Researchers develop new method for facile identification of proteins in bacterial cells
...ch are scooped out of the gel and cut with protein scissors into predictable, small pieces called peptides. The samples are then analyzed using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), which identifies protein fragments based on the time they need to travel ...Twin molecular scissors link creation of microRNAs with gene-silencing
...bed. "The two enzymes in the complex are like two scissors working together in a concerted fashion, connected and coordinated by the third member of the complex," Shiekhattar explains. Another scientific question surrounding RISC was also resolved by the current study. Some investigators had theorized that ...Genetically engineered mosquitoes show resistance to dengue fever virus
...no effect on a single strand of RNA, but acts like scissors on the double strand, chopping it up and rendering its genetic material useless. Once this process is started, the single-stranded RNA also becomes vulnerable to dicer-2 and is cut up, thereby preventing further virus replication. On its own, this p...Expanding waistlines triggered by your genes
...not necessary, but it becomes a critical metabolic scissors once cells are inside 3-D environment either in collagen gel or in the real tissue of mouse. This finding may fill a gap between conventional 2-D cell biology and 3-D tissue function. These findings shed new light on adipocyte biology and possibly ...Living view in animals shows how cells decide to make proteins
...proteins the RNA produces within the cell. The scissors that do the genetic chopping are, in most cells, proteins called splicing silencers and splicing enhancers. In the current study, Garcia-Blanco’s team sought to identify which silencers chop out an important segment of RNA in a gene called fibrob...