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BAC in Biological Technology

Automation of the Eppendorf Perfectprep BAC 96 DNA Kit on the Caliper Life Sciences Sciclone ALH 3000

Lynn Jordan, James Batchelor, and Ed Alderman Applied Science and Technology Department, Caliper Life Sciences Inc., Hopkinton, MA 01748 Kelly M. Clark, Joseph A. Hensley and Robert P. Weisbein Brinkmann An Eppendorf Company, Westbury, NY Jen...

GenomiPhi: protocol for BAC amplification and fabrication of slides for array CGH

Prasad D.K. Dhulipala 1 , Rao S. Takkellapati 1 , Kate Lavrenov 1 , Scott Hamilton 1 , and Jessica Pole 2 1 GE Healthcare, Piscataway, NJ, USA 2 Hutchison/MRC Research Centre, University of Cambridge, Cambridge, UK ...

Susan G. Komen for the Cure Announces Recipients of 2007 Komen Brinker Award for Scientific Distinction

... current patient management. His list of credits also includes brdU/DNA analysis of cell cycle progression; comparative genomic hybribidization (CGH); bac End Sequencing (BES) and, more recently, nanotechnology. Dr. Gray was an early adopter of technologies such as transcriptional profiling, high throu...

Empire Genomics and Reprogenetics Join Forces to Improve Preimplantation Genetic Diagnosis (PGD)

...s evolved array-based Comparative Genomic Hybridization (aCGH) by developing microarrays that utilize discrete segments of DNA generated from the RPCI bac clones. These are of considerable diagnostic value to early stage disease detection and treatment, and streamline the process by eliminating the need ...

New method of selecting DNA for resequencing accelerates discovery of subtle DNA variations

...egative consequences but are not so easily discovered. Other methods for isolating and studying a particular region of the genome, such as PCR and bac cloning (bacterial artificial chromosomes) are comparatively labor intensive, difficult for single laboratories to scale to large sections of the geno...

Amplified Fragment Length Polymorphism (AFLP) Analysis on Applied Biosystems Capillary Electrophoresis Systems

...eature to verify that no poor quality data was used. Condition Numbers and Quality Score boundaries should be optimized for each dye set. Refer to the bac Fingerprinting on the Applied Biosystems 3730/3730xl DNA Analyzer application note (S/N 107AP04-01) for more detail on this procedure. The p...

GenomiPhi amplification of FFPE tissue DNA for array CGH

...h Cy™3 and Cy5 dyes, and hybridized to human bac microarrays. Commercially available human bac arrays from Spectral Genomics were chosen to verif...y comparing microarray hybridization data on human bac arrays. The results show that the GenomiPhi method...

Using the VersaFluor Fluorometer to Quantitate GUS Expression in Plant Tissues Bombarded With the Biolistic PDS-1000/He System

...ed abscission zones. GUS expression is used as an internal control in experiments designed to identify regulatory regions in the bac gene promoter. An example of the approach used and results obtained is described below. In addition to quantification of GUS act...

Acrylamide Polymerization A Practical Approach

...proteins or nucleic acids. These crosslinkers include DATD (diallyltartardiamide), DHEBA (dihydroxyethylene-bis-acrylamide), and bac (bis-acrylylcystamine). Alternative crosslinkers may be more or less reactive in polymerization than bis. Therefore, some adjustment in the...

COSMCPREP HIGH AND LOW COPY PLASMID PURIFICATION

... BACs. CosMCPrep-purified plasmids are high quality and result in improved sequencing performance (Figure 2). Figure 3 shows a bac end sequence with a Phred20 read length of 700bp. ...

Genotyping Single Nucleotide Polymorphisms with Molecular Beacons

...(Research Genetics) were selected from the region flanking the SNP11 in the bac and generated a 144-bp-long amplicon. Human genomic DNAs were isolated ...encing: We amplified fragments with PCR primers flanking the 300- to 600-bp bac genomic sequence of interest in four family members, ran the PCR products i...
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