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Tag: "microarrays" at biology news

CSIRO scientists join fight to save 'Tassie devil'

... use a variety of techniques including microscopy, microarrays and a range of molecular techniques to search for infectious agents, markers for disease and to determine where the tumours originate. “We will be working in a number of areas including establishing whether a virus or other infectious agents ar...

Study identifies 5 genetic themes key to keeping stem cells in a primitive, flexible state

..., the database includes data from thousands of DNA microarrays submitted mainly by scientists in Canada’s Stem Cell Network. All data is freely available at www.stembase.ca . Lead author Ms. Pearl Campbell noted that understanding how stem cells maintain their identity is key to the emerging field of regene...

Liver regeneration may be simpler than previously thought

...r liver were removed. Using techniques such as DNA microarrays ?which determine which genes are active in a cells ?and software programs that analyze the collected information, the scientists listed all the proteins that help the cells grow and proliferate in both samples. The results were unexpected. The r...

Scientists map key landmarks in human genome

...eosomes are located." The researchers used gene microarrays to which DNA associated with single nucleosomes was added. The nucleosomal DNA was derived from several cancer cell lines including melanoma and breast cancer, as well as several normal human cell types. A critical component of the analysis involved ...

Mutant gene causes severe kidney disease in infants

...p until later in life." U-M scientists used DNA microarrays to analyze blood samples from 26 families around the world who had children diagnosed with steroid-resistant nephrotic syndrome. After eliminating known mutations, the study team was left with DNA from 12 children with the disease. All 12 had inherit...

Researchers identify a key regulator for skin stem cells

... gene on at will in skin cells. They then used DNA microarrays to analyze which genes were affected when Tcf3 was activated. Microarrays, also known as "gene chips," enable scientists to determine the activity of thousands of genes at once. "When we compared the list of genes that Tcf3 repressed or induced, ...

Selecting life: Scientists find new way to search for origin of life

...rwhelmingly large number of possibilities." DNA microarrays provide a means to address this problem. Microarrays are produced robotically to spot tens of thousands of microscopic droplets of DNA from as many genes onto a slide, enabling scientists to measure which genes are turned on. This rapidly developing ...

Unraveling where chimp and human brains diverge

...nd biostatistics, the UCLA team used data from DNA microarrays ?vast collections of tiny DNA spots -- to map the activity of virtually every gene in the genome simultaneously. By comparing gene activity in different areas of the brain, the team identified gene networks that correlated to specific brain regions....

First biomarker for human sleepiness identified in fruit flies

...s to different kinds of sleep deprivation and used microarrays to look for changes in activity in many different genes. Amylase levels consistently changed after sleep loss. Amylases are a family of enzymes found in the saliva that break down starch. To verify amylase's connection to sleep loss, Shaw's lab mo...

Mouse study reveals new clues about virulence of 1918 influenza virus

...d Kash and colleagues examined the mouse RNA using microarrays to determine which genes were activated when exposed to each of the four viruses. This analysis showed that the immune response to the reconstructed 1918 virus containing all eight flu genes was much greater than to any of the other viruses with all ...

Einstein researchers take the pulse of a gene in living cells

...ong part of the body. Researchers customarily use microarrays (also known as "gene chips") to assess gene expre...ther tissues. But with millions of cells involved, microarrays reflect only "average" gene expression. Just how a gene is transcribed in a single cell--continuousl...

Speeding the search for elusive chromosomal errors

...retches of DNA within a sample being tested. These microarrays hold more than 100,000 DNA oligonucleotides, which...ntire complement of DNA in a cell nucleus. "These microarrays provide more rapid and precise results than karyotyping, and offer as much as 50 times higher resolu...

Predicting chemotherapy outcome

...nd Marie Curie University, Villejuif, France, used microarrays to analyse the gene-expression patterns of samples from colon tumours and liver metastases collected from 13 patients with colorectal cancer. The microarray analyses were carried out before the patients were treated with combined chemotherapy of foli...

Absence of critical protein linked to infertility

...e than four years ago. First, researchers used DNA microarrays to identify gene expression under normal and abnormal conditions during implantation. After messenger RNA profiling zeroed in on C/EBPb's activity, the researchers collaborated with Peter F. Johnson of the National Cancer Institute's Laboratory of Pr...

Yale scientists decipher 'wiring pattern' of cell signaling networks

...ts Jason Ptacek and Geeta Devgan used proteome microarrays to assay the thousands of different proteins in a ...in the yeast cell, were tested separately with the microarrays to determine which proteins were modified by each kinase. From the wealth of information generated ...

Carnegie Mellon U. transforms DNA microarrays with standard Internet communications tool

...ivation in living, complex biological systems. DNA microarrays monitor the behavior of thousands of genes over ti...and cost-effective," Bar-Joseph added. "While DNA microarrays are very powerful, they present a sampling problem," Bar-Joseph said. "DNA microarrays only take sta...

Adult stem cells aid recovery in animal model of cerebral palsy

...erpinnings of speciation, the researchers used DNA microarrays to identify global differences in the mosquito gen...in terms of methodology, this study shows that DNA microarrays can be used to identify regions of the genome that are different between two diverging species, allo...

Islands in the Genome Promote Speciation

...erpinnings of speciation, the researchers used DNA microarrays to identify global differences in the mosquito gen...in terms of methodology, this study shows that DNA microarrays can be used to identify regions of the genome that are different between two diverging species, allo...

How Fruitflies Know It's Time for Lunch

...of other neural proteins, the researchers used DNA microarrays to compare gene expression in normal flies to that in klu mutants. Any klu-controlled genes expressed at different levels in klu mutants might contain clues about the neural circuitry modulating feeding behavior. Using microarrays, Melcher and Pankr...

Nanoparticles, nanoshells, nanotubes: How tiny specks may provide powerful tools against cancer

...tion methods to make thousands of sensors and have microarrays of these to detect cancer proteins." The researchers plan to test the technique with additional breast cancer markers, as well as with markers for other cancers. They are also planning to perform animal studies, examining the sensitivity of the ant...

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