Chromatography

Purification of Murine IgG1 Using UNOsphere S and CHT Ceramic Hydroxyapatite Chromatography, Rev A

Ceramic Hydroxyapatite Chromatography, Rev A Henry Lai, PhD, and Samuel G Franklin, PhD, Bio-Rad Laboratories, Inc., 2000 Alfred Nobel Drive, Hercules, CA 94547 USA Immunoglobulins (IgGs) fr...

Rapid Chromatography Optimization by pH Scouting Using the New BioLogic Maximizer Valve System, Rev A

Chris Moran, Bio-Rad Laboratories, Inc., 2000 Alfred Nobel Dr., Hercules, CA 94547 Fast and efficient protein purification is essential to explore the structure and function of proteins in the human proteome. Successful protein purification strategies use experimental conditions where...

Rapid Method Development With the BioLogic DuoFlow Chromatography System for the Purification of His-Tagged Proteins

Fang-Fang Wu, Xuemei He, and David R Nau, Bio-Rad Laboratories, Inc., Hercules, CA 94547 USA Immobilized metal affinity chromatography (IMAC) is a powerful technique that can be used for the efficient purification of recombinant histidine-tagged (His-tagged) proteins from a variety of ex...

Separation Reproducibility With the BioLogic Chromatography Systems

Kevin Thornton, PhD, Bio-Rad Laboratories, Inc., Hercules, CA 94547 USA In chromatographic separations, sample component elution times are often used both for identification and to program automated chromatography steps like fraction collection. Therefore, the ability to obtain reproducible...

Determination of N-nitrosamines in Baby Bottle Rubber Teats by Liquid Chromatography-Atmospheric Pressure Chemical Ionization Mass Spectrometry

This application note illustrates an improved method to quantitate Nnitrosamines in baby bottle teats using APCI-MS. Six rubber nipple formulations were analyzed. Two formulations were composed of natural rubber, two were composed of a synthetic silicone polymer and the remaining two were composed of a synthetic polypropylene. The natural rubber formulations were found to have a signi...

Separation and Quantitation of R and S Enantiomers using Normal Phase Chromatography on an API 3000 LC/MS/MS System

In recent years, pharmaceutical companies have placed greater importance on examining the stereoisomeric composition of drugs having a chiral center. Isomerspecific pharmaceuti...

Using THERMAL DESORPTION GAS CHROMATOGRAPHY for the identification of fragrance and other components in cosmetic products

Because of the solid or viscous nature of these formul...

Use of On-line Size Exclusion Chromatography (SEC) for Sample Clean-up in LC/MS Analyses

Use of LC/MS has experienced a dramatic increase in applications throughout the drug discovery and development process, from high-throughput screening...

ANALYSIS OF AMINO ACIDS BY REVERSED PHASE CHROMATOGRAPHY WITH PRECOLUMN DERIVATISATION AND UV/VISIBLE DETECTION

Amino Acids, RP-HPLC, Precolumn derivatisation, o-Phthalaldehyde (OPA), 2-Mercaptopropionic acid, Ultraviolet detection. Introduction: Underivatised amino acids do not significantly absorb light in the UV/visible spectrum. In order to increase sensitivity, their analysis normally includes a derivatisation step in whic...

Practical Aspects of Evaluation of Chromatographic Data in Size Exclusion Chromatography

e-mail: vins@dataapex.com Duan Berek, Polymer Institute SAV, Bratislava, Slovak Republic Determination of MW distribution of polymers by the liquid chromatographic methods with concentra...

Separation of Peptides by Hydrophilic Interaction Chromatography Using TSKgel Amide-80

Authors: T.J. Higley, Tosoh Bioscience LLC, Tatsunari Yoshida, Tosoh Corporation The TSKgel Amide-80 column provides excellent selectivity for separating peptides in hydrophilic interaction chromatography due to polar carbamoyl functionalities bonded to a silica base material....