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Clone in Biological Technology

Caliper Life Sciences Introduces Staccato Protein Workstation for Automated Protein Sample Preparation & Analysis

...sed by increased sample requirements of QbD approaches, and is also directly applicable to research groups performing protein expression optimization, clone selection, and other high throughput techniques," said Kevin Keras, Business Unit Manager, ACES, Caliper Life Sciences. "Caliper has worked with sever...

GeneCopoeia Introduces Genome-Wide MicroRNA (miRNA) Functional Analysis Tools

...pression clones; and 3) All-in-One(TM) miRNA RT-qPCR system. Constructed in a lentiviral vector system, miExpress(TM) precursor miRNA expression clone collections cover all known miRNAs for human (750), mouse (450), and rat (270). These lentiviral vector based expression constructs allow efficient t...

Avid Bioservices Presents Data From Manufacturing Successes Achieved With Single-Use Bioreactor Systems

...has led us to innovate in a number of areas, including installing our two HyClone S.U.B. systems and acquiring a ClonePix automated system for optimal clone selection. These new technologies have been very successful in reducing our processing costs and shortening project timelines." The Thermo Scie...

OriGene Technologies, Inc. Establishes High Throughput Monoclonal Antibody Production Outsourcing Center in China

...o support academic, pharmaceutical and biotech companies for research and drug discovery. Our flagship products are the world's largest cDNA and shRNA clone collections. Other key products include over 100,000 highly validated human tissues through its wholly owned subsidiary Cytomyx LLC., and its tissue d...

J. Craig Venter Institute Researchers Publish Significant Advance in Genome Assembly Technology

... in the end was a tedious, multi-stage process in which the team had to build the genome a quarter at a time using the bacterium Escherichia coli to clone and produce the DNA segments. During this building process the team found that E. coli had difficulty reproducing the large DNA segments, so they tu...

PharmSource and BioProcess Technology Consultants Announce Publication of New Market Report: Cell Culture Manufacturing Capacity: Trends and Outlook through 2013

...utical industry and the industry's foremost expert on cell culture manufacturing. The company specializes in helping clients speed their products from clone to commercial®. Visit BioProcess Technology Consultants on the web at http://www.bioprocessconsultants.com or contact them at 978-266-9113. ...

OriGene Announces 37,000 ORF cDNA Clones in a GFP Vector

...ene with GFP to monitor protein expression level, detect exact cellular location, and study protein-protein interaction. As a leading full length cDNA clone provider, OriGene is committed to providing expanded utilities for these clones in life science research and drug discovery. About OriGene Based i...

Cyntellect Launches CellXpress(TM) System to Boost Productivity of Biopharmaceutical Process Development

...M). The automated system brings new improvements in speed, cost and optimal clone selection for cell line development. Biopharmaceutical teams developing man... leaving behind only the best cell. This purification results in a verified clone that is very likely to generate a highly secreting cell lines. The imaging ...

Sigma-Aldrich Joins Phase 2 of The RNAi Consortium to Validate shRNA Libraries

...ements to the library and its applications." The current MISSION(R) shRNA clone libraries comprise a comprehensive collection of more than 160,000 pre-clon... genes (MISSION TRC-Mm1.0). For more information about the MISSION(R) shRNA clone collections, please visit us online at http://www.sigma.com/trc2 . About...

The most natural drug

...niversity stands to revolutionize the process. In an advance online publication in Nature, the researchers describe a method that can identify and clone human antibodies specifically tailored to fight infections. The new technology holds the potential to quickly and effectively create new treatments fo...

China Agro-Technology Appoints New CEO

...anagement and operation of Genetically Engineered plantations, the non-tube clone plantations and the marketing of the proprietary rights and technological k...cutting-edge Non-tube Leaf Culture Technology is able to produce 60 million clone plants in 24 months for 20,000 ha of land from only one nursery station. ...

Stemagen First to Create Cloned Human Embryos From Adult Cells

...be clones based on DNA fingerprinting demonstrating the presence of the skin cell donor DNA in the blastocyst, while one was further confirmed to be a clone by an additional mitochondrial DNA (mtDNA) analysis which revealed the presence of oocyte donor mtDNA without any oocyte donor nuclear DNA. For techni...

Animal Protection Groups Condemn FDA's Endorsement of Animal Cloning

...ncluded in the FDA's assessment reveal, for example, that 28 percent of cow clone pregnancies suffer from hydrops, an often fatal condition in which the moth...ms." Even older clones who appear healthy have been known to develop "adult clone sudden death syndrome," which casts doubt that cloned animals can ever be c...

Statement on the Release of the European Food Safety Assessment on the Safety of Food Derived From Clones and Their Offspring in the Food Supply

...the beef, pork, dairy, retail and food service industries." Additional Resources: Link to EFSA Report: http://tinyurl.com/27lka7 Link to clone tracking information: http://www.clonesafety.org/cloning/scm Background on International Cloning Regulation: -- Last year the European Commi...

Discovery Institute Bioethicist Lauds Breakthrough in Stem Cells Research that Eliminates Need for Human Cloning

...rogrammed adult cells back to an embryonic-like state, opening the possibility of patient-specific medical treatments and research without the need to clone human life. Wesley J. Smith, the Discovery Institute's Senior Fellow in Bioethics and author of Consumer's Guide to a Brave New World, hailed the bre...

K-State's Subbaratnam Muthukrishnan and Barbara Valent Named Fellows of the American Association for the Advancement of Science

...ice blast disease as an easily manipulated model system to understand how plants and fungi interact to lead to disease or resistance. She was first to clone and study both fungus and rice genes that determine the outcome of this interaction. Valent is applying functional genomics and advanced cell biology ...

Michigan Catholic Conference Responds to Formation of Embryonic Stem Cell Research Ballot Committee

...e "Stem Cell Research BQC": "In order to protect human life at its most vulnerable stage, any proposal, legislative or ballot related, that seeks to clone and/or kill human embryos in this state must be defeated. According to a statewide Public Opinion Strategies poll, 77 percent of Michigan residents op...

3 strikes, you're out? Refiling of Amendment to Change Constitution to Criminalize Stem Cell Cures Shows Trouble with Anti-Cures Cause

...ntees equal access to stem cell research, treatment and cures allowed under federal law and available to other Americans. It also makes any attempt to clone a human being a felony crime, punishable by up to 15 years in prison and a $250,000 fine. The proposed initiative's plan to redefine stem cell researc...

New Initiative Has Shocking Effect: Thousands of Missourians with Abnormal Chromosome Structures Are Not Human

...ntees equal access to stem cell research, treatment and cures allowed under federal law and available to other Americans. It also makes any attempt to clone a human being a felony crime, punishable by up to 15 years in prison and a $250,000 fine. The proposed initiative's plan to redefine "cloning" is real...

Molecular Cloning Systems Gets Distributor

...ome. The technology utilizes a series of low-background vectors designed to clone sequences that are difficult to clone with conventional vectors. Our collaboration with Lucigen to leverage t...

Molecular Cloning Systems Gets Distributor

...ome. The technology utilizes a series of low-background vectors designed to clone sequences that are difficult to clone with conventional vectors. Our collaboration with Lucigen to leverage t...

Anti-Cloning Petition Drive Launched in Wisconsin

...t his inauguration. Democrat Gary Sherman, a state representative from northern Wisconsin, has introduced a bill that strongly condemns efforts to clone humans, but protects what he calls legitimate research -- including stem cell projects. "This is one of the worst ideas in the history of science," Sh...

Lucigen Receives Funding, Breaks New Ground with Enzyme Research

...le enzymes found within microbes that live in extreme environments, like hot springs and polar ice cores, for researchers. The companys technology can clone microbes from a single cell, as compared to the current industry standard of approximately 10 billion cells. [Our techniques] are a billion times ...

How Wisconsin Can Maintain Its Lead in Stem Cell Research

...er be tampered with. So there was understandable culture clash in Seoul last week when South Korean researchers announced they had become the first to clone a mature, human embryo and then collect from it master stem cells in a process that may someday let patients grow their own replacement tissues. F...

Lucigen aims to fill holes in genetic maps with new technique

...iddleton, Wis. -- Lucigen Corporation , which provides molecular cloning products to genetics researchers, has been awarded a patent on a new way to clone genes that may solve a persistent problem in genetics. Researchers often find missing pieces or gaps when they analyze genes using standard method...

The birth of biotech: San Francisco, Boston, Geneva or Chicago?

... go with Amgen. Amgen scientist Fu-Kuen Lin spent two years working out the process for sequencing the EPO protein and another year to sequence and clone the process using Chinese hamster ovary cells. Lins first patent was filed on Dec. 13, 1983 with other patents filed on the process for producing r...

Human cloning plans get blessing from British government

...ed Dolly the sheep, has been granted a license by the British government to clone human embryos is sure to spark fresh hopes among disease sufferers, scienti...rate stem-cell lines to study motor neuron disease (MND). The team plans to clone embryos to generate stem cells that will in turn become motor neurons with ...

The Attack of the Clones: No, its not a summer movie

...at mirror current practice in Wisconsin. With more reflection and honest debate on all sides, perhaps Wisconsin could produce a peaceful ending to the clone Wars, as well. Tom Still is president of the Wisconsin Technology Council and the Wisconsin Innovation Network. He is the former associate edito...

Stem-cell debate, wireless access and other endeavors heat up

... have been the discussions over cloning. The term draws up images of fully-grown cloned people in science-fiction movies, though in the lab scientists clone cells or embryos to work with. It has also moved beyond a matter of scientific ethics and become a partisan political debate. Republicans would like t...

Fact and friction: Putting election-year stem cell claims under the microscope

...notes. Mark Green says he does not believe "anyone should be allowed to clone a human being Jim Doyle does." Is it true that Doyle wants to clone a person - perhaps even a campaign contributor? The answer depends on y...

Madison business receives $115,000 of new state biofuels funding

...ng technology, which will be used to produce enzymes used in the development of alterative fuels. "Our tools are cloning tools," Biondi stated. "We clone genetic material previously thought to be unclonable, and with very little material to work with." From this material, the company will extract enz...

Lucigen Corp. enters global licensing agreement

...mics cloning technologies to produce new types of enzymes from rare microorganisms isolated from boiling hot springs. The use of bacterial cells to clone and express genes is common in research, diagnostics, and biopharmaceuticals. However, many recombinant proteins can't be produced this way because th...

Lucigen receives $750K grant to boost biofuel yields

...tion from soybeans and biomass. Lucigen has been bioprospecting hot springs and other high-temperature environments by using proprietary methods to clone and screen the genomes of rare bacteria and viruses for novel enzymes. Similar but less efficient high-temperature enzymes are used in automated D...

Amplification Efficiency of TaqMan Gene Expression Assays

... We suggest you use: PCR product (be very careful to avoid contamination of the laboratory space with amplified DNA); A cDNA clone at a high template concentration; or Genomic DNA (if your TaqMan probes and primers are designed within an exon) References ...

Laser Capture Microdissection (LCM) and Expression Profiling of Long-Range Projection Neurons

... for 45 seconds, 72C for 45 seconds. 2. Amplified DNA was purified and cloned into the pCR II-TOPO vector (Invitrogen). The identity of each clone was confirmed by DNA sequencing. 3. Antisense probes were generated by restriction enzyme digestion and transcription from the T7 promoter in...

Neurite outgrowth cell-by-cell analysis using the IN Cell Developer Toolbox

...red Mouse renoblastoma neuro-2a (N2a) cells (ATCC)

Retinoic acid (Sigma)

Monoclonal anti-α-tubulin clone DM1A (Sigma)

Anti-mouse IgG-FITC antibody (Sigma)

Hoechst™33342 (Molecular Probes)

Assa...

GenomiPhi: protocol for BAC amplification and fabrication of slides for array CGH

...mic arrays. Ligation-mediated PCR is one of several current strategies being used for the generation of genomic arrays. In this process, a genomic DNA clone is digested with a restriction enzyme and a universal primer adaptor is ligated to serve as a priming site for PCR amplification. A different method e...

Monitoring Bacterial Genetic Diversity in a Freshwater Lake Using TTGE and DNA Sequence Analysis

...al samples. Additionally, sequencing the gel-resolved DNA enables phylogenetic analysis of PCR products, eliminating the need to clone DNA fragments and, thus. simplifying our analysis. References 1. Hastings, R. C. et al., Appl. Environ. ...

Examples of Electro-Transfected Eukaryotic and Prokaryotic Cells

...S-1, COS-7, CV-1, Vero) Mouse, 3T3, embryo Mouse, 32d, myeloma Mouse, B Mouse, BALB/c 3T3, clone A31, fibroblast, embryo Mouse, BbSutA, hematopoietic Mouse, C127, fibroblast, mammary tumor Mouse, C2, muscle m...

Electrophoretic Karyotypes of Wine Strains of Saccharomyces cerevisiae

...tially resolved doublet corresponding to laboratory band 11. The karyotype of the genetically purified and presumably homozygous clone 522XA (lane D) has a different profile than its progenitor, UCD 522A. The band migrating just ahead of laboratory band 3 is absent ...
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