Affi

Highly Active and Removable AffinityTagged Lambda Phosphatase

Dualspecificity Lambda Phosphatase useful for dephosphorylation serine,threonine or tyrosine residues Stratagene Cloning Systems, Inc. Stratagene's recombinant Lambda Phosphatase is a dualspecificity proteinphosphatase that has been produced as a fusion to Stratagene's novel affinitytag, the calmodulin...

Madison-based TrafficCast makes national inroads as data provider

, a company that provides traffic data to a range of other map and travel services, is expanding well beyond its Madison headquarters in order to meet with the demand from i...

Double-Affinity Purification of GST-His Tagged Proteins Using Vivapure 8-to-96 Well Cobalt-Chelate Kit

Lucia Martinez and Ario de Marco EMBL Protein Expression Core Facility, Meyerhofstr. 1, D-69117, Heidelberg, Germany Immobilized Metal ion Affinity Chromatography (IMAC) is an easy and reliable technique that allows the fast separation of His-tagged proteins from total lysates. There are several commercially available resins but all of them s...

Recover High Yields of Total Nucleic Acid from Formalin-fixed, Paraffin-embedded (FFPE) Tissue

RecoverAll Total Nucleic Acid Isolation Kit For decades, FFPE tissue has been the staple of histologists and pathologists for creating tissue slides and making patient diagnoses. More than 400 million FFPE tissue samples exist for cancer studies alone, and many of...

DNA Isolation From 50 to 20 mg of Paraffin-Embedded Tissue

Catalog #732-6343 1. Place 510 mg (0.0050.010 g) of finely minced tissue in a 1.5 ml capped tube. Add 300 l xylene and incubate 5 min with constant mixing at room temperature....

A Novel Process for Gene Expression Profiling of Rat and Mouse Tissues from Formalin-Fixed Paraffin-Embedded Sections Using Microarrays

This application note describes a method for extracting and isolating total RNA from rodent tissues that have been fixed in formalin and embedded in paraffin, and the subsequent amplification of mRNA from these tissues for use in microarray experiments. We have used this methodology to demonstrate that robust and reliable methods do exist for retrieval of...

Use of ZipTip Pipette Tips for Microscale Affinity Mass Spectrometry

Affinity chromatography forms the basis of the separation and purification of a number of biological molecules. For example, antibodies may be purified based on their affinity for protein A, or a protein may be purified on the basis of its affinity for a monospecific antibody raised against it. Typically, these purifications take place in a ch...

Affinity Depletion Cartridges for Removal of Human Serum Albumin and Immunoglobulins from Human Serum

One of the major difficulties in analyzing the proteome of human serum is the dynamic range of the concentrations of the proteins present in the sample. Human serum albumin (HSA) constitutes 57-71% of total serum protein and gammaimmunoglobulin (IgG) ranges from 8-26%. Removal of these two proteins alone clears about 75% of t...

Genomic Analysis of Formalin-Fixed Paraffin Embedded (FFPE) Tissues through the use of Whole Genome Amplification (WGA)

S. Ambrose (1936-2002) Ernest Mueller Preserved samples from medical, forensic, museum and other archival collections representa rich source of study material, much of it meticulously collected, characterized andpreserved through many decades of work by experts in the field. Wh...

Schneider National exec joins TrafficCast board

At Schneider National, Schneider is responsible for new business development and prici...

TrafficCast secures $2M from Phenomenelle Angels, NEW Capital Fund

of Appleton.<...

Study affirms information technology-productivity link

The recent report titled Digital Pro...

Wisconsin court affirms damages in Innogenetics, Abbott patent case

Madison, Wis. - The The Madison-based court affirmed the damage award to , a biopharmaceutical company based in Gent, Belg...

TrafficCast secures $1 million line of credit

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TrafficCast integrates new information source

, which provides real-time traffic data aggregation and analysis to traditional and interactive media, fleet management firms,...

Traffic data firm navigates international business

Part of the reason is that it has cut its technological teeth on some of the most knotty r...

Bruker AXS parent buys affiliate company

Both firms are part of the Bruker family of comp...

Traffic.com adds Milwaukee to service cities

, the company announced Thursday. Traffic.com will provide area commuters with "actionable traffic information featuring immediate, at-a-glance displays of roadway traffic conditions including congestion levels, inc...

Madison-based TrafficCast strikes deals with City of Shanghai

SHANGHAI On day four of Gov. Jim Doyle's trade mission to China,...

Affinity Health Streamlines IT Governance

MENASHA, Wis. - Healthcare providers are continually exploring new technologies that will result in a more effective and efficient patient experience. based in Menasha, Wisconsin is strategically investing in new healthcare technologies that will improve the quality of care it offers by focusing on those initiati...