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Amplification in Biological Technology

World Gene Amplification Technologies Market to Reach $2.2 Billion by 2015, According to New Report by Global Industry Analysts, Inc.

Gene Amplification technology is one of the rapidly growing markets in life science field. By the year 2015, global market for Gene Amplification technologies is projected to reach $2.2 billion. Major factors that continue to affect the industry are patent expiry, and competitive advantages assoc...

Sigma-Aldrich Releases WTA2, a Transplex Whole Transcriptome Amplification Technology for Degraded RNA Samples

WTA2 Kits Offer Rapid, Robust Amplification of Partially Degraded RNA ST. LOUIS, Oct. 28 /PRNewswire-FirstCall/ -- Sigma-Aldrich (Nasdaq: SIAL) today announced the launch of its TransPlex(R) Whole Transcriptome Amplification (WTA2) technology ( http://www.sigma.com/wta ). TransPlex technology...

Powersafe Technology Corp. Recapitalizes, Executes Merger Agreement with Amplification Technologies, and Invests in Amplification

VALLEY STREAM, N.Y., April 11 /PRNewswire-FirstCall/ -- Powersafe Technology Corp. (OTC Bulletin Board: "PWSF") has entered into a merger agreement with Amplification Technologies, Inc., a privately held Delaware corporation. Pursuant to the terms of the merger agreement, Amplification will me...

Biomatrica's PCRboost(TM) Enables 5-Fold Improvement in Amplification of Difficult Samples

SAN DIEGO, March 12 /PRNewswire/ -- Biomatrica, the leader in room temperature biological sample stabilization, announced the introduction of PCRboost, a reagent that enhances PCR and RT-PCR performance 5-fold or more by improving sensitivity and specificity during amplification of genomic DNA...

Genomic Analysis of Formalin-Fixed Paraffin Embedded (FFPE) Tissues through the use of Whole Genome Amplification (WGA)

The past is a source of knowledge, and the future is a source of hope. S. Ambrose (1936-2002) Ernest Mueller Preserved samples from medical, forensic, museum and other archival collections represent a rich source of study material, much of it meticulously collected...

Scalable Automation of the TempliPhi DNA Sequencing Template Amplification and DYEnamic ET Terminator Sequencing Kits on the Sciclone ALH 3000

Geoffrey Grove, Ed Alderman Applied Science and Technology, Caliper Life Sciences, Inc., Hopkinton, MA 01748 Monica Adams, Jason Brennan Development Integration, Amersham BioSciences, 800 Centennial Avenue, Piscataway, NJ 08855 Overview The TempliPhi DN...

GenomiPhi amplification of FFPE tissue DNA for array CGH

Key words: formalin-fixed paraffin-embedded (FFPE) • GenomiPhi • whole-genome amplification (WGA) • degenerate oligonucleotideprimed PCR (DOP-PCR) • Cy3/Cy5-dCTP probe labeling • comparative genomic hybridization (CGH) • bacterial...

Automation of TempliPhi and GenomiPhi DNA Amplification Kits on the Genesis Freedom Robotic Sample Processor

M. Adams and R. Deadman GE Healthcare, Piscataway, NJ, US GE Healthcare offers a range of DNA amplification products to meet all genomic DNA and sequencing template preparation needs. A combination of these kits with the Genesis Freedom™...

GenomiPhi: protocol for BAC amplification and fabrication of slides for array CGH

Prasad D.K. Dhulipala 1 , Rao S. Takkellapati 1 , Kate Lavrenov 1 , Scott Hamilton 1 , and Jessica Pole 2 1 GE Healthcare, Piscataway, NJ, USA 2 Hutchison/MRC Research Centre, University of Cambridge, Cambridge, UK ...

Oragene and Whole Genome Amplification with GenomiPhi

J. Chartier DNA Genotek Inc., Ottawa, ON, Canada DNA collected with Oragene is successfully amplified using the GenomiPhi DNA Amplification Kit and has the potential to generate near-unlimited quantities of DNA for downstream experi...

RNArticles: RNA Amplification Used to Develop a New In Vitro HCMV Assay

Gene arrays can be used to simultaneously measure the expression levels of thousands of genes in a single experiment. One limitation of this technology is that large amounts of RNA may be required for each array hybridization experiment -- typ...

RNA Amplification Just Got Better: MessageAmp II

Compatible with GeneChip analysis Amplify as little as 100 ng RNA in a single round Includes the new ArrayScript engineered M-MLV RT, engineered to maximize ...

RNA Amplification for Array Analysis

Frequently Asked Questions A. How is fold amplification calculated? RNA amplification using the Van Gelder and Eberwine technique (Van Gelder 1990) utilizes an oligo(dT) primer containing the T7 RNA p...

Is RNA Amplification Necessary for Microarrays?

RNA amplification using the Van Gelder and Eberwine technique (1) has been used extensively for target synthesis and labeling in array analysis experiments. Amplification of RNA is clearly necessary when workin...

Automated RNA Amplification for Arrays

Karen Rose, Sonal Patel, and David Quilter (GeneXP Biosciences Inc.) Efficient and cost-effective method for synthesizing aRNA in a high throughput format Increase throughput...

Protocol for GenomePlex Whole Genome Amplification from Formalin-Fixed Parrafin-Embedded (FFPE) tissue

Application Guide ........................................................................................................................2 I. Description ......................................................................................

Protocol for GenomePlex Whole Genome Amplification from Whole Blood

Application Guide I. Description II. Product Components III. Materials to be Supplied by the User IV. Protocol for Extraction of DNA from Whole Blood V. Protocol for GenomePlex Whole Genome Amplification from Whole Blood VI. Quantification of Amplified...

Protocol for GenomePlex Whole Genome Amplification from Soil

Application Guide I. Description II. Product Components III. Materials to be Supplied by the User IV. Protocol for Extraction of DNA from Soil V. Protocol for GenomePlex Whole Genome Amplification from Soil VI. Quantification of Amplified products VII. ...

Protocol for GenomePlex Whole Genome Amplification from Plant

Application Guide I. Description II. Product Components III. Materials to be Supplied by the User IV. Protocol for Extraction of DNA from Plant V. Protocol for GenomePlex Whole Genome Amplification from Plant VI. Quantification of Amplified Products ...

Protocol for GenomePlex Whole Genome Amplification from Blood Card

Application Guide I. Description II. Product Components III. Materials to be Supplied by the User IV. Protocol for Extraction of DNA from Blood Card V. Protocol for GenomePlex Whole Genome Amplification from Blood Card VI. Quantification of Amplified Pro...

Protocol for GenomePlex Whole Genome Amplification from Buccal Swab

Application Guide I. Description II. Product Components III. Materials to be Supplied by the User IV. Protocol for Extraction of DNA from Buccal Swab V. Protocol for GenomePlex Whole Genome Amplification from Buccal Swab VI. Quantification of Amplified P...

Efficient and Reliable Linear Amplification of cRNA

Gene Expression Profiling Using Microarray Kits from Roche Applied Science The use of gene-expression profiling, a genomic application measuring mRNA transcript levels of many gen...

Advantages of Roche Applied Science amplification products

The Polymerase Chain Reaction invented by Kary Mullis in 1983 drastically changed the course of modern life science research by opening an enormous variety of new opportunities to study and understand the crucial role of nucle...

Successful PCR amplification and subcloning of a GC-rich DNA fragment

Petra Majlingov, Paolo Paroncini, Silvia Svegliati, Michele Luchetti, and Armando Gabrielli Laboratorio di Medicina Molecolare, Istituto di Clinica Medica, Polo Didattico Scientifico, Ancona, Italy The formati...

Using the TripleMaster PCR System for Robust Amplification of GC-Rich DNA Templates

Scott Tarpinian Eppendorf 5 Prime, Boulder, CO, USA Introduction The polymerase chain reaction (PCR*) is a powerful and sensitive technique used by scientists to amplify large quant...

Optimizing the Direct Amplification of Missing cDNA 5' Ends Using the Eppendorf Mastercycler gradient

Optimizing the Direct Amplification of Missing cDNA 5' Ends Using the Eppendorf Mastercycler gradient Ulrich Genschel Institute of General Botany, University of Hamburg Christian Rohrer, Eppendorf AG, Hamburg ...

Optimizing DNA Amplification Protocols using the Eppendorf Mastercycler

Peter Scheinert, PhD, Barbara Behrens, PhD, and Dietmar Kahle, PhD Peter Scheinert, PhD, Bernhard-Nocht-Institute for Tropical Medicine, Virology Dept., Bernhard-Nocht-Str. 74, D-20359 Hamburg former address: Universi...

Optimizing the Direct Amplification of Missing cDNA 5' Ends Using the Eppendorf Mastercycler gradient

Optimizing the Direct Amplification of Missing cDNA 5' Ends Using the Eppendorf Mastercycler gradient Ulrich Genschel Institute of General Botany, University of Hamburg Christian Rohrer, Eppendorf AG, Hamburg ...

Improve Amplification Specificity with Hot Start PCR Enzyme

SureStart Taq DNA polymerase utilizes ultra-pure Taq2000 DNA polymerase SureStart Taq DNA polymerase features a modified version of Taq2000 DNA polymerase with hot start capability. Using SureStart Taq, hot start is in- corporated into PCR protocols previ...

Optimizing pfuturbo DNA Polymerase Amplification Reactions with Perfect Match PCR Enhancer

Improve PCR product yield and specificity for difficult amplifications Optimizing pfuturbo DNA Polymerase Amplification Reactions with Perfect Match PCR Enhancer Frances Bai Holly Hogrefe Stratagene Certain PCR amplification systems produce multiple product bands or ...

Enhanced Amplification of Long Targets with PfuTurbo DNA Polymerase

High-fidelity PCR of genomic targets up to 19 kb Enhanced Amplification of Long Targets with PfuTurbo DNA Polymerase Michael Borns Holly Hogrefe Stratagene Improve amplification of long targets with PfuTurbo DNA polymerase* by making simple modifications to PCR reacti...

Greater Amplification Specificity with New Hot Start PCR Enzyme

SureStart Taq DNA polymerase makes hot start simple and efficient Jonathan Eads Frances Bai Ronda Allen Holly Hogrefe ...

High Throughput aRNA Amplification

MessageAmp II-96 aRNA Amplification Kit Amplify as little as 100 ng of input RNA in a single round for GeneChip analysis Includes ArrayScript, an engineered M-MLV RT, to maximize yields of full-length cDNA ...

Improving RNA Amplification

Performance of New MessageAmp II on High Density Oligonucleotide Microarrays New ArrayScript, an M-MLV RT engineered to maximize yields of full-length cDNA Amplify as little as 1...

Better RNA Amplification

NEW Amino Allyl MessageAmp II Kit now with Cy Dyes included! * Amplify as little as 100 ng of input RNA in a single round for GeneChip analysis Greater sensitivity due to higher yields of full-length cDNA aRN...

Gene Expression Arrays: Highly Sensitive Detection of Expression Patterns with Improved Tools for Target Amplification

Introduction As the human genome project is completed, microarray technology offers the potential to study the genomes complexity. This technology facilitates the direct ext...

prostar RT-PCR Systems for Robust High-Fidelity RNA Amplification

Powerful high-fidelity RT-PCR systems in two convenient formats Michael Borns Janice Cline Kris Connors Ronda Allen Holly Hogrefe Stratagene Stratagenes prostar RT-PCR systems are powerful systems that permit efficient, high-fidelity amplification of RNA in t...

Cepheid Schedules 2009 Second Quarter Financial Results Announcement and Webcast

...aboratory procedures. The company's easy-to-use systems integrate a number of complicated and time-intensive steps, including sample preparation, DNA amplification and detection, which enable the analysis of complex biological samples in its proprietary test cartridges. Through its strong molecular biology capab...

New England Journal of Medicine Study Highlights Need for Rapid, PCR-based Group B Streptococcus (GBS) Testing at Time of Admission for Labor and Delivery

... on-board sample preparation with real-time PCR (polymerase chain reaction) amplification and detection functions for fully integrated and automated nucleic acid ana... of complicated and time-intensive steps, including sample preparation, DNA amplification and detection, which enable the analysis of complex biological samples in i...

Prescription-Free Digital Hearing Aid Just Released

...ving the needs of hearing impaired people. HearPod aids are 100% digital, full-featured and have some of the most advanced noise canceling and sound amplification computer chips available. Their innovative design also reduces sound feedback inside the ear, thereby making HearPod hearing aids among the most comfo...
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