Cellectricon Launches World's First System for High Throughput RNAi Screening
... The world's first fully automated high throughput transfection system enables reagent-free delivery of genetic ... system on the market allowing for high throughput transfection of cells in culture directly in 384-well ... targeting the ion channel screening and transfection markets. The company utilizes state-of-the-art ...Farnesoid X receptor regulates cystathionase
... addresses this question. The research team led by Dr. Barbara Renga and her colleagues in the University of Perugia, Italy used luciferase transfection assay, Electrophoretic Mobility Shift Assay (EMSA), Chromatin Immunoprecipitation Assay and Real-Time PCR to confirm the role of FXR in the ...UCSF Team Closer to Creating Safe Embryonic-Like Stem Cells
... three viruses, each containing a transcription factor known to induce pluripotency. The transcription factor for c-myc was not included. The transfection involved a simple process in which the tiny microRNA molecules were mixed with a lipid, allowing them to pass through the cell membrane. By labeling ...UCSF team closer to creating safe embryonic-like stem cells
... three viruses, each containing a transcription factor known to induce pluripotency. The transcription factor for c-myc was not included. The transfection involved a simple process in which the tiny microRNA molecules were mixed with a lipid, allowing them to pass through the cell membrane. By labeling ...A potential approach to treatment of hepatitis B virus infection
... replication stress. They investigated whether the inhibition of DNA damage response by CF, TP and UCN01 or the restoration of p21 expression by p21 transfection or proteasome inhibition would lead to suppressed HBV replication. They set up a chronic HBV infection model by culturing hepatocyte HL7702 cells ...A potential route for human tumor gene therapy
... membranous ion exchange protein in gastric carcinoma cells, and the effects on the biological behaviors of gastric carcinoma cells. The purpose of transfection of the antisense NHE1 gene into the human gastric carcinoma cell line SGC-7901 is to investigate the effects of antisense therapy targeting the NHE1 ...Sigma-Aldrich Announces mirPremier(TM) microRNA Isolation Kit for miRNA Purification
... and AQUA peptides for protein level detection as well as an entire workflow of supporting reagents (cell culture, cell biology compounds and assays, transfection reagents, and much more). For more information, visit http://www.sigma.com/rnai . About Sigma-Aldrich: Sigma-Aldrich is a leading Life Science and ...Breakthrough Large Scale Transfection Technology Accelerates Cell-Based Assay Development
... Reagent-Free Preparative transfection Eliminates the Need to Engineer Stable Cell Lines ... today the introduction of its ITF Inline transfection System, a validated platform for preparative ... to liter volumes. MaxCyte's ITF Inline transfection System enables transient transfection of cell ...Artelis and Selexis Report Record Antibody Production Levels in CHO Cells of Over 31 Grams Per Liter
... the Selexis SURE Cell Line Development program, Selexis scientists can generate high-performance stable minipools in as little as 5 week following transfection or high-performance clonal cell lines 12 weeks after transfection. Like the Selexis Genetic Elements(TM) the Selexis SURE Cell Line Development ...Thermo Fisher Scientific Introduces Breakthrough in RNA-Interference Technology
... siRNA is a novel form of short- interfering RNA (siRNA) that is absorbed directly by cells without the use of conventional delivery methods such as transfection reagents, viruses or electroporation. Dharmacon Accell siRNA effectively silences genes in all cell types tested to date. RNAi is a powerful ...GENios Plus multi label reader
Description:... fluorescence labels. As a multi-detection system, GENios will also offer superior performance for additional applications such as expression and transfection studies (GFP and Luciferase), enzymatic assays (kinetic), toxicology or environmental investigations. For cell based assays, GENios allows you to ...Genios Multi-Detection Microplate Reader
Description:... fluorescence labels. As a multi-detection system, GENios will also offer superior performance for additional applications such as expression and transfection studies (GFP and Luciferase), enzymatic assays (kinetic), toxicology or environmental investigations. For cell based assays, GENios allows you to ...Methods for gene transfer in stem cells featured in Cold Spring Harbor Protocols
... ) provides a method for stable integration and reliable long-term expression of a transgene. Sleeping Beauty transposon-based transfection is a two-component system consisting of a transposase and a transposon containing inverted repeat/direct repeat sequences that result in precise ...Scientists eye secrets of retinal regeneration
... a gene-based method called "retinal transfection," to gain a more accurate picture of outer segment growth in rat retinas. "Basically, retinal transfection means introducing different genes into the eye to switch particular cellular functions on or off," Dr. Chuang explains. After a variety of these ...Buckyballs used as 'passkey' into cancer cells
... of pediatric cancer deaths," said Jianhua Yang. "Our findings are significant because neuroblastoma cells are well-known for their difficulty in transfection through the cell membrane." Barron is Rice's Charles W. Duncan Jr.-Welch Professor of Chemistry, professor of materials science and associate ...New culture method for hepatitis C virus uses primary hepatocytes and patient serum
... different HCV strains were added to the hepatocyte culture system, viral replication occurred and new virus particles were produced. In both transfection and infection models, virus particles were released in a cyclical manner, with bursts of virus produced every 10-14 days. This is similar to what has ...What's next for gene therapy? Plastic
... efficient release of the DNA for protein synthesis." Layman will deliver the talk, "Influence of macromolecular architecture on nucleic acid transfection (POLY 611)," at 4 p.m., Wednesday, Sept. 13, in the Marriott Salon 14/15 as part of the International Biorelated Polymers Symposium. Authors are ...Clontech Laboratories, Inc. Releases the Xfect(TM) and Xfect(TM) Stem Transfection Reagents
... 2009) release of the Xfect(TM) and Xfect(TM) Stem transfection reagents. These novel transfection reagents are based on biodegradable nanoparticles that permit superior transfection efficiency of plasmid DNA into mammalian cells. ...Altogen Biosystems Launches New Nanoparticle-based In Vivo Transfection Reagent
... ) announced launch of novel Nanoparticle-based transfection reagent for use in laboratories and research facilities for animal testing. transfection is one of the major laboratory methods used to ... to the engineering, marketing and manufacture of transfection reagents and kits for intracellular ...MaxCyte Debuts the STX Scalable Transfection System at the A/C/T Assays & Cellular Targets Meeting
... at MaxCyte, "The MaxCyte STX Scalable transfection System can be used to transfect primary cells, ... and operation of the MaxCyte STX Scalable transfection System. About MaxCyte MaxCyte specializes in ... expertise, MaxCyte has designed the MaxCyte STX transfection System expressly for rapid, scalable, and ...Senesco Signs a Supply Agreement for Polyplus-transfection's Delivery System
... and is reinvesting most of its revenues in research and development. transfection consists in introducing a gene or a small interfering RNA into cells. This ... The Strasbourg-based company is recognized as a leading innovator in the transfection market, with ISO 9001:2000 certification, exclusive licenses from the CNRS ...Silencer CellReady siRNA Transfection Optimization Kit from Ambion
Description:... Prior to siRNA library screening, optimal siRNA transfection parameters must be identified and verified for each cell type used. The Silencer CellReady siRNA transfection Optimization Kit, which was developed specifically for optimizing transfection conditions in 96 well plates, dramatically ...jetPEI-RGD + NaCl sol. from Polyplus Transfection
Description:... Epithelial and endothelial cells-directed transfection reagent : jetPEI-RGD is a RGD peptide-conjugated polyethylenimine which allows targeted efficient transfection of integrin-expressing epithelial and endothelial ... targeting. Integrin binding was shown to increase transfection of epithelial (HeLa) and fibroblast (MRC5) cells ...TransIT-siQUEST Transfection Reagent from Mirus Bio Corporation
Description:... findings, has developed TransIT-siQUEST transfection Reagent, which enables highly efficient siRNA transfection with significantly reduced levels of cell damage as compared to cationic-liposome based transfection reagents. Transfections are most effective when ...TransIT-siQUEST Transfection Reagent from Mirus Bio Corporation
Description:... findings, has developed TransIT-siQUEST transfection Reagent, which enables highly efficient siRNA transfection with significantly reduced levels of cell damage as compared to cationic-liposome based transfection reagents. Transfections are most effective when ...Label IT siRNA Tracker Cy3 Kit with TransIT-siQUEST Reagent from Mirus Bio Corporation
Description:... Localization Kit with TransIT-siQUEST transfection Reagent provides a straight forward approach to ... transfections in 24-well plates. TransIT-siQUEST transfection Reagent, which is included in the kit, is ... for the high efficiency, low toxicity transfection of siRNA in a variety of cell ....Label IT siRNA Tracker Biotin Kit with TransIT-siQUEST Reagent from Mirus Bio Corporation
Description:... Localization Kit with TransIT-siQUEST transfection Reagent provides a straight forward approach to ... transfections in 24-well plates. TransIT-siQUEST transfection Reagent, which is included in the kit, is ... for the high efficiency, low toxicity transfection of siRNA in a variety of cell ....Label IT siRNA Tracker TM Rhodamine Kit with TransIT-siQUEST Reagent from Mirus Bio Corporation
Description:... Localization Kit with TransIT-siQUEST transfection Reagent provides a straight forward approach to ... transfections in 24-well plates. TransIT-siQUEST transfection Reagent, which is included in the kit, is ... for the high efficiency, low toxicity transfection of siRNA in a variety of cell ....Label IT siRNA Tracker CX Rhodamine Kit with TransIT-siQUEST Reagent from Mirus Bio Corporation
Description:... Localization Kit with TransIT-siQUEST transfection Reagent provides a straight forward approach to ... transfections in 24-well plates. TransIT-siQUEST transfection Reagent, which is included in the kit, is ... for the high efficiency, low toxicity transfection of siRNA in a variety of cell ....Label IT siRNA Tracker Fluorescein Kit with TransIT-siQUEST Reagent from Mirus Bio Corporation
Description:... Localization Kit with TransIT-siQUEST transfection Reagent provides a straight forward approach to ... transfections in 24-well plates. TransIT-siQUEST transfection Reagent, which is included in the kit, is ... for the high efficiency, low toxicity transfection of siRNA in a variety of cell ....ProteoJuice Protein Transfection Reagent from Novagen
Description:... is to transfect the protein directly into the cell. ProteoJuice Protein transfection Reagent is an effective reagent for the transfection of intact functional protein into mammalian cells with minimal toxicity ...