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Tag: "culture" at biology news

Researchers learn how blood vessel cells cope with their pressure-packed job

...ial cells from the bovine aorta, grew the cells in culture flasks, and seeded them onto the silicone membranes. After the cells grew into confluent layers, a piston-like "indenter" was programmed to repeatedly push into the underside of the membranes and retract. The 60-cycle-per-minute motion of the indente...

New factor implicated in allergy and asthma attacks

...e produce damaging reactive oxygen species in cell culture and, in experiments with mice, in their lungs and airway lining fluid. The resulting oxidative stress, Boldogh said, almost immediately prompted the production of inflammatory immune signaling molecules and accumulation of inflammatory cells, a downs...

Embryonic stem cells accrue genetic changes

...ays Chakravarti. "Whenever you have something in a culture dish, it can change, and it will be important to identify, keep track of and understand these changes." At this point, the precise effects of these changes on the cells aren't known, but some of the changes resemble those seen in cancerous cells. A...

Life in deadly conditions

...s and amino acids. Thus, the growth medium for the culture of the single-celled organism could be significantly simplified. Dieter Oesterhelt explains that "the comparison with other halophile archaea we have studied shows that these organisms have a high plasticity with which they can adapt to the varying,...

War on terror meets war on cancer

...rature in flasks containing a nutrient-rich liquid culture medium. There were four sets of flasks, each holding a growth medium containing water with a different ratio of oxygen-18 to oxygen-16. After the bacteria grew for three hours, the contents of each flask were sucked through a filter, leaving a pasty...

Stem cell microenvironment reverses malignant melanoma

... these melanoma cells were less invasive following culture on the microenvironments of hESCs. "Our observations highlight the potential utility of isolating the factors within the hESC microenvironment responsible for influencing tumor cell fate and reversing the cancerous properties of metastatic tumor cel...

Genetic testing still smart choice, despite uncertainties

...f mosaicism, the UF researchers suggest. Improving culture conditions could help too, Kovalinskaia said. But the small margin of error shouldn't stop couples from having preimplantation genetic diagnosis, said Jamie Grifo, M.D., Ph.D., a professor of obstetrics and gynecology at New York University. The tes...

Sperm stem cells closer to being like embryonic stem cells

...is, for example ?by identifying and eliminating in culture a man's sperm stem cells that carry the gene. Dr. Garbers said that a renewable source of cultured sperm stem cells, rat or human, also could be used to test for male-directed contraceptives, and a company is already interested in this possibility....

Bare metal stents deliver gene therapy to heart vessels with less inflammation in animal studies

...eported their proof-of-principle study, using cell culture and animal models, in the early edition of the Proceedings of the National Academy of Sciences, published online this week. "This is the first study to demonstrate successful delivery of a gene vector from a bare metal surface," said senior author R...

New book highlights world's borderless conservation areas

...ct the world's biodiversity and works to promote a culture of environmental awareness within our communities and our society at large." CI President Russell A. Mittermeier, Agrupacion Sierra Madre President Patricio Robles Gil and CI staff and volunteers including Christina G. Mittermeier, Cyril Kormos, Tre...

Greasing interferon's gears may pave way to greater therapeutic benefits, fewer side effects

...ched. Investigations conducted on cells growing in culture showed that the altered Stat1 proteins reacted more efficiently to the presence of both type I and type II interferons. Further tests revealed that the souped-up Stat1 recruited more of a specific protein it needs to pass on the interferon signal, es...

New methods offer insight into regulatory DNA

...e in the cell cultures. If gene activity in a cell culture was skewed from the average, it was investigated further. These genes were correlated with more than 750,000 SNPs ?sequence differences between individuals in the sample collection. A series of statistical tests were carried out to provide increased...

OHSU discovery sheds light into how stem cells become brain cells

... isolated neural stem cells, placed them into cell culture and then removed Brg1, the cells in the culture turned into neurons but failed to differentiate into glia. "This research shows us that in mice, B...

New evidence supports century-old theory of cancer spread

...Their behavior was astonishing," said Pawelek. "In culture dishes, fused cells were extraordinarily motile compared to unfused melanoma cells. They spread rapidly when implanted in mice. Even though the idea is virtually unknown to cancer researchers today, many scientists worked on it in the 20th century. F...

How the neuron sprouts its branches

... And importantly, said Ehlers, the studies in cell culture revealed that the Golgi orientation preceded the preferential growth of long dendrites. "This finding showed us that we weren't just seeing a correlation between Golgi and longer dendrites," said Ehlers. "Initially, when these growing dendrites are ...

Technique offers new view of dynamic biological landscape

...ity. Since yeast form round colonies when grown in culture dishes, they could measure the mutant cells' colony size in an automated fashion and use that to calculate their growth rates. To determine epistatic effects, they compared the growth rate for each cell containing mutations in two genes with the grow...

Researchers develop new method to help find deadly malaria parasite's Achilles heel

... another, modified his technique and added special culture conditions to enable his group to study Plasmodium. Fields's team and collaborators at Prolexys Pharmaceuticals of Salt Lake City, UT, discovered 2,846 interactions involving 1,312 Plasmodium falciparum proteins. The team provided data on those inter...

Forgotten by evolution?

...ed was that they did not add growth factors to the culture medium; the goal was to prevent the cells from differentiating prematurely. By doing so, the scientists discovered that the two cell lines were different in the expression of typical stem cell markers. The researchers thus suspect that mesenchymal st...

Breakthrough for stem cell research

...e to keep the hESC alive in the petri dish or as a culture to grow it on," said Dr Sidhu. "Those animal products have the potential to transmit retroviruses in humans, which could have disastrous consequences." Human embryonic stem cell lines are derived from specialized cells from embryos donated by infer...

Living taste cells produced outside the body

... rat taste buds and placed these cells in a tissue culture system containing nutrients and growth factors. In... many years to maintain taste cells in a long-term culture system, it was commonly believed that these cells could not be kept alive for longer than about 10 d...

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