Amplification

prostar RT-PCR Systems for Robust High-Fidelity RNA Amplification

Michael Borns Janice Cline Kris Connors RondaAllen Holly Hogrefe Stratagene RT-PCR systems are powerfulsystems that permit efficient, high-fidelity amplification of RNA in twoconvenient formats. The ProSTAR Ultra HF RT-PCR...

Simple Purification of DNA from Plasmid Minipreps, PCR Amplifications and Agarose Gels

High yields of extremely pure DNA with StrataPrep nucleic acidpurification kits Stratagene Cloning Systems, Inc. Stratagene announces a new line of DNA purification products, whichprovide reliable isolation of high-purity miniprep plasmid, PCR product andgel-fractionate...

Greater Amplification Specificity with New Hot Start PCR Enzyme

Taq DNA polymerase makes hot start simple and efficient Jonathan Eads Frances Bai Ronda Allen Holly Hogrefe <p align="...

Enhanced Amplification of Long Targets with PfuTurbo DNA Polymerase

DNAPolymerase Stratagene DNA polymerase* by making simple modifications to PCR reactionconditions. In this update, we d...

Optimizing pfuturbo DNA Polymerase Amplification Reactions with Perfect Match PCR Enhancer

Optimizing pfuturbo DNA Polymerase Amplification Reactions withPerfect Match Stratagene Certain PCR amplification systems produce multiple pro...

Improve Amplification Specificity with Hot Start PCR Enzyme

Taq2000 features a modified version of Taq2000 with hot start capability. Using SureStart Taq, hot start is in- corporated into PCR protocols previously optimized with Taq DNA...

Amplification of the amoA Gene of Nitrosococcus oceani Bacteria Using Eppendorf MasterTaq

University of Georgia, Department of Marine Sciences This application describes the use of the MasterTaq polymerase kit for improving PCR efficiency when studying functio...

Amplification of Mouse cDNAs for Microarrays Using the Eppendorf MasterTaq Kit

BIOGEM, Division of Biology, University of California San Diego, La Jolla, CA 92093-0349 e-mail: ghardiman@ucsd.edu www.biogem.ucsd.edu Microarray technology, primarily advanced by Pat...

Optimizing the Direct Amplification of Missing cDNA 5' Ends Using the Eppendorf Mastercycler gradient

Using the Eppendorf Mastercycler gradient Institute of General Botany, University of Hamburg Christian Rohrer, Eppendorf AG, Hamburg In man...

Optimizing DNA Amplification Protocols using the Eppendorf Mastercycler

Peter Scheinert, PhD, Barbara Behrens, PhD, and Dietmar Kahle, PhD Peter Scheinert, PhD, Bernhard-Nocht-Institute for Tropical Medicine, Virology Dept., Bernhard-Nocht-Str. 74, D-20359 Hamburg former address: University of Kiel, Institute for General Microbiology, Am Botanischen Garten, D-24118 K...

Amplification of Epstein-Barr Virus Exon C with Difficult Primers Using Eppendorf Mastercycler gradient

Using Eppendorf Mastercycler gradient NPC Laboratory, Institute of Postgraduate Studies and Research, University of Malaya, Kuala Lumpur, Malaysia...

Optimizing the Direct Amplification of Missing cDNA 5' Ends Using the Eppendorf Mastercycler gradient

Using the Eppendorf Mastercycler gradient Institute of General Botany, University of Hamburg Christian Rohrer, Eppendorf AG, Hamburg In many research projects, cD...

The TripleMaster PCR System for three different amplifications

Christian Rohrer, Eppendorf AG Standard PCR components (Taq polymerase and reaction buffers) are used for the general amplification of relatively short DNA fragments (100 bp to roughly 2,000 bp). However, demanding...

Using the TripleMaster PCR System for Robust Amplification of GC-Rich DNA Templates

Eppendorf 5 Prime, Boulder, CO, USA The polymerase chain reaction (PCR*) is a powerful and sensitive technique used by scientists to amplify large quantities of DNA. Although the reaction mix and thermal cycling conditi...

Successful PCR amplification and subcloning of a GC-rich DNA fragment

Petra Majlingov, Paolo Paroncini, Silvia Svegliati, Michele Luchetti, and Armando Gabrielli Laboratorio di Medicina Molecolare, Istituto di Clinica Medica, Polo Didattico Scientifico, Ancona, Italy The formation of robust secondary structures that resist denaturation makes GC-rich sequ...

Advantages of Roche Applied Science amplification products

The Polymerase Chain Reaction invented by Kary Mullis in 1983 drastically changed the course of modern life science research by opening an enormous variety of new opportunities to study and understand the crucial role of nucleic acids in living organisms. Roche Applied Science recognized ea...

RNArticles: RNA Amplification Used to Develop a New In Vitro HCMV Assay

Gene arrays can be used to simultaneously measure the expression levels of thousands of genes in a single experiment. One limitation of this technology is that large amounts of RNA may be required for each array hybridization experiment -- typically 10 g of total RNA. This becomes problematic when working with limited sampl...

Scalable Automation of the TempliPhi DNA Sequencing Template Amplification and DYEnamic ET Terminator Sequencing Kits on the Sciclone ALH 3000

The TempliPhi DNA Sequencing Template Amplification Kit is designed to eliminate...

Gene Expression Arrays: Highly Sensitive Detection of Expression Patterns with Improved Tools for Target Amplification

As the human genome project is completed, microarray technology offers the potential to study the genomes complexity. This technology facilitates the direct extraction of functional information from nucleic a...

Efficient and Reliable Linear Amplification of cRNA

Gene Expression Profiling Using Microarray Kits from Roche Applied Science The use of gene-expression profiling, a genomic applicationmeasuring mRNA transcript levels of many genes inparallel, has been growing rapidly...

Protocol for GenomePlex Whole Genome Amplification from Buccal Swab

Application Guide I. II. III. IV. V. VI. <a href="#VI"...

Protocol for GenomePlex Whole Genome Amplification from Blood Card

Application Guide I. II. III. IV. V. VI. Quantificat...

Protocol for GenomePlex Whole Genome Amplification from Plant

Application Guide I. II. III. IV. V. VI. Quantification of Amp...

Protocol for GenomePlex Whole Genome Amplification from Soil

Application Guide I. II. III. IV. V. VI. Quantification of Amplifie...

Protocol for GenomePlex Whole Genome Amplification from Whole Blood

Application Guide I. II. III. IV. V. VI....

Protocol for GenomePlex Whole Genome Amplification from Formalin-Fixed Parrafin-Embedded (FFPE) tissue

I. Desc...

Automated RNA Amplification for Arrays

Karen Rose, Sonal Patel, and David Quilter (GeneXP Biosciences Inc.) Efficient and cost-effective method for synthesizing aRNA in a high throughput format Rapid, reliable, and consiste...

Better RNA Amplification

NEW Amino Allyl MessageAmp II Kit now with Cy Dyes included! * Amplify as little as 100 ng of input RNA in a single round for GeneChip analysis aRNA synthesis powered by MEGAscript, the best technology for in...

Improving RNA Amplification

Performance of New MessageAmp II on High Density Oligonucleotide Microarrays New ArrayScript, an M-MLV RT engineered to maximize yields of full-length cDNA Amplify as little as 100 ng of input RNA in a single round for GeneChip analysis</...

Is RNA Amplification Necessary for Microarrays?

RNA amplification using the Van Gelder and Eberwine technique (1) has been used extensively for target synthesis and labeling in array analysis experiments. Amplification of RNA is clearly necessary when working with limited samples (needle biopsies, LCM samples, etc.), but is...

High Throughput aRNA Amplification

Amplify as little as 100 ng of input RNA in a single round for GeneChip analysis Includes ArrayScript, an engineered M-MLV RT, to maximize yields of full-length cDNA aRNA synthesis powered by MEGAscript High Y...

RNA Amplification for Array Analysis

A. How is fold amplification calculated? RNA amplification using the Van Gelder and Eberwine technique (Van Gelder 1990) utilizes an oligo(dT) primer containing the T7 RNA polymerase promoter for synthesis of first strand cDNA. The po...

RNA Amplification Just Got Better: MessageAmp II

Compatible with GeneChip analysis Amplify as little as 100 ng RNA in a single round Includes the new ArrayScript engineered M-MLV RT, engineered to maximize yields of full- length cDNA cRNA synt...

Oragene and Whole Genome Amplification with GenomiPhi

DNA Genotek Inc., Ottawa, ON, Canada DNA collected with Oragene is successfully amplified using the GenomiPhi DNA Amplification Kit and has the potential to generate near-unlimited quantities of DNA for downstream experiments. As the genotyping of...

GenomiPhi: protocol for BAC amplification and fabrication of slides for array CGH

Prasad D.K. Dhulipala GE Healthcare, Piscataway, NJ, USA 2 Hutchison/MRC Research Centre, University of Cambridge, Cambridge, UK We present a compara...

Automation of TempliPhi and GenomiPhi DNA Amplification Kits on the Genesis Freedom Robotic Sample Processor

M. Adams and R. Deadman GE Healthcare, Piscataway, NJ, US GE Healthcare offers a range of DNA amplification products to meet all genomic DNA and sequencing template preparation needs. A combination of these kits with the Genesis Freedom™ Robotic Sample Processor (RSP) from Tecan (Fig 1) creates a total solution for high-throu...

GenomiPhi amplification of FFPE tissue DNA for array CGH

formalin-fixed paraffin-embedded (FFPE)• GenomiPhi • whole-genome amplification (WGA)• degenerate oligonucleotideprimed PCR (DOP-PCR)• Cy3/Cy5-dCTP probe labeling • comparativegenomic hybridization (CGH) • bacterial artificialchromosome (BAC) array • array CGH Human ge...

Amplification of genome-representative DNA from limited sources with GenomePlex WGA technology for use in genetic alterations studies

whole-genome amplification (WGA) systemis a notable achievement. </...

Amplification Efficiency of TaqMan Gene Expression Assays

Applied Biosystems has designed and manufactured over 40,000 real-time PCR assays for measuring the expression of genes in humans, mice, and rats. TaqMan Gene Expression Assays each consist of amplification primers (forward and reverse) and a fluorescent...

Genomic Analysis of Formalin-Fixed Paraffin Embedded (FFPE) Tissues through the use of Whole Genome Amplification (WGA)

S. Ambrose (1936-2002) Ernest Mueller Preserved samples from medical, forensic, museum and other archival collections representa rich source of study material, much of it meticulously collected, characterized andpreserved through many decades of work by experts in the field. Wh...