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Transposase in Biological News

Methods for gene transfer in stem cells featured in Cold Spring Harbor Protocols

... integration and reliable long-term expression of a transgene. Sleeping Beauty transposon-based transfection is a two-component system consisting of a transposase and a transposon containing inverted repeat/direct repeat sequences that result in precise integration into a TA dinucleotide. The article is freely ...
Transposase in Biological Products

GPS -M Mutagenesis System from New England Biolabs

Description:... Mutagenesis System enables you to insert a transposable element of your own design into your target DNA by in vitro transposition. It uses TnsABC* transposase to insert a Tn7-based transposon randomly into the DNA target. Target DNA may be a plasmid, cosmid, BAC or purified chromosomal DNA, depending on ...
Company:New England Biolabs

EZ-Tn5 Transposase from EPICENTRE Biotechnologies

Description:... EZ-Tn5™ transposase is designed for use with our pMOD™ series of vectors as well as any EZ-Tn5™ transposon ....
Company:EPICENTRE Biotechnologies
Transposase in Biological Dictionary

Transposase

... transposase is an enzyme that binds to the ends of a ... mechanism. Aspects of the topic transposase are discussed in the following places at ... Prize in 1983. Most, if ... HyperMu™ MuA transposase is a hyperactive. enzyme that is at least 50 ...

Transposable element

... ... P-elements are transposable elements that carry genes for transposase activity ... shown to consist of transposable element sequences. ... to transposable elements and their classification ... Did You Know? transposase is an enzyme that catalyzes transposition. ... Why the insertion of a ...

Transposon mapping

... ... Transposon insertion points map to the following positions on the Ap HZ genome ... Physical maps of the Himar1 transposon and transposase plasmids. ... ...

Retrotransposon

... Furthermore, retrotransposon -induced mutations are relatively stable, because ... The integrase is the retrotransposon equivalent of the transposase of DNA ... Retrotransposon . Synonym(s) retroposon. Definition(s) ... retrotransposon DNA is first transcribed into an RNA template, then ...

Helix-turn-helix

... motif is detected in many transcription regulators (Brennan ... The helix – turn – helix motif of bacterial insertion sequence IS911 transposase is ... The second motif is a helix – turn – helix (HTH) (codons 25–44, Fig. Fig.1B)1 ... One cannot watch cells crawling, dividing, ...

Composite transposon

... Insertion Sequence (IS) elements in that it has protein coding DNA segments flanked by inverted, repeated sequences that can be recognized by transposase enzymes. A composite transposon , however, is flanked by two separate IS elements which may or may not be exact replicas. Instead ... A DNA ...

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