to distinct EBV antigens. In the past few years the specificity...

The combination of monolithic columns and modern HPLC/MS/MS instrumentation for high chromatographic throughput and sensitive detection of beta-agonists

Beta-agonists are group A substances of the European guideline 93/257/EWG (revision 98/536/EG) having an anabolic effect and not licensed. Thus, they have to be determined in veterinarian samples like muscle, liver, retina, and urine. The coupling of High Pressure Liquid Chromatography (HPLC) and Electrospray Ionization Tandem Mass Spectrometry (ESI-MS-MS) seems to be the analytical m...

Cell lines expressing recombinant aequorin and a G-protein coupled receptor for functional screening

Many G-protein coupled receptors (GPCRs) trigger, upon binding of an agonist, a transient increase in intracellular calcium concentration. This variation acts as an internal secondary messenger and is an important modulator of many physiological mechanisms (reviewed by Rink (1990), Tsunoda (1993) and by Santella & Carafoli (1997)). Measure...

While schools combat low tech enrollment, are businesses contributing to IT workforce woes?

Judging by the growing number of technology activ...

Fusion 2007: In e-legalities, CIOs and lawyers combine to provide business value

That was the message of attorney Erik Phelps, a partner in the law firm <a href="http://www.mbfl...

Marshfield Clinic and UW combine to speed tech growth

Naming No. 1 is relatively easy: the ($764 million) is perennially among the top academic R&D centers in the United States. Second on the list is the <a href="http:...

Thompson's HHS legacy will include efforts to combat disease in Africa

Even if former Wisconsin Gov. Tommy Thompson skips a second term i...

Mirus receives $1.26 million SBIR grant to combat anemia

, a gene therapy researcher, announced on Wednesday that it will receive a $1.26 million Phase II Small Business Innovation Research grant from the National Institutes...

NameProtect and MasterCard unite to combat online fraud, phishing

MADISON, Wis. Under the arrangement, NameProtect...

GreetingsPlus combines cards, CDs to form multimedia greeting cards

Expanding on its CD label printing business in Hartland, Wis., The CDs are screen-printed to match the designs on the accompanying cards and will a...

Alfalight Packages 20-Watt Combined Power Module

Madison, Wis. Alfalight, Inc. announced the availability of a 20-watt combined power module (CPM-20) today, a multimode output beam that combines seven high-power emitter outputs. The new combination provides an optical power source designed to be used in industrial, marking, defense, telecommunications and medical laser applications. In combination with available specialty optical fibe...