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ribonuclease at Biology News

ribonuclease at Biology Products

DNase I (RNase-free) (2 U/l) from Ambion

...ted cells which may contain plasmid DNA), used for ribonuclease protection assays, cDNA library contraction, and RT-PCR. DNase I requires bivalent cations (Mg2+ and Ca2+ at approximately 5 mM and 0.5 mM, respectively) for maximal activity and has a pH optimum of 7.8....

RNase Inhibitor (Cloned) from Ambion

...nt in many tissues and cell types. Addition of the ribonuclease inhibitor has been shown to be useful whenever the integrity of RNA must be maintained such as in the preparation of cDNA by reverse transcription, in vitro RNA transcription and in in vitro protein synthesis, and RNA isolation and preparation of RNa...

ANTI-RNase from Ambion

ANTI-RNase is a protein ribonuclease inhibitor that noncovalently binds and inactivates...r CL3. ANTI-RNase is distinct from human placental ribonuclease inhibitor in that it has a more robust interaction with RNase and does not release active RNase in t...

SUPERaseIn from Ambion

... The most widely used RNase inhibitor is placental ribonuclease inhibitor (RIP). SUPERaseIn is distinct from RIP i...nhibits a broader range of RNases. While placental ribonuclease inhibitors are effective only against RNase A type enzymes, SUPERaseIn also inhibits RNase 1 and T1....

StabMRT Midi Kit from RNAworks

...esis, Agilent Bioanalyser 2100, mass spectrometry, ribonuclease protection assays and Northern blotting. Protocols on line on RNAworks website A free MRT-RNA sample is available on request from RNAworks....

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ribonuclease at Biology Technology

Reliable Assessment of Micronuclei for Genotoxicity Testing in Mice or Rats

...0 When fixed blood cells are treated with ribonuclease and antitransferrin receptor antibody (FITC-conjugate), and then stained with propidium iodide, normochromatic erythrocytes (NCEs) and RETswith and without MNare clearly resolved ( Figure 1 ). ...

RNase Protection Assay (RPA) Using DIG-Labeled RNA Probes

The ribonuclease protection assay (RPA) is a highly sensitiveand specific method for the detection and quantitationof mRNA species. The assay was made possibleby the discovery and characterization of bacteriophageencodedDNA-dependent RNA polymerases (SP6, T7,and T3)....

Improve RNA Isolation for Array Analysis and More

... yeast, bacteria), or samples that are high in ribonuclease or other contaminants (e.g. spleen, pancreas, plant and neural tissues). RiboPure is the method of choice for preparing RNA for use in microarray analysis or other procedures that ...

Detecting miRNA & siRNA

...d excess RNA probe were removed by a brief ribonuclease digestion. Protected target RNA fragments were rec...sing a reagent that simultaneously inactivates the ribonuclease and precipitates the nucleic acid. The protected RNA was then resuspended with gel l...

Detecting Attomole Amounts of Small RNA

...probe is then removed by a rapid ribonuclease digestion step. The hybridized, ...step technology for simultaneous ribonuclease inactivation and nucleic acid precipitation. RNA samples are then resuspended with...

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ribonuclease at Biology Dictionary

SSCP

...ngle- strand conformation polymorphism RPA: ribonuclease -protection assay RFLP : restriction fragment length polymorphism PCR : polymerase chain re action amplification with mutation -specific primer s ... Full article >>> Methods such as single strand conformation polym...

S1 nuclease

...es from the end of the substrate molecule . In ribonuclease ( RNase ), the substrate is RNA . In the S1 nuclease , ... Full article >>> In the S1 nuclease , the substrate is single- strand ed DNA or RNA . Nucleic acids . The two nucleic acids , deoxyribonucleic acid ( DNA ) and ...

RNase

...e of RNase s. Full article >>> In ribonuclease ( RNase ), the substrate is RNA . In the S1 nuclease , the substrate is single- strand ed DNA or RNA . Nucleic acids . Full article >>>...

Ribonuclease

...m RNA is trans cribed before being destroyed by ribonuclease s. Full article >>> Ribonuclease ...ransfer RNA (t RNA ) molecule s. In bacteria , ribonuclease P is a hetero dimer containing ... Full article >>> RIBONUCLEASE ( RNase ) - An ...

Conformation

...ngle- strand conformation polymorphism RPA: ribonuclease -protection assay RFLP : restriction fragment length polymorphism PCR : polymerase chain re action amplification with mutation -specific primer s ... Full article >>> Contrast with intermediary metabolism secondary st...

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ribonuclease at Medicine News

Brain Tumor Drug in Frogs A New Report

...reatment of the future.Amphinase is a version of a ribonuclease enzyme that has been isolated from the oocytes (eg... in the future.Amphinase is the second anti-tumour ribonuclease to be isolated by Alfacell Corporation from Rana pipiens oocytes. The other, ONCONASE(R) (ranpirna...
ribonuclease at Medicine Products
ribonuclease at Medicine Technology

In Vitro and In Vivo Data Show Alfacell's Onocanse is Active Against Naive and Chemoresistant Neuroblastoma Cells

...c product candidate basedon Alfacell's proprietary ribonuclease (RNase) technology. Anatural protein isolated from the leopard frog, ONCONASE has beenshown in the laboratory and clinic to target cancer cells whilesparing normal cells. ONCONASE triggers apoptosis, the naturaldeath of cells, via multiple molecular ...

Onconase has Potential as Chemopreventive Agent in Mesothelioma, Reports World-Renowned Mesothelioma Researcher at AACR Annual Meeting

...c product candidate basedon Alfacell's proprietary ribonuclease (RNase) technology. Anatural protein isolated from the leopard frog, ONCONASE has beenshown in the laboratory and clinic to target cancer cells whilesparing normal cells. ONCONASE triggers apoptosis, the naturaldeath of cells, via multiple molecular ...

Preclinical Data Show Alfacell's Onconase Inhibits Tumor Growth of Non-Small Cell Lung Cancer, Breast Cancer Cells

...c product candidate basedon Alfacell's proprietary ribonuclease (RNase) technology. Anatural protein isolated from the leopard frog, ONCONASE has beenshown in the laboratory and clinic to target cancer cells whilesparing normal cells. ONCONASE triggers apoptosis, the naturaldeath of cells, via multiple molecular ...

Researchers Identify Intracellular Pathway of Alfacell's Onconase

...c product candidate basedon Alfacell's proprietary ribonuclease (RNase) technology. Anatural protein isolated from the leopard frog, ONCONASE has beenshown in the laboratory and clinic to target cancer cells whilesparing normal cells. ONCONASE triggers apoptosis, the naturaldeath of cells, via multiple molecular ...

In vivo Study Results Demonstrating Potential of Alfacell's Onconase for Treatment of Non-Small Cell Lung Cancer Published in Anticancer Research

...n-class therapeutic based on Alfacell'sproprietary ribonuclease (RNase) technology. A natural proteinisolated from the leopard frog, ONCONASE has been shown in thelaboratory and clinic to target cancer cells while sparing normalcells. ONCONASE triggers apoptosis, the natural death of cells, viamultiple molecular ...

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