NovaBlue Glycerol Stock from Novagen
Description:NovaBlue is a K-12 strain ideally suited as an initial cloning host due to its high transformation efficiency, blue/white screening capability (with appropriate plasmids) and recA endA mutations, which result in high yields of excellent quality plasmid DNA. The DE3 lysogen of NovaBlue is potentially useful as a stringent host due to the presence of the lacIq repressor encoded by the F episome....BBS/Glycerol/Casein Block and Diluent - 10X Concentrate from BioFX Laboratories, Inc.
Description:BBS/Glycerol/Casein Block and Diluent - 10X Concentrate, 1 L...Gel Loading Dye (Bromophenol Blue, Xylene Cyanol/Glycerol) from Mo Bio Laboratories, Inc.
Description:Makes gels easy to load and track during electrophoresis. 10X concentrate of Bromophenol Blue, Xylene Cyanol, glycerol solution in TE...Gel Loading Dye (Bromophenol Blue/Glycerol) from Mo Bio Laboratories, Inc.
Description:Makes gels easy to load and track during electrophoresis. 10X concentrate of Bromophenol Blue, glycerol solution....Bacterial Strain ES1301 mutS, Glycerol Stock (noncompetent) from Promega
Description:BMH 71-18 mutS and ES1301 mutS are mismatch repair minus strains of E. coli. Use of these strains prevents repair of the newly synthesized unmethylated strand (1), leading to high mutation efficiencies and making them helpful in such systems as the GeneEditor and Altered Sites II Mutagenesis Systems. Both ES1301 and BMH 71-18 mutS are recA+, therefore inserts containing highly repetitive sequence...Bacterial Strain BMH 71-18 mutS, Glycerol Stock (noncompetent) from Promega
Description:BMH 71-18 mutS and ES1301 mutS are mismatch repair minus strains of E. coli. Use of these strains prevents repair of the newly synthesized unmethylated strand (1), leading to high mutation efficiencies and making them helpful in such systems as the GeneEditor and Altered Sites II Mutagenesis Systems. Both ES1301 and BMH 71-18 mutS are recA+, therefore inserts containing highly repetitive sequence...Origami(DE3)pLysS Glycerol Stock from Novagen
Description:Origami host strains are K-12 derivatives that have mutations in both the thioredoxin reductase (trxB) and glutathione reductase (gor) genes, which greatly enhances disulfide bond formation in the cytoplasm. Studies have shown that expression in Origami(DE3) yielded 10-fold more active protein than in another host even though overall expression levels were similar (1). Origami hosts are compatibl...Bacterial Strain NM522, Glycerol Stock from Promega
Description:NM522 contains an F episome, which is required for production of ssDNA and for blue/white color selection. To maintain the F, NM522 should be grown on minimal (M-9) medium....Origami(DE3) Glycerol Stock from Novagen
Description:Origami host strains are K-12 derivatives that have mutations in both the thioredoxin reductase (trxB) and glutathione reductase (gor) genes, which greatly enhances disulfide bond formation in the cytoplasm. Studies have shown that expression in Origami(DE3) yielded 10-fold more active protein than in another host even though overall expression levels were similar (1). Origami hosts are compatibl...Gel Loading Dye (Bromophenol Blue/Glycerol) from Mo Bio Laboratories, Inc.
Description:Makes gels easy to load and track during electrophoresis. 10X concentrate of Bromophenol Blue, Xylene Cyanol, glycerol solution in TE...