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Transfection in Medical News

MaxCyte Presents a Panel Discussion With Industry Experts on the Challenges in Developing Cell-Based Screening Assays for Drug Discovery - LIVE Q&A to Follow

... Dr. Reardon reviewed the complexities of cell transfection technologies and challenged the webinar ... presentation of the MaxCyte(R) STX(TM) Scalable transfection System. Dr. Brady demonstrated how the MaxCyte STX technology enables the large scale transfection of primary cells, cell lines, and stem cells with ...

Cellectricon Launches World's First System for High Throughput RNAi Screening

... The world's first fully automated high throughput transfection system enables reagent-free delivery of genetic ... system on the market allowing for high throughput transfection of cells in culture directly in 384-well ... targeting the ion channel screening and transfection markets. The company utilizes state-of-the-art ...

MaxCyte to Present Targeted Cancer Immunotherapy Product at 50th Annual Meeting of the American Society of Hematology

... presentation is entitled, "A Highly Efficient, Clinically Applicable transfection Method to Redirect the Specificity of Immune Cells and Enhance Their ... expertise to the global leaders in cell-based therapies. MaxCyte's cell transfection technology platform enables the discovery, development, manufacturing ...

Farnesoid X receptor regulates cystathionase

... addresses this question. The research team led by Dr. Barbara Renga and her colleagues in the University of Perugia, Italy used luciferase transfection assay, Electrophoretic Mobility Shift Assay (EMSA), Chromatin Immunoprecipitation Assay and Real-Time PCR to confirm the role of FXR in the ...

UCSF Team Closer to Creating Safe Embryonic-Like Stem Cells

... three viruses, each containing a transcription factor known to induce pluripotency. The transcription factor for c-myc was not included. The transfection involved a simple process in which the tiny microRNA molecules were mixed with a lipid, allowing them to pass through the cell membrane. By labeling ...

UCSF team closer to creating safe embryonic-like stem cells

... three viruses, each containing a transcription factor known to induce pluripotency. The transcription factor for c-myc was not included. The transfection involved a simple process in which the tiny microRNA molecules were mixed with a lipid, allowing them to pass through the cell membrane. By labeling ...

A potential approach to treatment of hepatitis B virus infection

... replication stress. They investigated whether the inhibition of DNA damage response by CF, TP and UCN01 or the restoration of p21 expression by p21 transfection or proteasome inhibition would lead to suppressed HBV replication. They set up a chronic HBV infection model by culturing hepatocyte HL7702 cells ...

A potential route for human tumor gene therapy

... membranous ion exchange protein in gastric carcinoma cells, and the effects on the biological behaviors of gastric carcinoma cells. The purpose of transfection of the antisense NHE1 gene into the human gastric carcinoma cell line SGC-7901 is to investigate the effects of antisense therapy targeting the NHE1 ...

Sigma-Aldrich Announces mirPremier(TM) microRNA Isolation Kit for miRNA Purification

... and AQUA peptides for protein level detection as well as an entire workflow of supporting reagents (cell culture, cell biology compounds and assays, transfection reagents, and much more). For more information, visit http://www.sigma.com/rnai . About Sigma-Aldrich: Sigma-Aldrich is a leading Life Science and ...

The new source of islet cells

... to diabetic patients. The article describes the differentiation of rat pancreatic ductal epithelial cells into insulin-producing cells by the transfection of PDX-1. In recent years, though great efforts have been made to differentiate embryonic stem cells, pancreatic ductal epithelial multipotent ...
Transfection in Medical Technology

Breakthrough Large Scale Transfection Technology Accelerates Cell-Based Assay Development

... Reagent-Free Preparative transfection Eliminates the Need to Engineer Stable Cell Lines ... today the introduction of its ITF Inline transfection System, a validated platform for preparative ... to liter volumes. MaxCyte's ITF Inline transfection System enables transient transfection of cell ...

Latest Cell Publication Describes the Novel Kinase Regulators Using OriGene's Functional Kinome Collection

... now offers the convenient Kinome GFC (Genome wide Full-length cDNA) transfection Array. About OriGene Technologies Since 1996, OriGene has been ... source of over 33,000 human full-length cDNA clones suitable for transfection and protein expression and TrueORF clones of over 25,000 tagged human ORF ...

Artelis and Selexis Report Record Antibody Production Levels in CHO Cells of Over 31 Grams Per Liter

... the Selexis SURE Cell Line Development program, Selexis scientists can generate high-performance stable minipools in as little as 5 week following transfection or high-performance clonal cell lines 12 weeks after transfection. Like the Selexis Genetic Elements(TM) the Selexis SURE Cell Line Development ...

Thermo Fisher Scientific Introduces Breakthrough in RNA-Interference Technology

... siRNA is a novel form of short- interfering RNA (siRNA) that is absorbed directly by cells without the use of conventional delivery methods such as transfection reagents, viruses or electroporation. Dharmacon Accell siRNA effectively silences genes in all cell types tested to date. RNAi is a powerful ...

Newly Published Phase II Study Shows the Investigational Drug Vandetanib Prolongs Progression-Free Survival in Patients with Advanced Non-Small Cell Lung Cancer (NSCLC)

... involved in tumor growth and spread. Tumor cells are targeted through inhibition of epidermal growth factor receptor (EGFR) and REarranged during transfection (RET) tyrosine kinases, while tumor blood supply is targeted through inhibition of vascular endothelial growth factor receptor (VEGFR) tyrosine ...
Transfection in Medical Products

GENios Plus multi label reader

Description:... fluorescence labels. As a multi-detection system, GENios will also offer superior performance for additional applications such as expression and transfection studies (GFP and Luciferase), enzymatic assays (kinetic), toxicology or environmental investigations. For cell based assays, GENios allows you to ...
Company:Tecan

Genios Multi-Detection Microplate Reader

Description:... fluorescence labels. As a multi-detection system, GENios will also offer superior performance for additional applications such as expression and transfection studies (GFP and Luciferase), enzymatic assays (kinetic), toxicology or environmental investigations. For cell based assays, GENios allows you to ...
Company:Tecan
Transfection in Biological News

Methods for gene transfer in stem cells featured in Cold Spring Harbor Protocols

... ) provides a method for stable integration and reliable long-term expression of a transgene. Sleeping Beauty transposon-based transfection is a two-component system consisting of a transposase and a transposon containing inverted repeat/direct repeat sequences that result in precise ...

Scientists eye secrets of retinal regeneration

... a gene-based method called "retinal transfection," to gain a more accurate picture of outer segment growth in rat retinas. "Basically, retinal transfection means introducing different genes into the eye to switch particular cellular functions on or off," Dr. Chuang explains. After a variety of these ...

Buckyballs used as 'passkey' into cancer cells

... of pediatric cancer deaths," said Jianhua Yang. "Our findings are significant because neuroblastoma cells are well-known for their difficulty in transfection through the cell membrane." Barron is Rice's Charles W. Duncan Jr.-Welch Professor of Chemistry, professor of materials science and associate ...

New culture method for hepatitis C virus uses primary hepatocytes and patient serum

... different HCV strains were added to the hepatocyte culture system, viral replication occurred and new virus particles were produced. In both transfection and infection models, virus particles were released in a cyclical manner, with bursts of virus produced every 10-14 days. This is similar to what has ...

What's next for gene therapy? Plastic

... efficient release of the DNA for protein synthesis." Layman will deliver the talk, "Influence of macromolecular architecture on nucleic acid transfection (POLY 611)," at 4 p.m., Wednesday, Sept. 13, in the Marriott Salon 14/15 as part of the International Biorelated Polymers Symposium. Authors are ...
Transfection in Biological Technology

University of Lodz Invests in the Unique Cellaxess(R)HT System for Research on Multidrug Resistance Genes in Cancer

... the world's first system for high throughput transfection enabling reagent-free delivery of any genetic ... very significant for a technology enabling high transfection efficiency and viability in biologically relevant ... the Cellaxess(R)HT System enables high throughput transfection in genomic screening and the Dynaflow(R)HT System ...

Clontech Laboratories, Inc. Releases the Xfect(TM) and Xfect(TM) Stem Transfection Reagents

... 2009) release of the Xfect(TM) and Xfect(TM) Stem transfection reagents. These novel transfection reagents are based on biodegradable nanoparticles that permit superior transfection efficiency of plasmid DNA into mammalian cells. ...

MaxCyte Introduces the GT(TM) Flow Transfection System for Application with Autologous and Allogeneic Stem Cell Therapies

... today the introduction of its GT(TM) Flow transfection System at the 4th Annual Stem Cell Summit to be held in New York, NY. The MaxCyte GT(TM) Flow transfection System is a validated, scalable technology for ... a US FDA Master File, enables rapid and efficient transfection of any primary cell or cell line with >90% ...

The MaxCyte(R) STX(TM) Scalable Transfection System to be Presented at the Rapid Fire Innovation Session at LabAutomation2009

... presentation of the MaxCyte(R) STX(TM) Scalable transfection System at LabAutomation2009 in the Rapid Fire ... to the uniqueness of MaxCyte's large scale transfection technology. Using the MaxCyte STX, more than ten ... has designed the MaxCyte(R) STX(TM) Scalable transfection System expressly for rapid, scalable, and ...

MaxCyte Hosts Live Discussion with Industry Experts on the Challenges in Developing Cell-Based Screening Assays for Drug Discovery

... Dr. Reardon will review the complexities of cell transfection technologies and challenge the webinar ... MaxCyte STX technology enables the large scale transfection of primary cells, cell lines, and stem cells ... and operation of the MaxCyte STX Scalable transfection System. A recording of the webinar will be ...

Altogen Biosystems Launches New Nanoparticle-based In Vivo Transfection Reagent

... ) announced launch of novel Nanoparticle-based transfection reagent for use in laboratories and research facilities for animal testing. transfection is one of the major laboratory methods used to ... to the engineering, marketing and manufacture of transfection reagents and kits for intracellular ...

The MaxCyte STX Scalable Transfection System to be Launched at the SBS Symposium on Cell-Based Assays

... it will be launching the MaxCyte STX Scalable transfection System at the Society for Biomolecular Sciences ... "We believe that the MaxCyte STX Scalable transfection System addresses many of these challenges, ... MaxCyte STX technology enables the large scale transfection of primary cells, cell lines, and stem cells ...

MaxCyte Debuts the STX Scalable Transfection System at the A/C/T Assays & Cellular Targets Meeting

... at MaxCyte, "The MaxCyte STX Scalable transfection System can be used to transfect primary cells, ... and operation of the MaxCyte STX Scalable transfection System. About MaxCyte MaxCyte specializes in ... expertise, MaxCyte has designed the MaxCyte STX transfection System expressly for rapid, scalable, and ...

Sigma-Aldrich Introduces N-TER Nanoparticle siRNA Transfection System for Delivery of siRNA Into Difficult-to-Transfect Cell Types

... release of the N-TER(TM) Nanoparticle siRNA transfection System. N-TER allows researchers to ... reagents. "Traditional lipid-based siRNA transfection reagents exhibit a number of drawbacks, including ... these historic limitations and enables siRNA transfection to be performed in a wider variety of model ...

Senesco Signs a Supply Agreement for Polyplus-transfection's Delivery System

... and is reinvesting most of its revenues in research and development. transfection consists in introducing a gene or a small interfering RNA into cells. This ... The Strasbourg-based company is recognized as a leading innovator in the transfection market, with ISO 9001:2000 certification, exclusive licenses from the CNRS ...

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Transfection in Biological Products

Silencer CellReady siRNA Transfection Optimization Kit from Ambion

Description:... Prior to siRNA library screening, optimal siRNA transfection parameters must be identified and verified for each cell type used. The Silencer CellReady siRNA transfection Optimization Kit, which was developed specifically for optimizing transfection conditions in 96 well plates, dramatically ...
Company:Ambion

jetPEI-RGD + NaCl sol. from Polyplus Transfection

Description:... Epithelial and endothelial cells-directed transfection reagent : jetPEI-RGD is a RGD peptide-conjugated polyethylenimine which allows targeted efficient transfection of integrin-expressing epithelial and endothelial ... targeting. Integrin binding was shown to increase transfection of epithelial (HeLa) and fibroblast (MRC5) cells ...
Company:Polyplus Transfection

TransIT-siQUEST Transfection Reagent from Mirus Bio Corporation

Description:... findings, has developed TransIT-siQUEST transfection Reagent, which enables highly efficient siRNA transfection with significantly reduced levels of cell damage as compared to cationic-liposome based transfection reagents. Transfections are most effective when ...
Company:Mirus Bio Corporation

TransIT-siQUEST Transfection Reagent from Mirus Bio Corporation

Description:... findings, has developed TransIT-siQUEST transfection Reagent, which enables highly efficient siRNA transfection with significantly reduced levels of cell damage as compared to cationic-liposome based transfection reagents. Transfections are most effective when ...
Company:Mirus Bio Corporation

Label IT siRNA Tracker Cy3 Kit with TransIT-siQUEST Reagent from Mirus Bio Corporation

Description:... Localization Kit with TransIT-siQUEST transfection Reagent provides a straight forward approach to ... transfections in 24-well plates. TransIT-siQUEST transfection Reagent, which is included in the kit, is ... for the high efficiency, low toxicity transfection of siRNA in a variety of cell ....
Company:Mirus Bio Corporation

Label IT siRNA Tracker Biotin Kit with TransIT-siQUEST Reagent from Mirus Bio Corporation

Description:... Localization Kit with TransIT-siQUEST transfection Reagent provides a straight forward approach to ... transfections in 24-well plates. TransIT-siQUEST transfection Reagent, which is included in the kit, is ... for the high efficiency, low toxicity transfection of siRNA in a variety of cell ....
Company:Mirus Bio Corporation

Label IT siRNA Tracker TM Rhodamine Kit with TransIT-siQUEST Reagent from Mirus Bio Corporation

Description:... Localization Kit with TransIT-siQUEST transfection Reagent provides a straight forward approach to ... transfections in 24-well plates. TransIT-siQUEST transfection Reagent, which is included in the kit, is ... for the high efficiency, low toxicity transfection of siRNA in a variety of cell ....
Company:Mirus Bio Corporation

Label IT siRNA Tracker CX Rhodamine Kit with TransIT-siQUEST Reagent from Mirus Bio Corporation

Description:... Localization Kit with TransIT-siQUEST transfection Reagent provides a straight forward approach to ... transfections in 24-well plates. TransIT-siQUEST transfection Reagent, which is included in the kit, is ... for the high efficiency, low toxicity transfection of siRNA in a variety of cell ....
Company:Mirus Bio Corporation

Label IT siRNA Tracker Fluorescein Kit with TransIT-siQUEST Reagent from Mirus Bio Corporation

Description:... Localization Kit with TransIT-siQUEST transfection Reagent provides a straight forward approach to ... transfections in 24-well plates. TransIT-siQUEST transfection Reagent, which is included in the kit, is ... for the high efficiency, low toxicity transfection of siRNA in a variety of cell ....
Company:Mirus Bio Corporation

ProteoJuice Protein Transfection Reagent from Novagen

Description:... is to transfect the protein directly into the cell. ProteoJuice Protein transfection Reagent is an effective reagent for the transfection of intact functional protein into mammalian cells with minimal toxicity ...
Company:Novagen

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Transfection in Biological Definition

Molecular biology

... cells, is called transfection. Several different transfection techniques are available, including calcium phosphate transfection, liposome transfection , and proprietary transfection reagents such as Fugene. DNA can also be ...
Transfection in Biological Dictionary

Transient transfection

... transfection is the process of introducing nucleic acids ...DNA... Encyclopedia information on Transient transfection ... In transient transfection , the gene introduced can be lost from the ...

Transfection

... transfection is the process of introducing nucleic acids ... often used to describe virus-mediated ... transfection n. Infection of a cell with purified viral ... is focused on developing innovative reagents for transfection , gene expression, Gene Silencing,RNA ...

Transcapsidation

... transcapsidation approach consisted of the transfection of pairwise ... Transcapsidation between ... transcapsidation approach consisted of the transfection of pairwise ... virus groups determines ... transcapsidation approach consisted of the transfection of pairwise ... Transcapsidation ,biological ...

Stable transfection

... transfection is the process of introducing nucleic acids ... to describe virus-mediated ... Stable transfection . Transient and Stable transfection ... effect is a stable or transient ...

CDNA array

... TrueORF cDNA Clones. Destination Vector. TrueClone Human Collection ... transfection Array - 704 transfection ready cDNA plasmids ... High throughput over expression assay for ...

SiRNA

... Predesigned siRNA and siRNA libraries designed to reduce off-target effects and increase RNAi performance. Custom siRNA synthesis services and transfection reagents. - - Online guide for performing RNAi experiments using synthetic siRNA molecules. ... We used to select siRNA sequences with TT ...

Reporter Gene

... Protein (GFP) Reporter Gene System ... Human Growth Hormone (hGH) Reporter Gene System ... Products > Genetic Reporters & transfection > Applications of Reporter Gene Assays ... Using Bioluminescent Reporter Genes to Optimize shRNA Target Sites for ... Detect reporter ...

In vivo

... annual sketch comedy show. ... This is a very special year for In Vivo as we will be celebrating the show's 30th anniversary! ... In Vivo transfection Agent :: siRNA and DNA Delivery Invivo ... siRNA and plasmid DNA in vivo delivery reagent. Modes of administration: ... What is in vivo ? ...

Genomic DNA

... Human genomic DNA matched pair products for tumor specific gene & tumor ... DNA Kits. Full Length cDNA Clones. Genomic DNA . GFC transfection Arrays. Matched Pair cDNA ... PCR-Ready Genomic DNA - Plant Normal Tissue: Arabidopsis. BioChain. 10 µg ... Apple Genomic DNA . Zyagen ...

Electroporation

... vaccine therapeutics and delivery systems, ... mechanism of electroporation is not ... The world leader in the field of electroporation and transfection offering an extensive line of products and services for the laboratory research market. ECM 830 system for electroporation of mammalian cells ...

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