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Tag: "inserts" at biology news

Experimental gene therapy 'abolishes' arthritis pain and lessens joint damage

...ainkiller, researchers found. Gene therapy inserts tailor-made genes into cells that can, for instance, direct cells to build more of a needed protein. To deliver the genes into cells, researchers use harmless viruses called vectors, which have evolved to invade human cells and insert their DNA. In d...

Magnetic system could be key to surgery without scars

...the abdomen for natural orifice surgery, which now inserts the instruments through the throat, colon or vagina. “The current state of the art for laparoscopic surgery requires four or five holes. The question behind this is, can we do the surgery through only one hole and can we hide the hole in a cosmeti...

Genome archaeology illuminates the genetic engineering debate

...ate an active evolutionary process in which nature inserts genes much like modern biotechnologists do. Now we must reassess the allegations that biotechnologists perform 'unnatural acts,' thereby creating 'Frankenfoods,'" said Professor Joachim Messing, project leader and director of the Waksman Institute of...

Mayo researchers discover HIV dependence on a human protein

...he human genome. Their findings show that when HIV inserts itself into a chromosome, a key step that enables it to establish a "safe haven," it requires a specific protein -- LEDGF/p75 (p75). This protein forms a molecular tether between chromosomes and HIV's integrating protein (integrase). If the connectio...

P(acman) takes a bite out of deciphering Drosophila DNA

...nd then try to put that gene back into the fly, it inserts itself randomly into the genetic blueprint. In some cases, it makes too much protein, and in others, too little. In other instances, it may disrupt the message from another gene. “You are really comparing apples and oranges when you do this,?...

Interfering RNA silences genes in 'slippery' immune cells

...desired SEC into a vector, a biological agent that inserts itself into a target cell's nucleus more efficiently than an unaccompanied cassette. The researchers first tested their approach by introducing a gene for green fluorescent protein into human T cells, and using siRNA to inhibit that gene's expressi...

Waking a sleeping virus

...ent to its active state. "During an infection, EBV inserts its own DNA into the nucleus of human cells. This information contains the codes for about 100 genes," says Christoph Müller, head of an EMBL research lab in Grenoble. "Less than 10 genes are sufficient for the virus during its latent state, whereas ...

SLU researchers uncover direct evidence on how HIV invades healthy cells

...into deoxyribonucleic acid (DNA). Integrase then inserts the HIV DNA into the immune cell's DNA, making it a permanent part of the cell. The third protein, protease, processes viral proteins and is essential to make infectious virus. Drugs such as AZT and drug combinations (cocktails) exist to inhibit r...

New plant DNA libraries provides massive boost to world's plant researchers

...eate plant DNA libraries with large and "unbiased" inserts that give the best possible representation of the DNA of each species. Genomic DNA libraries usually employ "restriction enzymes" to cut up the DNA but this method may preferentially select only certain regions of DNA and exclude other regions from t...

New technique could alter field of mouse genetics

...s working with vertebrates and mammals." PiggyBac inserts randomly into the genome, with a clear preference for genes. This bodes well for its use in mutating genes and for identifying unrecognized genes in places of the genome that have been especially difficult to sequence. Furthermore, the markers it car...

Brown-Harvard team solves mobile DNA's surgical sleight-of-hand

...k represents a major leap." When the lambda virus inserts its DNA into E. coli, λ-INT is the major player in the process, literally snipping the E. coli DNA strands before insertion and then sealing them up afterward. The research also suggests how three "helper" proteins ?think of them as nurses ?intricate...

Viral protein influences key cell-signaling pathway

...through breast milk. HTLV-1, like its cousin HIV, inserts its genetic information permanently into the DNA of a T lymphocyte and remains there for the life of the cell. HTLV-1 infection is also lifelong. A hallmark of HTLV-1 infection is the proliferation of T lymphocytes. This sets HTLV-1 apart from HIV, ...

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