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Tag: "recombination" at biology news

Mitochondrial genes move to the nucleus -- but it's not for the sex

...ear genome is recombination, after all, but little recombination takes place in self-pollinating species. So what, exactly, might be luring mitochondrial genes to the nucleus? "We're not quite sure why yet, but we've hypothesized that successful mitochondrial genes are pairing up with related nuclear genes," B...

Does evolution select for faster evolvers?

...A chain, or genome. A few theories have focused on recombination ?the process that occurs in sexual selection when the genetic sequences of parents are recombined. Horizontal gene transfer (HGT) is a cross-species form of genetic transfer. It occurs when the DNA from one species is introduced into another. The ...

New clues to how sex evolves

...en line up accurately and stay together during the recombination process. Different organisms use different methods for these critical steps; in C. elegans, the job is initiated by regions called Pairing Centers, which are found near one end of each of the worm's six chromosome. Dernburg's lab has been studying th...

Mapping the mouse genome

...heir maps, Shifman and colleagues then investigate recombination rates of various genes by comparing physical and genetic distances for each strain. On average, the recombination rate was 0.63 cM per megabase (cM/Mb) for HS, and 0.62 cM/Mb for RI. They also saw a lot of variatio...

Regulating the nuclear architecture of the cell

...nsed, repeated DNAs are then free to interact; DNA recombination and repair processes excise the DNA from the chromosome to form extrachromosomal DNA. If these extrachromosomal loops consist of ribosomal DNA, they further accelerate the production of misplaced nucleoli. Curiously, says Karpen, this chaotic sta...

Stanford discovery may help predict when toxoplasma can be deadly

...Boothroyd's group; the researchers found that the recombination of two relatively benign strains of Toxoplasma can result in a thousand-fold increase in their ability to cause serious disease. Over the last few years, the researchers have worked to track down exactly what happens to make some strains of Toxop...

What controls stickiness of 'smart' chromosomal glue

...aid. "For a long time, people did not think that recombination played any role in establishing the step-wise cohe...ttern," she said. "But our experiments showed that recombination is absolutely essential to remove cohesins from chromosome arms during the initial meiotic stages, a...

Proteins as parents

...that this exciting new application of the powerful recombination technique in the field of protein mechanics will open a new way to tailor proteins' mechanical properties. ...

Recombination protein dynamics observed with single monomer resolution

...e August issue of the journal Cell. RecA is a DNA recombination protein found in the gut bacterium E. coli. A huma... either end of the protein filament." During the recombination process, RecA binds with DNA to form a filament that spirals around the DNA. The filament can grow i...

Scientists force viruses to evolve as better delivery vehicles for gene therapy

...rase chain reaction (PCR) coupled with a test tube recombination technique. After reassembling the mutant viruses inside their capsids, they introduced them to blood serum pooled from rabbits immunized against AAV, and thus containing many types of antibodies to AAV. Only the mutant viruses good at evading antibod...

Housecat-sized Siberian tiger cubs get collared

...solated the SPANX region from 200 individuals by recombination in yeast." Based on their results, the authors ...l ?regions with higher rates of gene conversion or recombination ?that are indicative of genomic instability," explains Morrow. "With this knowledge in hand, we hop...

Studies on human genome variation provide insight into disease

...solated the SPANX region from 200 individuals by recombination in yeast." Based on their results, the authors ...l ?regions with higher rates of gene conversion or recombination ?that are indicative of genomic instability," explains Morrow. "With this knowledge in hand, we hop...

Can two killers make a cure?

...virus and the hybrid could somehow merge, but such recombination has never been observed in HIV. Despite his confidence that the hybrid will be safe, Pickles has abandoned similar research as he fears opposition from a fearful public will prevent the method ever being used. Another problem with Kobinger's approac...

Gene Bridges And Covalys To Develop Restriction-Enzyme-Free SNAP-tag Gene Fusion Kits

...-tag fusion proteins based on Gene Bridges' Red/ET recombination technology. Gene Bridge's Red/ET cloning system does not require restriction enzymes and allows the engineering of large DNA sequences, such as BACs, (bacterial artificial chromosomes) with single-nucleotide precision. SNAP-tag is an intelligent self...

New lifespan extension genes found

...howed that caloric restriction drastically reduces recombination of ribosomal DNA, and that deleting Hst2 and Sir2 blocks this effect. Very similar genes are found in widely different animals including worms, flies and rodents. But the targets of these genes are likely to be different, as the toxic DNA circles ha...

Brown-Harvard team solves mobile DNA's surgical sleight-of-hand

...ures? While scientists have studied site-specific recombination for about 30 years, much remains a mystery. In the current issue of Nature, a Brown University and Harvard Medical School team reveals the crystal structure of λ-integrase (λ-INT), the protein responsible for this DNA doctoring in the lambda virus, w...

Mosaic mouse technique offers a powerful new tool to study diseases and genetics

...omes contains a bad copy (mutation) of a gene, the recombination event could cause an offspring to inherit two bad copies of the gene, which would result in a mutant cell. This process simultaneously activates the green fluorescent protein, which turns the mutant cell green. "If there is no recombination, there a...

Researchers pioneer new gene therapy technique using natural repair process

... therapy only affects the mutant gene. Homologous recombination is a fairly rare event that occurs when DNA strand...echnique, researchers took advantage of homologous recombination by introducing a man-made enzyme that recognizes and binds DNA at specific points into human immune ...

To Stop Evolution: New Way Of Fighting Antibiotic Resistance Demonstrated By Scripps Scientists

...blem. If the damage persists, the cell upregulates recombination enzymes, which are tasked with recombining the DNA -- another way to repair it. And, says Romesberg, if the damage still persists, the cells upregulate enzymes whose sole task is to make mutations. Presumably, inducing mutations is an effective evol...

Building a human kinase gene repository

...ed verified, full-length, and can be sub-cloned by recombination based methodologies. This gene collection will be a useful resource for the scientific community and will facilitate high-throughput cellular and biochemical assays and structural studies of this important gene family. ...

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