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Tag: "dye" at biology news

Scientists directly view immune cells interacting to avert autoimmunity

...een accomplished. The scientists used fluorescent dye to mark different types of cells so they could directly observe the interactions of the key cell types involved in initiating autoimmune attacks and the control of their actions by therapeutic regulatory T cells. The "nonobese diabetic" (nob) mouse ...

UF scientists trace origin of shark's electric sense

...s are marked with dye, the embryo develops and the dye clearly shows up in the electroreceptors." Dye tests are a classical way of mapping cell movements during development, and have been used to explore the origins of limbs and brain cells. In the current research, scientists used genetic markers to tr...

Blood flow in brain takes a twist, affecting views of Alzheimer's

...s in living organisms. The team used a fluorescent dye to light up the blood vessels, then put a special form of the chemical calcium into astrocytes. They used one laser to activate the calcium, and another laser to monitor how astrocytes processed the chemical. They found that astrocytes caused blood v...

Predicting successful outcomes in living-donor liver transplants

...LDLT) found that measuring how a certain non-toxic dye was eliminated by the liver shortly after surgery was an accurate indicator of liver function, and therefore a reliable indicator of the outcome of the procedure. The study used a simple non-invasive device to measure the dye, making it particularly ...

USC researchers investigate protein that protects tumors

...senior author. The scientists used a fluorescent dye attached to the protein's antibody to reveal the protein's location on the tumor cells. "The first step was to identify whether it's there [on cancer cells] and how often," he explained. "We found that it was present on 60 percent of the tumors ?a...

New genes implicated in rheumatoid arthritis

...s of every twin, labelled samples with fluorescent dye to distinguish RA cells from disease-free cells, and hybridized each on a 20,000-gene chip. Then, using immunohistochemistry and real-time polymerase chain reaction, they confirmed the expression of the most significantly over-expressed genes in syn...

'Micro-boxes' of water used to study single molecules

...g process), detecting the fluorescence signal from dye and protein molecules, and observing the transfer of energy from one end of a specially treated DNA molecule to the other. Water offers several advantages over other methods for containing single molecules, such as attaching them to surfaces or pla...

Life and death in the hippocampus: what young neurons need to survive

...s infected with virus were marked by a fluorescent dye enabling detection of neurons derived from those cells. A few weeks later, animals that received the virus showed fewer fluorescent neurons compared to mice injected with a benign virus lacking the shears, meaning fewer new neurons had survived ori...

Recombination protein dynamics observed with single monomer resolution

...eloped. To use FRET, researchers first attach two dye molecules ?one green and one red ?to the molecule they want to study. Next, they excite the green dye with a laser. Some of the energy moves from the green dye to the red dye, depending upon the distanc...

Encoded metallic nanowires reveal bioweapons

...ntibodies that have been tagged with a fluorescent dye are added to the sample. These also dock onto the pathogen so that it is surrounded like the filling in a sandwich; giving the technique its name, "sandwich immunoassay". Measurement of the fluorescence now gives information about the pathogen concen...

Two nerve cells in direct contact

...aser ablation (the cell is filled with fluorescent dye which has a toxic effect when strongly stimulated) and tested sensitivity to rotation in the H2 cell. A long series of these technically very difficult experiments provided unambiguous proof: if the ipsilateral CH cell was destroyed, no effect was se...

OHSU researchers demonstrate how white blood cells cannibalize virus-infected cells

...temic viral infection. "If you use a fluorescent dye to stain infected cells, you can literally watch T...ed cells, they become labeled with the fluorescent dye themselves," explained Mark Slifka, Ph.D., a researcher in the VGTI who led the research. Slifka is ...

The connector rotation hypothesis? Phage do not pack their DNA by rotation

...lso labeled the connector complex with fluorescent dye molecules so they could observe its motion using single-molecule fluorescence polarization spectroscopy. The researchers then looked at connector movement during DNA packaging in six ö29 mutants. After attaching the fluorescent molecules to the co...

Allergy to hair dye increasing

Allergic reactions to hair dye are increasing as more and younger people dye their hair, warn researchers in this week's BMJ. This can lead to dermatitis on the face and, in se...

Newborn neurons like to hang with the 'in' crowd

...s infected with virus were marked by a fluorescent dye enabling the researchers to follow their fate over time as they tried to get accepted into the existing circuitry. With the help of a whole arsenal of high-tech imaging technology and the electron tomography expertise of Mark. H. Ellisman, Ph.D., ...

Technique monitors thousands of molecules simultaneously

...orm a carbonyl and then used the carbonyl to put a dye down. They placed a green dye at alternating electrodes in a checkerboard pattern, and then used the alternate set of electrodes t...

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