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Tag: "spectrometry" at biology news

Technique monitors thousands of molecules simultaneously

.... He praised the time-of-flight secondary-ion-mass spectrometry work of his Washington University colleague Amy Walker, Ph.D., assistant professor of chemistry, in the monitoring of his system. ...

Mine runoff continues to provide clues to microbial diversification

...lth and bioremediation.? The combination of mass spectrometry support from ORNL researchers with extensive recon...ooperate and compete in nature. In fact, ORNL mass spectrometry provided the ability to resolve and differentiate peptides that differ by as little as one amino aci...

Protein fragments sequenced in 68 million-year-old Tyrannosaurus rex

... the bones," said John Asara, director of the mass spectrometry core facility at Beth Israel Deaconess Medical Sch...e of a year and a half using highly sensitive mass spectrometry methods. "This allows you to get the chance to say, ‘Wait, they really are related because their seq...

Speeding 'fingertip' discovery -- 20 years of protein info in 1 place

...odifications like phosphorylation intact. Previous spectrometry methods were “just too rough?on the delicate protein alterations and sheared them right off, he says. “We had to guess where they might be and nobody wants to chase false leads based on wrong guesses.? Pandey says the original goal of the researc...

Tears reveal some of their deepest secrets to researchers

...ry. They used a technique called electrospray mass spectrometry to differentiate between the different lipid compo...s of lipids in the tear film, but advances in mass spectrometry technology are improving research efforts. "Other scientists used different techniques to try to ...

Proteins may predict lung transplant rejection

...eomics (the study of proteins), the latest in mass spectrometry technology and the best analytical methods from the field of bioinformatics (the use of computers and statistics to analyze and find patterns in scads of data). They hypothesized that proteins would change as chronic lung rejection developed and that...

Fantastic Voyage: A new nanoscale view of the biological world

...ribe the development of multi-isotope imaging mass spectrometry (MIMS), which has applications in all fields of biology and biomedical research. "This method allows us to see what has never been seen before, and to measure what has never before been measured," Lechene says. "Imagine looking into a building, sli...

New technique boosts size of proteins that can be analyzed

... in the Oct. 6 issue of the journal Science. "Mass spectrometry characterizes a protein by measuring the masses produced from it." The new technique provides a far more efficient way to break down proteins inside the mass spectrometer -- sprayed, heated and then bashed with gas molecules -- to obtain their piec...

High-throughput oncogene mutation detection in human cancers by mass spectrometry-based genotyping

...ver 92 percent of mutations identified by the mass spectrometry method were validated in this manner, and believe ...that much of the difference is due not to the mass spectrometry method but to limitations of the laborious sequencing test "which is not always sensitive in tumor s...

Plague proteome reveals proteins linked to infection

... techniques called accurate mass and time tag mass spectrometry and clustering analysis to compare abundance changes in 992 proteins under four different growth conditions, at 26 degrees and 37 degrees Celsius and with and without calcium. They found 89 candidate proteins with similar abundance changes to 29 k...

With record resolution and sensitivity, tool images how life organizes in a cell membrane

...ber and Ian Hutcheon, both experts in imaging mass spectrometry at the Lawrence Livermore National Laboratory (LLNL), who provided access to the state-of-the-art machine used in the experiments. That machine is a highly specialized mass spectrometer, which analyzes the mass of small molecular ions formed when a ...

Major cancer study aims to identify protein markers for early-stage disease

...k to establish the best method for conducting mass spectrometry in the context of cancer biomarker discovery. Mass spectrometry is a technique used to detect and measure the precise molecular weight of proteins. A critical secon...

High-tech research shows cocaine changes proteins and brain function

...f the proteins and then using high-throughput mass spectrometry which allowed the accurate identifcation of thousands of proteins simultaneously, Hemby said. The unbiased nature of the technology enables the determination of novel proteins and pathway involved in disease. Using post-mortem brain tissue samples...

Researchers find protein that silences genes

...eral different histones. A collaboration with mass spectrometry expert Michael Gross, Ph.D., Washington University professor of chemistry, helped define the precise locations of the acetyl groups that HDA6 can remove, down to which acetylated amino acids are involved. "The bottom line is that HDA6 has very bro...

Reversing 'hibernating' heart muscle focus of UB researchers

...-of-the art proteomic research facilities and mass spectrometry at UB. "By using therapies similar to those that are used clinically in patients, such as angioplasty and adenoviral gene transfer to over-express growth factors in the heart, we can identify how changes in the molecular pathways responsible for t...

Links between DNA damage and breast cancer studied

...cal analysis technique (liquid chromatography/mass spectrometry with isotope dilution) to identify specific DNA lesions, while the PNRI team used a spectroscopic technique (Fourier transform-infrared spectroscopy) to reveal subtle conformational changes to DNA base and backbone structures. Such alterations to the...

Precision biochemistry tracks DNA damage in fish

...g liquid and gas chromatography combined with mass spectrometry (LC/MS,GC/MS). Precision chemical analysis techniques at NIST allowed the researchers to identify and measure damage to adenine and guanine, specific chemical components or bases of DNA, at extraordinarily low levels--five lesions out of a 100 millio...

Ancient ants arose 140-168 million years ago

Scientists have used mass spectrometry for decades to determine the chemical composition ...c Health recently demonstrated that proteomic mass spectrometry has the potential to be applied for this purpose. Using a two-step process, researchers successfully...

Researchers use mass spectrometry to detect norovirus particles

Scientists have used mass spectrometry for decades to determine the chemical composition ...c Health recently demonstrated that proteomic mass spectrometry has the potential to be applied for this purpose. Using a two-step process, researchers successfully...

Salmonella caught red-handed

... Biochemistry then turned to highly-sensitive mass spectrometry to look at the protein mixture ?and discovered hundreds of different Salmonella metabolic path proteins. The scientists compared them with special protein databanks and identified possible points of attack for antibiotics. Bumann and his team then e...

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