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New Mammalian Two-Hybrid System Detects Protein-Protein Interactions

Stratagene hybrid genes are used to detect protein-protein interactions via the activationof reporter gene expression. This expression occurs as a resul...

XL10-Gold Cells Supplied in New Single-Reaction Format

solopack Gold cells reduce the time and effort needed fortransformationswithout compromise! Anna Waesche Jonathan Goodwin Amy Duncan BruceJerpseth Alan Greener Stratagene strain* in an easy-to-use, single-reaction format thatyield th...

Fractionating DNA Fragments Generated by Differential Display PCR

Pfizer Central Research, Sandwich, UK. 01304 618482 Differential display PCR (DDPCR) is a widely used technique foridentifying genes differentially expressed between cell types. Application ofDDPCR to allergic disease provides a potential means for elucidating themechanisms involved in allergic...

Optimizing pfuturbo DNA Polymerase Amplification Reactions with Perfect Match PCR Enhancer

Optimizing pfuturbo DNA Polymerase Amplification Reactions withPerfect Match Stratagene Certain PCR amplification systems produce multiple pro...

Lambda DNA Extraction Protocol

This method produces good quality LambdaDNA with yields of 1520 g lgt10 DNA per 450 l of supernatant processed. PLG tubes also can be used in conjunction with other LambdaDNA purification methods. (1) Grow a 10 ml stock of suitable plating bacteria in L-broth plus Mg2...

M13/Phagemid DNA Extraction Protocol

Efficiency of the standard protocols for production of single-stranded DNA may be improved through the use of PLG. For producing M13 ssDNA, mix 2.5 ml plating bacteria (57...

Genomic DNA Extraction from Buccal Swabs Using the Perfect gDNA Blood Mini Kit

Eppendorf 5 Prime, Inc. Buccal swabs are the least invasive way of collecting gDNA from humans, and also have the added benefit of minimizing the exposure to blood-borne pathogens. Buccal sw...

Genomic DNA Extraction from Buffy Coat Using the Perfect gDNA Blood Mini Kit

Eppendorf 5 Prime, Inc. The Perfect gDNA Blood Mini Kit is an efficient way to purify genomic DNA from 200 l of whole blood as described in the product manual. In addition,...

Lambda DNA Extraction Protocol

This method produces good quality LambdaDNA with yields of 1520 g lgt10 DNA per 450 l of supernatant processed. PLG tubes also can be used in conjunction with other LambdaDNA purification methods. (1) Grow a 10 ml stock of suitable plating bacteria in L-broth plus Mg2...

Genomic DNA Extraction from Buccal Swabs Using the Perfect gDNA Blood Mini Kit

Eppendorf 5 Prime, Inc. Buccal swabs are the least invasive way of collecting gDNA from humans, and also have the added benefit of minimizing the exposure to blood-borne pathogens. Buccal sw...

M13/Phagemid DNA Extraction Protocol

Efficiency of the standard protocols for production of single-stranded DNA may be improved through the use of PLG. For producing M13 ssDNA, mix 2.5 ml plating bacteria (57...

Genomic DNA Extraction from Buffy Coat Using the Perfect gDNA Blood Mini Kit

Eppendorf 5 Prime, Inc. The Perfect gDNA Blood Mini Kit is an efficient way to purify genomic DNA from 200 l of whole blood as described in the product manual. In addition,...

RNAClean - RNA & cDNA in vitro Reaction Purification

Agencourts RNAClean kit provides a simple, flexible and highly reproducible method for purifying nucleic acid products generated in common enzymatic RT and IVT reactions such as cDNA and cRNA. The kit is formatted for microarray gene expression analysis experiments and is easily performed in manual or automated formats. RNAClean eliminates...

Fractionation of Bovicola ovis Homogenates Using the Mini Whole Gel Eluter

Brian J. Shiell and Wojtek P. Michalski, CSIRO Division of Animal Health,Australian Animal Health Laboratory, Geelong, Victoria, Australia Introduction Amino acid sequence and composition analyses of proteins can be performed on samples derived from polyacrylamide gels. Protein electro-elution, the most c...

PCR reaction components

The quality of the template influences the outcome of the PCR. For instance, large amounts of RNA in a DNA template can chelate Mg2+ and reduce the yield of the PCR. Also, impure templates may contain polymerase inhibitors that decrease the efficiency of the reaction. Note: To get the purest...

S.E.T.S. Swab Extraction Tube System

A New Tool for the Rapid and Efficient Extractionfrom Swabs in Research Samples Swabs are commonly used for sample extraction from avariety of sites, and for the subsequent detection ofinfectious agents (bacteria, fungi, or viruses)...

Efficient Cellular Fractionation for RNA and Protein Isolation

Efficient Cellular Fractionation for RNA Ambion's Protein and RNA Isolation System, PARIS, uses a rapid and simple procedure to isolate both total RNA and native protein from the same experimental sample. Cultured cells and tis...

InstaGene Matrix: Prepare DNA Templates for PCR With No Phenol/Chloroform Extractions and No Deproteinization Steps, Rev C

Prepare DNA from blood, cultured cells, or bacteria: Just incubate sample with InstaGene matrix at 56C for 1530 min, then boil for 8 min. InstaGene matrix absorbs cell lysis products, producing an improved substrate for PCR amplification. Use whol...

Sample Preparation Solutions for Cellular Protein Fractionation Poster, Rev A

T Slyker, B Gillece-Castro, M Nguyen, L Sapp, MG Brubacher, and WB Strong Presented by the Authors at The Association of Biomolecular Resource Facilities (ABRF) Denver, Colorado 2-D gel electrophoresis (2DE) is a widely used, proven method for...

Separation of Peptides by Hydrophilic Interaction Chromatography Using TSKgel Amide-80

Authors: T.J. Higley, Tosoh Bioscience LLC, Tatsunari Yoshida, Tosoh Corporation The TSKgel Amide-80 column provides excellent selectivity for separating peptides in hydrophilic interaction chromatography due to polar carbamoyl functionalities bonded to a silica base material....

Video game learning has no traction in K-12

Someday, the curriculum for physics, history, engineering, or management classes could be delivered through computerized simulations, an idea that some members of the <a href="http://w...

Lights, cameras, action: The state of online video

. YouTube, a video sharing site and Web 2.0 phenomena, has well over 34 million unique visitors spending almost 30 minutes monthly on the s...

Cell-phone satisfaction stays low, says Consumer Reports

Less than half of cell-phone users really like the service they are getting, a Consumer Reports survey shows. The survey asked 50,000 people to recount experiences with static, "dead" zones, dropped calls and more, and ended up finding that cell-phone service is one of the lowest-ranked services nationwide in consumer's minds. And to top it off, almost a third of cell-phone users are ser...

Video game technology could improve physician-patient interactions

The development of the game is the next step in the life of Fred Kron, an assistant clinical professor at the <a href...

Business blogs increase customer interactions

According to Tom Pionek, the company's technology and marketing director, the blog can bring Stone Creek Coffee an ability to communicate...

TDS takes top spot in region in customer satisfaction rankings

TDS results in the represent a combined score of...

Brand Bullies- Entrepreneurs draw legal action over common words

Before the Janesville fixed-base airport operator and entrepreneur applied for a name with the , he checked to see if anyone else had a similar n...

Action now can help avoid the coming shortage of math and science grads

In 1975, the United States was third among all nations in the percentage of 18-to-24-year-olds who earned science and engineering degrees. Today, it ranks 17th, behind Taiwan and South Korea, Ireland and Italy. Unless that slide in scientific competency is reversed, the nations economic welfare and security will be threatened. That was the conclusion of a report issued in May 2004 by the...

API Software best in health-care customer satisfaction, says KLAS report

HARTFORD, Wis. rankings of health information technology vendor performance. In the mid-year rankings API received the highest possible distinction in customer satisfactio...

Fiserv Credit Processing Selects System To Keep Transactions Quick

Brookfield, Wis.-- 's MAINVIEW systems management solutions to help ensure response time of its credit card and credit application processing needs. Fiserv Credit Processing Services provides outsourced credit receivables management s...

eCareSolutions to Launch Patient Interaction Product

A Mequon-based developer of personalized medicine tools for use by self-insured companies plans to launch a product next year that will use the Web to prod patients to lower their health risks. Currently, offers services that allow employers to predict health risk exposure and help insured employees w...