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Tag: "recombinant" at biology news

Gene therapy delivery of nerve growth factors reverses erectile dysfunction in animal model

...plex virus (HSV). They then injected either of the recombinant viruses into the damaged cavernous nerve of rats. GDNF is an important nerve growth promoter and has been shown in other studies to contribute to survival and regeneration of penile nerves. Neurturin also is a nerve growth factor closely related to G...

UQ research heralds vaccine technology breakthrough

...oth diseases in the one shot and, even better, the recombinant vaccine model could potentially be used for most i...e in the research group is international expert on recombinant vaccines, Dr Scott Thomson. Professor Tindle said gaining Dr Thomson's expertise was a coup for the ...

Deconstructing brain wiring, one neuron at a time

...subsequent populations of cells are imaged. The recombinant rabies virus could contain genes for any proteins of interest, Callaway says, and he adds that once scientists can identify a neural circuit, they can then deactivate it, and test for changes in brain function. ...

Veterinary scientists explore poultry virus as cancer killer

... Reverse genetics is the process of generating a recombinant virus from cloned complimentary DNA (cDNA) copy of...ains Subbiah. Through the reverse genetics system, recombinant viruses can be designed to have specific properties that make them attractive as biotechnological to...

Scientists find hormone activity explains adolescent mood swings

... Smith and colleagues identified the site on human recombinant alpha4-beta2-delta GABA-A receptors that produced the anxiety response, and were able to mutate the site to prevent the novel effect of the stress hormone. In contrast, neither the receptor nor the necessary conditions exist for this anxiety-producin...

Vaccine-producing 'plant-factories'

...asts of the plant cell. Some of these glycosylated recombinant proteins have significant antigenic power of great... accumulate very limited quantities of glycosylate recombinant proteins. The chloroplast is a cell organ with great capacity for storing proteins. However, it is i...

Study uncovers mutation responsible for Noonan Syndrome

...on crystal structures of SOS1, the scientists made recombinant versions of the mutants and expressed them in mammalian cells, where it was discovered that they promoted excessive activation of RAS and its downstream target, MAP kinase, the same pathway activated by PTPN11 mutations. "These results are the fir...

'Failed' experiment yields a biocontrol agent that doesn't trigger antibiotic resistance

...bacterium. The host bacterium then replicates the recombinant plasmid and transfers copies of it to other bacteria in a process called conjugation. As he investigated the failed experiment, Filutowicz - who has spent two decades studying how plasmid replication is regulated - made a critical observation. A p...

Progress made in HIV vaccine development

...m three dominant HIV subtypes, and the second used recombinant adenovirus serotype 5 (rAd5) as a vector to delive...% experienced positive CD8 T cell responses. The recombinant vector vaccine was also well-tolerated, but higher doses led to some adverse events such as pain and...

GlycoFi and Dartmouth report full humanization of yeast glycosylation pathway in Science

...t cells capable of producing a broad repertoire of recombinant therapeutic proteins with fully human sugar struct...trate the utility of the engineered yeast strains, recombinant erythropoietin (Epo), a protein that stimulates the production of red blood cells, was expressed, pu...

Mapping the mouse genome

...outbred, heterogeneous stock (HS) and the other of recombinant inbred lines (RI). Using the single nucleotide polymorphism (SNP) data that resulted from physical mapping of the mouse genome, the authors were able to track the patterns of inheritance of >10,000 SNPs in both groups of mice. Next, they were abl...

Knockout Mouse Project

...ildren's Hospital Oakland Research Institute has a recombinant DNA library with more than 30 million DNA samples ...l research in the genetics of human diseases. The recombinant DNA library is, for example, being used in Oakland to investigate the genetic differences that make ...

Replacing insulin is top-ranked breakthrough foreseen for health in developing world

...Shantha Biotechnics, for instance, has developed a recombinant hepatitis B vaccine that sells for only $US O.40 per dose as compared to imported vaccines which sell for $US 8-10 per dose." Leaders among developing countries in their level of regenerative medicine activity are India, China and Brazil. The aut...

Stanford discovery may help predict when toxoplasma can be deadly

...s to eat. The researchers then took the resulting recombinant strains from the cats and infected other mice with them. Based on how these mice fared, the researchers could pinpoint stretches of DNA that contain the genes underlying severe virulence. Based on the DNA sequences, they could determine the protei...

Stem cell expansion

...ls. However, because HOXB4 protein is available in recombinant form (i.e. produced in cell culture, much like human insulin), it should be possible to treat HSCs directly with the protein, avoiding the potential problems associated with genetic manipulation of the cells. As the reviewers of the article commented...

Bacteria have their own immune system protecting against outside DNA

...otech industry, where bacteria are used to produce recombinant human proteins for medical treatments and research...ndustry, which uses bacteria for the production of recombinant proteins for medicine and research. These proteins, such as insulin or human growth hormone, are cre...

Innovative method for creating a human cytomegalovirus vaccine outlined

...y harmless to them. They then asked whether, using recombinant technologies, there might not be a way to shift th...trategy for developing a CMV vaccine, although the recombinant technologies available today should help to streamline the task. CMV infection is widespread in th...

A single sugar found responsible for an antibody's ability to treat inflammation

...ications, and his lab is now working to generate a recombinant form of IgG that, by virtue of a sialic acid molecule attached in the right place, will be anti-inflammatory and could act as a novel treatment for autoimmune disorders. "We have the opportunity to make a much better form of IVIG that will work 100 t...

Plant-derived vaccines safeguard against deadly plague

...en during the same challenge. "The idea with any recombinant subunit vaccine is that you can pick and choose selective antigens that can confer protection and limit the potential for adverse reaction," said Mason. More than half the vaccinated animals survived the challenge with all forms of the antigen, and ...

Do plants have the potential to vaccinate against HIV?

...t a generic strategy to increase the expression of recombinant proteins in plants. It could open the door to cheaper biopharmaceuticals. Plant-derived pharmaceuticals are of great interest because of their enormous potential for economy and scale of production. This technology could lead to production of modern ...

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