Phosphate in Biological Products
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Phosphate in Biological Products
Description: Lipid Phosphate Phosphohydrolase 2...
Company:Exalpha Biologicals, Inc.
Company:Invitrogen
Company:Sigma-Aldrich
Company:Sigma-Aldrich
Company:Sigma-Aldrich
Company:ScyTek Laboratories
Company:Caldon Biotech, Inc.
Company:BioAssay Systems
Company:BioAssay Systems
Company:CHEMICON
Company:CHEMICON
Company:Invitrogen
Company:R&D Systems
Company:Promega
Company:Sigma-Aldrich
Company:Bio-Rad
Company:Sigma-Aldrich
Company:Pierce Biotechnology, Inc.
Company:Pierce Biotechnology, Inc.
Company:Pierce Biotechnology, Inc.
Company:Sigma-Aldrich
Company:Sigma-Aldrich
Company:Sigma-Aldrich
Company:Calbiochem
Company:GeneTex
Description:... T4 DNA Ligase catalyzes the formation of phosphodiester bonds in the presence of ATP between double-stranded DNAs with 3' hydroxyl and 5' phosphate termini. The unique T4 DNA Ligase buffer optimizes ligation which can be performed in 5 minutes (1). Single-stranded nucleic acids are not substrates ...
Company:Invitrogen
Company:Sigma-Aldrich
Company:AppliChem GmbH
Company:Sigma-Aldrich
Company:Sigma-Aldrich
Company:Sigma-Aldrich
Company:Sigma-Aldrich
Company:Thermo Scientific
Company:USB Corp.
Company:USB Corp.
Company:USB Corp.
Description:...ntrasts with E. coli DNA Ligase which requires NAD. Unit Definition: One Weiss unit is the amount of enzyme required to convert 1 nmol of radiolabeled phosphate from pyrophosphate into absorbable material in 20 min at 37C under standard assay conditions. One Weiss unit equals about 67 cohesive end ligation uni...
Company:USB Corp.
Company:EPICENTRE Biotechnologies
Company:EPICENTRE Biotechnologies
Company:EPICENTRE Biotechnologies
Company:Exalpha Biologicals, Inc.
Calcium Phosphate Transfection Kit from Invitrogen
Description: Invitrogen's Calcium Phosphate Transfection Kit provides high-quality reagents to enable the introduction of DNA into tissue culture cells via calcium phosphate co-precipitation....Company:Invitrogen
Rabbit Anti-Lipid Phosphate Phosphatase 2 (LPP2) Antibody, Unconjugated from Sigma-Aldrich
Description: Anti-Lipid Phosphate Phosphatase 2 (LPP2) fractionated antiserum buffered aqueous solution Solution in phosphate buffered saline containing 0.08% sodium azide. unique peptide derived from the human LPP2 protein. The amount of the reagent is sufficient for 10 blots....Company:Sigma-Aldrich
Dulbecco's Phosphate Buffered Saline (DPBS) from Sigma-Aldrich
Description: ...Company:Sigma-Aldrich
Dulbecco's Phosphate Buffered Saline (DPBS) from Sigma-Aldrich
Description: With 36 mg/L sodium pyruvate, 50 mg/L streptomycin sulfate, 100 mg/L kanamycin monosulfate, 1000 mg/L glucose, calcium chloride, and magnesium chloride. The use of a balanced salt solution (BSS) in tissue culture is generally attributed to early workers in the field. In 1885, Sydney Ringer develope...Company:Sigma-Aldrich
Coating Stabilizer from ScyTek Laboratories
Description:...of the dried immunoassay components. Product is filtered at 0.2 microns. Contents: Aqueous, protein-containing stabilizer and blocking solution in phosphate buffer for dried protein components in immunoassays. Preservative: 0.02% Bromonitrodioxane, 0.02% 2-Methyl-4-isothiazolin-3-one. pH 7.2 - 7.7...Company:ScyTek Laboratories
Stat-Pack Sputolysin Reagent from Caldon Biotech, Inc.
Description:... Sterile concentration of Dithiothreitol (DTT) solution in phosphate buffer (pH 7.0). Dilute contents in one vial (10- fold) to 100 ml. For use in liquefying sputum samples....Company:Caldon Biotech, Inc.
Phosphate Colorimetric Assay Kit, PiBlue™ from BioAssay Systems
Description:...assays for phosphatase, lipase, nucleoside triphosphatases (ATPase, GTPase etc). HTS and follow up studies for phosphatase inhibitors. Quantitation of phosphate content in protein, lipids, and DNA....Company:BioAssay Systems
Phosphate Colorimetric Assay Kit from BioAssay Systems
Description:...assays for phosphatase, lipase, nucleoside triphosphatases (ATPase, GTPase etc). HTS and follow up studies for phosphatase inhibitors. Quantitation of phosphate content in protein, lipids, and DNA....Company:BioAssay Systems
LIGHT DIAGNOSTICS™ CMV pp65 Dextran Leukocyte Separation Kit, included in kit #3247 from CHEMICON
Description:...220 mL of PBS, Nonidet P-40, sucrose, protein stabilizer and 0.5% sodium azide. Phosphate-Buffered Saline (PBS) - (Catalog No. 5087) Three packets of phosphate buffered saline salts. Wash Supplement (100X) - (Catalog No. 5117) One bottle containing 30 mL of protein solution and 0.1% sodium azide. ...Company:CHEMICON
LIGHT DIAGNOSTICS™ CMV pp65 NH4Cl Leukocyte Separation Kit, alternate to #3246 from CHEMICON
Description:...220 mL of PBS, Nonidet P-40, sucrose, protein stabilizer and 0.5% sodium azide. Phosphate-Buffered Saline (PBS) - (Catalog No. 5087) Three packets of phosphate buffered saline salts. Wash Supplement (100X) - (Catalog No. 5117) One bottle containing 30 mL of protein solution and 0.1% sodium azide. ...Company:CHEMICON
T4 Polynucleotide Kinase Exchange Reaction Buffer from Invitrogen
Description:... For use with T4 Polynucleotide Kinase for labeling of 5' ends (5' phosphate ends) of nucleic acids. In the exchange reaction T4 Polynucleotide Kinase transfers the 5' phosphate group from DNA to ADP and subsequently transfers the Gamma-phosphate of ATP...Company:Invitrogen
Phosphate Colorimetric Assay Kit from R&D Systems
Description:... This Malachite Green phosphate Detection Kit...Company:R&D Systems
Calf Alkaline Phosphatase, Calf Intestinal, Unconjugated from Promega
Description:...o- and deoxyribonucleoside triphosphates. This enzyme is used to prevent recircularization and religation of linearized cloning vector DNA by removing phosphate groups from both 5-termini and may also be used for the dephosphorylation of 5 phosphorylated ends of DNA or RNA for subsequent labeling with [32P]ATP...Company:Promega
Rabbit Anti-Lck, phospho (Tyr192) Antibody, Unconjugated from Sigma-Aldrich
Description:...d to remove any reactivity towards a non-phosphorylated Lck. affinity isolated antibody buffered aqueous glycerol solution A solution in Dulbecco's phosphate buffered saline (without Mg 2+ and Ca 2+ ), pH 7.3 (+/- 0.1), 50% glycerol, with 1.0 mg/mL BSA (IgG, protease free) and 0.05% sodium azide A syntheti...Company:Sigma-Aldrich
Western Blot Amplification Module from Bio-Rad
Description:... Kit contains: Bio-Rad Amplification Reagent, 2x Amplification Diluent, Streptavidin-HRP, Blocking Reagent, and phosphate Buffered Saline....Company:Bio-Rad
Rabbit Anti-Hck, phospho (Tyr209 / Ser211) Antibody, Unconjugated from Sigma-Aldrich
Description:... Anti-phospho-Hck [pTyr 209/ pSer 211 ] affinity isolated antibody aqueous solution a solution in Dulbecco's phosphate buffered saline (without Mg 2+ and Ca 2+ ), pH 7.3, with 1.0 mg/ml BSA (IgG and protease free) and 0.05% sodium azide. a synthetic phosphorylated pep...Company:Sigma-Aldrich
DISCOVERLIGHT BIOTIN ARRA from Pierce Biotechnology, Inc.
Description:.... Includes: DiscoverLight Nitrocellulose Membrane: 4 each Blocker BSA in PBS (10X): 50 ml Streptavidin HRP-conjugated: 0.1 mg BupH phosphate Buffered Saline: 8 packs 10% Tween-20: 2 x 10 ml ampules SuperSignal West Pico Luminol Enhancer: 2 x 25 ml SuperSignal West Pico Stable Peroxid...Company:Pierce Biotechnology, Inc.
DISCOVERLIGHT 6XHIS ARRAY from Pierce Biotechnology, Inc.
Description:...ple elements. Includes: DiscoverLight Nitrocellulose Membrane: 4 each Blocker BSA in PBS (10X): 50 ml INDIA HisProbe HRP: 0.2 mg BupH phosphate Buffered Saline: 8 packs 10% Tween-20: 2 x 10 ml ampules SuperSignal West Pico Luminol Enhancer: 2 x 25 ml SuperSignal West Pico Stable Peroxide...Company:Pierce Biotechnology, Inc.
DISCOVERLIGHT MOUSE ARRAY from Pierce Biotechnology, Inc.
Description:...overLight Nitrocellulose Membrane: 4 each Blocker BSA in PBS (10X): 50 ml Stabilized Goat Anti-Mouse Antibody, HRP-conjugated: 10 g BupH phosphate Buffered Saline: 8 packs 10% Tween-20: 2 x 10 ml ampules SuperSignal West Pico Luminol Enhancer: 2 x 25 ml SuperSignal West Pico Stable Pero...Company:Pierce Biotechnology, Inc.
Mouse Anti-G alpha o Monoclonal Antibody, Unconjugated, Clone 1C.2 from Sigma-Aldrich
Description:... Monoclonal Anti-Gα o purified immunoglobulin buffered aqueous solution Solution in phosphate buffered saline pH 7.4, containing 0.08% sodium azide. partially purified bovine brain Gα O protein. ...Company:Sigma-Aldrich
Rabbit Anti-Matrix Metalloproteinase-2, Hinge Region Antibody, Unconjugated from Sigma-Aldrich
Description:...native protein. It reacts with free MMP-2 or MMP-2 bound to TIMP-2. affinity isolated antibody buffered aqueous glycerol solution Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 50% glycerol and 15 mM sodium azide. synthetic peptide corresponding to the hinge region of human matrix metallopr...Company:Sigma-Aldrich
Mouse Anti-Human Angiogenin Monoclonal Antibody, Unconjugated, Clone 14017.7 from Sigma-Aldrich
Description:... Immunogen: purified recombinant human angiogenin expressed in E. coli . Physical form: Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline. Titertest method: ELISA (c). WB. Titertest values: capture ELISA 1-2 μg/mL immunoblotting 1-2 μg/mL...Company:Sigma-Aldrich
Human Cathepsin D from Calbiochem
Description:... Lyophilized. Lyophilized from 2 mM sodium phosphate buffer, pH 6.5. Cathepsin D from human liver. Cathepsin D is a major lysosomal aspartyl protease in mammalian cells produced as a 52 kDa proenzyme. Ov...Company:Calbiochem
10X Automation Buffer from GeneTex
Description:...H of working solution will be 7.5+/- 0.1 Alternatively: -- Substitute 10mM phosphate for Tris Base. -- Substitute 10mM phosphate for Tris Base....Company:GeneTex
Description:... T4 DNA Ligase catalyzes the formation of phosphodiester bonds in the presence of ATP between double-stranded DNAs with 3' hydroxyl and 5' phosphate termini. The unique T4 DNA Ligase buffer optimizes ligation which can be performed in 5 minutes (1). Single-stranded nucleic acids are not substrates ...
Company:Invitrogen
Goat Anti-SDF-1 beta / PBSF Polyclonal Antibody, Unconjugated from Sigma-Aldrich
Description:... Anti-SDF-1β/PBSF affinity isolated antibody lyophilized powder Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline recombinant human SDF-1β/PBSF expressed in E. coli . ...Company:Sigma-Aldrich
Dithio-bis(2-nitrobenzoic acid) BioChemica from AppliChem GmbH
Description:...xture, producing the yellow 4-nitrothiolate. The release of this yellow anion is followed at 412 nm. The assay may be performed at 30C in 0.1 M sodium phosphate buffer with 10 mM DTNB and 12.5 mM acetylthiocholine iodide (1). Stability: Solutions of DTNB are not very stable. You may keep them refrigerated for ...Company:AppliChem GmbH
Rabbit Anti-Human D4 Dopamine Receptor Antibody, Unconjugated from Sigma-Aldrich
Description:...p of human D 4 dopamine receptor, conjugated to KLH. The immunizing peptide has 79% homology with the rat and mouse gene. Physical form: Solution in phosphate buffered saline, pH 7.7, containing 0.01% sodium azide. Titertest method: IHC (p). Titertest values: immunohistochemistry (formalin-fixed, paraffin-...Company:Sigma-Aldrich
Wheat Germ Phosphatase, Acid, Unconjugated from Sigma-Aldrich
Description:..., T.P., J. Biol. Chem., 174, 11 (1948). Quality Clarifier: Contains lipase Unit Definition: One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 4.8 at 37C....Company:Sigma-Aldrich
Shrimp Phosphatase, Alkaline, Unconjugated from Sigma-Aldrich
Description:... containing 25 mM Tris-HCl, pH 7.6, 1 mM MgCl 2 and 0.1 mM ZnCl 2 Preparation Unit Definition: One unit will hydrolyze 1 μmole of 4-nitrophenyl phosphate per minute at pH 9.8 at 37 C. • Concentration: ≥ 1,000 DEA units/mL Physical Form: buffered aqueous glycerol solution...Company:Sigma-Aldrich
Bovine Phosphatase, Alkaline, Unconjugated from Sigma-Aldrich
Description:...sical form: Lyophilized powder containing Tris-citrate buffer salts Preparation Unit Definition: One unit will hydrolyze 1 μmole of 4-nitrophenyl phosphate per minute at pH 9.8 at 37 C. Previously sold in "glycine" units. • Chem comp: Protein ~ 35% (biuret) Physical Form: lyophilized powder...Company:Sigma-Aldrich
Antisense oligonucleotides from Thermo Scientific
Description:... Phosphothioate (PTO) oligonucleotides: PTOs are internucleotide modifications, where one oxygen atom in the phosphate backbone is replaced by a sulphur atom. This modification stabilises the oligonucleotide against nuclease degradation. PTOs are available as Full-PT...Company:Thermo Scientific
Human Topoisomerase II, Alpha from USB Corp.
Description:...: Greater than 98% pure as determined by silver-stained SDS-PAGE. Free of contaminating exonucleases and endonucleases. Storage Buffer: 15mM sodium phosphate (pH 7.1), 700mM NaCl, 0.1mM EDTA, 0.5mM DTT, 50% glycerol. Shipping and Storage: Shipped on dry ice. Store at -20C for frequent use. Store at -80C for...Company:USB Corp.
Calf Topoisomerase I from USB Corp.
Description:... and can be used in solutions containing EDTA. Purity: Tested for contaminating endonucleases and exonucleases. Storage Buffer: 20mM potassium phosphate (pH 7.2), 50mM KCl, 0.05mM EDTA, 5mM 2-mercaptoethanol, 0.02% BSA, 50% glycerol. Shipping and Storage: Shipped on dry ice. Store at -20C. Unit De...Company:USB Corp.
Human Topoisomerase II, Alpha from USB Corp.
Description:...: Greater than 98% pure as determined by silver-stained SDS-PAGE. Free of contaminating exonucleases and endonucleases. Storage Buffer: 15mM sodium phosphate (pH 7.1), 700mM NaCl, 0.1mM EDTA, 0.5mM DTT, 50% glycerol. Shipping and Storage: Shipped on dry ice. Store at -20C for frequent use. Store at -80C for...Company:USB Corp.
Description:...ntrasts with E. coli DNA Ligase which requires NAD. Unit Definition: One Weiss unit is the amount of enzyme required to convert 1 nmol of radiolabeled phosphate from pyrophosphate into absorbable material in 20 min at 37C under standard assay conditions. One Weiss unit equals about 67 cohesive end ligation uni...
Company:USB Corp.
GELase LMT Agarose Gel-Digesting Preparation from EPICENTRE Biotechnologies
Description:...ng point (LMP) agarose gels following electrophoresis in TAE, TBE, MOPS, or phosphate buffers. The gel may be digested directly in the TAE, TBE, MOPS, and phosphate electrophoresis buffers or GELase™ Buffer may be added to or exchanged...Company:EPICENTRE Biotechnologies
GELase LMT Agarose Gel-Digesting Preparation from EPICENTRE Biotechnologies
Description:...ng point (LMP) agarose gels following electrophoresis in TAE, TBE, MOPS, or phosphate buffers. The gel may be digested directly in the TAE, TBE, MOPS, and phosphate electrophoresis buffers or GELase™ Buffer may be added to or exchanged...Company:EPICENTRE Biotechnologies
GELase LMT Agarose Gel-Digesting Preparation from EPICENTRE Biotechnologies
Description:...ng point (LMP) agarose gels following electrophoresis in TAE, TBE, MOPS, or phosphate buffers. The gel may be digested directly in the TAE, TBE, MOPS, and phosphate electrophoresis buffers or GELase™ Buffer may be added to or exchanged...Company:EPICENTRE Biotechnologies
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