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gel in Biological Dictionary

Gel

A gel is a solid, jelly-like material that can have properties ranging from soft and weak to hard and tough. Gels are defined as a substantially dilute crosslinked system, which exhibits no flow when in the steady-state. By... Environmental laboratory, consulting, engineering, and geophysical ...

Polyacrylamide Gel

A Polyacrylamide Gel is a separation matrix used in electrophoresis of biomolecules, such as proteins or DNA fragments. Traditional DNA sequencing techniques such as Maxam-Gilbert or Sanger methods used polyacrylamide gels to separate DNA fragments differing by a single base-pair in lengt...

Native Gel

Native Gel Electrophoresis is a technique used mainly in protein electrophoresis where the proteins are not denatured and therefore separated based on their charge-to-mass ratio. Native polyacrylamide gel electrophoresis is an electrophoretic separation method typically used in prot...

Denaturing Gel

A denaturing gel is a type of electrophoresis in which the native structure of macromolecules that are run within the gel is not maintained. For instance, gels used in SDS-PAGE will unfold and denature the native structure of a protein. In contrast to Native Gel Electrophoresis, quat...

Agarose Gel

Agarose gel electrophoresis is a method used in biochemistry and molecular biology to separate DNA, or RNA molecules by size. This is achieved by moving negatively charged nucleic acid molecules through an agarose matrix with... ... necessary for conducting agarose gel electroph...

Xanthophyll

...ylls in the macula may indicate a protective role ... Analysis of the xanthophyll binding LHC proteins showed that the Lhcb5 ... from the IEF gel showed differences in xanthophyll composition: ... ...

Western blot

... The western blot is an analytical technique used to detect specific proteins in a given sample of tissue homogenate or extract. It uses gel electrophoresis to separate native or denatured proteins by the length of the polypeptide or by the 3-D structure of the protein .... Info...

Tube feet

...th numerous tube feet . ... canal: tube that forms a ring of a starfish. Tube feet : organ of ... Shop for Synergy Mission Product Foot gel Tube 2 Oz at mySimon. Smart Shopping at mySimon. Derma Athletes Foot Cure, Tube - by Quantum - available now at WebVitamins.com for only $8.8...

Starch

...nt in the seeds of cereal... This page is from http://food.oregonstate.edu / which generally contains images, ... explain the process of sol and gel formation in starch -water dispersions. ... Starch is a polysaccharide, a chain of many glucose molecules. ... In the starch grains in a pl...

Southern blotting

...t is a method routinely used in molecular biology for detection of a specific DNA sequence in DNA samples. Southern blotting combines agarose gel electrophoresis for size separation of DNA with methods to transfer the size-separated DNA to a filter... The Southern blot is used for DN...

Southern blot

...ot is a method routinely used in molecular biology for detection of a specific DNA sequence in DNA samples. Southern blotting combines agarose gel electrophoresis for size separation of DNA with methods to transfer the size-separated DNA to a filter... Professor Sir Edwin Mellor Southern ,...

Somatic hypermutation

...m inside cells that is part of the way the immune system adapts to the new foreign InVivoScribe Product - IGH Somatic Hypermutation Assay - gel Detection. Rearrangements of the antigen receptor genes occur during ontogeny in B and T lymphocytes. ... InVivoScribe Product Group - IGH S...

SDS-PAGE

...DS - PAGE , sodium dodecyl sulfate polyacrylamide gel electrophoresis, is a technique widely used in bi...phoretic mobility .The... SDS polyacrylamide gel electrophoresis ( ¦es¦dē¦es ′pälēə¦kriləmīd ′je...or sodium dodecyl (lauryl) sulfate-polyacrylamide gel electrophoresis. ... This is how SDS - PAGE sep...

SCAR

...e a story? How Do I Get a Scar ? ... Scar Less Generation III Topical gel helps beautify and fade active scars . ... SCAR LESS™ Generation III Topical gel even works to help prevent new scars from ... Offering an extensive co...

Revertant

...saicism occurs when an ... [ Revertant DNA data] (image 2) High resolution version (8,135,867 Bytes) Description: Varmus made extensive use of gel electrophoresis in his study of the genetics of ... Revertant mosaicism by somatic reversion of inherited mutations has been ... Revert...

RFLP

...rsity estimate for a particular ... Prepare a 3% NuSieve (Cambrex) agarose gel for RFLP . ... Perform PCR with Beckman D4-labeled (5'-end) forward 18...ngth polymorphism ( RFLP ) is most suited to studies at the ... Figure 1. gel showing RFLP variation between SCN and SBCN using Fok I RE ... ...

Primer extension

... Primer Extension . • Allele Specific Oligonucleotide Ligation. • Sequencing ... One can label the primer and apply the extension products to gel electrophoresis. ... Klenow fragment in primer extension avoids loss of incorporated label. ... PRINCIPLE OF RANDOM PRIMER EXTENSION ... f...

Polymerase

...que ... DreamTaq™ DNA Polymerase uses the same reaction set-up and cycling ... T4 DNA Polymerase . Genomic DNA Purification Kit. Silica Bead DNA gel Extraction Kit ... ,biological,biology definition,biology terminology,biology terms,biology abbreviations ... DNA polymerase occurs naturally in ...

PFGE

...ation including descriptions of FSIS, Pulsed Field gel Electrophoresis, PFGE Patterns, Pulse Net, and r...technique used in this discipline is pulsed-field gel electrophoresis ( PFGE ). PFGE is currently u...tial migration of large DNA ... Pulsed-field gel electrophoresis ( PFGE ) is one of the most wid...

Northern hybridization

...Temperature Hybridization Solution ... Northern blotting protocols ... Northern Hybridization of RNA Fractionated by Agarose-formaldehyde gel ... Northern Hybridization (Laurie Lab, University of ... ... the quantification of DIG-labeled RNA and as a hybridization . control ... Note:...

Lambda phage

..... lambda phage DNA is either linear, circular, or concatenated. ... Lambda phage concatemers are used as size markers in PFGE (Pulse Field gel Electrophoresis) ... Lambda Phage . World of Goo. January 18, 2009 by lambdaphage ... Lambda . ... Lambda . (photo © LambdaPhage) ... 2. Ev...

Hybridization

...SH), in its simplest form, can be used to identify as many labeled features as there are different fluorophores used ... 2. Hybridization in the gel or liquid 42. 3. Recombination in vitro 43 ... (1994) Production of DNA Hybridization Probes with Digoxigenin- Modified ... Procedures for In ...

His tag

..... ... Tag Purification. Affinity Tag Purificaton Columns. His Tag ... His - Tag Purification ColumnSpin FormatHIS-Buster Cobalt Affinity gel is a tetradentate... ... Kits " His Tag Purification Kits " His Tag Purification Kits ... Laboratory Supplies " Protein Biochemistry " Prot...

Heteroduplex analysis

...x analysis (HDA) 1 is a popular gel-based mutation scanning method ... gel analysis of titer experiments was performed in which heteroduplex forma...oven to be a robust tool for mutation detection. ... Background: Slab gel heteroduplex analysis (HDA), a ... Nonstandard abbreviations: HDA, he...

Genomic library

...raries . page 2 of 23. Medicago ... The first Mt genomic libraries were constructed in phage lambda derivatives in the 1990s. ... [While gel is running, you will be starting cDNA library construction] ... dephosphorylated (for details, see "Plasmid Library Construction" handout) ... ...

Gel shift assay

...ility shift electrophoresis, also referred as a gel shift assay , gel mobility shift assay , band shift assay , or gel retardation assay , is a common affinity electr...

Gel electrophoresis

... gel electrophoresis is a technique used for the sep... molecules using an electric current applied to a gel matrix. DNA gel electrophoresis is generally... Agarose ...

Ethidium Bromide

... Ethidium bromide is an intercalating agent commonly used as a fluorescent tag in molecular biology laboratories for techniques such as agarose gel electrophoresis. When exposed to ultraviolet light, it will fluoresce with an... ethidium bromide ( e′thidēəm ′brō′mīd ) ( organic chemistry )...

Electrophoresis

...n applied electric field. There are detailed... gel electrophoresis is a technique used for the sepa... molecules using an electric current applied to a gel matrix. DNA gel electrophoresis is generally... Sort and meas...

Dot blot

...ns, DNA or RNA, onto a carrier . In many instances, this is done after a gel electrophoresis, transferring the molecules from the gel onto the blotting membrane, and other times adding the samples directly ...

DNA sequence

...ets in the deconstruction of genes. An explanation of how DNA sequencing works. ... DNA sequencing is also dependent on our ability to use gel electrophoresis to ... ... available to determine the actual sequence of nucleotides in a segment of DNA . ... put through a series of DNA...

DGGE

... Temperature Gradient gel Electrophoresis (TGGE) and Denaturing Gradient gel Electrophoresis ( DGGE ) are forms of electrophor...d DGGE are useful for... Denaturing gradient gel electrophoresis ( DGGE ) is a commonly used tec...

Denature

... 4 and ... denature . Typical PCR reaction. Chimera formation via partial extensions. Assumptions of clone and ... DGGE - Denaturing gradient gel electrophoresis ... NOTE: To denature Labeled DNA for a hybridization application, please see ... Do not heat- denature the Labeled DNA pr...

Contig

...cloned deoxyribonucleic acid fragments from an A contig is a set of gel readings that are related to one another by overlap of their sequences. ... The gel readings in a contig can be summed to form a ... Contig Contig is...

Collagen

...llagen . Why collagen ? ... entirely Natural AntiAging SkinCare Collagen gel that is pure, ... Yes Natural Collagen Transdermal gel Collagen is specially formulated for care ... are not created using any t...

Biotechnology

...purest form, the term " biotechnology " refers to the use of living ... BIOZONE Biotechnology | Links include biotechnologigy techniques such as gel ... Applications in Biotechnology . • Cloning & Tissue Culture. • Genome Projects ... Definition of biotechnology and a description of the ...

Binding site

...tial Binding Site Search. TFD Search. Getting Started... Right below the binding site plot, there are color switches for you to turn on ... gel filtration data also suggested the presence of a third binding site of 5.4 kDa. The 170-kDa site had a binding affinity of Kd = 12 x 10-6 and ...

Bicuspid

...ness, and lifestyle destination... State audit cites Texas dental board shortcomings. A report released this week by the Texas State ... Aloe vera gel equal to toothpaste in battling caries ... Bicuspid - Definition of Bicuspid at Dictionary.com a free online dictionary ... Also called bicus...

Band shift assay

...ility shift electrophoresis, also referred as a gel shift assay , gel mobility shift assay , band shift assay , or gel retardation assay , is a common affinity electr...

Annulus

...tatus issue (and ... AnaLog Services, Inc. provides services to the oil well, water well, and mineral logging / wireline industries. ... Annulus gel was researched at EPA's Kerr ... Successful phase contrast microscopy requires utilization of the proper equipment (a condenser annulus and obj...
Other Tags
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