Glutathione-S-Transferase (GST) Colorimetric Assay Kit from Sigma-Aldrich
Description:Biochem/physiol Actions: Glutathione-S-transferases (GSTs) are a group of enzymes important in the detoxification of many xenobiotics in mammals. The enzymes protect cells against toxicants by conjugating the thiol group of the glutathione to electrophilic xenobiotics, and thereby defend cells against the mutagenic, carcinogenic, and toxic effects of the compounds. GST activity is presen...S. cerevisiae Direct Transformation Kit from Wako Chemicals USA, Inc.
Description:This kit is designed for easy transformation of the budding yeast, Saccharoyces sp.. The protocol allows for successful transformation simply by mixing a plasmid DNA and the kit reagents with cultured yeast cells. No complicated steps, such as centrifugation or cell washing, are required. The kit is particularly well suited for high-throughput transformation of a large number of yeast strains gro...S. cerevisiae Direct Transformation Kit from Wako Chemicals USA, Inc.
Description:This kit is designed for easy transformation of the budding yeast, Saccharoyces sp.. The protocol allows for successful transformation simply by mixing a plasmid DNA and the kit reagents with cultured yeast cells. No complicated steps, such as centrifugation or cell washing, are required. The kit is particularly well suited for high-throughput transformation of a large number of yeast strains gro...GenomONE-Neo EX HVJ-E 4 vial(s) Transfection Reagents from Cosmo Bio Co., Ltd.
Description:HVJ (hemagglutinating virus of Japan, also named Sendai virus) Envelope VECTOR KIT is a tool for transfection of molecules by means of membrane fusion. Most efficient transfection are for siRNA (RNAi), Protein, Oligo, cDNA, Antibodies, Vector, and Biomolecule in vitro and in vivo. GenomONE-Neo EX has a high fusing capability in short period of time that benetif sensitive primary cells or tissues...Ribonucleic acid, transfer from baker's yeast (S. cerevisiae) from Sigma-Aldrich
Description:lication: For use as a carrier in nucleic acid purification and precipitation. Physical Form: buffered aqueous solution...Ribonucleic acid, transfer from baker's yeast (S. cerevisiae) from Sigma-Aldrich
Description:Preparation Note: Prepared by a modification of the procedure of Hancher, C.W., et al., Biotechnol. Bioeng., Type: Type X-SA...Ribonucleic acid, transfer from baker's yeast (S. cerevisiae) from Sigma-Aldrich
Description:Analysis Note: Evaluated for its amino acid acceptor activity using aminoacyl-tRNA synthetase and a mixture of amino acids Ala, Asp, Gly, Ile, Lys, Pro, Thr, Val, Arg, Glu, His, Leu, Phe, Ser, Tyr. Type: Type X...Ribonucleic acid, transfer from baker's yeast (S. cerevisiae) from Sigma-Aldrich
Description:Application: For use as a carrier in nucleic acid purification and precipitation. Physical Form: buffered aqueous solution...Transformance™ competent cell preparation service from Lucigen
Description:Do you need to make your strain of cells competent or increase their transformation efficiency? Take advantage of Lucigen’s unrivalled expertise in producing competent cells. Lucigen’s Transformance Custom Competent Cell Service offers: Quality control p...Glutathione-S-Transferase-pi EIA Kit from Alpco Diagnostics
Description:Glutathion-S-Transferase-pi EIA 25 l...Cell Transformation Colorimetric Assay Kit from CHEMICON
Description:CHEMICON's Cell Transformation Detection Assay is an anchorage-independent growth assay in soft agar, which is considered the most stringent assay for detecting malignant transformation of cells. For this assay, cells (pre-treated with carcinogens or carcinogen inhibitors) are cultured with appropriate controls in soft agar medium for 21-28 days. Following this incubation period, formed colonie...Label IT siRNA Tracker Fluorescein Kit with TransIT-siQUEST Reagent from Mirus Bio Corporation
Description:The Label IT siRNA Tracker Intracellular Localization Kit with TransIT-siQUEST Transfection Reagent provides a straight forward approach to directly label and deliver siRNA, of your design, in an efficient yet non-destructive manner, for in vitro tracking experiments. Subcellular localization and functional inhibition of target gene expression can be monitored following introduction of the labele...Label IT siRNA Tracker CX Rhodamine Kit with TransIT-siQUEST Reagent from Mirus Bio Corporation
Description:The Label IT siRNA Tracker Intracellular Localization Kit with TransIT-siQUEST Transfection Reagent provides a straight forward approach to directly label and deliver siRNA, of your design, in an efficient yet non-destructive manner, for in vitro tracking experiments. Subcellular localization and functional inhibition of target gene expression can be monitored following introduction of the labele...Label IT siRNA Tracker TM Rhodamine Kit with TransIT-siQUEST Reagent from Mirus Bio Corporation
Description:The Label IT siRNA Tracker Intracellular Localization Kit with TransIT-siQUEST Transfection Reagent provides a straight forward approach to directly label and deliver siRNA, of your design, in an efficient yet non-destructive manner, for in vitro tracking experiments. Subcellular localization and functional inhibition of target gene expression can be monitored following introduction of the labele...Label IT siRNA Tracker Biotin Kit with TransIT-siQUEST Reagent from Mirus Bio Corporation
Description:The Label IT siRNA Tracker Intracellular Localization Kit with TransIT-siQUEST Transfection Reagent provides a straight forward approach to directly label and deliver siRNA, of your design, in an efficient yet non-destructive manner, for in vitro tracking experiments. Subcellular localization and functional inhibition of target gene expression can be monitored following introduction of the labele...Label IT siRNA Tracker Cy3 Kit with TransIT-siQUEST Reagent from Mirus Bio Corporation
Description:The Label IT siRNA Tracker Intracellular Localization Kit with TransIT-siQUEST Transfection Reagent provides a straight forward approach to directly label and deliver siRNA, of your design, in an efficient yet non-destructive manner, for in vitro tracking experiments. Subcellular localization and functional inhibition of target gene expression can be monitored following introduction of the labele...jetSI-ENDO-FluoR from Polyplus Transfection
Description:jetSI-ENDO-FluoR is a tetramethylrhodamine-conjugated jetSI-ENDO (excitation at 555 nm; emission at 580 nm), the powerful siRNA transfection reagent. These reagents are used to determine the transfection efficiency or investigate the intracellular delivery and trafficking of siRNAs. More than 95 % of the cell population is transfected....TransIT-siQUEST Transfection Reagent from Mirus Bio Corporation
Description:Cellular uptake of long double stranded RNA (dsRNA) has been shown to induce RNA interference in a diverse group of organisms as well as insect cells in culture. RNA interference leads to the inhibition of protein expression by utilizing sequence-specific, dsRNA-mediated destruction of the target messenger RNA (mRNA). Attempts to induce RNA interference using long dsRNA in mammalian cell lines ha...jetSI-ENDO from Polyplus Transfection
Description:jetSI-ENDO is an easy-to-use siRNA transfection reagent that has been shown to knock out endogenous genes in a variety of cell lines. jetSI-ENDO is serum compatible and comes with an optimized, straightforward protocol. High levels of gene silencing (up to 80%) are obtained following transfection of 20 to 100 nM of siRNA in a stably expressing reporter gene cell line, A549Luc....TransIT-siQUEST Transfection Reagent from Mirus Bio Corporation
Description:Cellular uptake of long double stranded RNA (dsRNA) has been shown to induce RNA interference in a diverse group of organisms as well as insect cells in culture. RNA interference leads to the inhibition of protein expression by utilizing sequence-specific, dsRNA-mediated destruction of the target messenger RNA (mRNA). Attempts to induce RNA interference using long dsRNA in mammalian cell lines ha...TransIT-TKO siRNA Transfection Reagent from Mirus Bio Corporation
Description:One step, serum compatible reagent supplied with an optimized protocol....jetSI-ENDO-FluoF from Polyplus Transfection
Description:jetSI-ENDO-FluoF is a fluorescein-conjugated jetSI-ENDO (excitation at 490 nm; emission at 520 nm), the powerful siRNA transfection reagent. These reagents are used to determine the transfection efficiency or investigate the intracellular delivery and trafficking of siRNAs. More than 95 % of the cell population is transfected....INTERFERin from Polyplus Transfection
Description:INTERFERin is a new-generation siRNA transfection reagent that achieves over 90 % silencing efficiency at 1 nM siRNA in a wide variety of adherent and primary cells with excellent cell viability. Using low siRNA concentrations avoids unwanted toxic and off-target (non-specific) effects observed when transfecting siRNA at higher concentrations, thereby allowing accurate and reliable silencing resu...jetSI conc. 10 mM from Polyplus Transfection
Description:This special concentrated jetSI 10 nM is available for delivery into mouse brain as described in: Z. Hassani, G.-F. Lemkine, P. Erbacher, G. Alfama, C. Giovannangeli, J.-P. Behr, and B.-A. Demeneix. Lipid-mediated siRNA delivery down regulates exogenous gene expression in the mouse brain at picomolar levels. J. Gene Med., 2005, 7, 198-207....Frozen-EZ Yeast Transformation II Kit from Zymo Research
Description:The transformation of Saccharomyces cerevisiae, as well as other yeasts, has become a routine procedure in many molecular biology laboratories. The ability to transform whole cells at high efficiencies with plasmid and linear DNAs using lithium based buffers and polyethylene glycol has made routine plasmid transformations and library screening easy and reliable, especially when compared to techni...siPORT NeoFX Transfection Agent 1 ml from Ambion
Description:Gene silencing experiments often call for critical, but time-consuming optimization experiments. siPORT NeoFX Transfection Agent refines siRNA transfection protocols resulting in less optimization. siPORT NeoFXs lipid-based formulation can be used to efficiently transfect adherent cells as they are subcultured without increased cytotoxicity. From start to finish, successful gene silencing experi...siPORT NeoFX Transfection Agent from Ambion
Description:Gene silencing experiments often call for critical, but time-consuming optimization experiments. siPORT NeoFX Transfection Agent refines siRNA transfection protocols resulting in less optimization. siPORT NeoFXs lipid-based formulation can be used to efficiently transfect adherent cells as they are subcultured without increased cytotoxicity. From start to finish, successful gene silencing experi...Quixell Cell Transfer System from Stoelting Co.
Description:Quixell, manufactured by Stoelting, selects single cells, or clusters, with rapid, smooth, precise control. In no time at all, a single cell can be targeted, then click-click on the keypad...the cell is collected into the pipette, and automatically transferred to the individual wells of a microtiter plate. Quixells unique aspiration and expulsion system allows transfer of 1-5 cells at a time, int...Terminal deoxynucleotidyl transferase (TdT) Immunofluorescence Assay Kit from AbD Serotec
Description:Terminal deoxynucleotidyl transferase (TdT) is an intracellular marker normally present at high levels in cortical thymocytes and minor subpopulation of bone marrow prelymphocytes. Elevated levels of the enzyme and increased numbers of TdT cells are found to be associated with some lymphoblastic leukaemias and lymphomas. Presence of TdT thus provides a useful adjunct in the differentiation of leu...Guanylyltransferase (5 U/l) from Ambion
Description:Request Info...EZ-Tn5 Transposase from EPICENTRE Biotechnologies
Description:EZ-Tn5 Transposase is designed for use with our pMOD series of vectors as well as any EZ-Tn5 transposon system....M-MLV Reverse Transcriptase from USB Corp.
Description:Source: E. coli strain containing an overproducing clone of M-MLV Reverse Transcriptase. 10 kb, may be synthesized. M-MLV Reverse Transcriptase has a much lower RNase H activity than AMV Reverse Transcriptase resulting in high yields of full l...AMV Reverse Transcriptase from Promega
Description:Avian Myeloblastosis Virus Reverse Transcriptase (AMV RT) catalyzes the polymerization of DNA using template DNA, RNA or RNA:DNA hybrids (1). It requires a primer (DNA primers are more efficient than RNA primers) as well as Mg2+ or Mn2+. The enzyme possesses an intrinsic RNase H activity. Both nonionic detergents and sulfhydryl compounds stabilize the enzyme activity in vitro....Reverse Transcriptase AMV; RNA dependent DNA polymerase from Roche Applied Science
Description:Request Info...