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Tag: "cultures" at biology news

Wisconsin scientists grow two new stem cell lines in animal cell-free culture

...y, the ability to now grow embryonic stem cells in cultures free of animal products could accentuate the debat...om mouse embryos, the original embryonic stem cell cultures depended on bovine serum products. The two new Wisconsin stem cell lines were derived from five bla...

Hopkins study suggests commercially available antibiotic may help fight dementia in HIV patients

..., Rumbaugh and colleagues grew human neuronal cell cultures in a lab from existing human neuron cell lines, treated them with a range of doses of ceftriaxone, and exposed them to Tat or gp120. They found that the antibiotic protected the neurons against both HIV proteins. The dose of ceftriaxone needed for pr...

Proteins spur diabetic mice models to grow blood vessels, nerves

...n 2004, Li and his colleagues showed in laboratory cultures that in addition to promoting nerve growth, Netrin-1 also induced formation of blood vessels. ...

Purdue creates new low-cost system to detect bacteria

...cientists in food-processing plants routinely grow cultures to test for dangerous pathogens. "The dairy industry, for example, grows bacteria on petri dishes to make sure products are safe, but industry is trying to develop technologies that will very quickly identify organisms," Robinson said. "The same so...

Synthetic molecule causes cancer cells to self-destruct

...researchers tested the compound's efficacy in cell cultures and in three mouse models of cancer. The testing was performed in collaboration with William Helferich, a professor of food science and human nutrition at the U. of I., and Myung-Haing Cho at Seoul National University. The researchers also showed tha...

Parkinson's disease mechanism discovered

...uitfly Drosophila, the roundworm C. elegans and in cultures of rat neurons. Bonini and her colleagues tested the effect in fruitflies; co-author Guy Caldwell and his colleagues at the University of Alabama performed the tests in roundworms; and co-author Jean-Christophe Roche and his colleagues at Purdue perf...

With few factors, adult cells take on character of embryonic stem cells

...em cells can be directly generated from fibroblast cultures by the addition of only a few defined factors," Yamanaka said. Fibroblasts make up structural fibers found in connective tissue. Embryonic stem cells are derived from inner cells of the mammalian blastocyst, a ball of cells that develops after fer...

Teasing out tissue from blood

...evels of glucose. "We now want to transplant these cultures into a diabetic mouse model," says Winnier, adding their results are being prepared for publication in a peer-reviewed journal. Alan Colman of ES International, a company developing insulin-producing cells from human ESCs in Singapore, is also scept...

Use of stone hammers sheds light on geographic patterns of chimpanzee tool use

...lights the necessity to preserve the rich array of cultures found across chimpanzee populations and communities, which represent our best model for understanding the evolution of hominid cultural diversity. As such, the new finding promises to both benefit research and inform the conservation of our closest l...

How IVF could be causing genetic errors in embryos

...re also used with 20% oxygen, making six different cultures in total. As a control, some embryos were left to mature in the mice (in vivo). "We identified 38 imprinted genes, most of which showed no difference in expression after in vitro culture compared to the in vivo embryos. Five genes showed a statistic...

With few factors, adult cells take on character of embryonic stem cells

...em cells can be directly generated from fibroblast cultures by the addition of only a few defined factors," Yamanaka said. Fibroblasts make up structural fibers found in connective tissue. Embryonic stem cells are derived from inner cells of the mammalian blastocyst, a ball of cells that develops after fer...

K-Staters design and build a low-cost remote sensing tool for environmental studies

...ska," Ham said. "The earth's atmosphere links all cultures and nations. By that, I mean that carbon dioxide emitted in China, for example, can impact the vegetation in North America or Europe and vice versa. Any decisions about climate change have to be based on a complete global analysis," he said. "Having...

'Smart' genetic therapy helps the body to heal itself

...sy patients after surgery, as well as motor neuron cultures derived from rat embryos. "The next step was to try this therapy in patients", said Professor Wirth. "In a first pilot trial, we enrolled ten parents of SMA patients who were treated with valproate for four months. We saw that valproate signific...

Mayo Clinic collaboration mining of ancient herbal text leads to potential new anti-bacterial drug

...n has revived the healing wisdom of Pacific Island cultures by testing a therapeutic plant extract described in a 17th century Dutch herbal text for its anti-bacterial properties. Early results show that extracts from the Atun tree effectively control bacteria that can cause diarrhea, as claimed by naturalist...

MIT creates 3-D scaffold for growing stem cells

... a more conducive microenvironment for tissue cell cultures and tissues used in regenerative medicine, such as...s. "I believe that in the next 20 years all cell cultures will be in 3D with the designer scaffolds, and most textbooks about cell biology will have to be rev...

UGA scientists unravel 'molecular inch-worm' structure of walking-pneumonia bacterium

...rganelle. We've been able to observe it in growing cultures and describe the choreography of events at a level of detail not previously possible." The research is being published this week in The Proceedings of the National Academy of Sciences. Other authors of the paper include graduate student Benjamin Ha...

Restoring tamoxifen sensitivity in resistant breast cancer cells

... found that the effectiveness of tamoxifen in cell cultures and in mice can be fully restored by the use of a compound called disulfide benzamide, or DIBA. The investigators confirmed their study hypothesis about DIBA's effect on tamoxifen resistance by using the compound in mice that were engineered to have ...

Human testis harbors HIV-1 in resident immune cells

... was demonstrated further by using explanted organ cultures in which human testis tissue was grown in culture. This testis culture, which retained the same tissue architecture as in vivo tissue and continued to secrete testosterone, was able to support infection by HIV-1. Virus produced from the testis cultur...

Cancer stem cells linked to radiation resistance

...s extracted cells from the tissue and grew them in cultures in the laboratory. For the second model, they tran...stered set doses of ionizing radiation to the cell cultures and to the mice. In both cases, the researchers observed a roughly fourfold jump in the number of gl...

Parkinson's mutation stunts neurons

...ersion. When they introduced the mutant forms into cultures of neurons, they saw a reduction in the growth and branching of the neurons. Such growth is critical for the neurons to establish and maintain connections with one another in the brain's neural circuitry. The researchers also found that cultured neur...

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