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Tag: "cultured" at biology news

Transplantation Of Monkey Embryonic Stem Cells Reverses Parkinson Disease In Primates

...onic stemcells can differentiate into neurons when cultured under specificconditions. These same culture conditions, technically simple andefficient, were recently applied to primate embryonic stem cells andresulted in the generation of large numbers of DA neurons. In theircurrent JCI study, Jun Takahashi and...

Novel Therapy Tested in Mice Could Chase Away Cat Allergies

... in blood donated by people allergic to cats. They cultured blood cells with either GFD or with a purified human antibody as a control. Then they added the cat protein that triggers allergic reactions to all the blood cell cultures. “We measured more than 90 percent less histamine in the cultures with GFD,?s...

Needling Chromosomes Reveals Cell Division Secret

... needles to impale each of twosister chromatids in cultured grasshopper cells at the appropriate timein cell division. Then, she would ever-so-gently apply force to pullthem apart. Upon release, if they remained apart it revealed they wereseparated; but if they snapped back together the researchers would know...

UCSD researchers maintain stem cells without contaminated animal feeder layers

...human pancreatic cells. Human embryonic stem cells cultured for several weeks under these conditions showed no change in cell form and structure. The team then eliminated each factor and pluripotency was assessed. At first, the results were narrowed to three molecules, with activin A, a protein that participa...

Scientists identify genetic pathways essential to RNA interference

...Although the process has worked well in studies of cultured human cells, it has not yet been effective for experimentally suppressing gene expression in living mammals. Identifying each step in the RNAi process could lead to more successful inactivation of disease-related genes. And in addition to the techniq...

Studies reveal methods viruses use to sidestep immune system

...ses when the hepatitis C virus (HCV) replicates in cultured human cells. Those immune defenses should fight off the virus, but in a separate study, scheduled to appear online this week and in an upcoming issue of the Proceedings of the National Academy of Sciences, the researchers show how HCV sidesteps the ...

Newly Discovered Compound Blocks Known Cancer-Causing Protein

...e scientists showed they could block the growth of cultured colon cancer cells using this new compound, called cysmethynil. Their finding, reported in the March 22, 2005, issue of Proceedings of the National Academy of Sciences, is the first step toward developing a new class of anti-cancer drugs that block ...

PCRM develops world's first cruelty-free insulin assay

...use kits containing antibodies produced from cells cultured with fetal calf serum. Neither was acceptable to us." The answer? Develop an in-vitro, or test-tube, procedure using a synthetic replacement for the fetal calf serum used as a culturing medium in millions of medical tests every year. After months o...

Molecular Motors Cooperate In Moving Cellular Cargo, Study Shows

...that break down toxic substances) inside specially cultured fruit fly cells. This was the first time the imaging technique had been used inside a living cell. "Our measurements show that both dynein and kinesin carry the peroxisomes in a step-by-step fashion, moving about 8 nanometers per step," said Selvin,...

Scientists identify genetic pathways essential to RNA interference

...Although the process has worked well in studies of cultured human cells, it has not yet been effective for experimentally suppressing gene expression in living mammals. Identifying each step in the RNAi process could lead to more successful inactivation of disease-related genes. And in addition to the techniq...

Protein That Promotes Survival Of Stem Cells Might Be Key To Poor Leukemia Prognosis

... Mcl-1 did not multiply when removed from mice and cultured in the laboratory. However, BM cells with the gene continued to flourish. In contrast, liver cells were unaffected following loss of Mcl-1, demonstrating that Mcl-1 is important only in certain cell types. Finally, the investigators showed that growt...

Two chemicals boost immune cells' ability to fight HIV without gene therapy

...e cells from the blood of HIV-infected persons and cultured the cells with the chemical compounds. They were surprised to see that the compounds produced the same three changes in the cells as those created by the gene therapy. "Lo and behold, we discovered we didn't need to use gene therapy to reactivate th...

Molecular thermometers on skin cells detect heat and camphor

...atapoutian and his colleagues also showed that, in cultured keratinocyte cells, TRPV3 is activated by the compound camphor, which is one of the main ingredients in many warming rubs. TRPV3 is the first known receptor for camphor. Significantly, when Patapoutian and his colleagues discovered TRPV3 a few years...

Sugar beet virus mutation requires Texas touch

...the infected plants, he said. The virus isolate is cultured in test tubes, and the plants are no longer needed. "We're trying to find out why this genetically mutated pathogen is able to overcome the resistance in the plant," he said. "We're looking at new crosses by seed companies and challenging them with ...

Human Eggs Can Develop From Ovarian Surface Cells In Vitro

...rst time that human eggs may develop directly from cultured ovarian surface epithelium (OSE) cells derived fro...t human eggs and granulosa cells) can develop from cultured OSE cells. By scraping cells from the surface of adult ovaries and growing them for 5 to 6 days in t...

Gene silencing technique offers new strategy for treating, curing disease

... how they efficiently shut down gene expression in cultured cells by blocking the ability of chromosomal DNA to be copied into RNA and made into proteins. The studies, which Dr. Corey said represent the most significant findings thus far in his career, are the most definitive to date showing that chromosomal ...

Cancer related gene p53 not regulated as indicated by previous tissue culture research

...l now, scientists had assumed, based on studies in cultured cells, that p53 had to be modified by attaching chemical groups to specific sites on the protein to function normally in the body. The new research indicates that these modifications are not necessary to activate p53 under conditions of stress or to ...

Random gene expression may drive HIV into hiding

...s heads for latency. When the scientists infected cultured human cells with their viral vector, they found that cells that initially expressed a low level of virus were very unstable: After a few days, all cells expressed either a high level of virus or none at all. When Weinberger took one infected human c...

Human embryonic stem cells have the potential to develop into eggs and sperm in the laboratory

...ore difficult, because we have to recreate for the cultured cells the environment of the developing follicle for egg development or the tissue of the testis for sperm. We want to test whether HESCs can differentiate to cells that produce the hormones for sperm and egg development and isolate these as well. Wh...

New protein vital for immune response is found in surprise location

...e Sept. 9 issue of the journal Cell. Working with cultured cells, researchers found that the protein, made by a gene they discovered and named MAVS, is located in an unexpected place within the cell ?in the membrane of an organelle called the mitochondrion, which until now was best known for generating energ...

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