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Tag: "fluorescent" at biology news

OHSU study says stem cell 'fusion' occurs in tumors

... within the female donor cells marked with a green fluorescent protein. About 60 percent of the epithelial cells ...ntestinal cells for the presence of both the green fluorescent protein, indicating female donor cells, and an enzyme from the male recipient cells Wong said, "Our...

Healing the heart with bone marrow cells

...h bone marrow cells were modified to carry a green fluorescent marker allowing researchers to easily track them. The researchers demonstrated that these bone marrow cells are quickly mobilized to the damaged heart region following a heart attack. Once in the damaged area, the cells produce chemicals that trigger...

Biological motors sort molecules one by one on a chip

...the researchers allowed a mixture of green and red fluorescent microtubules to arrive at a Y-junction. By changing the direction of the electrical force, depending on the color of the microtubule, the Delft researchers were able to collect the green and red microtubules in different reservoirs. With their app...

Einstein researchers take the pulse of a gene in living cells

...ar intervals. Dr. Singer and his co-workers used a fluorescent marker that sticks to the gene only when it is active. Under a microscope, this fluorescent marker appears when the gene turns on, then disappears (gene "off") and then appears again (gene "on...

Movie spies on malaria parasite's sneaky behavior

...' journey. By using parasites labeled with a green fluorescent marker and staining the mouse's blood vessels with a red fluorescent marker, Amino was able to record microscopic images inside the animal's liver. He found that not onl...

Scientists solve sour taste proteins

... the brain. In their study, the researchers used fluorescent tags to label the subsets of cells that are known ...ntists loaded the cells with two calcium-sensitive fluorescent dyes -- one that glowed green when the calcium concentration was high, the other that glowed red whe...

Surprise finding for stretched DNA

...ion to stretch the DNA molecule. With the use of a fluorescent coating, the subsequent spinning of the rotor bead in response to the stretching can be recorded. "When we apply tension to the DNA molecule, changes in the rotor bead angle reflect changes in the twist of the lower DNA segment," Bustamante said. "...

Pathway toward gene silencing described in plants

... to watch the pathway function in live cells using fluorescent proteins and time-lapsed microscopy, to learn even more." ...

Researchers offer clues to how leaves patterns are formed

...e protein that transports the hormone auxin with a fluorescent tag, he could then shine a light on the leaves and watch how auxin was being transported during vein formation. Thanks to this approach, the team identified cells within individual veins that transport the hormone auxin in two opposite directions. He...

New roles for growth factors: Enticing nerve cells to muscles

...le. Finally, using mice engineered to express a fluorescent protein in MMCm neurons, the investigators demonstrated that only the glowing neurons extended axons in the direction of target cells expressing FGF. "After a lot of hard work, we narrowed it down to FGFs and showed that they were indeed the long so...

First-ever genomic test predicts which lung cancer patients need chemotherapy to live

...he cell. Scientists label the messenger RNA with fluorescent tags. The fluorescent RNA is then placed on a tiny glass slide, called a gene chip. There, it binds to its complementary ...

Encoded metallic nanowires reveal bioweapons

...oint, free antibodies that have been tagged with a fluorescent dye are added to the sample. These also dock onto the pathogen so that it is surrounded like the filling in a sandwich; giving the technique its name, "sandwich immunoassay". Measurement of the fluorescence now gives information about the pathogen co...

New light microscope sharpens scientists' focus

... a paper in Science showing that the position of fluorescent molecules under a near-field optical microscope c... the world of biology there is a new generation of fluorescent proteins that you can switch on at will with a little bit of violet light," Hess explained. He and B...

Gold nanoparticles could improve antisense cancer drugs

...rgeted mRNA sequences that code for enhanced green fluorescent protein (EGFP) expressed in a mouse cell. The antisense sequence of the DNA attached to the nanoparticles was complementary to the mRNA for EGFP expression. When the nanoparticles were introduced to the cells the fluorescence dimmed -- a result of th...

Life and death in the hippocampus: what young neurons need to survive

... born neurons infected with virus were marked by a fluorescent dye enabling detection of neurons derived from tho...ater, animals that received the virus showed fewer fluorescent neurons compared to mice injected with a benign virus lacking the shears, meaning fewer new neurons ...

UT Southwestern researchers find gene mutation that leads to 'broken hearts'

... hearts glow by expressing a gene coding for green fluorescent protein specifically in the heart. "This fly with a glowing heart enables us to visualize the details of heart development with high resolution in living animals and to detect cardiac defects that have never been described before", said Dr. Zhe Han, ...

Long-term changes in experience cause neurons to sprout new long-lasting connections

...e that were genetically altered to produce a green fluorescent protein in specific neurons in the neocortex, which is a region of the brain that is known to adapt to new experiences. The researchers followed the growth of dendritic spines in the region of the neocortex that processes tactile information from the...

New genes implicated in rheumatoid arthritis

...rom the cells of every twin, labelled samples with fluorescent dye to distinguish RA cells from disease-free cells, and hybridized each on a 20,000-gene chip. Then, using immunohistochemistry and real-time polymerase chain reaction, they confirmed the expression of the most significantly over-expressed genes in...

New human retrovirus originated in mice

...each sample and tagged the genetic material with a fluorescent tracer. They then applied the fluorescently tagged genetic material to the microarray chip. Since genes tended to adhere to those with a complementary genetic sequence, any viral gene sequences in the sample would attach themselves to corresponding v...

USC researchers investigate protein that protects tumors

...the study's senior author. The scientists used a fluorescent dye attached to the protein's antibody to reveal the protein's location on the tumor cells. "The first step was to identify whether it's there [on cancer cells] and how often," he explained. "We found that it was present on 60 percent of the tumor...

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