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Tag: "vitro" at biology news

New egg freezing technique offers hope to hundreds of women

...ough sperm could be frozen, thawed and used for in vitro fertilisation with high levels of success, the freeze-thawing process could damage eggs and, until now, it had been very difficult to perform successful IVF using frozen-thawed eggs. Research by Professor Stefania Nottola and Dr Sandrine Chamayou, pr...

Study finds that a woman's chances of having twins can be modified by diet

...e new study, Dr. Steinman confirmed that use of in vitro fertilization (IVF) methods increases the incidence of monozygotic twinning -- where the transfer and/or implantation of two embryos results in three infants -- and he proposed that adding more calcium or reducing the chelating agent ethylenediamine ...

PTEN: the Treg's handbrake

...on-regulatory T cells, Tregs do not proliferate in vitro when stimulated through either their TCR or IL-2 r...cient Tregs retained their suppressive function in vitro and in vivo, indicating that loss of PTEN by Tregs only affects their proliferative capabilities in ...

How IVF could be causing genetic errors in embryos

...h embryos are cultured in the laboratory during in vitro fertilisation could be causing genetic errors that... which showed no difference in expression after in vitro culture compared to the in vivo embryos. Five genes showed a statistical difference compared to the ...

Mass. General study finds potential ovarian cancer stem cells

...ere less responsive than regular tumor cells to in vitro treatment with the chemotherapy drug doxorubicin, the stem-like cells remained sensitive to repeated treatment with Mullerian Inhibiting Substance (MIS). This protein, important in the normal development of sexual organs, has been studied for its p...

Stanford snake venom study shows that certain cells may eliminate poison

...r venom. Galli and Metz first did experiments in vitro using isolated sarafotoxin 6b with mast cells from...me way mouse mast cells do. The group has begun in vitro studies using human mast cells to evaluate this possibility. ...

APS lecturer shows rare video of 'teacher-student' immune cell interactions in live animal

...tly view much of this complex cellular teamwork in vitro and in real time. The von Andrian videos provided a real-time look at what happens inside a mouse lymph node when a foreign microbe enters the body. The videos were made possible by recent technological advances together with techniques the von Andr...

Cyclosporine inhibits hepatitis C virus in vitro

...ed its effect on hepatitis C in vitro. For the in vitro study, the researchers, led by Roberto J. Firpi, M.D. of the University of Florida, treated the HCV replicon line, GSB1, with varying doses of Cyclosporine for 48 hours and examined the results. They found that Cyclosporine reduced HCV replication by...

U of S researchers develop new vaccine candidate against hepatitis C

...them. The researchers exposed dendritic cells in vitro to a HCV protein. The cells were also exposed to a strong immune stimulator to increase the immune response and then injected into mice as a vaccine. Because HCV does not infect mice, mice were challenged with a carrier virus containing the hepatitis...

Potential heart benefit found in stem cells

...that assists in intracellular interactions The in vitro data suggests that stem cells from this source have significant CM potential and are potentially valuable not only because they can be easily collected from young volunteers, but also because of their collection-efficacy. A single sample of the menst...

Absence of critical protein linked to infertility

...gnancy. "The success rate for the practice of in vitro fertilization currently is, on average, about 25 percent," she said. "The major problem is that the conditions occurring when the embryo is transferred often are not the best in the uterus. It's not known if the uterus is ready to accept an embryo, s...

Protein translation in sperm

...actin polymerization, the acrosome reaction and in vitro fertilization rates. Thus, protein translation in sperm is essential for sperm functions that directly contribute to fertilization. Dr. Breitbart is confident that "The new findings would give us better understanding for treatment of male infertili...

Marijuana-like compounds suppress the immune response

... the Week", Yumi Tohyama and colleagues used an in vitro model of blood cell migration to study the involvement of CB2 in the recruitment white blood cells. They found that treating the blood cells with compounds that bind to CB2 suppresses the migration of the cells. When they examined the cells, they d...

'Accelerated evolution' converts RNA enzyme to DNA enzyme in vitro

...verted to a corresponding deoxyribozyme through in vitro evolution. The ribozyme was first prepared as a DN... this DNA were prepared, and repeated cycles of in vitro evolution were carried out. The result was a deoxyribozyme with about the same level of catalytic ac...

Horse antibodies against the bird flu virus H5N1 are effective as treatment in mice

...institutions in China infected dog kidney cells in vitro with a lethal dose of H5N1 and simultaneously expo...antibodies to H5N1 protected cells against H5N1 in vitro ?the cells simultaneously infected with H5N1 and exposed to horse antibodies did not die. Lu et al...

Living taste cells produced outside the body

... demonstrated that taste cells can be generated in vitro and maintained for a prolonged period of time." T...r explains, "By producing new taste cells in an in vitro system, our results demonstrate that direct stimulation from nerves is not necessary to generate tas...

New evidence questions the simple link between prion proteins and madcow disease

...h whole animals as opposed to relying purely on in vitro studies, the more cautious you are about saying that prion proteins alone cause the disease," says Martin Jeffrey. In a commentary published in the same edition of the journal Dr Nicole Sales of the Department of Infectology, at the Scripps Researc...

Swiss researchers develop all-in-one remote control gene expression tool

...om HIV. When injected into living cells ?either in vitro or in vivo ?the genetic material aboard the lentiviral vector joins the genetic material in the nucleus of the cell, causing the cell to express the protein encoded by the new gene. This versatile package can also carry bits of RNA that stop the cell...

Cellular scale drug delivery from the inside out

...al containing human cervical cancer cells grown in vitro that contain nanospheres capped with iron-oxide particles. The cells slowly migrate and cluster to the side of the vial next to the magnet. "By using a powerful magnet, we can first concentrate the nanospheres at a particular point, such as a tumor...

Stem cells from muscles can repair cartilage

... study also included culturing MDSC and MDSC-B4 in vitro in three different types of media and found that MDSC-B4 were able to differentiate into chondrogenic cells (cells that develop into cartilage) depending on the type of medium in which they grew. The addition of transforming grown factor â1 (TGFâ1) t...

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