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Tag: "culture" at biology news

Liver cancer linked to cellular repair pathway

...romoting cancer development. In test tube and cell culture experiments, blocking the Hedgehog pathway reduced growth of cancer cells by over 90 percent. Liver cancer, or hepatocellular carcinoma, often develops in people with cirrhotic livers damaged by chronic infections, such as hepatitis, by alcohol abus...

New journal article urges use of animal serum-free media for growing live cells

..., enabling scientists and researchers to make cell culture safer and more humane. "Scientists have access to humane and scientifically superior alternatives, so now is the time to completely eliminate the use of fetal calf serum in the laboratory," says Megha Shah Even, M.S., a staff scientist at PCRM and ...

Carbon nanotube absorption measured in worms, cancer cells

... that about 74 percent of the nanotubes added to a culture of cancer cells were assimilated by the cells after 15 minutes, and 89 percent of nanotubes assimilated after six hours, according to the paper. And the uptake was nearly irreversible, with only about 0.5 percent of the nanotubes releases from the ce...

Driver of anthrax toxicity could lead to late-stage therapy

...cellular domain of LRP6 can protect cells grown in culture against killing by anthrax toxin suggest that the immunological targeting of LRP6 may prove useful in protecting against the effects of accumulated toxin during the late stages of anthrax disease when antibacterial methods normally are no longer of t...

Clues to breast cancer hidden inside stem cells

...ow much a cells context matters, used the 3-D cell culture pioneered by Mina Bissell, at the Lawrence Berkeley National Laboratory in California. "We can build up a 3-D breast structure similar to what you have in vivo," says Gudjonsson. "You can analyse cell-cell interactions and signaling pathways in the...

Testes to incubate stem cells

...o sperm, said de Rooij. "Our plan is to develop a culture system for spermatogonial stem cells" de Rooij tol...iding useful insights. "We'd like to know how to culture human spermatogenic stem cells to restore male fertility after cancer therapy," says Hannu Sariola, ...

Researchers release new variety of asparagus

...parent plants are produced by cloning using tissue culture methods. The male and female plants are then planted in an isolated field. Bees pollinate the females, leading to seed production. These seeds are then sold and planted by farmers who produce the spears consumers eventually will buy. "The first comme...

UCI researchers discover key factor for survival of human embryonic stem cells

...solution to the problem. By treating stem cells in culture with chemicals that block the action of neurotrophins on hES cells, Donovan said, scientists can kill the undifferentiated stem cells before they are implanted into the body. According to Donovan, the studies also offer further proof that new stem ...

New influenza vaccine takes weeks to mass produce

..., and are 100% effective in the prevention of cell culture confirmed influenza. Wang and his colleagues report the successful production of rHA from 4 strains of influenza that scientists believe to be likely the cause of the next pandemic (H5, H7, H9, and H2) at a level where manufacturing costs are expec...

Gene patterns in white blood cells quickly diagnose disease

...d of days that would be required using traditional culture methods. "The technology is not there yet, but within 10 years this test could also be done in doctors' offices for a variety of common illnesses, including some types of cancer," says Hammamieh. ...

Stem cell study for patients with heart attack damage seeks to regenerate heart muscle

...c patient. Dr. Schaer says the cells are grown in culture to very high numbers, allowing a single donor's cells to treat thousands of patients. "The cells have the ability to expand, or multiply, under controlled conditions, and the expanded cells have the ability to develop into different types of cells in...

Salmonella caught red-handed

...ttack, according to genome analysis and laboratory culture experiments ?but validation in in vivo infection models is largely lacking. Infection biologists and proteomics researchers have now identified all the proteins involved in Salmonella metabolic paths during an infection. Dirk Bumann of Hannover Med...

Findings advance use of adult stem cells for replacement bone

...rs at Rice University have discovered a new way to culture adult stem cells from bone marrow such that the cells themselves produce a growth matrix that is rich in important biochemical growth factors. The research, which appears online this week in the Proceedings of the National Academy of Sciences, is no...

New analysis shows three human migrations out of Africa

...s the adaptation and expansion of a new stone tool culture first found in Africa and later at 700,000 years expanded throughout Eurasia. "By the time you're done with this phase you can be 99 percent confident that there was recurrent genetic interchange between African and Eurasian populations," he said. ...

Measuring artery repair cells could become new heart disease test

... the immune system cells from a blood sample, then culture the remaining cells and then count individual of EPC colonies that grow. The new system permits researchers to gauge the number of EPCs based by identifying those cells with a high amount of ALDH activity. The Duke researchers based their studies on...

Variation in bitter-taste receptor gene increases risk for alcoholism

...eople at an increased risk for alcoholism. In cell culture experiments, Goate found that the variant receptor...ensitive to bitter tastes as suggested by the cell culture experiments. As part of this investigation, Goate's team took advantage of available genome sequen...

Uncovering sex-change secrets of black sea bass

...position. "Black sea bass is a wonderful fish to culture and to eat," says George Nardi, vice president and director of GreatBay Aquaculture, a commercial fish farm in Newington, NH. But the sex change problem must be tackled if fish farmers are to bring a high-quality fish to market. "We invest in our bro...

Liquid crystals show promise in controlling embryonic stem cells

...s, the team reports on a liquid crystal-based cell culture system that promises new ways of achieving real-ti...nges of liquid crystal texture and color, our cell culture system is able to report, in real time, the cell interactions with the underlying support on which t...

Study indicates dramatic rise in antibiotic-resistant community-acquired staph infections

...he diagnosis by obtaining appropriate material for culture in order to achieve a definitive diagnosis and ensure appropriate treatment is given. At Grady Memorial Hospital, where the study took place, Dr. Blumberg reports that his team has already started following up on the findings: "We have worked hard o...

Engineered heart tissue offers insights into irregular heartbeats, defibrillator failure

Engineers who have induced heart cells in culture to mimic the properties of the heart have used the... of neonatal rats. They transferred the cells into culture dishes on which they had stamped precise patterns of proteins known to support heart tissue growth. ...

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