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Fusion in Biological Products

pET GST Fusion System 41 from Novagen

Description: Schistosomal glutathione-S-transferase (GST) is commonly used as a fusion partner when expressing proteins in E. coli (1). The GSTTag sequence has been reported to enhance the production and in some cases the solubility of its fusion partners. When expressed in a soluble, properly folded form, GS...
Company:Novagen

pET GST Fusion System 41 plus Competent Cells from Novagen

Description: Schistosomal glutathione-S-transferase (GST) is commonly used as a fusion partner when expressing proteins in E. coli (1). The GSTTag sequence has been reported to enhance the production and in some cases the solubility of its fusion partners. When expressed in a soluble, properly folded form, GS...
Company:Novagen

pET GST Fusion System 42 from Novagen

Description: Schistosomal glutathione-S-transferase (GST) is commonly used as a fusion partner when expressing proteins in E. coli (1). The GSTTag sequence has been reported to enhance the production and in some cases the solubility of its fusion partners. When expressed in a soluble, properly folded form, GSTT...
Company:Novagen

pET GST Fusion System 42 plus Competent Cells from Novagen

Description: Schistosomal glutathione-S-transferase (GST) is commonly used as a fusion partner when expressing proteins in E. coli (1). The GSTTag sequence has been reported to enhance the production and in some cases the solubility of its fusion partners. When expressed in a soluble, properly folded form, GSTT...
Company:Novagen

Mouse Anti-Mouse Natural Killer Cell Receptor-P1 Monoclonal Antibody, Unconjugated, Clone 1C10 from CHEMICON

Description:... Anti-Natural Killer Cell Receptor-P1, clone 1C10 Immunogen: Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized (BALB/c X C57BL/6)F1 (CbyB6F1) lymphokine-activated killer (LAK) cells and mouse myeloma cellline. Availa...
Company:CHEMICON

Mouse Anti-Escherichia coli Lac1 Monoclonal Antibody, Unconjugated, Clone 0.T.81 from Abcam

Description:... Mouse monoclonal [0.T.81] to Lac1 ( Abpromise for all tested applications). Antigen: Recombinant TrpE fusion protein containing full length E. coli Lac1....
Company:Abcam

Origami(DE3) Glycerol Stock from Novagen

Description:...els were similar (1). Origami hosts are compatible with ampicillin-resistant plasmids and are ideal for use with pET-32 vectors, since the thioredoxin fusion tag further enhances the formation of disulfide bonds in the cytoplasm. The Origami(DE3)pLacI host is compatible with expression from pETBlue and pTri...
Company:Novagen

Origami(DE3)pLysS Glycerol Stock from Novagen

Description:...els were similar (1). Origami hosts are compatible with ampicillin-resistant plasmids and are ideal for use with pET-32 vectors, since the thioredoxin fusion tag further enhances the formation of disulfide bonds in the cytoplasm. The Origami(DE3)pLacI host is compatible with expression from pETBlue and pTri...
Company:Novagen

Chicken Anti-DCTD (dCMP deaminase) Polyclonal Antibody, Unconjugated from GenWay Biotech, Inc.

Description:...ary Cancer; Gonad; Fetal S Applications Not Tested: ELISA, Immunocytochemistry, Immunohistochemistry Testing Protocols: Western Blot: E coli-derived fusion protein as test antigen. Affinity-purified IgY dilution: 1:2000, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development.||15-2...
Company:GenWay Biotech, Inc.

Mouse Anti-Bovine CD335 Monoclonal Antibody, ALEXA FLUOR 488 Conjugated, Clone AKS1 from AbD Serotec

Description:... MOUSE ANTI BOVINE CD335:ALEXA 488 Immunogen: fusion protein consisting of the extracellular region of bovine CD335....
Company:AbD Serotec

Mouse Anti-PLC delta-1 Monoclonal Antibody, Unconjugated, Clone d1-174 from Upstate

Description:... Anti-PLCδ-1, clone d1-174 GenBank Accession Number : NM_006225 Immunogen : His6 and X-press N-terminal tagged fusion protein corresponding to full length rat PLC delta 1 Formulation: 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of gly...
Company:Upstate

Mouse Anti-Rat WAVE 1 Monoclonal Antibody, Unconjugated, Clone 1.T.4 from Abcam

Description:... Mouse monoclonal [1.T.4] to WAVE 1 ( Abpromise for all tested applications). Antigen: His6 fusion protein corresponding to full-length rat WAVE1. Entrez Gene ID: 8936...
Company:Abcam

Rat Anti-HP1 gamma Monoclonal Antibody, Unconjugated, Clone MAC385 from ABR-Affinity BioReagents

Description:... Immunogen: Full length M32 fusion protein. Storage: -20 C, Avoid Freeze/Thaw Cycles...
Company:ABR-Affinity BioReagents

Calmodulin Sepharose 4B from GE Healthcare, formerly Amersham Biosciences

Description:... Calmodulin Sepharose 4B, 10 ml. For single-step purification of calmodulin-binding peptide fusion proteins.Purification of calmodulin-regulated proteins from all eukaryotic cells. Category: Chromatography Columns & Media, Affinity, Group Specific A...
Company:GE Healthcare, formerly Amersham Biosciences

Rabbit Anti-Bovine Respiratory Syncytial Virus Bovine Respiratory Syncytial Virus Antibody, Unconjugated from ABR-Affinity BioReagents

Description:... Immunogen: Human Respiratory Syncytial Virus (RSV) and Bovine Respiratory Syncytial Virus (BRSV) fusion protein Storage: -20 C, Avoid Freeze/Thaw Cycles...
Company:ABR-Affinity BioReagents

StrepMAB-Immo coated microplate; 96 well from IBA GmbH

Description:... The new StrepMAB-Immo coated microplates can be used for efficient, mild and oriented immobilization of SerAla-Strep-tag II fusion proteins for ELISA or other assays for protein analysis. Also small amounts of such proteins are bound with high efficiency to the microplate (see gra...
Company:IBA GmbH

Rabbit Anti-Human ASIP / PAR3 Monoclonal Antibody,, Clone ZMD.283 from Invitrogen

Description:... Clone/PAD: ZMD.283. Immunogen: fusion protein derived from human ASIP/PAR-3. Specificity: Specific for ASIP/PAR-3 (atypical PKC isotype-specific interacting protein/ partitioning defectiv...
Company:Invitrogen

Goat Anti-PAX3 Polyclonal Antibody, Unconjugated from GeneTex

Description:... with Waardenburg syndrome, craniofacial-deafness-hand syndrome, and alveolar rhabdomyosarcoma. The translocation t(2;13)(q35;q14), which represents a fusion between PAX3 and the forkhead gene, is a frequent finding in alveolar rhabdomyosarcoma. Alternative splicing results in transcripts encoding isoforms ...
Company:GeneTex

Rabbit Anti-JNK3 / SAPK1b Monoclonal Antibody, Unconjugated, Clone C05T from Upstate

Description:... Immunogen GST fusion protein corresponding to full-length rat JNK3/ SAPK1b. Quality Assurance Routinely evaluated by immunoblot. Stability 2 years at -20°C from d...
Company:Upstate

Mouse Anti-PSD-95, PDZ Domain Monoclonal Antibody, Unconjugated, Clone K28/86.2 from Upstate

Description:... Anti-PSD-95, clone K28/86.2, PDZ Domain GenBank Accession Number : NM_007864 Immunogen : GST fusion to residues 77-299 of human-PSD-95 (PDZ Domain) Formulation: 0.01M Tris pH 7.4, 0.075M NaCl containing 0.05% sodium azide Quality Assurance: routinely...
Company:Upstate

Strep•Tag® II Perfect Protein™ Markers, 16–100 kDa, Lyophilized from Novagen

Description:... These protein size markers are a mixture of Strep•Tag® II fusion proteins ranging from 16 to 100 kDa. The markers provide precise size references for proteins on SDS-polyacrylamide gels after staining with Coomassie...
Company:Novagen

pAcHLT-A-BFP from BD Biosciences Pharmingen

Description:...and a multiple cloning site (MCS). Foreign genes are expressed as BFP-6xHis fusion proteins when cloned into one of the available restriction enzyme sites (Ec...gl II). The presence of the BFP coding sequence allows the visualization of fusion proteins....
Company:BD Biosciences Pharmingen

pBICEP-CMV-3 Expression Vector from Sigma-Aldrich

Description:... For transient, cytoplasmic expression of an N-terminal FLAG® fusion and a second gene of interest or selection marker from bicistronic mRNA. Two genes can be cloned into MCS1 and MCS2 for transcription of a single mes...
Company:Sigma-Aldrich

Rabbit Anti-Mi-2 Polyclonal Antibody, Unconjugated from Upstate

Description:... Anti-Mi-2 Immunogen : fusion protein corresponding to amino acid residues 474-970 of human Mi-2 Formulation: 0.7M Tris-glycine, pH 7.4, 0.105M NaCl, 0.035% sodium azide, containin...
Company:Upstate

Mouse Anti-AP-2 alpha Monoclonal Antibody, Unconjugated, Clone 8G8/5 from Upstate

Description:... Anti-AP-2 α, clone 8G8/5 GenBank Accession Number : NM_002542 Immunogen : Bacterially made human AP-2alpha/gamma fusion protein corresponding to residues 166-223 of human AP-2α and residues 278-450 of human AP-2γ Formulation: 0.1M Tris-glycine, pH 7.4, 0.15M...
Company:Upstate

LIC Duet™ Trx•Tag™ Ek Adaptor from Novagen

Description:...rbs), ATG translation initation codon, and an N-terminal Trx•Tag coding sequence. The Trx•Tag is a 109 aa sequence that encodes thioredoxin. fusion to this sequence has been demonstrated to enhance solubility of many proteins that are typically insoluble in E. coli ...
Company:Novagen

LIC Duet™ GST•Tag™ Ek Adaptor from Novagen

Description:... reported to enhance the production and in some cases the solubility of its fusion partners. The GST•Tag is a multi-purpose tag that can be used for purification, quantification, and detection of fusion protein with the following Novagen products respectively: GST•Bind&tra...
Company:Novagen

LIC Duet™ Minimal Adaptor from Novagen

Description:...me binding site (rbs), and an ATG translation initation codon. This adaptor is designed for expression of a target protein with minimal vector-encoded fusion sequences....
Company:Novagen

Thrombin from Novagen

Description:...eparation is functionally tested for activity with fusion proteins and is free of detectable contaminating p...d as the amount of enzyme needed to cleave 1 mg of fusion protein in 16 hours at 20°C in a 200 µl ...l pH 8.4, 150 mM NaCl, 2.5 mM CaCl 2 , 50 µg fusion protein....
Company:Novagen

Rabbit Anti-Human sPLA2-XII Polyclonal Antibody, Unconjugated from BioVendor Laboratory Medicine, Inc.

Description:... sPLA2-XII Human, Rabbit Polyconal Antibody Anti-Human sPLA2-XII Antibody was raised in rabbits against Human sPLA2-XII His-Tagged fusion Protein Antigen: The recombinant human protein sPLA-XII. Source of Antigen: E. coli...
Company:BioVendor Laboratory Medicine, Inc.

Enterokinase from Novagen

Description:...t activity available, and is qualified for specific cleavage of appropriate fusion proteins. Supplied as a solution in 50% glycerol. EC 3.4.21.9, M.W. 26,300....one unit is defined as the amount of enzyme that will cleave 50 µg of fusion protein in 16 hours at 23°C, in a buffer containing 20 mM Tris-HCl pH 7...
Company:Novagen

Enterokinase from bovine intestine from Sigma-Aldrich

Description:... Physical Form: powder Color: white Removes FLAG peptide from N-terminal and Met-N-terminal fusion proteins. Supplied with optimized enterokinase buffer....
Company:Sigma-Aldrich

AcTEV Protease from Invitrogen

Description:...ving between Gln and Gly), making it useful for removing affinity tags from fusion proteins (1,2). For proper cleavage, the protein of interest is expressed w...nt incubation with AcTEV Protease releases the protein of interest from the fusion tag. This is an effective way to remove solubility, secretion, detection, a...
Company:Invitrogen

Rabbit Anti-M13 Genomic DNA Polyclonal Antibody, Unconjugated from Aviva Systems Biology

Description:...emid-based systems (1), rather than the phage-based system utilized in the Ph.D. libraries. Anti-M13 pIII Monoclonal Antibody can be used to determine fusion protein expression levels both in E. coli and on the M13 virion by immunoblot analysis. Reconstitution and Storage: Antibody is lyophilized in PBS bu...
Company:Aviva Systems Biology

HRV 3C Protease from Novagen

Description:...° C, high specificity, and His•Tag ® fusion make HRV 3C protease an ideal choice for rapid removal of fusion tags. The pET expression vectors pET-47b(+) throug...50b(+) vectors for expression, HRV 3C protease for fusion tag cleavage, and Ni-NTA His•Bind ® meta...
Company:Novagen

Enterokinase from Novagen

Description:...t activity available, and is qualified for specific cleavage of appropriate fusion proteins. Supplied as a solution in 50% glycerol. EC 3.4.21.9, M.W. 26,300. One unit is defined as the amount of enzyme that will cleave 50 g of fusion protein in 16 hours at 23C, in a buffer containing 20 mM Tris-HCl pH 7.4, 5...
Company:Novagen

Enterokinase EKMax from Invitrogen

Description:...ration (2). It recognizes the sequence Asp-Asp-Asp-Asp-Lys and cleaves the peptide bond after the lysine residue. The enzyme can be used to cleave any fusion protein that carries this peptide sequence....
Company:Invitrogen

GrabIt™ Protein G Plus/Protein A Kit from Novagen

Description:...nteractions from eukaryotic cells using a pull-down method. Proteins that interact with GST•Tag™, S•Tag™, or T7•Tag ® fusion proteins are copurified using affinity resins for the tags, and the complexes are retained on a spin filter. The complexes are eluted with sample buff...
Company:Novagen

Mouse Anti-PRMT5 Monoclonal Antibody, Unconjugated, Clone PRMT5-21 from GeneTex

Description:...n developed. These antibodies can be utilized to explore arginine methylation and its regulatory functions. Immunogen: Antibody was produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with PRMT5 containing the C-terminal region of the Human PRMT5 (amino acids 315-637)....
Company:GeneTex

IMPACT-CN System from New England Biolabs

Description:...his system distinguishes itself from other protein fusion systems by its ability to separate a recombinant p...ut the use of a protease. The IMPACT-CN Protein fusion and Purification System contains four expression vectors (pTYB vectors) which allow fusion of a bifunctional tag, consisting of the intein an...
Company:New England Biolabs
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