26S Proteasome Fraction Extract from GeneTex
Description:Proteolytic degradation is critical to the maintenance of appropriate levels of short-lived and regulatory proteins as important and diverse as those involved in cellular metabolism, heat shock and stress response, antigen presentation, modulation of cell surface receptors and ion channels, cell cycle regulation, transcription, and signalling factors. The ubiquitin-proteasome pathway deconstructs...Ubiquitin/Proteasome Fraction II Extract from GeneTex
Description:Proteolytic degradation is critical to the maintenance of appropriate levels of short-lived and regulatory proteins as important and diverse as those involved in cellular metabolism, heat shock and stress response, antigen presentation, modulation of cell surface receptors and ion channels, cell cycle regulation, transcription, and signalling factors. The ubiquitin-proteasome pathway deconstructs...20S Proteasome Fluorometric (AMC) Assay Kit from CHEMICON
Description:The ubiquitin-proteasome pathway is the major proteolytic system in the cytosol of eukaryotic cells, where it catalyzes the selective degradation of short-lived regulatory proteins and the rapid elimination of proteins with abnormal conformation (Hershko & Ciechanover 1998; Hochstrasser 1996). The critical protease in this pathway is the 26S proteasome, an ATP-dependent proteolytic complex, which...26S Proteasome Fraction Extract from GeneTex
Description:Proteolytic degradation is critical to the maintenance of appropriate levels of short-lived and regulatory proteins as important and diverse as those involved in cellular metabolism, heat shock and stress response, antigen presentation, modulation of cell surface receptors and ion channels, cell cycle regulation, transcription, and signalling factors. The ubiquitin-proteasome pathway deconstructs...Factor Xa Protease from QIAGEN
Description:Efficient 6xHis tag cleavage (greater than 90%)...AcTEV Protease from Invitrogen
Description:AcTEV Protease is an enhanced form of Tobacco Etch Virus (TEV) protease that is highly site-specific, highly active, and significantly more stable than native TEV protease, resulting in long-term activity. AcTEV Protease specifically recognizes a seven amino acid (Glu-Asn-Leu-Tyr-Phe-Gln-Gly, cleaving between Gln and Gly), making it useful for removing affinity tags from fusion proteins (1,2). Fo...PROTEAN Plus Overlay Agarose, 125 ml from Bio-Rad
Description:PROTEAN Plus overlay agarose is used for second-dimension SDS-PAGE sample setup to secure IPG strips in place in PROTEAN Plus cells. Bromophenol Blue tracking dye is incorporated to allow monitoring of electrophoresis runs. The solution is composed of 0.75% agarose in 1x Tris, glycine, SDS, and 0.003% Bromophenol Blue and is supplied as 125 ml....SDSaway from Protea Biosciences, Inc.
Description:The SDSaway reagent is a valuable tool for removing SDS detergent from protein samples. The presence of SDS in biological samples imparts a negative charge to the tightly bound SDS-protein complex, inhibits enzymatic activity during digestion, and suppresses ion signals and presents as a contaminant in mass spectrometry analyses. We recommend that SDSaway be used with Proteas PILFer (In-line pr...PROTEAN II xi Cell, 20 cm, without spacers and combs from Bio-Rad
Description:The PROTEAN II xi cell, 20 cm, is used for vertical slab gel electrophoresis of nucleic acids and proteins in agarose gels. The cell is supplied without spacers and combs. The unit includes a central cooling core with gaskets, lower buffer chamber, lid with cables, 2 sets of glass plates, 4 sandwich clamps, upper buffer dam, casting stand with gaskets, leveling bubble, and instructions....PROTEAN II xi Cell, 16 cm, without spacers and combs from Bio-Rad
Description:The PROTEAN II xi cell, 16 cm, is used for vertical slab gel electrophoresis of nucleic acids and proteins in agarose gels. The cell is supplied without spacers and combs. The unit includes a central cooling core with gaskets, lower buffer chamber, lid with cables, 2 sets of glass plates, 4 sandwich clamps, upper buffer dam, casting stand with gaskets, leveling bubble, and instructions....PROTEAN II XL Cell, wide format from Bio-Rad
Description:Includes PROTEAN II xi Basic unit and IPG conversion kit 2.0 mm 165-3190...PROTEAN II xi Multi-Cell from Bio-Rad
Description:The PROTEAN II xi multi-cell is for 2-D separations. The cell has a six-gel capacity with efficient cooling capabilities. The PROTEAN II xi multi-cell can be customized for several parameters: gel length, comb, type of glass plates, and cooling system. The PROTEAN II xi multi-cell can also be converted to the PROTEAN II XL multi-cell for a wider format that accommodates 17 and 18 cm IPG strips. P...PROTEAN II XL Cell, wide format, 2.0 mm from Bio-Rad
Description:The PROTEAN II XL cell, wide format, is used for vertical electrophoresis applications. The cell accommodates two 18.5 x 20 cm gels and requires 1.2 L of running buffer. It includes the PROTEAN II xi basic unit and the 2.0 mm IPG conversion kit, which contains accessories for use with 2.0 mm thick gels and includes 2 sets of IPG clamps, 2 sets of 20 x 20 cm glass plates, 4 IPG spacers, 2 IPG comb...PROTEAN II xi Multi-Cell 2-D Conversion Kit from Bio-Rad
Description:The PROTEAN II xi multi-cell 2-D conversion kit converts the PROTEAN II xi multi-cell to the PROTEAN II XL multi-cell for a wider format cell that accepts 17 to 18 cm IPG strips....PROTEAN II xi Cell 2-D Conversion Kit. from Bio-Rad
Description:Kit includes 2 tube gel adaptors, 24 glass tubes (1.5 mm ID, 180 mm length), gaskets, grommets, stoppers. The PROTEAN II xi cell and PROTEAN II XL wide-format cell offer the versatility to perform a variety of separations,* including SDS-PAGE, first- and second-dimension 2-D electrophoresis, native, preparative, and gradient PAGE as well as high-resolution agarose electrophoresis of nucleic acid...Mini-PROTEAN II Multiscreen Apparatus from Bio-Rad
Description:The Mini-PROTEAN II multiscreen apparatus is used to screen blots of mini format (8 x 7 cm) gels. The apparatus can screen up to 40 different antibodies or sera without having to cut the blot into strips. The apparatus includes a base plate, 2 separate, detachable sample templates that accommodate one or two 8 x 7 cm membranes, and gaskets and clamps that secure the blots to form 40 channels (eac...Mini-PROTEAN II Multiscreen Apparatus from Bio-Rad
Description:...PROTEAN II xi 2-D Cell from Bio-Rad
Description:The PROTEAN II xi 2-D system runs both gel dimensions in the same cell. Up to 16 first-dimension IEF tube gels can be separated in a single run using the tube gel adaptors, which snap onto the cooling core in much the same manner as the slab gel assembly. While the tube gels are running, the second-dimension slab gels may be cast in the casting stand. The 20 cm long slab gel option provides extra...PROTEAN II Slab Gel Casting Stand, with gaskets from Bio-Rad
Description:The PROTEAN II slab gel casting stand is a component of the PROTEAN II XL and xi casting apparatus. It is supplied with gaskets....Protease Inhibitor Cocktail Set I from Calbiochem
Description:...Mini-PROTEAN 3 Dodeca Cell With Multi-Casting Chamber from Bio-Rad
Description:The Mini-PROTEAN 3 Dodeca cell with multi-casting chamber is used for handcasting and vertical electrophoresis of up to 12 mini format handcast (8.3 x 7.3 cm) or precast (8.6 x 6.8 cm) gels. The cell requires 3.4-4.4 L of buffer and includes an electrophoresis tank with built-in cooling coil that attaches to an external refrigerated circulator (not included), lid with power cables, 6 electrophore...Accumix from Protea Biosciences, Inc.
Description:Our Accumix standard is a new tuning and calibration standard for positive ion mode electrospray ionization mass spectrometry. Accumix is a lyophilized 7-peptide standard that provides broad mass range coverage for both tuning and calibration of ion trap (i.e. Finnigan LCQ- and LTQ-MS) and Fourier transform mass spectrometers. Direct infusion of the Accumix peptide standard produces 9 major par...MS Grade Protein Standards from Protea Biosciences, Inc.
Description:Protea is pleased to introduce its new line of Protein Standards, a growing family of lyophilized standard proteins for use in mass spectrometry, molecular biology, and life science laboratories. Proteas Protein Standards are high quality purified proteins for use in the calibration of protein analyses by electrospray ionization mass spectrometry (ESI-MS). For quality control and standardizatio...