Scientists use nanoparticle to discover disease-causing proteins
...ndrimers would carry one of the stable isotopic or fluorescent labels to identify the presence or absence of a protein that can be further developed for use as a disease indicator, or biomarker. Snake venom cells were used because they have a very high concentration of proteins similar to some found in human ...Scripps research study reveals structural dynamics of single prion molecules
...ution technique that measures time fluctuations in fluorescent emissions from tagged proteins, which provided information about changes in shape of Sup35 taking place on the nanosecond timescale (billionths of seconds). A third technology, single molecule fluorescence coincidence, was used in an unusual way-...Scientists discover new life forms in the Arctic Ocean
...er, bili because they contain biliproteins, highly fluorescent substances that transform light into biomass, and phyte meaning they are plants. The discovery came from carefully analyzing DNA sequences belonging to vast communities of micro-organisms living in the ocean. "There was one group of sequences that...Biologists prove critical step in membrane fusion
...are shared but the contents remain separate. Using fluorescent dyes and a high-resolution microscope, the researchers show that hemifusion is surprisingly stable in live cells. "The novelty of these results is that a live cell can maintain a hemifused state for hours, days, even months," said Julian Wong, a p...Scientists create dual-modality microbeads to improve identification of disease biomarkers
...emistry. The technology involves embedding fluorescent quantum dots and magnetic iron oxide nanoparticles inside the beads to create dual-modality magneto-optical beads. Nie and Sathe synthesize the quantum dots in different colors by varying their size, giving the beads a unique optical signature. Havin...Scientists unlock secret of what makes plants flower
...otein through the plant by tagging it with a green fluorescent protein originally isolated from jellyfish, allowi...ther, only one of which contained the gene for the fluorescent version of FT. This allowed them to show conclusively that FT protein moved from where it was produc...The connector rotation hypothesis? Phage do not pack their DNA by rotation
...ntly, they also labeled the connector complex with fluorescent dye molecules so they could observe its motion usi... packaging in six รถ29 mutants. After attaching the fluorescent molecules to the connector complex, they set the mutants to work packaging DNA in a flow chamber. As...Ultrathin films deliver DNA as possible gene therapy tool
... stents can successfully deliver a gene encoding a fluorescent protein into a rabbit's artery, demonstrating that the films can also work in the complex environment of living tissue. Lynn presented a summary of the work at the annual spring meeting of the American Chemical Society in Chicago on Monday, March 26...Immunization rates hit record high in poor countries
Reaching into a box glowing with fluorescent light, Stacie Grassano pulls out a tube. "This is a great one," she says, holding the clear plastic up to her face. Inside, a tree branch is speckled with white fluff. "It's growing really well," she says, handing it to Scott Costa. Costa brings the...Fungal factories may save hemlock forests
Reaching into a box glowing with fluorescent light, Stacie Grassano pulls out a tube. "This is a great one," she says, holding the clear plastic up to her face. Inside, a tree branch is speckled with white fluff. "It's growing really well," she says, handing it to Scott Costa. Costa brings the...Gene key to taste bud development identified
...o produce a special protein, called enhanced green fluorescent protein, that glows green when exposed to ultraviolet light. "When we shine light on tissue from these animals, any cell that is expressing SOX2 will fluoresce, or light up," Pevny explained. "This allows us to directly visualize those areas where ...Antibiotic inhibits cancer gene activity
...loped a new screening system, based on a naturally fluorescent protein called luciferase, to identify small molecules that inhibit proteins that turn genes on and off. Using this system, they identified an antibiotic, siomycin A, that specifically targets FoxM1 without affecting other cell functions. In furthe...OHSU researchers demonstrate how white blood cells cannibalize virus-infected cells
...nse to a systemic viral infection. "If you use a fluorescent dye to stain infected cells, you can literally wat...cently labeled cells, they become labeled with the fluorescent dye themselves," explained Mark Slifka, Ph.D., a researcher in the VGTI who led the research. Slifka...Living view in animals shows how cells decide to make proteins
...mouse. The mouse carried in its FGFR2 gene a green fluorescent tag that would glow when a common type of silencer, called an "intronic silencer," chopped out a specific exon, called IIIb. In this way, the scientists could track whether intronic silencers were chopping out the IIIb exon -- and if so, in which ...Compound eyes, evolutionary ties
...es of the fruit fly (which glow at center due to a fluorescent protein) allows the formation of distinct light gathering units in each of its 800 unit eyes, an evolutionary change to an "open system" that enabled insects to make significant improvements in visual acuity and angular sensitivity. In contrast, beet...Two nerve cells in direct contact
...ively with laser ablation (the cell is filled with fluorescent dye which has a toxic effect when strongly stimulated) and tested sensitivity to rotation in the H2 cell. A long series of these technically very difficult experiments provided unambiguous proof: if the ipsilateral CH cell was destroyed, no effect wa...NHGRI aims to make DNA sequencing faster, more cost effective
...re is used to simultaneously detect electrical and fluorescent signals from many nanopores at one time. A novel s...uencing" This team has developed a novel type of fluorescent nucleotide that is modified for sequencing by synthesis. Their goal is to improve the chemical subun...Transplanted photoreceptor precursor cells restore visual function in mice with retinal degeneration
...ne regulatory element to tag the rods with a green fluorescent protein. This enabled Kellogg scientists to illuminate the rods, which begin as unspecified cells and gradually differentiate into cells dedicated to light reception. "One of our critical discoveries," says Swaroop, "is that there is a window of opp...NYU biologists identify gene that coordinates two cellular processes
...codes. By fusing mel-28 to a gene-encoding GFP, a fluorescent marker, and expressing this fusion in early embryos, they visualized the movement of MEL-28 during cell division in living embryos. Consistent with the idea that MEL-28 had function in chromosome segregation and the nuclear envelope, the MEL-28-GFP f...