Automation of the Eppendorf Perfectprep BAC 96 DNA Kit on the Caliper Life Sciences Sciclone ALH 3000
Lynn Jordan, James Batchelor, and Ed Alderman
Applied Science and Technology Department, Caliper Life Sciences Inc., Hopkinton, MA 01748
Kelly M. Clark, Joseph A. Hensley and Robert P. Weisbein
Brinkmann An Eppendorf Company, Westbury, NY
GenomiPhi: protocol for BAC amplification and fabrication of slides for array CGH
Prasad D.K. Dhulipala 1 , Rao S. Takkellapati 1 , Kate Lavrenov 1 , Scott Hamilton 1 , and Jessica Pole 2
1 GE Healthcare, Piscataway, NJ, USA
2 Hutchison/MRC Research Centre, University of Cambridge, Cambridge, UK
Susan G. Komen for the Cure Announces Recipients of 2007 Komen Brinker Award for Scientific Distinction
... current patient management. His list of credits also includes
brdU/DNA analysis of cell cycle progression; comparative genomic
hybribidization (CGH); bac
End Sequencing (BES) and, more recently,
Dr. Gray was an early adopter of technologies such as transcriptional
profiling, high throu...
Empire Genomics and Reprogenetics Join Forces to Improve Preimplantation Genetic Diagnosis (PGD)
...s evolved array-based Comparative Genomic Hybridization
(aCGH) by developing microarrays that utilize discrete segments of DNA
generated from the RPCI bac
clones. These are of considerable diagnostic
value to early stage disease detection and treatment, and streamline the
process by eliminating the need ...
New method of selecting DNA for resequencing accelerates discovery of subtle DNA variations
...egative consequences but are not so easily discovered.
Other methods for isolating and studying a particular region of the genome, such as PCR and bac
cloning (bacterial artificial chromosomes) are comparatively labor intensive, difficult for single laboratories to scale to large sections of the geno...
Amplified Fragment Length Polymorphism (AFLP) Analysis on Applied Biosystems Capillary Electrophoresis Systems
...eature to verify that no poor quality data was used. Condition Numbers and Quality Score boundaries should be optimized for each dye set. Refer to the bac
Fingerprinting on the Applied Biosystems 3730/3730xl DNA Analyzer application note (S/N 107AP04-01) for more detail on this procedure.
GenomiPhi amplification of FFPE tissue DNA for array CGH
...h Cy™3 and Cy5 dyes, and hybridized to human bac
microarrays. Commercially available human bac
arrays from Spectral Genomics were chosen to verif...y comparing microarray hybridization data on human bac
arrays. The results show that the GenomiPhi method...
Using the VersaFluor Fluorometer to Quantitate GUS Expression in Plant Tissues Bombarded With the Biolistic PDS-1000/He System
...ed abscission zones. GUS expression
is used as an internal control in experiments designed to identify regulatory
regions in the bac
gene promoter. An example of the approach used and
results obtained is described below. In addition to quantification of
Acrylamide Polymerization A Practical Approach
...proteins or nucleic acids. These crosslinkers include DATD
(diallyltartardiamide), DHEBA (dihydroxyethylene-bis-acrylamide), and
(bis-acrylylcystamine). Alternative crosslinkers may be more or less
reactive in polymerization than bis. Therefore, some adjustment in the...
COSMCPREP HIGH AND LOW COPY PLASMID PURIFICATION
... BACs. CosMCPrep-purified plasmids are high quality and result in
improved sequencing performance (Figure 2). Figure 3 shows a bac
end sequence with a Phred20 read length of 700bp.
Genotyping Single Nucleotide Polymorphisms with Molecular Beacons
...(Research Genetics) were selected from the region flanking the SNP11 in the bac
and generated a 144-bp-long amplicon. Human genomic DNAs were isolated
...encing: We amplified fragments with PCR primers flanking the 300- to 600-bp bac
genomic sequence of interest in four family members, ran the PCR products i...