Exploring the dark matter of the genome
...n are sequenced using a method called whole-genome shotgun sequencing. Celniker says, "We grind up whole flies and produce libraries of DNA fragments of two sizes, some that are 2 kilobases long" -- a kilobase (Kb) is 1,000 bases -- "and some that are 10 kilobases long. Contiguous lengths of sequence are ass......hes for studies of microbial communities." In a shotgun sequencing process, the DNA from the microbial genomes is first sheared into millions of small fragments to enable the amplification, labeling, and ultimately sequencing. Genome assembly is the process of putting the sequenced fragments back in orde...... indicates that the synapse may behave more like a shotgun than a rifle when it comes to firing the neurotran...ight now they have little idea of how the synaptic shotgun functions. The research was published in the July 15, 2005, issue of the journal Science. Sejnowski...