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Tag: "feeder" at biology news

Current human embryonic stem cell lines contaminated UCSD/Salk team finds

...human embryo-derivedconnective tissue cells as the feeder layer in the culture.A further solution might be a short-term culture in heat-inactivatedserum from the actual patient who is going to receive the therapy, thescientists said. However, it may still prove difficult to completelyeliminate the Neu5Gc, b...

Scientists rid stem cell culture of key animal cells

...through the tedious daily task of generating mouse feeder cells from mouse embryos. Feeder cells, or fibrobl... the matrix upon which stem cells grow. The mouse feeder cells were an important ingredient in the mix of culture materials required to keep stem cells in th...

UCSD researchers maintain stem cells without contaminated animal feeder layers

... grown and nourished in petri dish material called feeder layers that are made with animal connective tissue...ures, problems have remained. In some cases, human feeder layers were developed but this added another measure of complexity to the culture system, Hayek note...

Discarded placentas deliver researchers promising cells similar to embryonic stem cells

...ver without needing another cell type serving as a feeder cell layer. This is significant, because to replic...thelial cells, on the other hand, create their own feeder layer, with some cells choosing to spread out at the bottom of the culture dish thereby giving those...

Tissue regeneration operates differently than expected

... they are co-cultured with growth-factor producing feeder cells, finally they did not become entirely functional muscle cells. Fully-functional muscle cells only developed after the mesenchymal stem cells were cultivated together with skeletal or heart muscle cells. This was indicated by the green fluoresc...

Breakthrough for stem cell research

... any way. Our line grows on human fetal fibroblast feeder layer that does not require fetal calf serum," said UNSW Senior Lecturer, Dr Kuldip Sidhu, who was the lead scientist responsible for the creation of the line. "Other researchers have used animal tissue to keep the hESC alive in the petri dish or as...

Wisconsin scientists grow two new stem cell lines in animal cell-free culture

...able to culture stem cells in the absence of mouse feeder cells, the most prominent animal product in stem c...was established in the 1950s. In addition to mouse feeder cells obtained from mouse embryos, the original embryonic stem cell cultures depended on bovine seru...

Scientists re-grow dental enamel from cultured cells

...iferate when they are cultured on top of a special feeder layer of cells (the feeder-layer cells are known a...reen of Harvard Medical School reported the use of feeder layers for culturing epithelial cells from the skin (the 3T3-J2 cells used in the current study were...

Stem cells act through multiple mechanisms to benefit mice with neurodegenerative disease

...compatible with clinical use (i.e., without animal feeder layers) and even without the need for immunosuppre...uman embryonic stem cells were grown without mouse feeder layers and in a "defined" culture medium that is compatible with clinical use and demonstrating for ...

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