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Tag: "cloning" at biology news

Human genetic 'deserts' are teeming with significant life

...r disease-causing mutations, and they could impair cloning strategies that try to find genes implicated in these pathologies.” They also suggest that extra caution should be used when associating a genetic phenotype with a gene knock-out or knock-in, where genes are added or deleted from a model organism ...

Master regulatory gene of epithelial stem cells identified

...s research team then used the epithelial stem cell cloning methods developed by Howard Green, MD, the George Higginson professor of cell biology at HMS, to show that p63’s key function was to provide the enhanced potential of stem cells to divide. "The fact that p63 is essential for these epithelial ste...

Scientists clone mice from adult skin stem cells

... achieved." Greco, Guasch and Li compared the cloning efficiency of adult skin stem cells with that of m...reports on using adult stem cells for reproducible cloning of mice that survive until adulthood. By using cells from the same mouse and performing the experime...

Novel antigen-cloning technique may boost efforts to develop a melanoma vaccine

...r T cell. Already, Herlyn's group has used the new cloning technique to identify a new tumor antigen called ribosomal protein L8, or RPL8. Findings on the new cloning method and the newly identified tumor antigen will be published as a Priority Report in the April 15...

Penn researchers replace organ in adult mice using 'single-parent' stem cells

... less controversial alternative to the therapeutic cloning of embryonic stem cells. "Creating uniparental embryonic stem cells is actually much more efficient than generating embryonic stem cells by cloning," said K. John McLaughlin, an assistant professor in Penn's Department of Animal Biology and resear...

Cloned mice created from fully differentiated cells, a milestone in cloning research

...volved to a specific type not only can be used for cloning purposes, but they may be a better and more effici... variety of diseases and conditions. Yet, even for cloning of an embryo to the blastocyst stage, from which embryonic stem cells can be generated, adult stem c...

Cloning techniques produce FDA-approved antibiotic

... of bacteria, but generally in low yields. Using a cloning method developed by Illinois microbiologist William W. Metcalf, the researchers were able to clone the essential genes for fosfomycin synthesis and then produce it in a non-native host, potentially in much larger quantities. After isolating the gen...

P(acman) takes a bite out of deciphering Drosophila DNA

...stacles that have hampered research. It allows the cloning of large pieces of DNA to be used to transform the genome, and it permits that DNA to be inserted into specific places in the genome. Bellen credits the report’s first author, Koen J.T. Venken, a graduate student in the BCM Program in Developmental B...

Ten years later, Dolly is still making headlines

...Lisack, AAPS executive director. "With topics like cloning and biogenerics headlining this year's event, there is no more exciting and challenging time to be in the field of pharmaceutical sciences than now." The following topics will headline this year's meeting: Creator of "Dolly" and world-renowned s...

Researchers discover which organs in Antarctic fish produce antifreeze

...ssues in all notothenioids tested. The use of cDNA cloning and sequencing showed that the mRNA all encode secreted AFGPs. An RNA analysis from tissues of a single notothenioid unveiled the anterior portion of the stomach, next to the esophagus-stomach junction, as being the only other site with strong AFGP ...

In a technical tour de force, Salk scientists take a global view of the epigenome

...makeover mature cells must undergo for therapeutic cloning purposes will likely involve remodeling methylation patterns to resemble those of stem cells. And disease states, ranging from retardation to cancer, are marked by aberrant methylation: some carcinogens cause cancer by methylating the "wrong" genes, ...

Genetically engineered mosquitoes show resistance to dengue fever virus

...nd speed up the process. They accomplished this by cloning a section of the virus' RNA and injected two inverse copies of it into mosquito embryos. The copies formed a double-stranded RNA, which, as expected, bound with dicer-2 and was chopped up. The virus never had the opportunity to replicate. As a result...

Cloned stem cells prove identical to fertilized stem cells

...nown as somatic cell nuclear transfer, therapeutic cloning is a promising approach to create individually customized cellular therapies for treating certain disorders. Demonstrated in mice but not in humans, it begins with stem cells derived from a cloned embryo. But if cloned embryos can't produce normal or...

Researchers release new variety of asparagus

... the male and female parent plants are produced by cloning using tissue culture methods. The male and female plants are then planted in an isolated field. Bees pollinate the females, leading to seed production. These seeds are then sold and planted by farmers who produce the spears consumers eventually will ...

Successful cell engineering may lead to mad cow prevention, say researchers

...n England. "The next step is to try and avoid the cloning process - to skip that step if possible in develop...rom here." Westhusin has been involved in several cloning "firsts," among them the first cloning of a cat in 2002 and a white tailed deer in 2004. ...

Flesh-eating bacteria escape body's safety net

...of the DNA-degrading Strep enzyme was confirmed by cloning the corresponding gene into a normally non-pathogenic bacterial strain. Addition of the single gene allowed these bacteria to degrade DNA, escape neutrophil killing, and produce a spreading ulcer in the mouse infection model. Special fluorescent mi...

UF scientists reveal ancient origin of vertebrate skeleton

...te student in zoology, the research team used gene cloning and gene sequencing analysis to demonstrate two type II collagen genes are expressed during the development of the lamprey's cartilaginous skeleton. In addition, they isolated a gene called Sox9, a regulator of collagen gene activity in vertebrates. ...

UF scientists reveal ancient origin of vertebrate skeleton

...te student in zoology, the research team used gene cloning and gene sequencing analysis to demonstrate two type II collagen genes are expressed during the development of the lamprey's cartilaginous skeleton. In addition, they isolated a gene called Sox9, a regulator of collagen gene activity in vertebrates. ...

Enzyme affects hypertension by controlling salt levels in body

...tern Graduate School of Biomedical Sciences. After cloning the enzyme, Dr. Cobb's research group examined what proteins might be regulated by WNK1, and found that SGK1 was one of those proteins. Because he works on ion channels, including sodium channels, Dr. Huang joined efforts with Dr. Cobb to study how W...

AACR Favors 'full spectrum of stem cell research biology'

... replenish damaged tissue. In light of the recent cloning of patient-specific human embryonic stem cells by researchers in South Korea, AACR Science Policy and Legislative Affairs Committee Chairman William G. Nelson V, M.D., Ph.D., pointed to the clause in the AACR position statement encouraging grant-maki...

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