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Tag: "fluorescent" at biology news

Purdue's gold nanorods brighten future for medical imaging

... images nearly 60 times brighter than conventional fluorescent dyes, including rhodamine, commonly used for a wid...her contrast and brighter images than conventional fluorescent imaging methods. Photons are the individual particles that make up light. In two-photon fluorescenc...

Method slashes quantum dot costs by 80 percent

...tant advance toward the large-scale manufacture of fluorescent quantum dots, scientists at Rice University have developed a new method of replacing the pricey solvents used in quantum dot synthesis with cheaper oils that are commonplace at industrial chemical plants. Rice's study, which was conducted under the ...

Research casts doubt on circulating stem cells

...ed the bone marrow with healthy marrow marked by a fluorescent gene," explains Bonn physiologist Professor Dr. Anton Wernig. The hope of the medical scientists is that the transplanted stem cells will find their way through the blood into damaged muscle fibres where they can produce functioning dystrophin. The B...

Prions rapidly 'remodel' good protein into bad, Brown study shows

...rchers tagged a non-prion form of Sup35 with green fluorescent protein in one group of cells and "mated" these cells with another group that contained the prion form. When the two forms came in contact in the same cell, the green-glowing, healthy protein changed pattern - a visual sign that it converted to the p...

A step toward the $1,000 personal genome using readily available lab equipment

...e resolved through inexpensive optics. One of four fluorescent dyes corresponding to the four DNA bases binds at a specific location on the genetic sequence, depending on which DNA base is present. The fragment then shines with one of the four colors, revealing the identity of the base. Recording the color data ...

NHGRI expands effort to revolutionize sequencing technologies

...The key to the system is the interaction between a fluorescent polymerase and the nucleotide, which emits a signature detectable in real-time. Xiaohua Huang, Ph.D., University of California, San Diego, La Jolla. $750,000 (3 years) "Massively Parallel Cloning and Sequencing of DNA" The goal of this project is t...

New law for resolution allows unprecedented sharpness in fluorescence microscopy

... structures (which had been previously dyed with a fluorescent dye) of width as small as 40-80 nm, a size previously reserved for electron microscopy. This may be of importance for the lithographic manufacturing of microchips. The reduction in size of the diameter of the effective fluorescence focus also has fu...

New technique could alter field of mouse genetics

...to find. In Xu's lab, the jumping gene carried red fluorescent protein and an enzyme that changes the coat color of a white mouse to grey or black. The transposon acts as a genetic beacon, so researchers can easily track its location without having to sequence the entire genome, as can happen with the chemical m...

Nanoscale method for investigating living systems

...ncoded with different colors making them useful as fluorescent labels for staining cells. Their resistance to photobleaching and physical size of less than 10 nanometers are making them increasingly popular in biomedical applications ranging from intracellular tagging of molecules to applications such as trackin...

'Underground' tunnels discovered as means for communication between immune system cells

...f stimulation in numerous cell types. The use of a fluorescent dye, which allows direct measurement of calcium levels, would determine if calcium flux occurs before dendritic cells unfurl their veils. With a microinjection tip, they squirted a mixture of E. coli fragments into a culture dish, and, indeed, one t...

Unweaving amyloid fibers to solve prion puzzles

...me into contact with each other, they would emit a fluorescent signal, allowing him to map the pattern by which the different strains of prions interacted with each other. He found that each prion molecule had only two points at which they connected to other prion molecules. One point he called the "head," the ...

UCSD study clarifies insulin's role in blocking release of energy in patients with type II diabetes

...eporter is a “marker?protein, created with special fluorescent tags so that scientists can physically view the protein under a microscope and watch how the live cell activates PKA in real time. The PKA is normally activated inside the adipocyte cell, the major site of energy storage in the body where many aspect...

Several minute intermediate stage in virus-cell fusion discovered; opportunity for drug development

... only 100 nanometers across, by labeling them with fluorescent dyes and recording at one frame every 7 seconds. (For comparison purposes, a human hair is 80,000 nanometers thick.) ASLV is a useful model for several viruses, including Ebola, the 'flu, and measles, as well as HIV. "This system is especially well ...

Quantum dots provide a faster, more sensitive method for detecting respiratory viral infections

..., high-tech method uses multi-colored, microscopic fluorescent beads, called quantum dots, which bind to molecula...in a tissue culture for five days and then using a fluorescent dye to test for the presence of the virus. The main problem with this technique is that the virus is...

A novel method to measure circadian cycles

...cultures with a virus engineered to report with a fluorescent signal when a certain host circadian rhythm gene was expressed. They found that their data jibed with the previously accepted length of the human circadian cycle: 24.5 hours. Because of the sensitivity of their method, Schibler and colleagues also co...

Tagging pathogens with synthetic DNA 'barcodes'

...n be read by a computer scanner or observed with a fluorescent light microscope. Other methods of identifying bi...f the DNA. To other ends are attached molecules of fluorescent dye in a predetermined pattern. For example, one probe might contain four molecules of green dye an...

Chemical 'band-aid' prevents heart failure in mice with muscular dystrophy

... myocyte membranes, U-M researchers used a special fluorescent dye, which cannot penetrate an intact cell membrane. After a 20 percent stretch, control myocytes remained stable and showed no fluorescence, while myocytes from mdx mice became unstable, started shaking and showed a steady increase in fluorescence, ...

Bitter or sweet? The same taste bud can tell the difference

...ot both peptides were present. This procedure uses fluorescent light to let researchers actually see the peptides under a microscope. They initially found that NPY is expressed in only a subset of taste bud cells. Yet every cell that expressed NPY also expressed CCK. "That surprised us, too," Herness said. "It...

Scientists devise way to measure RNA synthesis on the fly in a live cell

...led MS2. This MS2 protein also is fused to a green fluorescent protein that a laser can detect within a microscop...lume. In the absence of RNA, the MS2 fused to the fluorescent protein moves rapidly. But when bound to RNA, the fused proteins move more slowly. From the speed of...

Gene Bridges And Covalys To Develop Restriction-Enzyme-Free SNAP-tag Gene Fusion Kits

...ag fusion proteins in live cells and in vitro with fluorescent or affinity probes. Kits to be introduced later this year by Covalys will allow for immobilization without prior purification, and purification of SNAP-tag fusion proteins. "We are particularly happy about this collaboration," said Christoph Bieri, ...

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