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Tag: "fluorescence" at biology news

Cellular message movement captured on video

...oward the nucleus. Paxillin was labeled with a red fluorescence marker to make it stand out in live cells. Video ...ally engineered proteins tagged with red and green fluorescence markers to the bovine aortic endothelial cells, grew them in tissue culture, and filmed them while t...

Envisat captures first image of Sargassum from space

...65, 681 and 709 nanometres in order to measure the fluorescence emission from chlorophyll a. Chlorophyll is the green photosynthetic compound in plants that captures energy from sunlight necessary for photosynthesis. The amount of chlorophyll present in vegetation plays an important role in determining how he...

Brightening prospects of using fluorescent nanotubes in medical applications

... clinical applications.” The figure of merit for fluorescence is quantum efficiency: the ratio of the number of ...propriate for “in vivo” applications. Nanotube fluorescence is extremely stable and can last for months. Fluorescent proteins – widely used for imaging living s...

8 plants from South Africa may hold potential for treating high blood pressure

...metric method with Hip-His-Leu as a substrate. The fluorescence of the o-phthaldialdehyde was measured to determine the effect of the plant on ACE activity. Plasma ACE activity was determined using rat plasma. The IC50 of the conventional ACEI, captopril was determined to test the sensitivity of the assay. At ...

Sandia researchers take new approach to studying how cells respond to pathogens

...c and imaging tools," Singh says. Hyperspectral fluorescence imaging with multivariate curve resolution (MCR) is used to image the tagged proteins and provide quantitative measurements on multiple proteins simultaneously. The goal is to analyze as many as 10 to 40 proteins and cellular stains at a time in thre...

Sheffield scientists light up bacteria

...er bacteria, and developing a light signal through fluorescence non radiative energy transfer (NRET), the researchers will be able to detect early stage bacterial contamination. Being developed by a multi-disciplinary team of researchers from the University’s Departments of Chemistry, Engineering Materials a...

Rapid, Low-Cost DNA Testing

...agged probe adsorbs on a gold nanoparticle and its fluorescence is quenched. If the probe sequence is able to hybr...the target, it will not adsorb on the gold and its fluorescence persists. The new method is simple and effective. It costs very little, and it's very quick. T...

New study sheds light on 'dark states' in DNA

...tate -- so called because it cannot be detected by fluorescence techniques used to study other high-energy states created in DNA by UV light. The study suggests that DNA employs a variety of means to dissipate the energy it absorbs when bombarded by UV light. Scientists know that UV light can cause genetic al...

Scripps research study reveals structural dynamics of single prion molecules

...veral novel technologies including single-molecule fluorescence resonance energy transfer (SM-FRET) and fluorescence correlation spectroscopy (FCS). Fluorescence resonance energy transfer is a highly sensitive too...

The connector rotation hypothesis? Phage do not pack their DNA by rotation

...s packed. The researchers combined single-molecule fluorescence polarization with "magnetic tweezers" to glue the ...hey could observe its motion using single-molecule fluorescence polarization spectroscopy. The researchers then looked at connector movement during DNA packaging...

Cancer cells forming blood vessels send their copper to the edge

...ogy, at the University of Chicago, have used X-ray fluorescence microprobe imaging at the Advanced Photon Source a...ide to side, top to bottom). By collecting emitted fluorescence spectra at each point using an energy-dispersive detector, the researchers obtained images displayin...

New imaging technique tracks traffic patterns of white blood cells

... Cell Institute in New York. "Multichannel digital fluorescence videomicropscopy vastly improves on prior intravital observations of leukocyte behavior in vivo by allowing the near-simultaneous observation of cell surface markers and microdomains." Researchers explored various mouse models including that of Si...

Gene key to taste bud development identified

... In their work, Hogan and her colleagues use this fluorescence marker as a tool for tracking the activity of SOX2 in the esophagus, among other sites. As they worked with the mice, they noticed that specific areas on the tongue and in the back of the mouth lit up, in addition to areas in the esophagus. Further s...

Plant studies reveal how, where seeds store iron

..., genetics and high-resolution 3-dimensional X-ray fluorescence imaging--to understand gene function," said Jane Silverthorne, a program director in NSF's Division of Biological Infrastructure, which funded the research. "The discovery that iron localizes in specific parts of a seed opens the possibility of devel...

Plant studies reveal how, where seeds store iron

..., genetics and high-resolution 3-dimensional X-ray fluorescence imaging--to understand gene function," said Jane Silverthorne, a program director in NSF's Division of Biological Infrastructure, which funded the research. "The discovery that iron localizes in specific parts of a seed opens the possibility of devel...

NHGRI aims to make DNA sequencing faster, more cost effective

...ngle DNA base on an immobilized primer/template. A fluorescence scanner will be used to scan and identify hundreds...emplates in high-density arrays, using a sensitive fluorescence detection system. Steven Jeffrey Gordon, Ph.D., Intelligent Bio-Systems, Inc., Worcester, Mass.,...

Brown scientists map structure of DNA-doctoring protein complex

...methods such as X-ray crystallography. Sun used fluorescence resonance energy transfer or FRET, a technique typically used to study small protein complexes in a solution. This time, Sun used FRET to study large protein complexes in a gel. He tagged the DNA with fluorescent dyes and purified the proteins, placi...

Powerful genome ID method extended to humans

...large-scale structure of a genome. The method uses fluorescence microscopy to image individual DNA molecules that have been divided into orderly fragments by so-called restriction enzymes. By imaging large numbers of an organism's DNA molecules, optical mapping can produce a map of its genome at a relatively l...

An advanced genetic diagnostic method for multiple myeloma

...ition, the technique of hybridization in situ with fluorescence allowed him to identify new recurrent genetic changes that are involved in the appearance of this pathology. A disease associated with old age Multiple myeloma is a disease which primarily affects persons above 60 years of age. In 2001 in Spain, ...

Nanotech tools yield DNA transcription breakthrough

...tudy by the Rutgers/UCLA team used single-molecule fluorescence spectroscopy. The researchers attached pairs of fluorescent "tags" to key structural elements of the machine and then monitored changes in distance between tags in single molecules as transcription occurred. The researchers showed that, during initia...

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