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Antibodies for Studying NMDA Receptor Protein Expression and Synapse-Specific Immunolabeling

Danny Q. Hoang Michael Browning Xinping Yang PeterPingerelli Stratagene Cloning Systems, Inc. Stratagene introduces affinity purified antibodies for NMDA receptorsubunits that are useful for studying expression of the NR2A, NR2B and NR2Csubunit proteins...

Simple, Sensitive Isotyping of Mouse Monoclonal Antibodies

Weiping Yang Matt C. Petre Darrel Holmes Peter L.Pingerelli Stratagene Cloning Systems, Inc. Stratagenes IsoDetect mouse monoclonal antibody isotyping kit is asimple, rapid and sensitive method for identifying mouse monoclonal antibodyisotypes in a single step. Each IsoDetect test card...

Mx4000 Multiplex Quantitative PCR System*,PPP

Stratagene has improved upon currently available QPCR machines with the Mx4000 multiplex quantitative PCR system. This new system offers better performance and functionality at a more economical price. Real-time quantitative PCR (QPCR) allows researchers to quickly and easilyquantify nuclei...

Quantification made easy

With the aid of spectroscopy, the quantitative analysis of nucleic acids and proteins has established itself as a routine method in many laboratories. It includes absorption measurements in the ultraviolet and in the visibility range. Proteins are measured (directly) at 280 nm, nucleic acids at 260 nm and colorimetric...

High-throughput gene expression analysis to screen for anti-asthma drugs

Andreas Pahl, Uwe Oelmller,* and Susanne Ullmann Department of Pharmacology, University of Erlangen, Erlangen, Germany * QIAGEN GmbH, Hilden Germany Drug library screening based on gene expression analysis requires reliable and reproducible methods of RNA pu...

QuantiTect SYBR Green RT-PCR Kit

In one-step quantification of RNA using the QuantiTect SYBR Green RT-PCR Kit, sensitivity is enhanced by combining HotStarTaq DNA Polymerase with a unique blend of Sensiscript and Omniscript Reverse Transcriptases. Sensiscript RT is designed for use with very small amounts of RNA, while Omnis...

QuantiTect Probe PCR Kit

For quantitative, real-time PCR and two-step RT-PCR using sequence-specific probes Component Included Concentration QuantiTect Probe PCR Master Mix...

Quantitation of Cabergoline at Extremely Low Plasma Concentrations with a Triple Quadrupole Mass Spectrometer

Nicola Hughes, Jean-Jacques Dunyach, 1Biovail Contract Research,Toronto,Ontario,Canada; 2Thermo Finnigan,San Jose,CA,USA; 3Therm...

Improved Quantitative Selectivity of Clenbuterol in Human Urine using High Resolution on the TSQ Quantum Mass Spectrometer

Mark Churchill , John Henninge, 1 Thermo Finnigan, Stafford House, 1 Boundary Park, Boundary Way, Hemel Hempstead, HP2 7GE, UK; 2 Aker University Hospital, Hormone Laboratory, Section for Doping Analysis,Trondheimsv...

Quantification of the Purinergic Receptor P2X3 using ICAT and Orthogonal 2D LC-MS/MS with an Ion Trap Mass Spectrometer

David Barnidge , Gargi Choudhary Neuromics, Inc., Minneapolis, MN Thermo Finnigan, San Jose, CA The data presented here was acquired using the Proteome...

Bioanalytical Method Intraday Validation for the Quantitation of Paroxetine in Bovine Plasma using the TSQ Quantum Mass Spectrometer

Mark Churchill and Mark Harrison, Thermo Finnigan, Hemel Hempstead, UK The data presented here can be acquired using a Thermo Finnigan TSQ Quantum mass spectrometer...

The LightCycler is the only System for Quantitative PCR that is as Flexible as You are!

The LightCycler is the only System for Quantitative PCR that is as Flexible as You are! Choose the LightCycler PCR System, the only instrument flexible enough to meet your changing research needs. Central to the LightCycler System is the LightCycler Instrument which is much more than "Just an...

Comparison of Different Methods of mRNA Quantitation

<TABLE width="96%" border=0 align="left" cellPadding=8 cellSpacing=1> Nuclease Protection Assays <b...

Detection of Mutations in the Fas Antigen of Lymphoma Tumors by RT-PCR and Denaturing Gradient Gel Electrophoresis

Terry H. Landowski, Ibrahim Buyuksal, and William S. DaltonH. Lee Moffitt Cancer Center and Research Institute, University of SouthFlorida, 12902 Magnolia Drive, Tampa FL 33612 Introduction Identifying of mutations within specific genes has become an important strategy in determining the diagnosis and prognosis f...

Using the OligoGreen Oligonucleotide Quantitation Reagent in the Gemini XS, Gemini EM and SpectraMax M2 Microplate Readers (MaxLine Application Note #21)

By Yan Zhang., Molecular Devices Corporation, 1311 Orleans Dr., Sunnyvale, CA 94089. This application note describes how to use the OliGreen Pro software from...

ATP Quantitation in the LMax Microplate Luminometer (MaxLine Application Note #40)

Evelyn McGown, Ph.D. and Michael Su, M.S. Molecular Devices Corporation, 8/00 Adenosine triphosphate (ATP) is present in all living cells. Because the level isstrictly-controlled, assay of ATP can be used as a indicator of viable cell number.ATP measurements are used to monitor raw materials and manufacturing...

DNA Quantitation in the LMax Microplate Luminometer (MaxLine Application Note #37)

Evelyn McGown, Ph.D. and Michael Su, M.S. Molecular Devices Corporation, 8/00 DNA measurements are important for many aspects of molecular biology. Of thespectrophotometric methods available, UV absorbance measurement at 260 nm isthe simplest and has recently gained widespread popularity in the microplateforma...

Dual-Luciferase Quantitation in the LMax Microplate Luminometer (MaxLine Application Note #39)

Evelyn McGown, Ph.D. and Michael Su, M.S. Molecular Devices Corporation, 8/00 Reporter gene assays are used to study eukaryotic gene expression. Dual geneticreporters are commonly used in transient transfections of cultured cells tominimize experimental variability caused by differences in cell number, viabili...

Solinus updates MailFoundry anti-spam hardware, switching to Linux

The MailFoundry 2100 is about three times as fast as the MailFoundry 1100, according to sales exec...

Quantification of siRNA Silencing Efficiency Using the LightCycler System

Since the first description of RNA interference (RNAi) asa protection mechanism against invasion of foreign genesappeared a few years ago, RNAi has become a powerfultool for analyzing gene function in mammalian cells. RNAiis mediated by short double-stranded RNAs that a...

Gene-Expression Analysis of TP, DPD, and TS Using the LightCycler mRNA Quantification KitsPLUS

In human cells, the enzymes thymidin phosphorylase(TP), dihydropyrimidine dehydrogenase (DPD), and thymidylatesynthase (TS) play a pivotal role in the metabolismof pyrimidines. In cancer disease medical care, thecytotoxic compound 5-fluorouracil (5-FU) is the standard...

Quantitative Measurement of Cell Proliferation Using the BrdU ELISA: A Comparison Between Colorimetric and Chemiluminescent Detection

Quantitative determination of DNA synthesis in cellcultures is now a routine procedure in many laboratories.Protocols are available for various applications,especially in cell culture systems. The effects of growthfactors, inhibition of cell division by exogenous factor...

Quantitation of the Protein MIA as a Marker for Chondrocytes in Research Samples

MIA (Melanoma Inhibitory Activity) was cloned as asecreted protein from human melanoma cell lines [1]. MIAis expressed in and secreted from malignant melanomasand chondrocytes [2, 3]. As MIA expression in chondrocytesis dependent on the differentiation status of the cel...

Quantification of Nucleosomes in Serum by the Cell Death Detection ELISAplus

Cell death as a counterpart to cell proliferation plays anessential role in the homeostasis of cell numbers in adultorganisms, furthermore in eliminating cells damaged byirradiation, chemotherapeutic drugs, hyperthermia andother influences [1-3]. There are two distinct...

Detection of antibody-stained cell surface and intracellular protein targets with the Agilent 2100 bioanalyzer

This Application Note describes how the Agilent 2100 bioanalyzer andthe Cell Fluorescence LabChip kit can be used to detect cell surfaceand intracellular protein targets by antibody staining. CD3 staining onJurkat cells as well as CD4 staining on CCRF-CEM cells and intrace...

Comparison of different protein quantitation methods

This application note compares the accuracy and reproducibility ofabsolute protein quantitation of the Agilent 2100 bioanalyzer in combinationwith the Protein 200 Plus assay to the batch-based methodsLowry and Bradford as well as to denaturing gel-elctrophoresis (SDSPAGE)....

Quantitative end-point RT-PCR gene expression measurement using the Agilent 2100 Bioanalyzer and standardized RT-PCR

This application note describes how the Agilent 2100 bioanalyzer canbe used in combination with Standardized RT (StaRT)-PCR reagentsfrom Gene Express National Enterprises (G.E.N.E.), Inc. (Huntsville,AL, USA) to obtain highly reproducible, standardized gene expressionme...

Quality control of antibodies using the 2100 bioanalyzer and the Protein 200 Plus assay

Antibodies are commonly used in diagnostics, as research tools(for example protein arrays) or as biopharmaceutical therapeutics. Inall cases the successful production of antibodies requires precise testingand quality control. The key quality criteria include: protein ident...

Detection of Genetically Modified Soybean in Processed Foods Using Real-Time Quantitative PCR with SYBR Green I Dye on the DNA Engine Opticon 2 System

MJ Research, Inc., South San Francisco, CA We present a real-time quantitative PCR (qPCR) protocol for the detection and quantitation of soy genetically modified to be resistant to the herbicide glyphosate. The assay utilizes SYBR Green...

Qualitative and Quantitative Assessment of Plasmid DNA Isolation, Rev A

Audra Day, Colette Schneider, Lisa Ottmers, and Brandt L. SchneiderDepartment of Cell Biology and Biochemistry, Texas Tech University HealthSciences Center, Lubbock, TX 79430, USA. Small-scale isolation and purification of plasmid DNA from E. coli bacterial strains is a widely used and essential techni...

Quantitation of DNA for Automated Sequencing Using the VersaFluor Fluorometer

Wenda Ribeiro and Margaret Saha, Department of Biology, College of William and Mary, Williamsburg, Virginia 23187 DNA sequence analysis is an integral part of genetic research. Recently, the use of automated sequencers has become increasingly more common in small research laboratory sett...

Quantitative Analysis of Protein-DNA Associations in Vivo Using Real-Time PCR, Rev A

Swarup K Chakrabarti and Raghavendra G Mirmira*, Departments of Medicine and Pharmacology, Division of Endocrinology and Metabolism, University of Virginia Health Sciences Center, 450 Ray C Hunt Dr, Box 801407, Charlottesville, VA 22903 USA *Corresponding author The chr...

Real-Time Quantification of Genomic DNA Using DyNAzyme II DNA Polymerase and SYBR Green I Dye

Mimi Amutan and David Batey, Ph.D. Real-time quantification was performed for lambda and human genomic DNA on the DNA Engine Opticon system (MJ Research). DyNAzyme II DNA polymerase*was used for amplification, and SYBR Green I fluorescent dye was used for detection. The sensitivity and linear dynamic range of...

Quantitation of RNA for Hybridization Blots Using the VersaFluor Fluorometer

Matthew W. Fields, Section of Microbiology, Cornell University, Ithaca,New York 14853 Biologists frequently monitor the effect of environmental stimuli on living organisms by quantitating mRNA levels. The relative abundance of mRNA is typically determined with probes and hybridization blots, b...

Development of a Multiplex Bead-Based Assay for Antibody Screening of a Nonhuman Primate Colony on the Bio-Plex System

LaRene Kuller, David Anderson, Dominic Andrada, and Richard Grant, NationalPrimate Research Center, University of Washington, Seattle, WA 98195 USA We have been using four separate enzyme-linked immunosorbent assays (ELISAs) to screen our primate colony for the viruses of greatest concern: simian...

Quantitation of Lymphangiogenesis Using the iCycler iQ Real-Time PCR Detection System and Scorpions Detection System, Rev A

Giles Cunnick and Wen Guo Jiang, Metastasis Research Group, UniversityDepartment of Surgery, University of Wales College of Medicine, Cardiff, UK Lymphangiogenesis refers to the formation of new lymphatic vessels. This process may occur in normal developing tissues or in tumors. In addition to sprea...

Rapid, Reproducible Real-Time Quantitative RT-PCR Using the iCycler iQ Real-Time PCR Detection System and iQ Supermix, Rev A

Departments of Ophthalmology and Cell Biology, Duke University Medical Center, Durham, NC 27710 USA Correspondence: Catherine Bowes Rickman, PhD, Department of Ophthalmology, Duke University Medical Center, Box 3802, Durham, NC 27710, Phone (91...

Quantitation of GAD67 Gene Expression in Prefrontal Cortex of Schizophrenia Patients Using the iCycler iQ Detection System and Molecular Beacons, Rev A

130 West Kingsbridge Road, Bronx, NY 10468 USA Schizophrenia is a severe mental illness that afflicts about 1% of the population (Strange 1992). Patients experience a variety of symptoms that have been divided into subgroups...

Using the VersaFluor Fluorometer to Quantitate GUS Expression in Plant Tissues Bombarded With the Biolistic PDS-1000/He System

Nicholas N. Lyssenko and Mark L. Tucker, Soybean and Alfalfa Research Lab,USDA, ARS, Bldg. 006, BARC-West, Beltsville, MD, USA 20705 In 1987 Jefferson et al. described the use of β-glucuronidase (GUS) as a marker to study gene expression in plants. Expression of GUS activity in plant tissu...

Benchmark Plus Microplate Reader: Quantitation of Protein Concentration Using Two Different Colorimetric Assay Kits, Rev A

Theresa Redila-Flores, Bio-Rad Laboratories, 2000 Alfred Nobel Drive,Hercules, CA 94547 USA The Bradford and Lowry assays are 2 colorimetric assays that are commonly used to quantitate protein concentration. Bio-Rads protein assay (catalog #500-0001), based on the Bradford dye-binding assay (Bradford 1976), measures t...