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Tag: "fluorescent" at biology news

Brown scientists map structure of DNA-doctoring protein complex

...protein complexes in a gel. He tagged the DNA with fluorescent dyes and purified the proteins, placing them in a gel that was then shot through with light. Sun measured the wavelengths of light as they bounced between the molecules of dye. Those measurements were then fed into a special software program created ...

Lab-on-a-chip could speed up treatment of drug-resistant pneumonia

...to certain strains of bacteria, and mark them with fluorescent dyes of different colors. The dyes color-code cells from known strains. A microscope monitors the viable cells -- those that are still reproducing -- and the rate at which they duplicate helps to identify their species. In the next step, different...

Embryonic patterning makes the feathers fly

...ve cell movements and track individual cells using fluorescent markers. The results were recorded in real time by time-lapse video microscopy. The resulting chicken feather movie resolved the question: the FGF/MAPK pathway acted through the mesenchyme. Analyses of fluorescent-labeled cells confirmed an incre...

Columbia University licenses next-generation DNA sequencing technology

...ted with being one of the primary inventors of the fluorescent energy transfer chemistry for 4-color Sanger sequencing being used by virtually all of the current generations of DNA sequencers that were used to complete the Human Genome Project. ...

The biggest bug in gut discomfort

...ed cells. Taken up into the endocytic pathway, the fluorescent dyes co-localized with control bacteria known to traffic to lysosomes, yet surprisingly the dyes were unable to enter vacuoles containing C. jejuni. The bug had left the conventional endocytic pathway. Watson and Galan also investigated the roles...

A fisheye view of the deadliest breast cancer

... metastatic forms of breast cancer. Using various fluorescent tags, they labeled human MDA breast cancer cells i...by inducing an angiogenic response. A separate fluorescent labeling allowed the researchers to simultaneously monitor the parental MDA cancer cells and the Rho...

Nanotech tools yield DNA transcription breakthrough

...ce spectroscopy. The researchers attached pairs of fluorescent "tags" to key structural elements of the machine and then monitored changes in distance between tags in single molecules as transcription occurred. The researchers showed that, during initial transcription, the machine does not move to reach adjacent...

UCLA researchers unravel a mystery about DNA

...n -- and hence the distance separating -- pairs of fluorescent chemical tags attached to key structural elements of RNAP and the DNA double helix during initiation of the transcription process. The changes in the distances between these tags confirmed that transcription proceeds initially through a "scrunchi...

Newly discovered behavior in cancer cells signals dangerous metastasis

...ncer cell would turn, the scientists constructed a fluorescent "reporter" -- a protein that illuminates if the cell turns epithelial but lies dormant if the cell reverts to mesenchymal state. By following the reporter's illumination within cancer cells in rats, the team viewed the very process of alternative sp...

Biofuel cells without the bio cells

... a combination of techniques that included FCS, or fluorescent correlation spectroscopy, and confocal microscopy. These yielded a "fluorescence intensity trace" whose brightness depended entirely on whether hematite was available to bind with OmcA in solution. No hematite, dim; hematite, bright. How bright? ...

Stem cell activity deciphered in the aging brain

...heir census, the researchers attached easy-to-spot fluorescent tags to the neuronal stem cells in the hippocampus...ting material. The researchers then used another fluorescent molecule to tag all stem cells that were undergoing division in the process of staying "fresh" in ca...

Getting to the heart of the heart

...these cardiac progenitors was indicated by a green fluorescent protein, which lit up when a gene called Nkx2.5 wa...s and smooth-muscle cells. Next, using the same fluorescent "tags," Orkin and Wu isolated the same cardiac progenitor cells directly from live mice early in emb...

Researchers use multiphoton microscopy to watch chromosomes in action

...nd by using fruit flies specifically bred to carry fluorescent proteins on HSF, they could watch the transcription factors in action. "This is the first time ever that anyone has been able to see in detail, at native genes in vivo , how a transcription factor is turned on, and how it then is activated," said ...

UGA scientists unravel 'molecular inch-worm' structure of walking-pneumonia bacterium

...am went further, using fluorescence microscopy and fluorescent protein fusions that allowed them to track the act...his research demonstrates the feasibility of using fluorescent proteins to study how organelles in these incredibly tiny bacteria grow and what their functions are...

Pure carbon nanotubes pass first in vivo test

...D. Anderson. "We are particularly pleased that the fluorescent effect remains intact in our application, because this makes it easier to see where the nanotubes end up, and it opens the door to some exciting diagnostic and therapeutic applications." In a ground-breaking 2002 study, Weisman and colleagues at R...

New mechanism explains glucose effect on wakefulness

...ents, the researchers engineered mice to produce a fluorescent protein only in orexin neurons. Thus, the researchers could isolate the neurons in brain slices from the mice and perform precise biochemical and electrophysiological studies to explore how glucose acted on those neurons. In particular, the researche...

Researchers find protein that silences genes

...en genetically engineered the protein to include a fluorescent tag and found that much of the HDA6, seen as a glowing red signal under the microscope, shows up in the nucleolus, which is precisely the site where ribosomal RNA genes are regulated and where nucleolar dominance occurs. "We found HDA6 at the scene ...

Study provides first look at the 'birth' of a retina cell

... DNA sequence to produce a protein that appears as fluorescent green when exposed to blue light. This fluorescent protein allows scientists to identify even a few cells that are destined to be rods at very early st...

Interfering RNA silences genes in 'slippery' immune cells

...ted their approach by introducing a gene for green fluorescent protein into human T cells, and using siRNA to inhibit that gene's expression, and dim its fluorescent glow. They then applied their approach to HALP, a gene naturally active in T cells. Dr. Finkel pre...

A surprise about our body clock

...h bone marrow cells were modified to carry a green fluorescent marker allowing researchers to easily track them. The researchers demonstrated that these bone marrow cells are quickly mobilized to the damaged heart region following a heart attack. Once in the damaged area, the cells produce chemicals that trigger...

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