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Tag: "fluorescent" at biology news

APS lecturer shows rare video of 'teacher-student' immune cell interactions in live animal

...i-photon microscopes using infrared beams to image fluorescent cells in living tissues became commercially availa...Institute and developed a strain of mouse that has fluorescent T-cells. "We are among a handful of labs which started to do this at the same time," von Andrian sa...

New Research Shows Artificial Light at Night Stimulates Breast Cancer Growth in Laboratory Mice

...ghttime following 90 minutes of exposure to bright fluorescent light. These blood samples were then pumped directly through the developing tumors. “The melatonin-rich blood collected from subjects while in total darkness severely slowed the growth of the tumors. “These results are due to a direct effect of the ...

Report lists top 20 most-vulnerable African carnivores

...he help of bacteria and a protein that emits green fluorescent light, Jacobs randomly screened tens of thousands ...ntage of the fact that when multiple copies of the fluorescent protein clump together, the fluorescence is quenched, or extinguished. So Jacobs engineered bacteria...

Groovy protein essential for promoting cancer development

...he help of bacteria and a protein that emits green fluorescent light, Jacobs randomly screened tens of thousands ...ntage of the fact that when multiple copies of the fluorescent protein clump together, the fluorescence is quenched, or extinguished. So Jacobs engineered bacteria...

'Shuttling' protein possibly key to resilience of cancer cells

...esearchers tracked ATF2 and other proteins using a fluorescent imaging technique developed by Hu called bimolecul...complementation. The procedure involves breaking a fluorescent protein into two fragments and then fusing each fragment to different AP-1 proteins, including c-Jun...

Blood flow in brain takes a twist, affecting views of Alzheimer's

...of astrocytes in living organisms. The team used a fluorescent dye to light up the blood vessels, then put a special form of the chemical calcium into astrocytes. They used one laser to activate the calcium, and another laser to monitor how astrocytes processed the chemical. They found that astrocytes caused blo...

Mice with glowing hearts shed light on how hearts develop

...cience Institute in Wako-shi, Japan, developed the fluorescent molecule by modifying a green fluorescent protein (derived from bioluminescent jellyfish) and making it glow brightly enough to be observed in...

Biologists develop genome-wide map of miRNA-mRNA interactions

...stem comparing the expression of a reporter, green fluorescent protein (GFP) carrying target 3' UTRs with controls, that did not carry the target 3'UTRs. The laboratory results confirmed the role of specific 3' UTRs in suppressing gene expression even more widely than predicted by the computational analysis, sug...

Strawberries by design

... using A. tumefaciens. Since they glow green under fluorescent light due to the presence of Green Fluorescent Protein (GFP), transformed strawberry plants may be easily identified by visual inspection. This is the first time that GFP has been used in strawberry as a visually selectable marker. Commenting on t...

High-resolution light microscope reveals the fundamental mechanisms of nerve communication

...e function remained unanswered. In contrast, using fluorescent molecules as markers, one can specifically label i...ency, rendering them visible with the conventional fluorescent microscope. Unfortunately the high resolution required to separate nanoscale structures was lacking ...

New brain hormone puts brakes on reproduction

...ch in his own lab as an assistant professor. Using fluorescent antibodies to GnIH, they were able to locate where in the brain the hormone is made: in the nerves of the dorsomedial hypothalamus. The axons of these nerves project to numerous areas of the brain where nerve cells produce GnRH, and staining showed t...

Molecule by molecule, new assay shows real-time gene activity

...limitations. He and his colleagues melded a yellow fluorescent protein called Venus with Tsr, a hydrophobic membr...ins zipping about in cell cytoplasm, where diffuse fluorescent signals tend to be overwhelmed by background noise. The gene coding for this combined protein was s...

A new focus for the mechanism of nerve growth

...lopmental biology at Yale used a technique called fluorescent speckle microscopy, or FSM, that let them directly see actin filament assembly, disassembly and movement in living cells. They used FSM to monitor actin dynamics in nerve cells treated with a new drug called blebbistatin, that relaxes non-muscle myos...

Sea coral's trick helps scientists tag proteins

... protein that generates the light to create a tiny fluorescent tag that responds to visible light. The two-color ...al processes," he said. Unlike other green-to-red fluorescent markers, Dendra can be activated by visible blue light, which is less harmful to living cells and do...

Formation of cellulose fibers tracked for the first time

...udent Alex Paredez, engineered plants to produce a fluorescent version of cellulose synthase, the enzyme that creates cellulose fibers. They also included a fluorescent version of tubulin, the protein from which microtubules are built. Using an imaging technique that c...

Researchers solve mystery of how nuclear pores duplicate before cell division

...ells of a single pore that had been labeled with a fluorescent tag. If the nuclear pore had split to give rise to two daughter pores, two bright dots would have emerged from one; however, the researchers tracked movement of only one dot, confirming their previous finding that pores formed from scratch. Addition...

How taste response is hard-wired into the brain

...of the flies, the researchers generated flies with fluorescent labels on their neurons that would signal activation of one or the other type. They used microscopic imaging through tiny windows in the fly brains to watch neuronal response when they exposed the flies to sweet or bitter chemicals They found that a...

Malaria parasites develop in lymph nodes

...om each mosquito bite, they found an average of 20 fluorescent parasites embedded in the animal's skin. Ménard found that the parasites moved through the skin in a random, circuitous path at a speed that is amongst the fastest recorded for any migrating cell. After leaving the skin, the parasites frequently inva...

Flesh-eating bacteria escape body's safety net

...ading ulcer in the mouse infection model. Special fluorescent microscopy techniques were used to observe how the Strep enzyme dissolved the DNA nets and allowed bacteria to float away from the neutrophils. "The experiments explain how this DNA-degrading enzyme contributes to the severe infections produced by c...

Patterns in genome organization may partially explain how microbial cells work

...ar way inside the cell. New techniques that attach fluorescent "reporter" markers to predetermined spots on chromosomes have indicated that many bacterial genes tend to be found at specific cellular locations. Patterns are not obvious in the sequences of prokaryotic genomes, which led the team led by Palsson to ...

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