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Tag: "confocal" at biology news

The transparent organism: EMBLEM and Carl Zeiss give labs a unique look at life

...nology will form a perfect match with our lines of confocal and multiphoton 3D-imaging systems," says Dr. Simon. The agreement between EMBL and Carl Zeiss includes a common cooperation project for method optimization and product development. ...

Confocal imaging promises early detection of skin cancer

...sis,?says Dr Sheblee. The original idea for using confocal microscopy to look deeper into skin came from Germ... Because it calls on lasers and high-end imaging, confocal technology is expensive. One project goal was to reduce the manufacture costs ?by stripping away eve...

'Cookbook recipes' would cure disease with nontoxic DNA delivery systems

...Using synchrotron small angle X-ray scattering and confocal microscopy, Wong and his colleagues -- former Illinois graduate student Hongjun Liang (now a postdoctoral researcher at the University of California at Santa Barbara) and theoretician Daniel Harries at the National Institutes of Health -- investigate...

New technology shows our ancestors ate…everything!

...s," to analyze fossilized tooth surfaces through a confocal microscope, which allows three-dimensional analysis of an object. "You put the specimen in and the microscope is programmed to step down at fine intervals, perform its series of scans, and collect 3D coordinates for each data point," said Teaford. Th...

New, unique microscope for nanotech

...of an atomic force microscope and a laser scanning confocal microscope, the first commercial machine of its ki... built by Asylum Research of Santa Barbara and the confocal microscope system by Olympus America Inc. Integration of the two systems was carried out by Asylum R...

Picking particles faster than one at a time

...with work initiated by Adiga and his colleagues in confocal image analysis, electron microscopy of cell sections, and electron tomographic image analysis, he says that being able to model the whole range of morphological and functional changes in cellular constituents, from the microscale (millionths of a met...

Lactic acid not athlete's poison, but an energy source - if you know how to use it

...s used. Peering at skeletal muscle cells through a confocal microscope, the two scientists saw these proteins sitting together inside the mitochondria, attached to the mitochondrial membrane, proving that the "intracellular lactate shuttle" is directly connected to the enzymes in the mitochondria that burn la...

Researchers solve mystery of how nuclear pores duplicate before cell division

...nother experiment, the group used four-dimensional confocal microscopy to follow the formation in cultured human cells of a single pore that had been labeled with a fluorescent tag. If the nuclear pore had split to give rise to two daughter pores, two bright dots would have emerged from one; however, the rese...

Sea coral's trick helps scientists tag proteins

...d. In fact, the light source of the laser-scanning confocal microscopes that researchers commonly use to peer into living cells can activate the tag, which should make the new tool useful to many scientists, said Sergey Lukyanov, whose lab reports on the Dendra tag in the April 2006 issue of Nature Biotechnol...

Inside rocks, implications for finding life on Mars

...th, Schopf said the techniques he has used, called confocal laser scanning microscopy and Raman spectroscopy, ...roscopy) gives it to you. You see the cells in the confocal microscopy, and the Raman spectroscopy gives you the chemistry. "We can look underneath the fossil,...

FSU biologists uncover mechanisms that shape cells for better or worse

...e" -- relied heavily on the state-of-the-art laser confocal microscope in FSU's Biological Science Imaging Resource facility. Funding for the research came in part from the American Heart Association. ...

3-D model reveals secrets of metastasis

... worked with one sample at a time, using a special confocal microscope at the Whitehead-MIT BioImaging Center. The microscope divided each specimen into virtual slices, generating a new stack of images every 15 minutes. "It took me about a year to get enough data because the microscope wasnâ?super>TM t ...

Ring-like formations in drying DNA drops could affect hybridization studies

...oped at Kent State University. Called fluorescence confocal polarizing microscopy, the technique imaged the DNA in the drying droplet in three dimensions. "The DNA alignment parallel to the droplet's edge was counterintuitive," Wong said. "We had expected the DNA to extend along the direction of flow, which ...

OHSU study says stem cell 'fusion' occurs in tumors

...rs. A second test confirmed colocalization using a confocal microscope, which can scan a single cell layer of intestinal cells for the presence of both the green fluorescent protein, indicating female donor cells, and an enzyme from the male recipient cells Wong said, "Our paper is unique because it's the fi...

Biofuel cells without the bio cells

... FCS, or fluorescent correlation spectroscopy, and confocal microscopy. These yielded a "fluorescence intensity trace" whose brightness depended entirely on whether hematite was available to bind with OmcA in solution. No hematite, dim; hematite, bright. How bright? "The peak current, or flux, doesn't ru...

World's smallest cancer detection device

... cancer." Dr. Condeelis has used the multiphoton confocal microscope to directly observe cellular interactions in the tumor microenvironment of live animal models of breast cancer. By placing an artificial blood vessel near tumors, he was able to collect motile cancer cells for study and to predict--by the ...

A fisheye view of the deadliest breast cancer

...ish vasculature using high-resolution, multicolor confocal microscopy. While the parental MDA cells formed tightly packed aggregates, RhoC-overexpressing cells scattered within the fish tissue. RhoC overexpression also increased tumor cell membrane dynamics leading to continuous shedding of small cellular f...

Sandia researchers take new approach to studying how cells respond to pathogens

...we are taking many work-horse technologies such as confocal microscopy, flow cytometry and immunoassays and combining them into one compact, miniaturized platform using our unique microfluidic and imaging tools," Singh says. Hyperspectral fluorescence imaging with multivariate curve resolution (MCR) is use...

Fast and slow -- How the spinal cord controls the speed of movement

...um ions flooded in as the nerve cells activated. A confocal microscope with lasers allowed the researchers to image the cells at very high resolutions. Using this set up, they watched nerve cells light up as the animals moved at different speeds. While no one knows how this pattern relates to other vertebr...

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