GELase LMP Agarose Gel-Digesting Preparation from EPICENTRE Biotechnologies
Description:GELase Agarose Gel-Digesting Preparation contains a unique β-agarose digesting enzyme developed at EPICENTRE for simple, quantitative recovery of intact DNA and RNA from low melting point (LMP) agarose gels following electrophoresis in TAE, TBE, MOPS, or phosphate buffers. The gel may be digested directly in the TAE, TBE, MOPS, and phosphate electrophoresis buffers or GELase Buff...GELase LMP Agarose Gel-Digesting Preparation from EPICENTRE Biotechnologies
Description:GELase Agarose Gel-Digesting Preparation contains a unique β-agarose digesting enzyme developed at EPICENTRE for simple, quantitative recovery of intact DNA and RNA from low melting point (LMP) agarose gels following electrophoresis in TAE, TBE, MOPS, or phosphate buffers. The gel may be digested directly in the TAE, TBE, MOPS, and phosphate electrophoresis buffers or GELase Buff...Vaccinia DNA Topoisomerase I from EPICENTRE Biotechnologies
Description:Topoisomerase I from vaccinia virus is a type I eukaryotic topoisomerase that removes both positive and negative superhelical turns (also called right- and left-handed supercoils) from covalently closed DNA. The product of the reaction is a covalently closed, circular DNA with fewer positive or negative superhelical turns. DNA Topoisomerase I does not absolutely require Mg to functio...Vaccinia DNA Topoisomerase I from EPICENTRE Biotechnologies
Description:Topoisomerase I from vaccinia virus is a type I eukaryotic topoisomerase that removes both positive and negative superhelical turns (also called right- and left-handed supercoils) from covalently closed DNA. The product of the reaction is a covalently closed, circular DNA with fewer positive or negative superhelical turns. DNA Topoisomerase I does not absolutely require Mg to functio...GELase LMT Agarose Gel-Digesting Preparation from EPICENTRE Biotechnologies
Description:GELase Agarose Gel-Digesting Preparation contains a unique β-agarose digesting enzyme developed at EPICENTRE for simple, quantitative recovery of intact DNA and RNA from low melting point (LMP) agarose gels following electrophoresis in TAE, TBE, MOPS, or phosphate buffers. The gel may be digested directly in the TAE, TBE, MOPS, and phosphate electrophoresis buffers or GELase Buff...GELase LMT Agarose Gel-Digesting Preparation from EPICENTRE Biotechnologies
Description:GELase Agarose Gel-Digesting Preparation contains a unique β-agarose digesting enzyme developed at EPICENTRE for simple, quantitative recovery of intact DNA and RNA from low melting point (LMP) agarose gels following electrophoresis in TAE, TBE, MOPS, or phosphate buffers. The gel may be digested directly in the TAE, TBE, MOPS, and phosphate electrophoresis buffers or GELase Buff...GELase LMT Agarose Gel-Digesting Preparation from EPICENTRE Biotechnologies
Description:GELase Agarose Gel-Digesting Preparation contains a unique β-agarose digesting enzyme developed at EPICENTRE for simple, quantitative recovery of intact DNA and RNA from low melting point (LMP) agarose gels following electrophoresis in TAE, TBE, MOPS, or phosphate buffers. The gel may be digested directly in the TAE, TBE, MOPS, and phosphate electrophoresis buffers or GELase Buff...EZ-Tn5 Transposase from EPICENTRE Biotechnologies
Description:EZ-Tn5 Transposase is designed for use with our pMOD series of vectors as well as any EZ-Tn5 transposon system....DNA Exonuclease III , E. coli from EPICENTRE Biotechnologies
Description:Exonuclease III digests duplex DNA in a 3'-> 5' direction from a blunt end, 5'-overhang or nick, producing stretches of single-stranded DNA. Under defined reaction conditions, DNA degradation by Exonuclease III proceeds at a uniform rate yielding predictable and reproducible results. Because the rate of exonucleolytic excision of deoxyribonucleotides by Exonuclease III is dependent upon reaction...DNA Polymerase I, E. coli from EPICENTRE Biotechnologies
Description:DNA Polymerase I from E. coli is a DNA-dependent DNA polymerase. The enzyme also contains both 5'--3'and 3'--5' exonuclease activities.1 The 5'?3' exonuclease activity enables the enzyme to use nicks and gaps in the DNA as starting points for labeling the DNA by nick translation....T4 Polynucleotide Kinase from EPICENTRE Biotechnologies
Description:T4 Polynucleotide Kinase (PNK) catalyzes the transfer of the gamma-phosphate from ATP to the 5'-hydroxyl of single- and double-stranded DNA, RNA, and nucleoside 3'-monophosphates. The enzyme also removes the 3'-phosphate from 3'-phosphoryl polynucleotides, deoxyribonucleoside 3'-monophosphates, and deoxyribonucleoside 3', 5'-diphosphates to form a 3'-hydroxyl group. Storage Buffer: 50% glycerol c...RNA Cap Analog, Trimethylated from EPICENTRE Biotechnologies
Description:A modified nucleotide structure called a ?cap? is present on the 5?-end of most naturally-occurring eukaryotic mRNAs and many viral RNAs. Substitution of Cap Analog for a portion of the GTP present in an in vitro transcription reaction results in the synthesis of transcripts with a cap on the 5'-end of the RNA...DHFR-1 FP-1 Forward Sequencing Primer from EPICENTRE Biotechnologies
Description:Contained in EZ-Tn5 Kits and are available separately for sequencing of an insertion site....RNA Cap Analog, Unmethylated from EPICENTRE Biotechnologies
Description:A modified nucleotide structure called a ?cap? is present on the 5?-end of most naturally-occurring eukaryotic mRNAs and many viral RNAs. Substitution of Cap Analog for a portion of the GTP present in an in vitro transcription reaction results in the synthesis of transcripts with a cap on the 5'-end of the RNA...RNA Cap Analog, Methylated from EPICENTRE Biotechnologies
Description:A modified nucleotide structure called a ?cap? is present on the 5?-end of most naturally-occurring eukaryotic mRNAs and many viral RNAs. Substitution of Cap Analog for a portion of the GTP present in an in vitro transcription reaction results in the synthesis of transcripts with a cap on the 5'-end of the RNA...ScriptCap m7G Capping System from EPICENTRE Biotechnologies
Description:ScriptCap m7G Capping system features the Vaccinia capping enzyme and all of the components needed to produce a Cap 0 on the end of di- or triphosphate RNA transcripts. These kits feature high efficiency capping (virtually 100%) at a significantly better price than competitive capping enzymes....TypeOne Restriction Inhibitor from EPICENTRE Biotechnologies
Description:DNA transformation can be difficult to achieve in many bacterial strains due to the presence of one or more restriction and modification (R-M) systems that cleave unmodified DNA that is recognized as "foreign". TypeOne Restriction Inhibitor provides a powerful method for increasing transformation efficiencies in bacterial strains with type I R-M systems. Developed as a unique preparation o...Mu-End Protein Truncation Kit from EPICENTRE Biotechnologies
Description:The Mu-End Protein Truncation Kit provides a convenient method for generating a library of N-terminal and C-terminal protein deletions that can be expressed in E. coli....MasterAmp 10X PCR Enhancer from EPICENTRE Biotechnologies
Description:EPICENTREs patented MasterAmp 10X PCR Enhancer (with betaine) substantially improves PCR performance and allows improved sensitivity and specificity for amplification of many DNA templates. Using MasterAmp PCR Enhancer in PCR eliminates the base-pair composition dependence of DNA melting, increases enzyme?s thermal stability, suppresses pauses of DNA polymerase, and reduces secondary DNA st...MasterAmp 10X PCR Enhancer from EPICENTRE Biotechnologies
Description:EPICENTREs patented MasterAmp 10X PCR Enhancer (with betaine) substantially improves PCR performance and allows improved sensitivity and specificity for amplification of many DNA templates. Using MasterAmp PCR Enhancer in PCR eliminates the base-pair composition dependence of DNA melting, increases enzyme?s thermal stability, suppresses pauses of DNA polymerase, and reduces secondary...CopyControl BAC Cloning Kit (EcoR I) from EPICENTRE Biotechnologies
Description:The CopyControl vector can be induced prior to DNA purification increasing DNA yields. These vectors provide the stability of single-copy BACs with the high yields associated with cosmids or other cloning vectors. The kits provide the linearized vector and associated reagents (ligation reagents, insert size screening system) necessary to create a 10X human BAC library. Speeds the library con...CopyControl BAC Cloning Kit (BamH I) from EPICENTRE Biotechnologies
Description:The CopyControl vector can be induced prior to DNA purification increasing DNA yields. These vectors provide the stability of single-copy BACs with the high yields associated with cosmids or other cloning vectors. The kits provide the linearized vector and associated reagents (ligation reagents, insert size screening system) necessary to create a 10X human BAC library. Speeds the library con...CopyControl BAC Cloning Kit (Hind III) from EPICENTRE Biotechnologies
Description:The CopyControl vector can be induced prior to DNA purification increasing DNA yields. These vectors provide the stability of single-copy BACs with the high yields associated with cosmids or other cloning vectors. The kits provide the linearized vector and associated reagents (ligation reagents, insert size screening system) necessary to create a 10X human BAC library. Speeds the library con...MaxPlax Lambda Packaging Extract from EPICENTRE Biotechnologies
Description:Extremely high efficiency packaging extracts useful for transfecting our EpiFOS or CopyControl Fosmids, or any DNA vector with cos sites. A tremendous value when conpared to the competition....FailSafe PCR System w/choice of 8 PreMix Tubes from EPICENTRE Biotechnologies
Description:The FailSafe PCR System is the only PCR system that ensures successful PCR, the first time and every time. It provides accuracy, consistency, and dependability for PCR, regardless of the source or the property of the DNA templates. This system uses EPICENTRE's FailSafe PCR Enzyme Mix, a unique blend of thermostable enzymes that is capable of amplifying the most difficult DNA templates...MasterAmp Taq DNA Polymerase Kit from EPICENTRE Biotechnologies
Description:MasterAmp Taq DNA Polymerase is a thermostable DNA polymerase derived from Thermus aquaticus. Having optimal activity at temperatures above 70°C, it was the first thermostable enzyme used for PCR and remains the predominant enzyme for that purpose. The enzyme has an intrinsic 5'?3' structure-dependent exonuclease activity, but lacks 3'--5' proofreading exonuclease activity. MasterAmp T...MasterAmp PCR Optimization Kit with (NH4)2SO4 from EPICENTRE Biotechnologies
Description:MasterAmp PCR Optimization Kits with ammonium sulfate are compatible with thermostable enzymes frequently used in PCR, such as Tfl, Tth, and Pfu DNA polymerases. You can significantly improve the performance of PCR systems in one experiment by adding your own enzyme, template, and PCR primers into the PreMixes supplied with the kit. This capability is made possible by the patented PCR Enhan...FailSafe PCR PreMix Selection Kit from EPICENTRE Biotechnologies
Description:The FailSafe PCR System is the only PCR system that ensures successful PCR, the first time and every time. It provides accuracy, consistency, and dependability for PCR, regardless of the source or the property of the DNA templates. This system uses EPICENTRE's FailSafe PCR Enzyme Mix, a unique blend of thermostable enzymes that is capable of amplifying the most difficult DNA templates...MasterAmp PCR Optimization Kit without (NH4)2SO4 from EPICENTRE Biotechnologies
Description:MasterAmp PCR Optimization Kits without ammonium sulfate are compatible with thermostable enzymes frequently used in PCR, such as Taq and AmpliThem DNA polymerases. You can significantly improve the performance of PCR systems in one experiment by adding your own enzyme, template, and PCR primers into the PreMixes supplied with the kit. This capability is made possible by the pat...MasterAmp PCR Optimization Kit without (NH4)2SO4 from EPICENTRE Biotechnologies
Description:MasterAmp PCR Optimization Kits without ammonium sulfate are compatible with thermostable enzymes frequently used in PCR, such as Taq and AmpliThem DNA polymerases. You can significantly improve the performance of PCR systems in one experiment by adding your own enzyme, template, and PCR primers into the PreMixes supplied with the kit. This capability is made possible by the pat...FailSafe PCR System w/choice of two PreMix Tubes from EPICENTRE Biotechnologies
Description:The FailSafe PCR System is the only PCR system that ensures successful PCR, the first time and every time. It provides accuracy, consistency, and dependability for PCR, regardless of the source or the property of the DNA templates. This system uses EPICENTRE's FailSafe PCR Enzyme Mix, a unique blend of thermostable enzymes that is capable of amplifying the most difficult DNA templates...pWEB::TNC Deletion Cosmid Transposition Kit from EPICENTRE Biotechnologies
Description:The pWEB::TNC Deletion Cosmid Cloning Kit enables you to prepare, in about two days, a complete and unbiased primary cosmid library of genomic DNA for subsequent use in making nested deletion sublibraries. High efficiency cosmid library construction is accomplished using easy and highly reproducible kit protocols. First, genomic DNA is randomly sheared by passing it through a syringe needl...Lambda GT11 Reverse DNA Sequencing Primer from EPICENTRE Biotechnologies
Description:These primers are designed for sequencing inserts cloned into M13/pUC-based plasmids, plasmids containing the SP6, T7, or T3 RNA polymerase promoters, or lambda gt10 and gt11 vectors. They are supplied at a concentration of 0.1 µg/µl in water. The primers are functionally tested in DNA sequencing reactions using SequiTherm EXCEL II DNA Polymerase with an end-labeled primer p...Lambda GT11 Forward DNA Sequencing Primer from EPICENTRE Biotechnologies
Description:These primers are designed for sequencing inserts cloned into M13/pUC-based plasmids, plasmids containing the SP6, T7, or T3 RNA polymerase promoters, or lambda gt10 and gt11 vectors. They are supplied at a concentration of 0.1 µg/µl in water. The primers are functionally tested in DNA sequencing reactions using SequiTherm EXCEL II DNA Polymerase with an end-labeled primer p...pWEB Cosmid Cloning Kit from EPICENTRE Biotechnologies
Description:...EZ-Tn5 Plasmid-Based Deletion Machine from EPICENTRE Biotechnologies
Description:The EZ-Tn5 Plasmid-Based Deletion Machine is based on the simple, highefficiency Tn5 in vitro transposition system developed by Goryshin and Reznikoff, and by York et al. for generation of nested families of deletions and inversions by intramolecular transposition into DNA targets cloned in a transposon-containing plasmid vector. Although most researchers will be interested in using the sys...2-Fluorine-CTP (2-F-CTP) from EPICENTRE Biotechnologies
Description:2'-Fluorine-CTP (2'-F-CTP) is readily incorporated into transcripts by T7 and SP6 R&DNA Polymerase and is available separately....FailSafe PCR 2X PreMix B from EPICENTRE Biotechnologies
Description:1, 2 or 8 PreMixes are included as part of the FailSafe PCR System....FailSafe PCR 2X PreMix E from EPICENTRE Biotechnologies
Description:1, 2 or 8 PreMixes are included as part of the FailSafe PCR System....mScript mRNA Production System from EPICENTRE Biotechnologies
Description:Create 100% capped transcripts with the translation boosting Cap 1 structure. mScript offers higher yields of 100% capped (versus 40% to 80% capped using co-transcriptional systems) eukaryotic mRNA messages in about 2 hours. mScript also features the Cap 1 structure, which has been shown to be up to 50% more efficient in in vivo tranlsation studies. mScript is the only commecially...