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Tag: "crystallography" at biology news

RNA enzyme structure offers a glimpse into the origins of life

...rystals of the ribozyme so that he could use x-ray crystallography to determine its structure. Crystallizing RNA molecules is extremely difficult, and Robertson tried dozens of different versions of the ribozyme under different conditions before he succeeded. Using x-ray crystallography--which involves shining a bea...

Chemical switch triggers critical cell activities

...ite with nothing to do and nowhere to go. An X-ray crystallography image could then capture that moment. ...

Nobel Laureate finds 'elegant' explanation for DNA transcribing enzyme's high fidelity

...of the polymerase II transcribing complex by X-ray crystallography in 2001. Those structures revealed the complex with a nucleotide still in the enzyme’s addition site, just after it had been added to the RNA transcript. Later X-ray structures revealed the transcribing complex with the addition site available for ...

Biologists probe the machinery of cellular protein factories

...crystals and Korostelev and Laurberg performed the crystallography and solved the structure, Noller said. ...

Researchers discover how bacteria sense their environments

...tants or explosives. Using a combination of X-ray crystallography to determine the structure of receptors and enzymes and a novel spectroscopic technique for measuring interactions between them, Crane's group was able to develop a model for how the complex of receptors is organized. Jack Freed, Cornell professor of...

Mapping the protein world

In the early days of X-ray crystallography obtaining a three-dimensional model of a protein required wire models, screws, bolts and years of tedious calculations by hand. Today macromolecular models are built by computers ?thanks to sophisticated software and in particular a package called AR...

Molecular DNA switch found to be the same for all life

...nchrotron. With the data gathered at this protein crystallography facility, Berger and his team assembled a high-resolution model of the molecular structure of a protein known as DnaA, which is a member of the AAA+ family. While it has long been known that DnaA controls the process of initiating DNA replication in...

Researchers uncover new mechanism of tumor suppressor

...eld Nuclear Magnetic Resonance spectroscopy, X-ray crystallography and other biochemical and biophysical approaches to elucidate three-dimensional atomic-resolution structures and dynamics of proteins to better understand their physiological functions and relevance to diseases. ...

Oxidation defense in mosquitoes benefits malaria parasite

...have collaborated since 2003. "The use of protein crystallography in combination with biochemical studies and medicinal chemistry, represents a highly multidisciplinary approach that could lead to the identification of novel agents for the treatment of malaria," said Rizzi. The PNAS article describes what an Inter...

Scientists make first step towards 'holy grail' of crystallography

...e the structures of thousands of proteins. Protein crystallography plays a major role in this understanding because proteins, being the major machinery of living things, are often targets for drugs. To direct the proteins to become crystals, researchers use a substance called a nucleant, which does this by encoura...

Major WHO study concludes calcium supplements can reduce complications during pregnancy

...dition to a site that was known earlier from x-ray crystallography studies." These new inhibitors are moving into phase III human trials, he said. For his first round of simulations, Perryman chose a rare double-mutant protease enzyme with two mutations in the 99 amino acids that make up each half of the enzyme. On...

Computer simulation hints at new HIV drug target

...dition to a site that was known earlier from x-ray crystallography studies." These new inhibitors are moving into phase III human trials, he said. For his first round of simulations, Perryman chose a rare double-mutant protease enzyme with two mutations in the 99 amino acids that make up each half of the enzyme. On...

Key heart and Alzheimer's disease protein imaged for first time in native state

...istry. "This is the first successful use of x-ray crystallography to reveal the structure of a protein bound to lipi...th cardiovascular and neurological disease. X-ray crystallography is a technique for determining the three-dimensional structure of a molecule by analyzing the x-ray ...

Advanced genomics and proteomics improve the diagnosis and treatment of a deadly lung disease

...ornia, Berkeley. Doudna's research team used x-ray crystallography to assemble a detailed three-dimensional picture o...rk was facilitated by access to the HHMI-supported crystallography beam lines at the Lawrence Berkeley National Laboratory's Advanced Light Source. The synchrotron at ...

Molecule does more than slice and dice RNA

...ornia, Berkeley. Doudna's research team used x-ray crystallography to assemble a detailed three-dimensional picture o...rk was facilitated by access to the HHMI-supported crystallography beam lines at the Lawrence Berkeley National Laboratory's Advanced Light Source. The synchrotron at ...

MIT chemist discovers secret behind nature's medicines

... Associate Professor Catherine L. Drennan's recent crystallography sleuthing, the secret to the enzyme's enviable pro... the study, crystallized SyrB2 and then used X-ray crystallography to discover the physical structure of the protein. The X-rays scatter off the crystal, creating patt...

Zooming in on the protein-conducting channel

...tate, and one in a non-translocating state. X-ray crystallography data from the lab of HHMI investigator Tom A. Rappaport suggested that the halves of the PCC clamshell were joined in a back-to-back arrangement. However, said Frank, x-ray crystallographic structures often do not represent the arrangements of protei...

Seaweed yields new compounds with pharmaceutical potential

...s of compounds. Finally, researchers used X-ray crystallography (work done at Emory University in Atlanta), nuclear magnetic resonance spectroscopy and mass spectral analyses to determine how carbon, oxygen, bromine and hydrogen atoms connected to make up the molecular structures of the 10 new natural products. ...

UW-Madison scientists zero in on drugs' sweet spots

...Screening Facility; and Ilia A. Guzei, director of crystallography in the UW-Madison department of chemistry. ...

Rutgers to lead $52.7 million protein research program

...y human protein structures, using methods of X-ray crystallography and nuclear magnetic resonance (NMR) spectroscopy, with the full PSI producing between 4,000 and 5,000 over the next five years. "These structures will have tremendous value in understanding basic biology, and in developing the next generation of dia...

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