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Fluorescence-Based Single-Tube Assays to Rapidly Detect Human Gene Mutations
Xiuyuan Hu Beti Belachew Lingyu Chen HaoqiangHuang Jason Zhang Stratagene can distinguish human DNA specimens that differ by a few nucleotides or only asingle nucl...LiquiChip Kits for bead-based cytokine and kinase assays
The LiquiChip Human and Mouse 10-Cytokine Kits and Ser/Thr Kinase Kit provide fast and accurate quantification of a range of cytokines and serine/threonine kinases. LiquiChip 10-Cytokine Kits enable simultaneous multiplex assay of 10 different cytokines using bead-based xMAP technology. The LiquiChip Ser/Thr Kinase Kit...Nuclease Protection Assays: The Basics
<A href="#1">What is an NPA? <A href="#2">Advantages > <A href="#3">Limitations > <A href="#4">Steps <br...The cell proliferation assay is an important tool in the assessment of the immune system function and in the search for new drugs. Productivity with this technique has been limited by the lack of automated, high throughput sample processing and counting equipment. The MicroMate harvester and the TopCount Microplate Scintillation Counter help r...
Receptor Binding Assays using Fluorescence Polarization Detection: the [FP]2TM Characterized Tracers
. The process is typically highly automated and can provide screening rates over 100,000 samples in a 24 hour period. To achieve this, assays must be simple, p...Development of Radioligand Binding Assays for the Motilin Receptor Using ScreenReady Targets.
Recent advances in combinatorial chemistry and genomics programs have led to an unprecedented increase in both the number of compounds and potential drug targets that can be screened. The development of automated liquid h...Evelyn McGown, Ph.D. and Anna Lam, B.S. Molecular Devices Corporation, 8/99 Modern assays for proteolytic activity typically use synthetic chromogenic or flu-orogenic peptide substrates. Enzyme selectivity is obtained by choosing a peptide sequence uniquely recognized by the catalytic site of a particular enzy...
Gq-coupled Receptor Assays Using FLEXstation
Departments of...Gq-coupled receptor assays using FLEXstation: M1 muscarinic receptor
SIMPLE, RAPID EXPERIMENTAL SET-UP AND DATA ANALYSIS DETERMINE EC EXCELLENT FOR ASSAY DEVELOP-MENT AS DEMONSTRATED BY Z FACTOR INTROD...Gq-coupled receptor assays: a comparison of FLEXstation and FLIPR
SIMPLE, FAST EXPERIMENTAL SET-UP Activation of G -coupled...Calcium Flux Assays using the LabChip 3000
The LabChip microfluidic assay accesses test compounds from a micropl...Matched siRNAs and Assays: Ambion + Applied Biosystems = RNAi Success
Ambion and Applied Biosystems have joined forces to provide a complete convenient, solution for performing gene silencing experiments and validating the results by real-time RT-PCR. Ambion's Validated and Pre-designed siRNAs eliminate the guesswork--and the tedious labwork--associated with si...Using Validated siRNAs in Functional Genomic Assays
Collections of siRNAs targeting key human genes are ideal for studying protein function in cells. To identify genes involved in a given cellular process, cells can be transfected with different siRNAs and assayed for distinct response profiles such as cell cycle arrest....and Aiguo Zhang, 1 Department of Molecular and Clinical Medicine, Division of Paediatrics, Linkping University, Sweden, and Bio-Rad Laboratories, Inc., 2000 Alfred Nobel Drive, Hercules, CA 94547 USA...
Principles of Curve Fitting for Multiplex Sandwich Immunoassays, Rev B
Diana Davis, PhD, Aiguo Zhang, PhD, Chloe Etienne, Ivan Huang,and Michele Malit, Bio-Rad Laboratories, Inc., 2000 Alfred Nobel Drive,Hercules, CA 94547 USA Bio-Plex cytokine assays are bead-based multiplex sandwich immunoassays. The concentrations of analytes in a Bio-Plex cytokine immunoassay are...Selection of Standards for Bio-Plex Cytokine Assays, Rev A
Diana Davis, Aiguo Zhang, Jim Torrence, and Emily Dale, Bio-Rad Laboratories,Inc., 2000 Alfred Nobel Drive, Hercules, CA 94547 USA Bio-Plex cytokine assays are bead-based quantitative multiplex immunoassays. The concentrations of analytes in these assays are quantitated using a calibration or...Feasibility of Multiplexing Bio-Plex Total Target and Phosphoprotein Assays
Qian Gao, Joella Blas, and Efthalia Gerasimopoulos, Bio-Rad Laboratories, Inc.,Hercules, CA 94547 USA Bio-Plex assays allow detection of mutiple target proteins in a small volume of sample. The assays are based on detection of signal from tagged fluorescent beads (xMAP technology) that have bo...The Gradient Feature: Use in Optimization of Allelic Discrimination Assays, Rev B
Luis A Ugozzoli, Bio-Rad Laboratories, Inc., 2000 Alfred Nobel Drive,Hercules, CA 94547 USA PCR has been greatly refined since its advent 16 years ago. Several improvements in the technology and associated equipment have significantly changed the way PCR is performe...The iCycler iQ Detection System for TaqMan Assays, Rev B
Faye Boeckman, Keith Hamby, and Larissa Tan, Bio-Rad Laboratories, 2000 Alfred Nobel Drive, Hercules, CA 94547 USA Specific fluorescent oligonucleotide probes allow for real-time monitoring of the polymerase chain reaction (PCR*). One popular probe strategy is the TaqMan assay...Is Z' factor the best assessment for the quality of cellular assays delivering higher content?
Amersham Biosciences UK Limited, Amersham Place, Little Chalfont, Buckinghamshire HP7 9NA England As high throughput imaging systems, analysis al...Apoptosis represents a gene-directed, morphologically and biochemically distinct form of programmed cell death. Inappropriate control of apoptosis is associated with a variety of disease states such as cancer, AIDS, neurode...
Micronucleus assays • toxicology • image analysis • IN Cell Analyzer Micronucleus induction is a key characteristic of genotoxic compounds and analysis of micronuclei formation resulting from DNA strand breakage (clastogens) or interference with chromosome segregation (aneugens) is an important component of toxic...
Use of BRET2 luminescence cell-based assays with LEADseeker Multimodality Imaging System
Competitive pressure is forcing organizations to continually increase screening throughput. Yet accuracy and sensitivity remain vital concerns, particularly for common screens such as luminescence cell-based ass...As the study of protein biomarkers increases in importance, extensive lists of candidate markers are being developed based on protein pathway information and discovery-based proteomics experiments. Even larger numbers of candidate genomic markers exist from DNA transcriptional profiling based on microarray-based gene expressio...
Mix-and-Read, Bead- and Cell-Based Immunoassays Using the FMAT 8100 HTS System
Immunoassays are valuable and widely used tools in the biological sciences. In drug discovery research, immunoassays are used to measure secreted cytokines in the tissue culture supernatants of treated cells. However, scientists often avoid immunoassays in high throughput screening (HTS) because they require cumbersome manipulations. Applied B...Caspase-3 Activation - An Indicator of Apoptosis in Image-Based Assays
The understanding of the cellular signaling processes leading to programmed cell death (apoptosis) is of utmost importance in the study of autoimmune diseases such as rheumatoid arthritis or Parkinsons and Alzheimers disease. Here, errors in the signaling cascade are believed to lead to premature apoptosis in the affected t...Amplification Efficiency of TaqMan Gene Expression Assays
Applied Biosystems has designed and manufactured over 40,000 real-time PCR assays for measuring the expression of genes in humans, mice, and rats. TaqMan Gene Expression Assays each consist of amplification primers (forward and reverse) and a fluorescent...P incorporation, which, however, involves the problems of radioactive work, safety and disposal. Eu-...
Promegas Multiplexed Cell Viability and Apoptosis Assays performed on the PHERAstar
Promega Corporation, Madison, WI, USA Application Note 138 Rev. 07/2006 Correlated measurement of an apoptosis marker (or caspase activity) and cell viability Monitoring of both lumi...Promegas Multiplexed Luciferase Reporter and Cell Viability Assays performed on the PHERAstar
Promega Corporation, Madison, WI, USA Application Note 139 Rev. 7/2006 Multiplexing homogeneous cell-based assays for analysis of different parameters from a single sample well Monito...Third Wave Achieves Milestone, Delivers 200,000 SNP Assays to RIKEN In Japan
MADISON, Wis. -- Since the beginning of 2003, Third Wave has been supplying its Invader SNP assays to Dr. Yusuke Nakamura, group directo...Other TagsOther Contentsdrowsinessdrowsinessdressingdressingdressingdrowningdrowningingestioningestioningestioningestioningestioningestionaddictionaddictionaddictionaddictionaddictionaddictiondrydrydrydrydrydrydrydrydryduodenalduodenaldyesdyesdyesdyesdyesdyes
(Date:9/4/2008)... , EVANSTON, Ill. --- Biological systems are cons...s for survival amidst environmental fluctuations a...lism, a Northwestern University research team has ...l metabolisms that are especially well suited to h...ons and mutations. , The results, published on...
(Date:9/4/2008)... , CINCINNATINew research from the University of ...ed to produce hard plasticsbisphenol A (BPA)as a r...nces. , In a laboratory study, using fresh human...s a key hormone, adiponectin, which is responsible...d puts people at a substantially higher risk for m...
(Date:9/4/2008)... , CAMBRIDGE, Mass., Sept. 4, 2008 Declaring the... and philanthropy, Los Angeles-based philanthropis...have increased their total gift to the Broad Insti... their previous gifts totaling $200 million, the B...onvert the Institute, which had been launched as a...
(Date:9/4/2008)... , PROVIDENCE New research from Rhode Island Hos...tus (T2DM) can contribute to mild neurodegeneratio...D) the first study to show that obesity can cause...rnal of Alzheimer,s Disease Volume 15:1 (Septembe...esearcher Suzanne de la Monte, MD, MPH, of Rhode I...
Breaking Biology News(10 mins):New evidence on the robustness of metabolic networks 2Bisphenol A linked to metabolic syndrome in human tissue 2Eli and Edythe L. Broad make unprecedented gift to endow Broad Institute of Harvard & MIT 2Eli and Edythe L. Broad make unprecedented gift to endow Broad Institute of Harvard & MIT 3Eli and Edythe L. Broad make unprecedented gift to endow Broad Institute of Harvard & MIT 4Eli and Edythe L. Broad make unprecedented gift to endow Broad Institute of Harvard & MIT 5BARACLUDE data show low resistance over 5 years in nucleoside naive hepatitis B patients 2595 1BARACLUDE data show low resistance over 5 years in nucleoside naive hepatitis B patients 2595 2BARACLUDE data show low resistance over 5 years in nucleoside naive hepatitis B patients 2595 3Patients Do Better at Hospitals With Clinical Trials 14992 1Patients Do Better at Hospitals With Clinical Trials 14992 2Derma Sciences to Report 2007 Fourth Quarter and Year End Results on April 1 2008 14990 1Derma Sciences to Report 2007 Fourth Quarter and Year End Results on April 1 2008 14990 2Three Out of Four Pennsylvania Voters Support Increased State Funding for Home Healthcare Services 14988 1Three Out of Four Pennsylvania Voters Support Increased State Funding for Home Healthcare Services 14988 2Three Out of Four Pennsylvania Voters Support Increased State Funding for Home Healthcare Services 14988 3[0] New evidence on the robustness of metabolic networks[0] Bisphenol A linked to metabolic syndrome in human tissue[0] Eli and Edythe L. Broad make unprecedented gift to endow Broad Institute of Harvard & MIT[0] Rhode Island Hospital study finds link between obesity, type 2 diabetes and neurodegeneration
(Date:9/5/2008)...s on Web site shouldn,t overreact or stop taking t... -- The U.S. Food and Drug Administration has star...for potential safety issues, the agency said Frida... it means we have begun analysis to determine whet...r evaluation," Dr. Gerald Dal Pan, director of the...
(Date:9/5/2008)...shion Week,s Annual Destination to Feature VelaSha... (PRWEB) September 5, 2008 -- http://www.american...can Laser], the largest provider of laser hair rem...he U.S., announces sponsorship of the STYLE360 sho...pt. 7-10, serves as the alternative Fashion Week d...
(Date:9/4/2008)...ivate Medicare Advantage (MA) plans will be paid a...ompared to what the same enrollee would have cost ...m, according to a new report from The Commonwealth... mandated by the Medicare Improvements for Patient...take effect beginning in 2010 had been fully in p...
(Date:9/4/2008)...ons they say could help doctors evaluate risks , ...accine safety experts say that almost all kids who... with close monitoring and a set of standard preca... Pediatrics , a team of experts led by the Johns H...set of instructions -- an algorithm -- to help phy...
Breaking Medicine News(10 mins):Health News:FDA to List Drugs Under Review for Safety Issues 2Health News:American Laser Centers Sponsors STYLE360 to Share the Skinny on Body Shaping 2Health News:Extra payments to Medicare Advantage plans to total $8.5 billion in 2008 2Health News:Even Kids With Known Allergies Can Be Safely Vaccinated 2| Copyright © 2003-2008 Bio-Medicine. All rights reserved. ABOUT | CONTACT US | DISCLAIMER | PRIVACY POLICY | TERMS AND CONDITIONS |  |