AMAX

Fluorometric Protease Assays in the SpectraMax Gemini Microplate Spectrofluorometer: Example Using Caspase3 (MaxLine Application Note #35)

Evelyn McGown, Ph.D. and Anna Lam, B.S. Molecular Devices Corporation, 8/99 Modern assays for proteolytic activity typically use synthetic chromogenic or flu-orogenic peptide substrates. Enzyme selectivity is obtained by choosing a peptide sequence uniquely recognized by the catalytic site of a particular enzy...

LIVE/DEAD Viability/Cytotoxicity Assay for Animal Cells Using the SpectraMax Gemini XS Fluorescence Microplate Reader (MaxLine Application Note #43)

Anne T. Ferguson, Ph.D. Molecular Devices Corporation This application note describes the use of the SPECTRAmax Viability/Cytoxicity Assay Kit for animal cells. This assay uses two fluorescent dyes, calcein AM (cal...

Measurement of Green Fluorescent Protein in the SpectraMax Gemini XS Spectrofluorometer (MaxLine Application Note #44)

1 GFP (Green Fluorescent Protein) is an extremely stable protein of 238 amino acids produced by the jellyfish that fluoresces in the lower green region of the visible spectrum....

Measurement of Molecular Beacons in the SpectraMax Gemini Spectrofluorometer (MaxLine Application Note #36)

October 1999 Molecular beacons are fluorogenic oligonucleotide probes developed by Kramerand associates to detect specific nucleic acids in homogeneous solutions. They are single-stranded oligonucleotides that exist in a hairpin shape because of thecomplementary arms at the ends which bind together to form a stem....

Using the NanoOrange protein kit in the Gemini XS, Gemini EM and SpectraMax M2 microplate readers (MaxLine Application Note #22)

By Yan Zhang, Molecular Devices Corporation, 1311 Orleans Dr., Sunnyvale, CA 94089. This application note describes how to use the NanoOrange Pro software from Mo...

Using the OligoGreen Oligonucleotide Quantitation Reagent in the Gemini XS, Gemini EM and SpectraMax M2 Microplate Readers (MaxLine Application Note #21)

By Yan Zhang., Molecular Devices Corporation, 1311 Orleans Dr., Sunnyvale, CA 94089. This application note describes how to use the OliGreen Pro software from...

PathCheck Applied to Measurement of Protein Solutions in the SpectraMax Plus Microplate Spectrophotometer (MaxLine Application Note #26)

Protein concentration is commonly estimated by measuring the absorbance at 280 nm and calculating the concentration using the extinction coefficient of the protein. The procedure is feasible because extinction coefficients are typically obtained using standard cuvettes with a fixed 1 cm pathlength. Extinction-based protein assays can also...

Microdetermination of Phosphorus Using the SpectraMax Plus Microplate Spectrophotometer: Choice of Microplate, Cuvette, or Test Tube Assay Formats (MaxLine Application Note #24)

Most spectrophotometric methods for inorganic phosphorus (Pi) analysis are based on the color formed upon the reduction of a phosphomolybdate complex. The classic (and still widely-used) method is that of Fiske and Subbarow , in which the reduction is carried out with sulfite and aminonaphtholsulfonic acid. Over the years, modi...

UV Absorbance Measurements in SpectraMax Microplate Spectrophotometers MaxLine Application Note #32)

Molecular Devices Corporation. 7/99 In 1994, Molecular Devices introduced SPECTRAmax , the first microplate reader equipped with a monochromator, rather than bandpass filters, for wavelength selection. It was also the first microplate reader capable of measuring absorbance below 340 nm. These new features transformed the micropl...

Using the SpectraMax Plus for USP Dissolution Calibration (MaxLine Application Note #27)

Calibration of a dissolution apparatus is necessary to produce accurate results for dissolution testing of pharmaceutical dosage forms and to allow comparison between batches and between laboratories. USP requirements for calibration are given in the Apparatus Suitability Test in USP Each individual appa...

Verifying Multichannel Pipettor Performance with Standard Dispense Solutions in the SpectraMax Plus (MaxLine Application Note #28)

Pipettors often fail to meet the manufacturers stated specifications and pipetting is a major source of laboratory error even when the pipettor is operating properly. Accuracy and precision specifications typically provided by pipettor manufacturers are obtained under ideal conditions. In the laboratory, performan...

measuring cell proliferation with SpectraMax M5, M2 and Gemini microplate readers using the CyQUANT Cell Proliferation Assay

Cathy Olsen, Ph.D., Molecular Devices Corporation, 1311 Orleans Dr., Sunnyvale, CA 94089....

Obtaining a contour map from multifiles or 3D data in DataMax

DataMax is the software used to display data from all SPEX instruments. DataMax can present the measurements in many ways, including a contour map. A contour map displays multifiles or 3-D data, and looks much like a geographical map of heights above sea-level, or a meteorological plot of barometric pressures. X values are...

Oshkosh's TerraMax rolls across finish line

, one of five t...