vivo in Biological News

Ex vivo neural stem cell expansion

In the March 1 issue of G&D, Dr. Christopher Fasano (Memorial Sloan-Kettering Cancer Center) and colleagues lend new mechanistic insight into the effective generation of neural stem cells outside of the neurogenic niche. Their upcoming paper demonstrates that the polycomb group protein, Bmi-1, ...

NIH grants Phylonix Phase II SBIR to develop high-throughput in vivo zebrafish assays

Cambridge, MA January 7, 2009 Phylonix Pharmaceuticals, Inc. today announced that it has been awarded a $1,250,369 Phase II Small Business Innovation Research Grant (SBIR) from the National Institute of General Medical Sciences of the National Institutes of Health (NIH) to develop in vivo zebra...

Researchers create new class of fluorescent dyes to detect reactive oxygen species in vivo

Researchers have created a new family of fluorescent probes called hydrocyanines that can be used to detect and measure the presence of reactive oxygen species. Reactive oxygen species are highly reactive metabolites of oxygen that have been implicated in a variety of inflammatory diseases, includ...

Pure carbon nanotubes pass first in vivo test

In the first experiments of their kind, researchers at Rice University and The University of Texas M. D. Anderson Cancer Center have determined that carbon nanotubes injected directly into the bloodstream of research lab animals cause no immediate adverse health effects and circulate for more than ...

Using nanoparticles, in vivo gene therapy activates brain stem cells

Using customized nanoparticles that they developed, University at Buffalo scientists have for the first time delivered genes into the brains of living mice with an efficiency that is similar to, or better than, viral vectors and with no observable toxic effect, according to a paper published this w...

Brain difference in psychopaths identified

... is called the uncinate fasciculus (UF). However, nobody had ever studied the UF in psychopaths. The team from King's used an imaging method called in vivo diffusion tensor magnetic resonance imaging (DT-MRI) tractography to analyse the UF in psychopaths. They found a significant reduction in the integ...

Scientists track impact of DNA damage in the developing brain

...ay and blocked the cells from completing the cell cycle. "The cells appear to undergo permanent arrest," said McKinnon, noting it is one of the few in vivo examples of the p53 pathway leading to cell cycle arrest rather than apoptosis. In the hippocampus, which plays a role in memory and emotion, inves...

U of M study finds new insight on therapy for a devastating parasitic disease

...iquantel within a flatworm model. "Our discovery of this new biological activity of praziquantel provides a foundation for defining the relevant in vivo targets of a very important clinical drug," said Jonathan Marchant, M.A. Ph.D., principal investigator of the study. "Using drugs to make organisms gr...

Prostate Cancer Translational Research in Europe meeting: Search for biomarkers continues

...lls. TSA, thiram, disalfiram and monensin (are) identified as cancer selective compounds inhibiting VCaP cell growth at nanomolar concentrations. In vivo studies using VCaP cell xenografts showed reduced tumour growth in response to disulfiram exposure; disulfiram was not able to block tumour growth ind...

Tips from the journals of the American Society for Microbiology

...y reduced replication of both viruses. "These results suggest a role for the IFN-a/β response in the control of H5N1 virus replication both in vivo and in vitro, and as such it may provide some degree of protection to the host in the early stages of infection," say the researchers. (K.J. Szrett...

Dual role in breast tissue for a protein involved in leukemia

...at5a collaborated with it to promote growth of a type of precancerous lesion of the breast termed a hyperplastic alveolar nodule. "Our studies in in vivo mouse models illustrate a dual role for the Stat5a protein in breast tissue. While it can contribute to the growth of one type of precancerous lesion ...

Universitat Autonoma de Barcelona researchers first to clone mice in Spain

...periments already pointed to improvements in the development of cloned embryos using inhibitors. However, scientists must wait until the end of the in vivo test period in July to obtain more conclusive data. ...

Versartis presents positive preclinical data on 2 product candidates at ENDO 09

... Jeffrey L. Cleland, Ph.D., Founder and Chief Executive Officer of Versartis. "Our preclinical data presented today show that VRS-317 demonstrated in vivo potency equal to daily hGH, and sustained plasma levels and pharmacodynamic responses in monkeys without toxicity. Potential benefits of VRS-317 incl...

CSIRO deal to commercialize 'artificial gut'

... new foods that is much quicker and more cost-effective than the current in vivo (human) method. The GI and RS Analyser is the first of its kind in the wo...uming process in the development phase of products, leaving the standard in vivo (human) test to the final stage of food development and labelling procedure...

Microtest Labs Adds Dissolution Testing to its Suite of Analytical and Stability Testing Services

...ormulations, different processes, analysis of competitor's samples) To estimate release and absorption rates in humans after an in vitro -- in vivo correlation is established Dissolution is a sample preparation technique. Samples still need to be analyzed separately, usually by M...

Vitamin D levels linked to asthma severity

...e previous in vitro studies have suggested that vitamin D may affect how airway cells respond to treatment with inhaled steroids, this is the first in vivo study of vitamin D and disease severity in children with asthma. Juan Celedn, M.D., Dr. P.H. and Augusto Litonjua, M.D., M.P.H. of Harvard Medical ...

Penn researchers demonstrate a new model for drug discovery with a fluorescent anesthetic

... the compound is fluorescent, researchers are able to image the compound in vivo in order to study its physiological effects. Where and how an anesthetic c..."1-AMA appears to be specific in its binding to proteins and also in its in vivo localization, which should give us the opportunity to determine its mechani...

Redefining what it means to be a prion

...ns several additional avenues of study, including the application of similar strategies to find additional yeast prions, thorough testing of prions in vivo and in vitro, and scanning of other organisms' genomes for prion candidates. ...

Basic guides to PCR, labeling neurons featured in Cold Spring Harbor Protocols

...available in the April issue. This patch-clamp methodology has been successful for single-cell dye labeling in cultured neurons, brain slices, and in vivo preparations. A wide range of dyes can be loaded using this method, including probes for morphological reconstruction, ion-sensitive indicator dyes f...

Brain building: Study shows brain growth tied to cell division in mouse embryos

...ution of a spindle-orienting factor that in normal cells distributes asymmetrically. Such similarities between Vangl2-lacking cells in vitro and in vivo will facilitate ongoing studies of the PCP pathway in neurogenesis. ...

Hormone-mimics in plastic water bottles -- just the tip of the iceberg?

...he researchers found evidence of estrogenic compounds leaching out of the plastic packaging into the water. What's more, these chemicals are potent in vivo and result in an increased development of embryos in the New Zealand mud snail. These findings, which show for the first time that substances leaching...

Phylonix to present at 2009 Society of Toxicology Annual Meeting

...lonix Pharmaceuticals, Inc., the leading contract research organization for preclinical testing in zebrafish, will present data about the company's in vivo zebrafish-based assays at the 48th Society of Toxicology (SOT) Annual Meeting being held March 15-19 at the Baltimore Convention Center in Baltimore, ...

GUMC young scientist selected postdoc Fellow at National Space Biomedical Research Institute

...research will investigate injury responses including inflammatory signaling induced by space radiation. The study will use a genetic approach in an in vivo model to investigate the effects of radiation-induced inflammatory signaling on cellular and molecular parameters in the intestine. Exposures, such a...

Rett Syndrome Research Trust advisor makes significant discovery

...he 2007 Science publication by Professor Adrian Bird showing reversal of Rett symptoms in adult mouse models of the disorder. Results from ongoing in vivo mouse studies deleting MeCP2 in glia are promising in terms of supporting the culture studies. "This new study adds to the growing body of evidenc...

New target for medicine to combat Alzheimer's: VIB scientists confirm protein's key role

...3 protein as a new player. The VIB researchers have been able to show − in vitro (in cells isolated and studied in the laboratory) as well as in vivo (in living animals − mice, in this instance) − that blocking GPR3 leads to reduction of the plaques. Importance of this research The...

Compounds could be new class of cancer drugs

...and Pharmacology, was elected to the National Academy of Sciences for his work on PLDs. The researchers will now optimize their new compounds for in vivo studies and to give them characteristics compatible with being good medications. They are also expanding their research into other areas of biology i...

Rice eyes risks of quantum dots

...ay be quickly weathered by acid rain, he said. Another concern is that acids in the body could break down dots used in such medical applications as in vivo imaging. "If they degrade faster than they can be excreted, there's the potential for heavy metals to be released into the body," said Alvarez. "Then ...

JCI online early table of contents: Jan. 19, 2009

...olleagues, at the University of Bristol, United Kingdom, have found that mice lacking the protein PKC-alpha exhibited diminished thrombus formation in vivo but showed no evidence of overt bleeding, they suggest that PKC-alpha is a potential target for antithrombotic therapy. In particular, they propose th...

Tips from the American Journal of Pathology

...ed white blood cell recruitment. Instead, annexin A1 colocalized with another FPR family member, FPR-2. The data by Gastardelo et al "provide in vivo evidence that endogenous annexin A1 is an essential mediator for homeostasis during the inflammatory process." They go on to propose "that these exper...

Apolipoprotein(a): A natural regulator of inflammation

...inogen leukocyte recruitment, evidence for this in vivo has been lacking. In vivo investigation of Lp(a) function has been impeded b...en interaction, inhibits neutrophil recruitment in vivo and functions as a cell specific suppressor of the...

New research lights up chronic bacterial infection inside bone

...teria. Their report published in the journal Disease Models & Mechanisms ( DMM ), dmm.biologists.org describes how they use a technique called in vivo bioluminescence to light up bacteria and allow them to see extremely tiny amounts of bacterial cells in living animals. Using this method, they found...

The medium is the message: Manipulating salmonella in spaceflight curtails infectiousness

...lla encounters just such conditions not only during spaceflight but also in vivo in an infected individual when the bacterium makes contact with an intestin...g hints, a full understanding of the complex interplay of forces and the in vivo mechanisms of Salmonella pathogenesis await further research. Fortunatel...

Studies on imaging and tracking transplanted cells

...ytes, the researchers for the first time imaged in vivo human hepatic progenitor cells transplanted into a... treated with SPIO-labeled MSC, indicating that in vivo the initially cell surface bound iron detached fro...tients in the clinical setting, indicating that in vivo application of SPIO-labeled MSC needs to be perfor...

Transplanted fat cells restore function after spinal cord injury

...nal cells in an in vitro setting. In their study, for the first time fat cells have been shown to successfully differentiate into neuronal cells in in vivo tests. The fat cells are grown under culture conditions that result in them becoming de-differentiated fat (DFAT) cells. "These cells, called DFAT...

Stowers Institute's Linheng Li Lab expands understanding of bone marrow stem cell niche

...Online Publication of Nature . The Linheng Li Lab collaborated with several of the Institute's support facilities to develop a new technology ex vivo Imaging of Stem Cells, or EVISC to monitor the dynamic behavior of stem cells. This technology enabled the team to track the homing of hematopoietic ...

Vitamin E shows possible promise in easing chronic inflammation

...tween short-term Vitamin E supplementation and reduced inflammation in the brain. The study represents the first time researchers have looked at in vivo effects of Vitamin E administration on local inflammatory responses in skeletal and cardiac muscle. In this study, the researchers investigated the...

Intraspinal implant of mesenchymal stem cells may not heal the demyelinated spinal cord

...r Brain Repair at the University of Cambridge. "A number of in vitro and in vivo studies have reported that MSCs have differentiated into neuronal cells and...ugh MSCs may possess neural differentiation capabilities in vitro, their in vivo behavior is unpredictable, said Dr. Hunt and his co-authors. However, they...

Scripps research scientists identify compounds for stem-cell production from adult cells

...eprogramming process, but also become useful in in vivo stem cell biology and, ultimately, the development...eral cells treated with BIX alone. In vitro and in vivo characterizations confirmed that transduction of o...hout impacting healthy cells systemically in an in vivo setting." ...

New journal shows half-broken gene is enough to cause cancer

...iting research on the molecular mechanisms underlying the manifestations of disease phenotype". PathoGenetics will focus on both in vitro and in vivo studies on the cascade of events leading from gene mutations or genomic rearrangement to disease. The discovery of novel molecular and metabolic pathw...

OSA's ISP launches with research on breathing disorders and congenital heart defects

...y heart development go to http://www.opticsinfobase.org/abstract.cfm?msid=99859 . A. M. Davis, F. G. Rothenberg, N. Shepherd, and J. A. Izatt, "In vivo spectral domain optical coherence tomography volumetric imaging and spectral Doppler velocimetry of early stage embryonic chicken heart development," ...