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Tag: "assay" at biology news

WHO data confirms low level of resistance to Tamiflu

... collected were tested by neuraminidase inhibition assay (IC50 or sequence analysis) to detect mutations associated with drug-resistance. Results were as follows: Influenza Season in Japan No of Influenza A isolates tested Resistance No of Influenza B isolates tested Resistance 2003/2004 1180 3 ...

Baumann Lab defines proteins that distinguish chromosome ends from DNA double-strand breaks

...t Human Telomeric DNA Ends" employed a biochemical assay for double-strand break repair to define the minim...k," said Dr. Baumann. "By establishing an in vitro assay for chromosome end protection and by implicating specific proteins, we have opened the door to eluci...

DNA-damage test could aid drug development

...matically cheaper than existing animal tests. This assay would allow genetic toxicity to be examined far ea...ch injects the compound into a live mouse, the new assay is a cell-culture system that could allow hundreds or thousands of tests to be performed from the bo...

Rapid, Low-Cost DNA Testing

... sequences as well as other DNA sequences, and the assay filters out only the targets. Professor Rothberg's assay is based on the electrostatic properties of DNA. The principle underlying the method is that sing...

Cancer tip -- Nanoparticles can damage DNA, increase cancer risk

...using the alkaline single-cell gel electrophoresis assay (Comet assay) to quantify breaks in single and double stranded DNA. The team chose these particular nanoparticle types because they are commonly used commercially ?in electronics, textiles and sporting goods ?and easy to work with in the laboratory ...

Triplex assay used to assay duplex genomic DNA

...icle they present a wealth of data relating to the assay of pathogens in samples also containing human genomic duplex DNA and to the assay of SNPs present in human genomic samples. The assays are carried out homogeneously and in solution a...

New test for most virulent HPV strains under study

...t cervical cancers is under study. The molecular assay uses a cervical scraping, like that for a liquid-b...., in Madison, Wis., is being tested along with an assay that looks for the presence of 14 types of cancer-causing HPV. A test that detects 13 types of HPV a...

Clues to gene expression in cystic fibrosis will guide research

... stop mutation. The team used a dual reporter gene assay first in vitro and then in CF patients. The study found that a small subgroup of patients with the Y122X mutation, found mainly in inhabitants of Reunion island, responded to gentamicin treatment. Cystic fibrosis is caused by mutations in the gene...

Statins stop hepatitis C virus from replicating

...tive anti-HCV therapy. Using the OR6 cell culture assay system, they evaluated the anti-HCV activities of ... authors conclude. "Furthermore, our developed OR6 assay system will be useful for the time-saving screening of new anti-HCV reagents." ...

New strategy rapidly identifies cancer targets

...tudy of proteins. "We decided this more functional assay would get us to the disease-causing genes more rapidly," said Druker, who has been studying a group of cell-signaling proteins called tyrosine kinases for 20 years. Tyrosine kinases play a key role in many cancers. In healthy cells, they help form...

Novel therapy combinations gain ground in treating hepatitis

...elow the limits of detection of a highly sensitive assay after just eight days. All patients had undetectable HCV RNA by the end of 28 days. No patients experienced viral breakthrough at any time. "These data confirm the rapid and dramatic antiviral effects of VX-950. All subjects achieving undetectab...

Mushrooms as good an antioxidant source as more colorful veggies

...rgothioneine, present in mushrooms, using the ORAC assay and HPLC instrumentation, as part of her dissertat...pids carbohydrates and DNA. She adds, "The ORAC assay does not tell what happens in the human body but this assay is currently under investigation as to h...

AIDS-related cognitive impairment exists in two separate forms

...elp us treat patients accordingly," she said. The assay they used was developed by Illumina Inc., of San D...y. "Having batches of samples together in a single assay that can then be read automatically makes it easy for routine clinical use," she said. "The test has...

Markers of gene, protein, or micro-RNA activity predict outcome in prostate and colorectal cancers

...elp us treat patients accordingly," she said. The assay they used was developed by Illumina Inc., of San D...y. "Having batches of samples together in a single assay that can then be read automatically makes it easy for routine clinical use," she said. "The test has...

Measuring artery repair cells could become new heart disease test

... to become practical in human patients, we need an assay that is quick, reliable and accurate," said Povsic... in the bone marrow, so the team has extended this assay to detect levels of EPCs, which are progenitor cells in the blood which may repair one's arteries. ...

New test to detect rare proteins in blood

...nt assay). ELISA is a common immune-system-based assay that uses enzymes linked to an antibody or antigen...redict disease-specific survival. A more sensitive assay could more accurately allow treatment of humans with breast cancer and allow treatment more quickly ...

Scientists re-engineer a well-known antibiotic to counter drug resistance

... antibiotics and compared them in an antimicrobial assay against VanA, a strain of the bacteria that is highly resistant to treatment by glycopeptide antibiotics, including vancomycin and teicoplanin (a somewhat newer drug similar to vancomycin). Both showed a significant increase in binding ability-roughl...

Molecule by molecule, new assay shows real-time gene activity

...ted analysis to highly expressed genes. Xie's new assay addresses all three limitations. He and his colleagues melded a yellow fluorescent protein called Venus with Tsr, a hydrophobic membrane protein. The inclusion of the Tsr domain serves to anchor the fused protein to a cell's membrane, sidestepping th...

Minor mutations in avian flu virus increase chances of human infection

...oped microarray technology-hundreds of microscopic assay sites on a single small surface-the study showed t...the NIGMS. "This technology allows researchers to assay hundreds of carbohydrate varieties in a single experiment," Berg said. "The glycan microarray offers...

Contagious obesity? Identifying the human adenoviruses that may make us fat

...ovirus to cause human obesity by using an in vitro assay or even by animal testing, screening of the approximately 50 human adenoviruses might be accelerated, shortening the time required for vaccine formulation," Greenway wrote. "Human antibody prevalence in obese and lean human populations appears to be ...

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