CTO

A New C-Terminal GST Vector for Protein Production in S. pombe

Stratagene Stratagene has constructed and tested the new pESP-3 vector, which can beused for C-terminal fusion with glutathione-S-transferase (GST) and for protein production in Schizosaccharomyces pombe....

Generate Adenovirus Vectors in E. coli by Homologous Recombination with the AdEasy Adenoviral Vector System

Efficient gene transfer and high-level protein expression within a varietyof cells Stratagene Recombinant adenoviruses provide a versatile means of gene delivery andexpression. We describe the AdEasy adenoviral vectorsystem, whichsimp...

An Epitope Tagging Vector for Gene Expression in Mammalian Cells

Stratagene The pCMV-Tag1 vector is an epitope tagging vector designed for geneexpression in mammalian cells. A target gene inserted into the pCMV-Tag1 vectorca...

Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in Mammalian Cells

Stratagene mammalian expressionsystem allows tightc...

Epitope-Tagging Vectors for Functional Analysis in Yeast

Stratagene Stratagene has developed a series of epitope-tagging vectors to expressand functionally analyze eukaryotic genes in the budding yeast Saccharomyce...

New Mammalian Expression Vectors Employ Stable, High-Level Fluorescence Humanized Renilla GFP Reporter

hrGFP expression vectors for monitoring gene expression and localizing proteins in vivo New Mammalian Expression Vectors Employ Stable, High-Level Fluorescence Humanized Renilla GFP Reporter...

Novel PCR Enhancing Factor Improves Performance of Pfu DNA Polymerase

pfuturbo DNA polymerase for robust, high-fidelity PCRamplification Holly Hogrefe Brad Scott Kirk Nielson ValerieHedden Connie Hansen Janice Cline Frances Bai Jeff Amberg RondaAllen Mark Madden Stratagene an improved version of Pf...

Efficiently Insert Unique Restriction Sites into Plasmid Vectors

Stratagene A 28-base insertion was introduced into a plasmid vector using theQuikChange site-directed mutagenesis kit.* Theinsertion created three unique restriction enzyme digestion sites. Plasmid DNAisolated from 9 o...

High-Efficiency Site-Directed Mutagenesis of Large Plasmid Vectors

QuikChange XL: a highly versatile kit for site-directed mutagenesis oflarge plasmids Michael Borns Peter Vaillancourt Holly Hogrefe Jeff Braman Stratagene The QuikChange site-directed mutagenesis kit* characteristicallygenerates mutagenesis efficiencies exceeding 80%. We have created a new kit formutagenesis of large plasmid...

A New Lambda Vector for Mammalian Expression

-CMV vector for efficient library constructionand mammalian expression Quinn Lu Tanya Hosfield Cherie Dewar Tim Sanchez Mike Kobrin Stratagene -CMVvector,* contains the left and right arms of the Lambda ZAP II vector...

Versatile Reporter Vectors for Monitoring Viral Transduction

Determine retroviral transduction efficiency and gene expression invirtually any target cell line Stratagene , pFB-Neo-LacZ <font...

hNT BlueNeurons Express b-Galactosidase at High Levels

-Galactosidase at HighLevels Layton BioScience, Inc. Gilroy, California Stratagene's hNT BlueNeurons express beta-galactosidase at h...

High-Titer Retroviral Vectors for Gene Delivery

High-TiterRetroviral Vectors for Gene Delivery Stratagene The high-titer retroviral vectors pFB and pFB-Neo can be used with anyMoloney Murine L...

Improved Vectors for PathDetect Trans-Reporting Systems

Stratagene Stratagenes PathDetect in vivo signal transduction pathwayreporting systems can be used to assess the in vivo activation of signaltransduction pathways. To expand the utility of these systems, Stratagene isreplacing the pFA-cJun...

Mammalian and Bacterial Expression in One Vector

Novel pdual expression vector for high-level expression in bothmammalian and bacterial cells Stratagene Stratagene has developed the pdual expression vector,* which permitshigh-level expression of heterologous genes in both eukaryotic and prokaryoticsystems. The vector contains the promoter an...

Mammalian Expression Vector for Efficient Cloning of PCR Fragments

Tanya Hosfield Kerstien Padgett Tim Sanchez QuinnLu Stratagene Cloning Systems, Inc. cloningstrategy by introducing a new vector for high-efficiency cloning of PCRfragme...

Mammalian Expression Vectors with Interchangeable Selectable Markers

Site-specific recombinase-based vector system Stratagene system of eukaryotic expression vectors offers unparalleled convenience. The drug-resistance genes of these vectors can be exchanged without subcloning. Through site-specific recombination, hygromycin, puromycin,...

Sequence-Validated and Expression-Tested Human cDNA in a Dual Expression Vector

Study protein function more efficiently with standardized clones Rebecca L. Mullinax Heidi Davis David T. Wong Kelly Wynne Vilma Nioko Leonardo DeLeon SanDEe Soares Joseph A. Sorge Edward Marsh Stratagene Phenogenex Stratagene now offers s...

Lactococcus lactis

Lactococcus lactis MG1363...

Lactococcus lactis

Lactococcus lactis MG1363...

Critical factors for successful microarray and real-time PCR analyses

QIAGEN GmbH, Hilden, Germany Gene expression profiling at the RNA level has been significantly facilitated by microarray analysis and quantitative real-time RT-PCR. Microarray analysis offers the advantage of profiling expression levels of hundreds or thousands...

Transfection Reagent Selector Kit Handbook

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Your Data: Silencing of a Pro-apoptotic Transcription Factor Using Synthetic siRNA

One of the RNA silencing products available from Ambion is a kit for the synthesis of siRNA, the <A href="http://www.ambion.com/catalog/CatNum.php?1620">Silencer <A href="http://www.ambion.com/catalog/CatNum.php?16000"> siRNA Construction Kit . While this kit is very cost effective...

A highly sensitive ATP-luminescence assay using VICTOR2 with the dispenser option

In high sensitivity flash type luminescence assays the measurement is done just after dispensing the activating reagent or after a short delay time. Over the past years luminescence substrates have been improved towards providing more stable signal. These so-called glow type substrates allow microplates to be counted without dispensors. Ho...

Bruker AXS unveils new X-ray detector

The new systems were unveiled at the 2005 International Union of Crystallography m...

Bone Care marks solid quarter on Hectorol sales jump

Hectorol is a Vitamin D-based drug used to lower abnormally high levels of parathyroid hormone in patients with chronic kidney disease. Gross margins run about 75 percent....

The doctor is in: Thompson prescribes better health information technology

The Wisconsin Technology Network is committed to helping improve patient safety and healthcare through the development and deployment of technology. We salute the administration and the efforts of Secretary Thompson and his agency to help spread the adoption health technology. If you are interested in learning more about this topic you should attend the following event....

Sensenbrenner and USPTO Director highlight benefits of technology transfer

Hartland, Wis. At a meeting Monday highlighting university-business partnerships in Wisconsin, , a medical imaging company based in Hartland, Wis., demonstrated its latest tool for assessing cardiovascular health to John Dudas, acting under secretary of commerce for the <a href="http://www.uspto.gov/" target=...

FDA approves Bone Cares Hectorol for treatment of kidney disease

Middleton, Wis. -- announced Monday the U.S. Food and Drug Administration approved a new indication and strength for Hectorol (doxercalciferol) Capsules. Hectorol, a pro-hormone vitamin D2 analog, is currently approved for the treatment of secondary hyperparathyroidism (SHPT) in dialysis patients in the United Sta...

Computer Card Supports Solo Doctor

Oconomowoc, WI-Patrick O'Malley, D.O. established his practice, O'Malley Family Medicine, in Oconomowoc, Wis. (outside Milwaukee) in 2001.He envisioned that practicing ndependently would enable him to provide personalized care to his patients. But, how can a solo practitioner build a thriving practice in today's healthcare industry? "Keeping my overhead low is essential," says O'Malley. "That's w...

Protector RNase Inhibitor

from rat lung, recombinant Catalog Number Pack Size 3335399 2000 units 3335402 10000 units (5 vials of 2000 U...

Protector RNase Inhibitor Enhance the Protection of RNA against Degradation

Is a new type of RNase inhibitor and inhibits a wide known spectrum ofRNases. Does not interfere with the performance of enzymes in an assay - evenwhen high amounts (e.g., up to 16-fold the standard concentr...

Delivering siRNA Using Adenoviral Vectors

Efficiently deliver an siRNA expression construct into a variety of mammalian cells and organisms Ideal for many primary and other difficult to transfect cells Positive Control Oligonucleotide Insert an...

Drive Long-Term Silencing with CMV Vectors

Ambion's newest line of plasmid siRNA expression vectors, p 4.1-CMV, feature polymerase (pol) II promoters that drive transcription of both an siRNA template and an antibiotic resistance gene. The CMV promoter is used to drive siRNA expression. This...

pSilencer 4.1-CMV: Versatile Vectors for Expression of siRNA, miRNA, and mRNA

4.1-CMV plasmid vectors are designed for long-term gene silencing experiments in a broad range of cell lines. The vectors carry an RNA polymerase II-type CMV promoter (human cytomegalovirus immediate-early promoter) and an optimized SV40...

More siRNA Vectors for RNA Interference

Small Interfering RNAs (siRNAs) are short, double-stranded RNA molecules that can target complementary mRNAs for degradation via a cellular process termed RNA interference (RNAi) (Elbashir et al., 2001a). Recent publications have described the use of vectors expressing functional siRNAs to enable long-term...

Selecting siRNA Sequences to Incorporate into the pSilencer Vectors

The first step in preparing a plasmid for siRNA experiments is to identify target sequences in the gene of interest that are susceptible to siRNA-induced degradation. We have found that a little more than half of the siRNAs provide at least a 50% reduction in target mRNA levels and approximately 1 out of 4 siRNAs...

siRNA Expression Vectors with Selectable Markers

siRNA expression vectors are now available, each with an antibiotic resistance gene to facilitate selection in mammalian cultured cells. These new vectors are ideal for the long-term study of gene specific kno...

Superior Gene Silencing Using Adenoviral Vectors

Mediating RNA interference (RNAi) with recombinant adenovirus expressing siRNAs has only recently been described in the literature (1). Recombinant adenoviruses have several advantages over other methods of nucleic acid delivery. Namely, these viruses can infect dividing and...

Your Data: Use of pSilencer 1.0-U6 siRNA Expression Vector in the Analysis of Synaptotagmin Location and Function

Iezzi M, Kouri G, Fukuda M, Wollheim CB (2004) Synaptotagmin V and IX isoforms control Ca J Cell Sci In a recently published paper in the Journal of Cell Science, Iezzi and colleagues used Ambions...